Mancozeb Residues Research Articles

Electrolyzed water combined with ozone treatment for efficient removal of mancozeb residues from grapes.

Existing cleaning methods mainly focus on removing free-state pesticides. However, mancozeb can bind to the wax layer of grapes, forming bound-state residues that are difficult to remove. This study aims to develop an effective cleaning strategy to eliminate both free and bound mancozeb residues from grapes. Compared with the untreated mancozeb aqueous solution, the concentration of free mancozeb significantly decreased (p<0.05) after treatment with ozonated water (OW), electrolyzed water (EW), and their combination (OW+EW) for 60min. The combined treatment synergistically promoted mancozeb degradation, thus reducing its half-life to 38% and 75% of that observed when OW and EW were used alone, respectively. To investigate the effect of the waxy layer on mancozeb removal, oleanolic acid (OLA) was selected as a representative component. The binding effect of OLA limited the degradation of mancozeb in OW and EW, extending its half-life by 1.27 and 1.20 times, respectively. Density functional theory elucidated the mechanism by which the binding of OLA affects the degradation of mancozeb. Interestingly, the decomposition of mancozeb in OW + EW was almost unaffected by the introduction of OLA, indicating that the combined treatment could effectively remove bound-state mancozeb. The combined treatment was then successfully applied to remove mancozeb from grapes. After exposure to OW + EW for 10 min, the removal efficiency of mancozeb reached up to 80.61% with minimal risks of ethylene thiourea formation. There was no obvious change in the surface color of grapes after treatment. The findings provide valuable guidance for removing mancozeb from fresh fruits rich in waxy coatings.

Impact of compost and biochar from agricultural waste on reducing cadmium concentration and mancozeb residue in soil

The negative impact of excessive exposure to agrochemicals in shallot cultivation causes environmental pollution and human health. Biochar has the potential to absorb agrochemical contamination. This research aimed to investigate the effect of providing compost and biochar from agricultural waste on land quality, reducing the concentration of heavy metal cadmium (Cd) and mancozeb pesticide residues in soil and products in shallot. The experiment was carried out in shallot fields in Ngurensiti Village, Pati Regency, Central Java Province. Four different treatments, including combinations of biochar and compost, were applied, along with conventional controls. Data were analyzed using the F test (ANOVA) and Tukey's test using the Minitab statistical program version 16.0. The research showed that using biochar made from sugarcane bagasse, rice husk, corncob, and compost helped more soil bacteria grow and lowered Cd and mancozeb concentrations. In addition, treatment with biochar from sugarcane bagasse waste showed a decrease in Cd and mancozeb concentrations and a more significant increase in bacterial populations compared to other treatments (rice husk biochar and corncob biochar). Although there was a slight increase in Cd concentration in shallot leaves post-treatment, Cd levels in shallot bulbs remained within safe limits. This study shows that using biochar and compost from agricultural waste effectively improves soil quality, reduces heavy metal pollution, and lowers pesticide levels to support sustainable agriculture and protect people's health.

Microwave-Assisted “One-Pot” Acidolysis and Extraction for the Rapid Determination of Mancozeb in Fruit and Vegetable Samples

Mancozeb is an extensively consumed fungicide, which often leaves high residue levels on agricultural products. The conventional method for detecting mancozeb involves a time-consuming process using gas chromatography (GC) after a 2-hour water-bath acidolysis, resulting in low efficiency and recovery rates. This study developed a rapid method for detecting mancozeb in fruits and vegetables using microwave-assisted acidolysis and extraction coupled with GC analysis. Mancozeb underwent “one-pot” acidolysis to generate CS2 gas and was subsequently extracted from samples using microwave treatment, requiring only 50 seconds of pretreatment time. The average recoveries of mancozeb ranged from 81% to 112%. The limit of detection and limit of quantification were 0.003 and 0.01 mg kg−1, respectively. The scanning electron microscope imaging showed that strong cell crumpling after microwave treatment improved the acidolysis rate significantly, where the acidolysis rate was 91.8% for mancozeb. In addition, this method is rapid, simple, and precise for detecting residues of mancozeb and other dithiocarbamate fungicides.

Residue behaviour and health risk assessment of chlorpyrifos and mancozeb in apple fruits and soil.

