Residual Soil Research Articles

Effect of Biological Products on Yield, Production Economics and Soil Nutrient Status of Transplanted kharif Rice (Oryza sativa L.) in Gangetic Alluvial Soil of West Bengal, India

Field experiment was conducted during kharif season of 2017 and 2018 at Instructional Farm under Bidhan Chandra Krishi Viswavidyalaya, West Bengal in sandy loam soil to study the growth and yield of transplanted kharif rice variety Satabdi (cv. IET 4786) as influenced by biological products. The experiment was laid down in randomized complete block design (RCBD) with seven treatment combinations replicated thrice. Results revealed that RDF + soil applied Bolt GR @ 10 kg/ha produced higher growth attributes and yield of tested rice cultivar. The same treatment registered significantly higher total N and K uptake in tested cultivar; while the highest P uptake was recorded with RDF + Seed treatment with JumpStart2.0 @ 0.83 ml/kg seed. The treatment RDF + soil applied Azospirillum @ 2 kg/ha brought about significant positive changes of available N content in post-harvest soil over control situation (only RDF). However, significantly higher available P content was estimated in plots with RDF + soil applied PSB @ 2 kg/ha. Application of bio-products failed to exert any significant influence on residual soil K. The crop receiving RDF + soil applied Bolt GR @ 10 kg/ha gave highest gross return, net return and B:C ratio. Hence, application of RDF (60-30-30 kg N, P2O5 and K2O/ha) along with Bolt GR @ 10 kg/ha or Azospirillum @ 2 kg/ha may be recommended to achieve higher grain yield of tested cultivar Satabdi (cv. IET 4786).

Paclobutrazol persistence dynamics in mango (Mangifera indica) orchard soil using HPLC and LC-MS/MS analysis

Paclobutrazol, a plant growth regulator, is used commercially in soil application to counter the alternate bearing habit of mango (Mangifera indica L.) all over the country. The experiments were conducted during 2020–21 and 2021–22 at ICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh to study the persistence of paclobutrazol in soil and its subsequent uptake in mango fruits using HPLC and validated by LC-MS/MS after its application to tree basin soil of mango cv. Dasheri @2, 4, 6, 8 and 10 g a.i./tree. The residues of paclobutrazol dissipated in soil from its initial values of 10.33, 17.25, 25.71, 50.80, and 62.36 µg/g soil at 0 days to 0.14, 0.30, 0.47, 1.32 and 2.38 µg/g soil after 300 days of application. The dissipation rate in soil followed first-order kinetics with half-life values ranging between 57.75 and 77.00 days. Residues of paclobutrazol were not detected in fully mature fruits harvested after 85 days of fruit set. LC-MS/MS analysis confirmed that the sample peaks corresponded to paclobutrazol residues in mango fruit and soil. Thus, HPLC and LC-MS/MS analysis clearly showed that the paclobutrazol persisted in the soil for longer, but the mature fruits harvested from treated trees were free from its residue even at higher doses and safe for consumption. The response of paclobutrazol to the quality of fruits in terms of phenolic content was also assessed. The paclobutrazol was more responsive in altering phenolic acid composition than the control.

Characterization and source identification of PM2.5 during intense haze episodes in an urban environment of Lahore

In the backdrop of persistent haze occurrences affecting Southeast Asia and Pakistan's environmental landscape, this study delves into an in-depth analysis of atmospheric Particulate Matter (PM2.5) during intense haze episodes prevalent in Lahore throughout October, November, and December 2019. Employing advanced analytical techniques encompassing Scanning Electron Microscopy (SEM) coupled with Energy-Dispersive Spectroscopy (EDX), X-ray Diffraction (XRD), and Raman Spectroscopy (RS), this investigation meticulously scrutinized PM2.5 samples. The findings showcased substantial variability in PM2.5 concentrations, peaking notably in December within the range of 43.2–301 μgm−3, averaging 168 ± 88.3 μgm−3, whereas lower concentrations ranging from 30.9 to 268 μgm−3, with an average of 106 ± 66.1 μgm−3, were observed in October. These concentrations displayed correlations with meteorological parameters, demonstrating a direct association with relative humidity and varying relationships with temperature and wind speed. The maximal PM2.5 concentrations aligned with lower temperatures (19.1 °C), while higher temperatures (26.1 °C) coincided with the lowest concentrations, illustrating distinct relationships with relative humidity percentages and wind speeds. Advanced spectroscopic analyses (RS and XRD) confirmed the presence of various minerals and elements within PM2.5 samples, encompassing calcite, calcium aluminosilicate, hematite, barite, quartz, gypsum, organic carbon, and nineteen elements identified by EDX. Morphological evaluations unveiled diverse particle shapes, from round, pointed, and irregular to rod-like, and agglomerate structures. SEM investigations delineated distinctive groups of anthropogenic and geogenic particles, emphasizing emission sources such as automobile emissions, crop residue burning, biomass burning, construction activities, soil dust, and industrial emissions. This comprehensive study lays the groundwork for source apportionment, vital for understanding consequential impacts on climate, visibility, and human health, fostering future investigations in this domain.

