Residual Monomer Release Research Articles

Cytotoxicity, chlorhexidine and residual monomer release from heat- polymerized denture base resin modified by polyethylene glycol (PEG)and chlorhexidine (CHX).

Cytotoxicity, chlorhexidine and residual monomer release from heat- polymerized denture base resin modified by polyethylene glycol (PEG)and chlorhexidine (CHX).

Mechanical characterization and cytocompatibility of linoleic acid modified bone cement for percutaneous cement discoplasty

Minimally invasive spine treatments have been sought after for elderly patients with comorbidities suffering from advanced degenerative disc disease. Percutaneous cement discoplasty (PCD) is one such technique where cement is injected into a degenerated disc with a vacuum phenomenon to relieve patients from pain. Adjacent vertebral fractures (AVFs) are however an inherent risk, particularly for osteoporotic patients, due to the high stiffness of the used cements. While low-modulus cements have been developed for vertebroplasty through the addition of linoleic acid, there are no such variations with a high-viscosity base cement, which is likely needed for the discoplasty application.Therefore, a low-modulus polymethyl methacrylate was developed by the addition of 12%vol. linoleic acid to a high-viscosity bone cement (hv-LA-PMMA). Initial experimental validation of the cement was performed by mechanical testing under compression over a period of 24 weeks, after storage in 37 °C phosphate buffer saline (PBS) solution. Furthermore, cement extracts were used to evaluate residual monomer release and the cytotoxicity of hv-LA-PMMA using fibroblastic cells.Relative to the base commercial cement, a significant reduction of Young’s modulus and compressive strength of 36% and 42% was observed, respectively. Compression-tension fatigue tests at 5 MPa gave an average fatigue limit of 31,078 cycles. This was higher than another low-modulus cement and comparable to the fatigue properties of the disc annulus tissue. Monomer release tests showed that hv-LA-PMMA had a significantly higher release between 24 h and 7 days compared to the original bone cement, similarly to other low-modulus cements. Also, the control cement showed cytocompatibility at all time points of extract collection for 20-fold dilution, while hv-LA-PMMA only showed the same for extract collections at day 7. However, the 20-fold dilution was needed for both the control and the hv-LA-PMMA extracts to demonstrate more than 70% fibroblast viability at day 7.In conclusion, the mechanical testing showed promise in the use of linoleic acid in combination with a high-viscosity PMMA cement to achieve properties adequate to the application. Further testing and in vivo studies are however required to fully evaluate the mechanical performance and biocompatibility of hv-LA-PMMA for possible future clinical application.

Evaluation of the amount of residual monomer released from different flowable composite resins

Today, resin materials are used in the restoration of permanent and deciduous teeth or as fissure sealants. The materials can contain different types of monomers (Bis-GMA, UDMA, TEGDMA). These monomers can be released into the oral cavity after polymerization. Residual monomers released from resin-containing restorative materials after polymerization have been reported to have negative effects on mechanical properties. The aim of our study is to evaluate the amount of residual monomers released after polymerization of different flowable composite resin materials using two different modes of LED light source. Composite disc samples (8 mm diameter/2 mm depth) prepared for each material group were polymerized using two different modes of the LED light device (Standard mode and extra power mode). HPLC (High Performance Liquid Chromatography) device was used to measure the amount of residual monomer release at 1 h, 1 day, 3 days and 7 days periods. Pairwise comparison of the differences between the materials was performed by Post-hoc test. For each residual monomer, the Kruskal Wallis test was used to analyze the difference between the materials in standard mode and the difference between the materials in extra power mode. According to the results of the study; Grandio flow flowable composite showed the highest release of TEGDMA and Bis-GMA while SDR® Flow flowable composite showed the lowest release of TEGDMA, Bis-GMA and UDMA. For all materials, the extra power mode resulted in more residual monomer release. TEGDMA and Bis-GMA release was detected in all tested flowable composites at all time periods.

Residual Monomer Release after the Polymerization of Different-Colored Flowable Compomer.