Mancozeb residue estimation was done using second derivative ultraviolet spectroscopy by Shimadzu ultraviolet-visible spectrophotometer, and chlorpyrifos was estimated by QuEChERS technique using GC-FPD. The persistence for chlorpyrifos was carried out at two locations, and for mancozeb, persistence studies were carried out at four locations. Initial deposits of mancozeb on apple fruits ranged from 1.33 to 1.63mg/kg at the recommended dose and from 2.55 to 3.26mg/kg at double the recommended dose at all four locations. Chlorpyrifos residues in apple fruits had an initial deposit of 0.94-0.99mg/kg at recommended dose and 1.75-1.92mg/kg at double the recommended dose. Mancozeb residues in apple fruit were below the detection limit (BDL) after 20days at recommended dose and after 25days at double the recommended dose at two locations, while mancozeb residue at the other two locations and the residues of chlorpyrifos at all locations reached BDL after 15 and 20days at recommended and double the recommended doses, respectively. Half-life of mancozeb varied from 3.07 to 4.02days at recommended dose and from 3.30 to 4.32days at double the recommended dose, whereas chlorpyrifos residues dissipated to half their initial concentration on 2.33-2.35days at recommended dose and 2.89-2.90days at double the recommended dose. The soil samples showed no presence of residues of chlorpyrifos and mancozeb at harvest. The risk assessment revealed that hazard quotient for the intake of mancozeb was in the range of 0.06-0.13% and 0.20-0.44% for rural and urban population, while for the intake of chlorpyrifos, hazard quotient was in the range of 0.10-0.12% for rural population and 0.33-0.38% for urban population, and theoretical maximum dietary intake (9.67 × 10-5mg/person and 3.18 × 10-4mg/person for rural population and urban population in case of mancozeb and 3.22 × 10-5mg/person and 1.06 × 10-4mg/person for rural population and urban population in case of chlorpyrifos) was also found to be less than maximum permissible intake (1.38mg/kg for mancozeb and 0.60mg/kg for chlorpyrifos). The results of risk assessment thereby indicated that apple consumption does not pose a risk to human health.

Residue characteristics of seven fungicides in cherry tomatoes and vegetable tomatoes

AbstractA modified QuEChERS method using a GC-ECD to determine the multiple residues of pyraclostrobin, difenoconazole, dimethomorph and azoxystrobin and to indirectly determine the total residues of maneb, mancozeb and propineb by a GC-FPD (with an S filter) was established and validated. Meanwhile, field trials were conducted in accordance with good agricultural practice (GAP) to study their characteristics of residue degradation under the agricultural climate and cropping system of Guangxi Province. The separation effect of each target peak was good with a linearity range of 0.01–5 mg L−1, a limit of detection (LOD) of 0.003–0.015 mg kg−1 and a limit of quantification (LOQ) of 0.01–0.05 mg kg−1. The average recovery ranges of vegetable tomatoes and cherry tomatoes were 70.5–120.0% and 70.8–119.8%, respectively, with relative standard deviations (RSDs) of less than 7.1%. Field trials of seven fungicides in vegetable and cherry tomatoes showed that the half-lives (t1/2) of the dithiocarbamate fungicides (metiram, mancozeb, and propineb, defined as total residues determined as CS2), pyraclostrobin, difenoconazole, dimethomorph, and azoxystrobin were in the ranges of 5.2, 12.7–17.8, 7.6–7.9, 6.6–6.9, and 6.3–6.6 d in vegetable tomatoes, respectively. The cherry tomatoes presented ranges of 4.3–4.5, 10.8–11.8, 6.7–7.0, 5.4–5.5, and 5.9–6.2 d, respectively. Combined with the final residue and market monitoring results, the results show that cherry tomatoes have significantly higher terminal residues, initial deposits, and maximum residues of seven fungicides than vegetable tomatoes, and these seven pesticides can be detected in cherry tomatoes purchased from three markets. Therefore, cherry tomatoes may be regarded as representative varieties of tomatoes in realizing residual extrapolation for the establishment of the maximum residue limit (MRL) value of fungicides in tomatoes and for conducting market monitoring.