Impact of compost and biochar from agricultural waste on reducing cadmium concentration and mancozeb residue in soil

The negative impact of excessive exposure to agrochemicals in shallot cultivation causes environmental pollution and human health. Biochar has the potential to absorb agrochemical contamination. This research aimed to investigate the effect of providing compost and biochar from agricultural waste on land quality, reducing the concentration of heavy metal cadmium (Cd) and mancozeb pesticide residues in soil and products in shallot. The experiment was carried out in shallot fields in Ngurensiti Village, Pati Regency, Central Java Province. Four different treatments, including combinations of biochar and compost, were applied, along with conventional controls. Data were analyzed using the F test (ANOVA) and Tukey's test using the Minitab statistical program version 16.0. The research showed that using biochar made from sugarcane bagasse, rice husk, corncob, and compost helped more soil bacteria grow and lowered Cd and mancozeb concentrations. In addition, treatment with biochar from sugarcane bagasse waste showed a decrease in Cd and mancozeb concentrations and a more significant increase in bacterial populations compared to other treatments (rice husk biochar and corncob biochar). Although there was a slight increase in Cd concentration in shallot leaves post-treatment, Cd levels in shallot bulbs remained within safe limits. This study shows that using biochar and compost from agricultural waste effectively improves soil quality, reduces heavy metal pollution, and lowers pesticide levels to support sustainable agriculture and protect people's health.

Strength behaviour of soil blended with two waste materials-fly ash and tire crumbs with cement

Abstract The rapid development in the economy has led to a growing need for electricity and automobiles, leading to the production of large quantities of fly ash (FA) and discarded tires. The safe disposal of these materials has become a significant problem. FA and scrap tyre derived material has some useful properties which can be beneficially used for enhancing the engineering properties of soil. Therefore, the study investigates the combined impact of FA and scrap tire-derived material on the stress-strain-strength behaviour of a fine-grained residual lateritic soil, incorporating a cementing agent. The research involves compaction tests followed by triaxial compression tests, where FA content added at an increment of 15% starting from 20% by weight of soil. The maximum FA content used in the mix is 50% by weight of soil. Tyre crumb (TC) which is a scrap tire-derived material, is also included in the study, with TC content ranging from 5% to 10% by weight. Additionally, 2% by weight of cement is added to each soil mix as a binding agent. Specimens are compacted in accordance with specific compaction parameters and subsequently cured for duration of up to 28 days. Strength tests are then carried out on those specimens to analyse their strength behaviour. This research primarily examines the shear strength characteristics of soil blended with FA, cement, and TC, emphasizing their geotechnical performance. Addition of 5% TC increases the peak deviator stress of cemented mix having 50% FA content from 1351 kPa to 2710 kPa at 300 kPa confining pressure and 28 days curing period. The inclusion of TC to soil-FA-cement blends is noted to significantly enhance shear strength when compared to mixes without tire crumb. Also, the stress-strain behaviour of soil is significantly influenced by addition of FA and cement in the presence of TC. Therefore, there is an ample scope of utilization of soil-fly ash-cement-TC mixes in geotechnical applications.

Impact of Rice Husk Ash Based-Geopolymer on Some Geotechnical Properties of Selected Residual Tropical Soils

Impact of Rice Husk Ash Based-Geopolymer on Some Geotechnical Properties of Selected Residual Tropical Soils

In Silico Determination of The Antifungal Effect of Plant Active Molecules Against Botrytis Cinerea by Molecular Docking

