Resistance Of Red Blood Cells Research Articles

Erythroblastic anaemia with kyphosis and cirrhosis of liver

A fatal case of moderate anaemia with a high erythroblastic reaction, Mongolism, osseous changes, increased resistance of red blood cells, osteochondral dystrophy of a dorsal vertebra with kyphosis and cirrhosis of the liver with ascites is described.

The resistance of red blood cells to hemolysis in hypotonic solutions of sodium chloride

The resistance of red blood cells to hemolysis in hypotonic solutions of sodium chloride

Effects of Anesthetics on Osmotic Resistance of Erythrocytes: I. Ether and Chloroform.

Widely conflicting opinion exists with regard to the effect of anesthetics on the resistance of red blood cells to hypotonic saline solutions. Since little of this is based on quantitative data, it was thought worth while to restudy the problem, using the quantitative method introduced by Simmel,1 in which actual count is made of the number of cells remaining after uniform periods of contact with different grades of hypotonic solutions. The results are conveniently expressed as percentages of the total number of erythrocytes per cubic millimeter, where Hayem's solution has been used as the diluent. The normal variations likely to be encountered by this method have been studied by Leake and Pratt.2Dogs were used in all experiments. Blood was drawn by syringe from the femoral artery, through the intact skin. Ether or chloroform was administered by the drop method, and further samples of blood were drawn at various intervals up to one hour after the induction of the surgical stage of anesthesia. These were p...

THE RESISTANCE OF NORMAL HUMAN ERYTHROCYTES TO HYPOTONIC SALINE SOLUTIONS

This report is made for the purpose of calling attention to the extremely wide variations in the normal resistance of red blood cells to hypotonic saline solutions. These variations are more striking when the fragility of the erythrocytes is tested quantitatively, according to Simmel's technic. 1 Briefly, the method is as follows: The standard Simmel's fluid contains 8.2 gm. of sodium chlorid, 0.2 gm. of potassium chlorid, 0.2 gm. of magnesium chlorid, 0.2 gm. of calcium chlorid, 0.2 gm. of sodium acid phosphate and 0.05 gm. of sodium bicarbonate in 1 liter of water. This solution is isotonic with whole blood, is buffered to approximately the same degree as whole blood, has the same p H, and the salts are in approximately the same proportions as in whole blood. This standard fluid is diluted with water to 70, 60, 50 and 40 per cent., respectively. A red blood cell count

A Study of Saponin Hemolysis

Summary The hemolytic action of saponin or sapotoxin is, to a certain extent, inhibited in a nonelectrolyte medium. This phenomenon is, perhaps, attributable to the increase of viscosity of the medium, which makes the diffusion of saponin into red blood cells the more difficult. Ions of various salts favor saponin hemolysis even in higher concentration, except (NH4)2SO4, which can alter the red blood cells in higher concentration. BaCl2 and CaCl2 are indifferent. The resistance of red blood cells against saponin is different in the different species of animal. I desire to express my indebtedness to Professor S. Mita for his kind direction and encouragement during my experiments.

Variations in resistance of red blood cells in sheep

In the course of complement fixation work we have noticed that the specimens of blood cells obtained from different sheep under exactly similar conditions occasionally show marked differences in their susceptibility to laking by specific lytic serum. When this was first noticed we were using two sheep as a source of blood, and as the sheep whose cells were more highly resistant was one which had been bled repeatedly and profusely it was natural to attribute the increased resistance of the cells to this.We determined to investigate this question and also to find out whether the increased resistance was a specific resistance to laking by amboceptor and complement or was a general resistance such as would be shown by an increased resistance to laking by hypotonic salt solution, and also whether the sera of different sheep show similar differences in their tonicity.Three sheep were used:—T—an apparently normal young male sheep, newly obtained from the dealer.F—a sheep which had been kept in the laboratory for...

On the specific acquired resistance of red blood cells

It is a well known fact that the serum plays a preponderating role in the immune reactions of animals. On the other hand, it has been amply shown that the immune characters of the serum may be in no proportion to the immunity of the organism as a whole. There must therefore be an additional factor, hypothetically designated by Behring as cytogenetic immunity. Of this type of immunity there is the following experimental evidence. In 1898, Kossel stated that if an animal were injected with eel serum, its washed red cells manifested resistance to the hemolytic action of the latter in vitro; a fact confirmed by Camus and Gley, and by Tshistovitch. In 1908, it was shown by Morawitz and Pratt that in animals injected with phenylhydrazin, the red cells became resistant to all hemolytic agents. This they showed to be not an immune reaction, but the direct result of the chemical action of the drug on the red cells.