Method optimization and validation for the determination of mancozeb in chamomile by modified QuEChERS and liquid chromatography–tandem mass spectrometry

Herein we present the optimization and validation of a simple, rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC–MS/MS) method for the determination of mancozeb residue in chamomile for the first time to our knowledge. The sample preparation procedure was based on the decomposition of mancozeb in an alkaline medium followed by derivatization with dimethyl sulfate to form dimethyl ethylenebisdithiocarbamate (EBDCS-dimethyl). In order to improve the recovery and separation of EBDCS-dimethyl from chamomile matrix the chromatographic condition, sample pretreatment, and clean-up were optimized. An optimized mobile phase consisting of methanol– ammonium formate buffer at 50 %:50 % in the isocratic program resulted in the separation of EBDCS-dimethyl successfully without any interference. Additionally, the highest recovery (88 %) was obtained when acetonitrile was used as an extraction solvent followed by acetonitrile 0.1 % F.A and ethyl acetate. Optimal methylation was obtained upon shaking the sample for 15 min and using dimethyl sulfate at 0.05 M. Moreover, it was found that a sorbent combination of 150 mg/mL MgSO4 + 25 mg/mL PSA was optimal for decreasing the sample interferences while maintaining a high recovery (87 %). The mean recoveries of two spiking levels (0.05 and 0.1 mg/kg) were 86.4 % and 81.9 %, with a relative standard deviation of 12.1 and 11.8 respectively. Furthermore, the limits of quantification reached by the method equaled 0.05 mg/kg which is satisfying the maximum residue level (MRL) set by the European Union for mancozeb in chamomile.

Electrochemical quantification of mancozeb through tungsten oxide/reduced graphene oxide nanocomposite: A potential method for environmental remediation

The extensive use of pesticides for better yield of crops have become major human concern over the decades. Pesticides are widely used in the fields to kill weeds and pests on the vegetable and crops to improve the quality and yield of the food knowing the fact that pesticides residue in food are very lethal for human being. Amongst, the hazardous pesticides, mancozeb is widely applied in the protection of crops. Thus the quantification of mancozeb residue is of great importance. This study reports the electrochemical monitoring of mancozeb through tungsten oxide reduced graphene oxide (WO3/rGO) nanocomposite. The engineered nanocomposite was characterized though different analytical tools such as FTIR, XRD and TEM to examine crystallinity, internal texture and the size. The FTIR result confirm the functionalities of GO and WO3/rGO nanocomposite in finger print and functional group region. Through XRD analysis, the size of the WO3/rGO nanocomposite was calculated as 31.6 nm. While the TEM analysis was also exploited to examine the 2D texture of GO and nanometric size of the WO3/rGO. To ensure the conductive nature of the WO3/rGO nanocomposite, the glassy carbon electrode was modified and exploited for cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the modified sensor showed exceptional response for mancozeb. The linear dynamic range was set from 0.05 to 70 μM in BRB buffer of pH 4. The LOD and LOQ for proposed method was calculated as 0.0038 and 0.0115 μM. The analytical applicability of chemically modified sensor was investigated in real matrix of different vegetable samples and the recovery values were observed in acceptable range. The electrochemical examination of present work reveals that WO3/rGO nanocomposite can be an exceptional aspirant for the determination of mancozeb at commercial level.

Dissipation of carbendazim and mancozeb following application of combination product on soybean and cotton

Dissipation and harvest time residues of carbendazim and mancozeb were studied in cotton and soybean following application of combination product (carbendazim 12% + mancozeb 63%). The residues of carbendazim and mancozeb persisited till 30th (harvest) and 50th (harvest) day in cotton leaf sample. Half-life of carbendazim in soybean was 13.1 and 16.7 days and in cotton was14.3 and 15.8 days, respectively, at recommended and double dose. The half-life of mancozeb at recommended and double dose was 3.5 and 4.1 days and 4.1 and 4.7 days, respectively, in soybean and cotton.The harvest time residues of carbendazim and mancozeb were below LOQ (< 0.05 mg kg−1) in all the soybean and cotton samples. The residues persisted till 30th day with levels of 0.3 and 0.5 mg kg−1 at different doses in the soybean pod samples from Anand location. A preharvest interval of 30 and 50 days is proposed in soybean and cotton, respectively.