Botrytis cinerea, which has developed many strategies to infect plants, can survive in harsh environmental conditions, and has a wide host range, has become an important problem both economically and ecologically by causing tons of crop losses for many years. The residues in soil and crops caused by chemical pesticides used to get rid of agricultural pests pose serious threats to human and environmental health, such as hormonal abnormalities and acute respiratory poisoning, especially in children. The most critical step to avoid these hazards will be to replace chemical pesticides with plant-active molecules. At the same time, these studies primarily in silico will provide a return in terms of both time and cost. Inhibition of pectin methyl esterase, an important virulence factor of B. cinerea, will ensure the organism is controlled. In order to determine candidate biofungicide effector molecules, QSAR parameter values of 409 plant active molecules were calculated. Firstly, conformer distribution and geometry optimizations were performed with Spartan 14’ software. Docking studies of the optimized molecules were carried out through Autodock Vina software, while visualization studies to make sense of the interactions between the target receptor structure and effector molecules were used by BIOVIA Discovery Studio software. As a result of all the analyses, the molecules that are alternatives to chemical pesticides as biofungicides were determined to be the following molecules: Podolactone B, Repin, Sandaracopimaradienediol, 6-Hydrogenistein, Artemisinin, Lycoricidine, 6-Methoxygossypol, Viscidulin, Ciprofloxacin, and 7,4’-Dihydroxyflavan.

Preliminary Experiences in Determining the Soil–Water Characteristic Curve of a Sandy Soil Using Physical Slope Modeling

Relating soil moisture content to soil suction, the soil–water characteristic curve (SWCC) represents an essential feature in unsaturated soil mechanics that enables estimation of different unsaturated soil property functions and modeling of the macro-scale soil behavior. However, depending on the soil and processes under consideration, proper hydraulic characterization of a soil through direct laboratory measurements can be difficult, time-consuming, and involve many uncertainties. In the case of uniformly graded sands, there is a highly nonlinear and steep shape of the SWCC, with only a few kPa of soil suction separating saturated and residual soil moisture conditions, which makes measurements for determinations of SWCC especially challenging. This study encompasses an investigation of the sandy type of soil’s behavior and presents some preliminary results and experiences on the determination of SWCC through the use of physical slope model tests. The 30 cm deep slope, inclined at 35 degrees and instrumented with soil moisture and pore water pressure sensors, was exposed to series of rainfall intensities, ranging from 37 up to 300 mm/h. The results indicated that the data on hydraulic response in monitored points are not only useful for the determination of SWCC, but that the approach is useful for investigation of hydraulic hysteresis phenomena, as well as its effects on soil moisture and pore water pressure conditions, which also affects the stability conditions of a slope. In particular, the best-fit parameters of the van Genuchten model suggested air entry values of 1.6 and 1.1 kPa for the drying and the wetting curves of the SWCC, respectively, with the two branches shifted by about 1 kPa of soil suction.

Experimental Study and Analysis of the Effects of Mud on the Compressive Strength of Unburned Brick Using Engineering Residue Soil

Experimental Study and Analysis of the Effects of Mud on the Compressive Strength of Unburned Brick Using Engineering Residue Soil

Mesoporous architectural magnetic halloysite-polymer beads for removing toxic streptomycin from water: A sustainable remediation approach

Streptomycin (STR) is a widely used antibiotic to treat various infectious diseases in humans and animals. Increased STR production and distribution result in harmful residue in soil and water. Consequently, STR exists in biotic- and abiotic-counterpart of the environment and poses potential toxicity and risk due to its bioaccumulation and biomagnification properties. Sustainable remediation of STR from wastewater requires selective, minimal, low-cost, regenerable, and reusable materials as adsorbents. In this study, magnetic-halloysite incorporated polymer composite beads (SPHM) were synthesized and used for the efficient clean-up of toxic STR from wastewater. SPHM has a mesoporous structure with an abundance of oxygen-containing functional groups and exhibits a synergistic STR clean up performance (qm = 235.71 ± 13.98 mg/g). Sorption and interfacial studies revealed that diffusion, hydrophobic and ionic interactions, including electrostatic interaction, are involved in STR remediation. Electrostatic interaction plays a vital role alongside the physical sorption mechanism due to the presence of hydroxyl and carboxyl groups induced from poly (vinyl alcohol) and sodium alginate. Moreover, X-ray photoelectron spectroscopy (XPS) and Time-of-flight secondary ion mass spectrometry (ToF-SIMS) analyses confirm the involvement of opposing charged groups of SPHM and STR in adsorption. SPHM can be magnetically separated in just 20 s and is regenerable and reusable up to 10 times, with outstanding performance and stability. The sorption process requires only a minimal amount of SPHM, i.e., 0.5 g/L for STR clean-up. Even the natural surface water composition did not affect its performance. Hence, natural nanoclay-based, biocompatible and low-cost SPHM has a great potential for the sustainable remediation of streptomycin and other similar antibiotics from wastewater.

