Inflammation resolution is an active process that functions to restore tissue homeostasis. Phagocytic clearance of apoptotic leukocytes (efferocytosis) at sites of inflammation plays an important role in inflammation resolution by inducing remarkable macrophage phenotypic plasticity. The M1/M2 nomenclature provides a basic classification system to distinguish proinflammatory (M1) from antiâinflammatory/resolving macrophages (M2/Mres). Plasminogen (Plg) is synthesized by the liver and is the proenzyme of the broadâspectrum serine protease, plasmin (Pla), which is the major enzyme responsible for fibrinolysis. The participation of the Plg system in the productive phase of inflammation is well known, but the involvement of the Plg system in the resolving phase of inflammation is just emerging. Here we investigated the effects of deletion of Plg and the Plg receptor, PlgâRKT, separately, in the context of resolution of inflammation in vivo. Plgâ/â and PlgâRKT â/â mice and their respective wild type littermates were challenged by intrapleural injection of LPS (250ng/mouse, i.pl.) or PBS and cells present in the pleural cavity were harvested at 4, 8, 24 and 48 hr and processed for total and differential leukocyte counts and flow cytometric analyses. In this model of pleurisy there is a timeâdependent influx of leukocytes into the pleural cavity. The number of neutrophils and M1 macrophages peaks at 8 hr, and decreases at 48 hr. There is a significant increase in mononuclear cell recruitment into the pleural cavity that coincides with the resolution phase of inflammation and with prevalence of M2 and Mres macrophages at 48hr. In the current study, in the setting of LPSâinduced inflammation, increased numbers of M1 macrophages were present in Plgâ/â mice 8h after LPS challenge without changes in number of M2 or Mres macrophages, as compared to Plg+/+ littermates [Plg+/+ = 155 ± 39 and Plgâ/â = 301 ± 57 Ă 103 M1 macrophages/cavity (P < 0.05, n=6)]. There were no genotypeâdependent effects on the number of neutrophils recruited to the pleural cavity at all time points evaluated. At 48 hr the total number of mononuclear cells recruited to the pleural cavity was significantly decreased in both Plgâ/â and PlgâRKT â/â mice, relative to their respective wild type littermate controls [Plg+/+ = 3,432 ± 335 and Plgâ/â = 2,377 ± 242 (P < 0.01, n=5); PlgâRKT +/+ = 2,780 ± 341 and PlgâRKT â/â = 1,725 ± 95 Ă 103 mononuclear cells/cavity (P < 0.05, n=6)]. Additionally, the frequency of the M2 marker, CD206, and Annexin A1 (both important for clearance of apoptotic cells) was decreased in Plgâ/â macrophages 48 hr after LPS injection. To explore the roles of Plg and PlgâRKT in efferocytosis, mice were injected with zymosan intraperitoneally, followed by an intraperitoneal injection of apoptotic human neutrophils labeled with carboxyfluorescein succinimidyl ester (CFSE). Significantly decreased efferocytosis of apoptotic neutrophils by Plgâ/â macrophages and by PlgâRKTâ/â macrophages, compared with their wild type littermate controls was observed [Plg+/+ = 20.16 ± 3.57 and Plgâ/â = 9.04 ± 3.50 (P < 0.05, n=5); PlgâRKT +/+ = 18.11 ± 4.57 and PlgâRKTâ/â = 5.80 ± 2.90 frequency of total cells/cavity (P < 0.05, n=6)]. These results suggest that Plg regulates macrophage polarization and efferocytosis of apoptotic cells, thereby contributing to the resolution of inflammation and that these effects are mediated by the Plg receptor, PlgâRKT.Support or Funding InformationCAPES (Coordenação de Aperfeiçoamento de Pessoal de NĂvel Superior, Brazil), National Institutes of Health (grant HL 081046 to L.A.M.) and by Merit Review Award #5I01BX002026 from the U.S. Department of Veterans Affairs (to R.J.P.).This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.