Assessment of the Pesticides Utilization and the Pesticide Residues Presence in Fresh and Tomato Products for the Tomato Supply Chain in Rwanda

Pesticide residues are either natural or synthetic and are found in most edible products such as fresh fruits, vegetables, meat and other processed products. Excess of these pesticides’ residues is unsafe for consumption due to regulatory measures. Thus, it is beneficial to assess the level of residues of pesticides in the food diet in order to improve the standard of living of the population. This study intends to assess the pesticide usage and residue levels of selected pesticides in fresh tomatoes in the supply chain of Rwanda. The results of the study confirm that there are around 10 brands of pesticides used in Rwanda. 58.97% of the surveyed people around the country are males and 71.79% of farmers apply rockets as the main pesticide. More than 22% of surveyed farmers affirmed to consume the raw or/and unwashed tomatoes. This study revealed that the residues of the active ingredients contained in the pesticides are accumulated in unwashed tomatoes and the quantity of such residues is shown in this study. Deltamethrin was 0.64 ± 0. 009 mg·kg?1 in tomatoes collected in Rusizi district, cypermethrin 1.1435 ± 0. 0375 mg·kg?1 in tomatoes collected from Gisagara District, mancozeb residues was 0.620 ± 0.000 mg·kg?1 in tomatoes collected from Rusizi district. The results of the study also show that pesticide residues are not found in the washed tomatoes. Therefore, the detection of pesticide residues in unwashed tomatoes in some samples indicates the malpractice of residues among farmers in Rwanda. Also, consumers eating the unwashed fresh tomatoes are recommended to wash the tomatoes before use.

Optimization of straightforward methods for the monitoring of organic and inorganic contaminants in strawberries

Straightforward methods for the monitoring of chlorothalonil, cyprodinil, fludioxonil, metalaxyl-m, mancozeb and ethylenethiourea in strawberries, based on modified QuEChERS protocols followed by HPLC-DAD, were validated according to DG-SANTE Guidelines. Compounds showed satisfactory recoveries in the range 85.4–100.3% at spiking levels of 0.01, 0.02 and 0.05 mg kg−1, with RSDs below 15 %. LOQs were in the range 0.01 – 0.02 mg kg−1, being below the corresponding maximum residue levels (MRLs). Also, a simple method for the determination of Cd and Pb, based on a microwave-assisted digestion followed by electrothermal atomic absorption spectrometry (ETAAS), was validated according to Eurachem Guide. Trueness and precision were evaluated by analysis of a certified reference material (CRM). Results were statistically equivalent to the certified values at the 95 % significance level, while RSDs were below 5%. Methods were finally applied to the analysis of commercial samples. Residues of mancozeb and ethylenethiourea were detected in some of the samples but below the LOQs. Inorganic contaminants were in the range 0.0031 – 0.0088 mg kg−1 (Cd) and 0.0057 – 0.0212 mg kg−1 (Pb) (fresh weight), being below the corresponding maximum limits established by national regulation. The proposed methods can be easily implemented as routine analysis for food safety monitoring.

Modification of the existing maximum residue levels for mancozeb in various crops.

In accordance with Article 6 of Regulation (EC) No 396/2005, the applicant Indofil Industries Limited submitted a request to the competent national authority in Germany to modify the existing maximum residue levels (MRLs) for the active substance mancozeb in garlic, broccoli, cauliflowers and leeks. The data submitted in support of the request were found to be sufficient to derive MRL proposals for the crops under consideration. Based on the risk assessment results, EFSA derived recommendations for further consideration by the risk managers. Adequate analytical methods for enforcement are available to control the residues of mancozeb in the crops under consideration.

Feasibility of Vis/NIR spectroscopy to detect and estimate fungicide residues on intact lettuces

Pesticides are applied repeatedly to grain, fruit, and vegetable crops for protection against pathogens, pests, and weeds, in short periods of time before harvest. The effective and fast monitoring of chemical residues in agricultural products is important for the assurance of healthy food. This study was accomplished to evaluate the feasibility to detect and estimate the concentration of dithiocarbamate fungicide (mancozeb) residues on intact lettuce leaves based on Vis/NIR spectral reflectance measurements and multivariate data analysis. In the pre-harvest interval, a high initial rate of decline of dithiocarbamate residues was observed between one and seven days after pesticide spraying (decrease of 90.3%), while a slower decline was verified from seventh to fourteenth day (decrease of 8.7%). The usefulness of this spectrometric method has been evidenced by determination of dithiocarbamate residues at concentrations between 0.23 and 10.3 mg CS2 kg-1, with detection and quantitation limits of 0.49 and 1.41 mg CS2 kg-1, respectively. Vis/NIR spectral reflectance combined to the partial least square analysis have potential to be applied for estimating dithiocarbamate concentrations on intact lettuce leaves, presenting advantages such as real-time measurements and the possibility to be built into the industrial processing lines.