Simulating Two-Phase Seepage in Undisturbed Soil Based on Lattice Boltzmann Method and X-ray Computed Tomography Images.

The two-phase seepage fluid (i.e., air and water) behaviors in undisturbed granite residual soil (U-GRS) have not been comprehensively studied due to a lack of accurate and representative models of its internal pore structure. By leveraging X-ray computed tomography (CT) along with the lattice Boltzmann method (LBM) enhanced by the Shan-Chen model, this study simulates the impact of internal pore characteristics of U-GRS on the water-gas two-phase seepage flow behaviors. Our findings reveal that the fluid demonstrates a preference for larger and straighter channels for seepage, and as seepage progresses, the volume fraction of the water/gas phases exhibits an initial increase/decrease trend, eventually stabilizing. The results show the dependence of two-phase seepage velocity on porosity, while the local seepage velocity is influenced by the distribution and complexity of the pore structure. This emphasizes the need to consider pore distribution and connectivity when studying two-phase flow in undisturbed soil. It is observed that the residual gas phase persists within the pore space, primarily localized at the pore margins and dead spaces. Furthermore, the study identifies that hydrophobic walls repel adjacent fluids, thereby accelerating fluid movement, whereas hydrophilic walls attract fluids, inducing a viscous effect that decelerates fluid flow. Consequently, the two-phase flow rate is found to increase with then-enhanced hydrophobicity. The apex of the water-phase volume fraction is observed under hydrophobic wall conditions, reaching up to 96.40%, with the residual gas-phase constituting 3.60%. The hydrophilic wall retains more residual gas-phase volume fraction than the neutral wall, followed by the hydrophobic wall. Conclusively, the investigations using X-ray CT and LBM demonstrate that the pore structure characteristics and the wettability of the pore walls significantly influence the two-phase seepage process.

Study on the Conveying Characteristics of a Hanged Harvester Vibrating Screen for Ligusticum Chuanxiong

The problem of soil and Chuanxiong tuber congestion on vibrating screens usually exists during the Chuanxiong mechanized harvesting process. To address this problem, the conveyance performance of a crankshaft rocker vibrating screen was studied. By establishing and solving the dynamics and kinematics equations for the crankshaft rocker vibrating mechanism and Chuanxiong soil residue, the acceleration of the vibrating screen and Chuanxiong-soil residue was studied. The sliding speed, motion process, and conveying distance of the Chuanxiong soil residue were also analyzed. The theoretical analysis results indicated that the acceleration of the vibrating screen depends on the rod lengths of the vibrating mechanism and the crank rotational speed and position. The displacement of the Chuanxiong-soil residue along the positive sliding direction in a cycle was more significant than that of the negative sliding direction. The appropriate advancement speed of the harvester was also obtained. The RecurDyn and EDEM coupling simulation was conducted. The simulation results verified the theoretical analysis. In the simulation, the Chuanxiong-soil residue was effectively conveyed. The field tests were conducted to verify the theoretical analysis. The harvester was tested in the field with crank rotational speeds of 0 r/min, 120 r/min, and 240 r/min, and advancement speeds of 0.5 m/s, 1 m/s, 1.5 m/s, and 2 m/s. The results showed that there was no congestion in the screen during the working process when the rotational speed of the crank was 240 r/min, and the advancement speed of the harvester was no faster than 1.5 m/s. When the crank rotational speed was 240 r/min, and the advancement speeds were 0.5 m/s, 1 m/s, and 1.5 m/s, the weights of Chuanxiong and soil on the screen after the test were 71.5 kg, 84.7 kg, and 105.7 kg, respectively. The field tests verified the conveyance performance of the vibrating screen. This study can provide a theoretical reference for designing the crankshaft rocker vibrating mechanism for the rhizome harvesting machine.

Pesticide residues in boreal arable soils: Countrywide study of occurrence and risks