Compomers are restorative materials commonly used in pediatric dentistry. It is important to investigate the monomer release of materials used especially in pediatric patients. The aim of this study was to evaluate the residual monomer released from different-colored flowable compomers at five polymerization times. Three experimental groups were formed: Group 1: pink flowable compomer (Twinky Star, VOCO, Germany), Group 2: blue flowable compomer (Twinky Star, VOCO), Group 3: white flowable compomer, A2 color of Dyract XP (Dentslpy DeTrey, Konstanz, Germany). For each group, samples were prepared using standard cylindrical Teflon molds (n = 10), and the compomers were cured using a light-emitting diode (LED) source in accordance with the manufacturer's recommendations. During each time period, the number of residual monomers: bisphenol-A glycidyl methacrylate (bis-GMA), urethane dimethacrylate (UDMA), hydroxyethyl methacrylate (HEMA), and triethylene glycol dimethacrylate (TEGDMA) were determined with high-performance liquid chromatography (HPLC). All data were analyzed statistically. In all groups, the amount of residual monomers increased after 1-h release periods (P < 0.05). For 2 weeks, the total residual monomers released were determined at most in Group 3 and at least in Group 1. However, there was no significant difference between the groups in terms of released amounts of monomers (P > 0.05). According to the study, when the total amount of residual monomer released from all groups was compared, no difference was found and it was observed that none of them exceeded the toxic dose. Flowable colored compomers can be used as safely as white compomers in pediatric dentistry.

Evaluation of biocompatibility of four different one-step self-etching adhesives by animal experimental method

Aims: The success of materials used in dentistry is closely related to their biological safety. In recent years, there has been growing concern about the biocompatibility of self-etch adhesives, which are frequently used in restorative dental treatment. The aim of this study is to compare the biocompatibility of four different single-stage self-etch adhesive materials (Prime &amp; Bond One Select, Optibond All-in-One, Clearfil Universal Bond, and Single Bond Universal) with the animal test method and evaluate them histopathologically. Methods: The experimental materials were polymerized by filling 10 mm-long, 2 mm-diameter polyurethane tubes. For each adhesive, 21 tubes were used. The tubes were placed in the subcutaneous pockets formed by four different incisions in the backs of 21 adult male albino rats. On the 7th, 30th, and 60th days, rats were randomly divided into three groups. At the end of these durations, the tube and the surrounding 2 cm2 of tissue were excised together. The sections were taken into preparation and stained with hematoxylen and eosin. The severity of inflammation, inflammatory cell count, and fibrous capsule thickness were evaluated histologically by light microscopy. Results: On the 7th day of samples, lesions due to acute inflammation, edema, and PMNL infiltration were observed. In the 30th-day samples, it was observed that inflammation decreased against all materials and fibrocollagen tissue increased. On the 60th day, granulation and fibrous tissue increased due to regeneration and reparation processes. On the 60th day, the number of inflammatory cells decreased significantly compared to the 7th day. When the fibrous tissue formation was evaluated, it was found that the 7th day score was significantly lower than the 60th day score. Conclusion: The severity of the inflammatory reaction at the beginning of all materials can be explained by the effects of residual monomer release and surgical trauma.

Comparison of residual monomer amounts released from indirect bonding adhesives.

To quantify the amount of residual monomer released from orthodontic adhesives used in the indirect bonding technique and compare it to a direct bonding composite resin. Five hundred stainless steel orthodontic brackets were bonded on bovine incisors using five groups of bonding resins: Transbond XT (TXT), Transbond Supreme LV (SLV), Sondhi Rapid-Set (SRS), Transbond IDB (IDB), and Custom I.Q. (CIQ). Liquid samples were gathered on the first, seventh, 21st, and 35th days. Residual monomer release was measured from the liquid samples with a liquid chromatography device. In addition, the amount and shape of the adhesive between the tooth surface and the bracket base was evaluated using obtained electron microscopy images. The data were analyzed using analysis of variance, and a Tukey post-hoc test was applied. Hydroxyethylmethacrylate and bisphenol A-glycidyl methacrylate monomers were released by all study groups. Urethane-dimethacrylate was released from the TXT, SLV, IDB, and CIQ groups. Triethylene glycol dimethacrylate was released from TXT, SLV, IDB, and SRS groups. The amount of total monomer release was higher in chemically cured adhesives than in light-cured adhesives. Among the chemically cured adhesives, premix adhesives had the highest amount of total monomer release. The light-cured adhesives had less thickness. Light-curing adhesives have significantly less monomer release than chemically polymerized adhesives.