Residues dnd dissipation of mancozeb 75% WP in/on onion

Supervised field trial was conducted to determine the dissipation of mancozeb (based on CS2) in/on onion and in cropped soil resulting from spray application of mancozeb 75 WP at recommended dose (1500 g a.i./ha) and double the recommended dose (3000 g a.i./ha). The initial residues of mancozeb (based on CS2) in immature onion including leaves were found to be 3.44 mg/kg and 5.89 mg/kg at recommended dose and double the recommended dose, respectively. Mancozeb residues (based on CS2) dissipated below limit of quantification (LOQ) on 10th and 15th day with half-life values of 1.91 and 2.33 days at recommended dose and double the recommended dose, respectively. The residues of mancozeb (based on CS2) in mature onions and soil collected at harvest were found to be below limit of quantification (LOQ).

Determination of Dithiocarbamate Mancozeb Residues in Milk Samples Using GC-MS Method

The present study was performed to validate gas chromatography mass spectrometry analytical method for the determination of mancozeb residues in milk samples. The analytical method is based on the extraction procedure by using mineral acids. The extraction solution was sucked into the flask, the dripping funnel was quickly exchanged for the gas inlet. After 2 hours, the adsorption tube containing the isooctane was removed and closed. The solution was transferred into a sample vial and analysed using gas chromatography- mass spectroscopy (GC-MS). A Rxi-624Sil MS (30 m length x 0.32mm I.D. x 1.8 μm particle size capillary column is used for the separation. The method has linearity over the range 0.05 to 10.0 μg/mL. Recovery study was conducted at 0.05 and 0.5 mg/kg fortification levels. The average mean recoveries were calculated as 86.23 % at 0.05 mg/kg level and 92.69 % at 0.5 mg/kg level. The limit of quantification (LOQ) in milk was established as 0.05 mg/kg.

Rainfastness of mancozeb on apple seedlings determined through deposition quantification of mancozeb residue and a fluorescent pigment

Mancozeb spray deposition and the persistence thereof to rainfall are important factors influencing the efficacy of apple scab control caused by Venturia inaequalis. Fungicide deposition can be assessed through quantification of fungicide residue, or fluorescent pigment quantification. Fluorescent pigment quantification is a more cost effective and less labour intensive method, since it is assessed using only photomacrography and image analyses. The yellow fluorescent pigment used in this study was shown to be a suitable tracer for five different mancozeb formulations on apple leaves when evaluated at different concentrations (0.5, 1.0, 1.5 and 2.0). Pearson's correlation was significant (P < 0.001) and high (r = 0.896) between fluorescent particle coverage (FPC%) and mancozeb residue (mg/kgDry Weight) for all five formulations. The particle size ranges of two wettable powder (WP) formulations were significantly smaller than those of the other WP formulations, but this did not result in differences in mancozeb residue on apple leaves. The persistence of mancozeb to different rain volumes applied to apple leaves was determined for three mancozeb formulation treatments: Dithane M-45 800 WP NT, Ventum 800 WP, and Ventum 800 WP combined with the sticker-spreader adjuvant Nu-Film P. There were no significant differences (P > 0.495) between the three treatments based on FPC% and mancozeb residue quantity when simulated rain was applied to apple seedlings at a constant rainfall intensity of 5 mm/h at five different rainfall volumes (0, 1, 5, 10 and 15 mm). A fair to good correlation (r = 0.697 and 0.995) existed between FPC% and mancozeb residue, and their percentage loss at different rainfall volumes. However, the response of FPC% and mancozeb residue differed based on exponential regression curves. This was due to a markedly larger predicted loss by each model's asymptote, 51.67% for FPC% and 40.76% for mancozeb residue. Based on actual mancozeb residue values, a significant percentage loss in residue already occurred after applying 1 mm (32.90%) rain. When rain volumes were increased to 5 and 10 mm rain, the percentage losses (37.88 and 41.08%) did not differ significantly from 1 mm rain. The same was true for percentage loss in FPC%, except that a significant higher loss occurred at 10 mm (52.36%) than at 1 mm rain (41.13%).

Determination of mancozeb residue in fruit by derivatization and a modified QuEChERS method using ultraperformance liquid chromatography-tandem mass spectrometry.