Large volumes of pesticides are applied every year to support agricultural production. The intensive use of pesticides affects soil quality and health, but soil surveys on pesticide residues are scarce, especially for northern Europe. We investigated the occurrence of 198 pesticide residues, including both banned and currently used substances in 148 field sites in Finland. Results highlight that pesticide residues are common in the agricultural soils of Finland. A least one residue was found in 82% of the soils, and of those 32% contained five or more residues. Maximum total residue concentration among the conventionally farmed soils was 3043 μg/kg, of which AMPA and glyphosate contributed the most. Pesticide residues were also found from organically farmed soils, although at 75–90% lower concentrations than in the conventionally farmed fields. Thus, despite the application rates of pesticides in Finland being generally much lower than in most parts of central and southern Europe, the total residue concentrations in the soils occurred at similar or at higher levels. We also established that AMPA and glyphosate residues in soil are significantly higher in fields with cereal dominated rotations than in grass dominated or cereal–grass rotations. However, risk analyses for individual substances indicated low ecological risk for most of the fields. Furthermore, the total ecological risk associated with the mixtures of residues was mostly low except for 21% of cereal dominated fields with medium risk. The results showed that the presence of mixtures of pesticide residues in soils is a rule rather than an exception also in boreal soils. In highly chemicalized modern agriculture, the follow-up of the residues of currently used pesticides in national and international soil monitoring programs is imperative to maintain soil quality and support sustainable environment policies.

Biodegradation rate and build-up of plastics in soil: A theoretical approach

The ecological risk assessment of a product released into the environment is a complex process that takes into account both its ecotoxicity and the Predicted Environmental Concentration (PEC) in the environment. The latter depends on the use, transport, fate (i.e. persistence) of the product. This article describes a model to determine the Predicted Environmental Concentration (PEC) of a biodegradable mulch film taking into account its characteristics, the frequency of application of the mulch film, its degradation rate, and the density of the soil to which it is applied. The effect of temperature on biodegradation kinetics was also taken into account to estimate the biodegradation rate achievable at a given temperature based on data obtained in the laboratory under standard conditions. Using the same approach as for pharmacokinetics, the model can calculate the average mulch film concentration at steady state and the maximum concentration applied. The PEC values can be compared with the Predicted No-Effect Concentration (PNEC) derived from ecotoxicity studies to characterise the risk associated with use of mulch film. The model, if validated by comparison of the calculated PECs with the Measured Environmental Concentrations (MECs), determined by detection and quantification of biodegradable mulch film residues in soil, may provide a valuable tool for the ecological risk assessment of biodegradable mulch films.

Plant metabolic responses to soil herbicide residues differ under herbivory in two woodland strawberry genotypes

The use of glyphosate-based herbicides (GBHs) to control weeds has increased exponentially in recent decades, and their residues and degradation products have been found in soils across the globe. GBH residues in soil have been shown to affect plant physiology and specialised metabolite biosynthesis, which, in turn, may impact plant resistance to biotic stressors.In a greenhouse study, we investigated the interactive effects between soil GBH residues and herbivory on the performance, phytohormone concentrations, phenolic compound concentrations and volatile organic compound (VOC) emissions of two woodland strawberry (Fragaria vesca) genotypes, which were classified as herbivore resistant and herbivore susceptible. Plants were subjected to herbivory by strawberry leaf beetle (Galerucella tenella) larvae, and to GBH residues by growing in soil collected from a field site with GBH treatments twice a year over the past eight years.Soil GBH residues reduced the belowground biomass of the susceptible genotype and the aboveground biomass of both woodland strawberry genotypes. Herbivory increased the belowground biomass of the resistant genotype and the root–shoot ratio of both genotypes. At the metabolite level, herbivory induced the emission of several VOCs. Jasmonic acid, abscisic acid and auxin concentrations were induced by herbivory, in contrast to salicylic acid, which was only induced by herbivory in combination with soil GBH residues in the resistant genotype. The concentrations of phenolic compounds were higher in the resistant genotype compared to the susceptible genotype and were induced by soil GBH residues in the resistant genotype.Our results indicate that soil GBH residues can differentially affect plant performance, phytohormone concentrations and phenolic compound concentrations under herbivore attack, in a genotype-dependent manner. Soil GBH altered plant responses to herbivory, which may impact plant resistance traits and species interactions. With ongoing agrochemical pollution, we need to consider plant cultivars with better resistance to polluted soils while maintaining plant resilience under challenging environmental conditions.

Influence of Residue Soil on the Properties of Fly Ash-Slag-Based Geopolymer Materials for 3D Printing.

This study investigates the impact of residue soil (RS) powder on the 3D printability of geopolymer composites based on fly ash and ground granulated blast furnace slag. RS is incorporated into the geopolymer mixture, with its inclusion ranging from 0% to 110% of the combined mass of fly ash and finely ground blast furnace slag. Seven groups of geopolymers were designed and tested for their flowability, setting time, rheology, open time, extrudability, shape retention, buildability, and mechanical properties. The results showed that with the increase in RS content, the fluidity of geopolymer mortar decreases, and the setting time increases first and then decreases. The static yield stress, dynamic yield stress, and apparent viscosity of geopolymer mortar increase with the increase in RS content. For an RS content between 10% and 90%, the corresponding fluidity is above 145 mm, and the yield stress is controlled within the range of 2800 Pa, which meets the requirements of extrusion molding. Except for RS-110, geopolymer mortars with other RS contents showed good extrudability and shape retention. The compressive strength of 3D printing samples of geopolymer mortar containing RS has obvious anisotropy.

Soil residues and crop accumulation of organophosphorus and pyrethroid pesticides in agricultural fields in Shaanxi, China

Soil residues and crop accumulation of organophosphorus and pyrethroid pesticides in agricultural fields in Shaanxi, China

Effects of soil crust on the collapsing erosion of colluvial deposits with granite residual soil

Effects of soil crust on the collapsing erosion of colluvial deposits with granite residual soil

Sorption and mobility assessment of tembotrione in soils of upper, trans and middle Gangetic plain zones of India.

The presence of undesired agrochemicals residues in soil and water poses risks to both human health and the environment. The behavior of pesticides in soil depends both on the physico-chemical properties of pesticides and soil type. This study examined the adsorption-desorption and leaching behavior of the maize herbicide tembotrione in soils of the upper (UGPZ), trans (TGPZ) and middle Gangetic plain zones of India. Soil samples were extracted using acetone followed by partitioning with dichloromethane, whereas liquid-liquid extraction using dichloromethane was used for aqueous samples. Residues of tembotrione and its metabolite TCMBA, {2-chloro-4-(methylsulfonyl)-3-[(2,2,2-trifluoroethoxy) methyl] benzoic acid}, were quantified using liquid chromatography-tandem mass spectrometry. The data revealed that tembotrione adsorption decreased with increasing pH and dissolved organic matter but increased with salinity. The maximum adsorption occurred at pH4, 0.01m sodium citrate and 4g/L NaCl, with corresponding Freundlich constants of 1.83, 2.28 and 3.32, respectively. The hysteresis index <1 indicated faster adsorption than desorption. Leaching studies under different flow conditions revealed least mobility in UGPZ soil and high mobility in TGPZ soil, consistent with groundwater ubiquity scores of 4.27 and 4.81, respectively. Soil amendments decreased tembotrione mobility in the order: unamended > wheat straw ash > wheat straw > farm yard manure > compost. The transformation of tembotrione to TCMBA and its mobility in soil columns were also assessed.

Decontamination of Chlorpyrifos Residue in Soil by Using Mentha piperita (Lamiales: Lamiaceae) for Phytoremediation and Two Bacterial Strains.

This study utilizes Mentha piperita (MI) for the first time to investigate the uptake and translocation of chlorpyrifos (CPF; 10 µg g-1) from soil, introducing a new approach to improve the efficacy of this technique, which includes using biosurfactants (Bacillus subtilis and Pseudomonas aeruginosa) at 107 CFU/mL to degrade CPF under greenhouse conditions. Moreover, antioxidant enzymes, including superoxide dismutase (SOD) and peroxidase (Prx), and oxidative stress due to hydrogen peroxide (H2O2) and malondialdehyde (MDA) in MI roots and leaves were evaluated under CPF stress. Our results demonstrated that amending soil with MI and B. subtilis followed by P. aeruginosa significantly reduced CPF levels in the soil (p > 0.05) and enhanced CPF concentrations in MI roots and leaves after 1, 3, 7, 10, and 14 days of the experiment. Furthermore, CPF showed its longest half-life (t1/2) in soil contaminated solely with CPF, lasting 15.36 days. Conversely, its shortest half-life occurred in soil contaminated with CPF and treated with MI along with B. subtilis, lasting 4.65 days. Soil contaminated with CPF and treated with MI and P. aeruginosa showed a half-life of 7.98 days. The half-life (t1/2) of CPF-contaminated soil with MI alone was 11.41 days. A batch equilibrium technique showed that B. subtilis is better than P. aeruginosa for eliminating CPF from soil in In vitro experiments. Notably, CPF-polluted soil treated with coadministration of MI and the tested bacteria improved the activities of SOD and Prx and reduced H2O2 and MDA compared with CPF-polluted soil treated with MI alone. Our findings demonstrated that using B. subtilis and P. aeruginosa as biosurfactants to augment phytoremediation represents a commendable strategy for enhancing the remediation of CPF contamination in affected sites while reducing the existence of harmful pesticide remnants in crop plants.