A liquid chromatography-tandem mass spectrometry method with derivatization and a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation was developed for the determination of mancozeb in fruits. The target compound was determined in less than 4.0min with use of an electrospray ionization source in positive mode. The limits of detection and the limits of quantification ranged from 1.0 to 3.2μgkg-1 and from 10 to 15μgkg-1 in fruit respectively. The linearity was excellent for mancozeb (R 2 ≥ 0.9920). Recoveries in five matrices were obtained at three spiking levels (0.02, 0.1, and 1mgkg-1). For all concentrations, the mean recoveries ranged from 84.0% to 95.9%, with repeatability relative standard deviation (n = 5) of 0.6 - 7.0%. The interday reproducibility relative standard deviation (n = 3) ranged from 1.4% to 5.5%. This method could be used for the routine detection of mancozeb residues in fruit. Graphical abstract The derivation of mancozeb and the detection process of derivative product by UPLC-MS/MS.

Dissipation Behavior of a Mancozeb Residue (Dithiocarbamate Fungicide) in Tomato Under South Moroccan Climatic Condition

&lt;div&gt;&lt;p&gt;&lt;em&gt;Dissipation behavior of mancozeb, a widely used dithiocarbamte fungicide, applied under field condition on tomato at recommended dose was undertaken under greenhouses and open field during October and March in Souss-Massa area (south of Morocco) climatic condition. Residue levels of mancozeb were determined by using a simple method consisting in a hot acid digestion of the whole sample to evolve carbon disulfide (CS&lt;sub&gt;2&lt;/sub&gt;), which is further quantified by spectrophotometry. Degradation rate in both open field and greenhouse followed first order kinetic. Half lives were 1.77 and 1.3 days in open field and in greenhouse were 2.0 and 1.8 days for October and March period respectively. Based on the observation reported, a pre harvested interval of at least three days after pesticide application at recommended dose may be suggested.&lt;/em&gt;&lt;/p&gt;&lt;/div&gt;

Mancozeb Residue on Tomatoes in Central Uganda.

Mancozeb belongs to a group of pesticides known as dithiocarbamates (DTC) that are a non-systemic group of pesticides extensively used in Uganda to protect crops from fungal diseases. This study was done in 5 selected districts of Central Uganda with a focus on markets and farms to investigate the current mancozeb concentrations on tomatoes and identify key areas of improvement to minimize human exposure. Tomato samples were analyzed for mancozeb residue determined as carbon disulfide (CS2) by gas chromatography-mass spectrometer (GC-MS). All the samples analyzed had detectable concentrations of mancozeb residue. It was observed that farm samples had mean concentrations of 1.03±0.28 mg/kg, while market samples had 0.77±0.49 mg/kg. The study also found that farmers applied 3-6 times the dosage of mancozeb recommended by manufacturers. Furthermore, the observed pre-harvest interval after application of mancozeb was 1-2 days as opposed to 3-7 days set by manufacturers. The observed practices at farms are likely to put farmers and final consumers at a risk of exposure to dithiocarbamates.

Mancozeb Residue on Tomatoes in Central Uganda

Mancozeb Residue on Tomatoes in Central Uganda

Degradation pattern and risk assessment of carbendazim and mancozeb in mango fruits.

A supervised field trial was conducted at four different agroclimatic locations in India to evaluate the dissipation pattern and risk assessment of carbendazim and mancozeb in mango fruits following foliar application of mixed formulation of carbendazim 12% and mancozeb 63% fungicide (SAAF-75WP) at recommended dose (90 + 472.5) and double the recommended dose (180 + 945 g a.i. ha(-1)). Average initial deposition of carbendazim was in the range of 1.12 to 2.7 and 1.95 to 4.09 mg kg(-1) and for mancozeb in the range of 2.25 to 2.71 and 4.17 to 5.96 mg kg(-1), given at respective doses. Residues of carbendazim and mancozeb were dissipated to the below detectable limit 7 days after spray at recommended dosage in all the locations. The fungicide degradation followed a first order kinetics with half-lives of 1-5 and 1-3 days, for carbendazim and mancozeb, respectively. The TMRC values, calculated from residue data generated from all four locations, were found to be below the MPI in mango fruit, and hence, the fungicide will not cause any adverse effect after consumption of mango fruits. This data could provide guidance for the proper and safe use of this fungicide mixture for managing disease incidence in mango in India.