Degree of conversion and residual monomer elution of 3D-printed, milled and self-cured resin-based composite materials for temporary dental crowns and bridges

The aim of this work was to investigate the elution of residual monomers as a function of the manufacturing process, which are CAD/CAM manufacturing, self-curing and 3D printing. The experimental materials used consisted of the base monomers TEGDMA, Bis-GMA and Bis-EMA and 50 wt.% fillers. Additionally, a 3D printing resin without fillers was tested. The elution of the base monomers into the different media (water, ethanol and ethanol/water (75/25 vol. %)) at 37 °C over a period of up to 120 d as well as the degree of conversion (DC) by FTIR were investigated. No monomer elution could be detected in water. Most residual monomers in both other media were released from the self-curing material whereas the 3D printing composite released relatively little. The CAD/CAM blanks released hardly any quantitatively detectable amounts of monomers. Relative to the base composition, TEGDMA eluted less than Bis-GMA and Bis-EMA. DC did not correlate with residual monomer release; thus, leaching was determined not only by the amount of residual monomers present but by further factors as possibly network density and structure. The CAD/CAM blanks and the 3D printing composite showed similar high DC but lower residual monomer release from the CAD/CAM blank, likewise the self-curing composite and the 3D printing resin exhibited similar DC but different monomer elution. In terms of residual monomer elution and DC, the 3D printing composite seems promising as a new material class for the use as temporary dental crowns and bridges.Graphical

Effect of topical fluoride applications on residual monomer release from resin-based restorative materials

BackgroundThe effects of topical fluoride applications on the release of monomer ingredients from resin-based dental materials by immersion in various extraction solutions are unclear. The aim of this study was to determine the effect of topical fluorides (APF and NaF) on the elution of residual monomers (Bis-GMA, TEGDMA, UDMA, and HEMA) from resin-based materials.MethodsNinety specimens were prepared, 30 bulk-fill composite resin, 30 nanohybrid universal composite resin, and 30 polyacid-modified composite resin (compomer). These were randomly divided into three groups based on fluoride application procedures. Each specimen was kept in 75% ethanol solution, and residual monomers released from materials were analyzed using high-performance liquid chromatography (HPLC) after 10 min, 1 h, 24 h, and 30 days. The groups were compared using the Mann Whitney U and Kruskal Wallis tests. Measurements were analyzed using the Friedman and Wilcoxon signed-rank tests.ResultsFluoride applications generally had no considerable effect on the amount of residual monomer released from resin-based restorative materials. The amount of monomer release after topical APF application was similar to the release in the control group and was lower than the release in the NaF group. The release of monomers from the resin-based material used in the study did not approach toxic levels at the applied time intervals. The compomer released lower amounts of monomer than other resin-based materials.ConclusionsFluoride applications do not increase monomer release from resin-based restorative materials. However, compomers should be employed by clinicians due to their lower monomer release compared to other resin restorative materials. The release of monomers from all the resin-based materials did not approach toxic levels at the applied time intervals.

Analysis of Monomer Release from Different Composite Resins after Bleaching by HPLC.

(1) Background: This study aimed to examine the effect of bleaching agents on the release of triethylenae glycol dimethacrylate, 2-hydroxyethyl methacrylate, bisphenol A, urethane dimethacrylate, and bisphenol A-glycidyl methacrylate monomers, which are released from different composite resins, using the high-performance liquid chromatography (HPLC) method. (2) Methods: Ninety disc-shaped specimens were produced and immersed in artificial saliva. After different bleaching applications [office type bleaching (OB) and home type bleaching (HB)], the specimens were immersed in a 75 wt% ethanol/water solution, and the released monomers were analyzed by HPLC at predefined time intervals: 1, 7, and 28 days. The Kruskal-Wallis and Mann-Whitney U tests were conducted for statistical analysis (p = 0.05). (3) Results: The monomers were released at all times from all composite specimens. The monomer release was increased over time. The highest monomer release was detected on day 28. Bleaching applications affected monomer release. No statistical difference was found between OB and HB applications (p &gt; 0.05). The most released monomer was Bisphenol-A in all composites. (4) Conclusion: Given that a residual monomer release from composite resins has a toxic effect and that bleaching treatments increase this release, a treatment protocol should be made in accordance with the manufacturer's instructions.

Comparative chemical properties, bioactivity, and cytotoxicity of resin-modified calcium silicate-based pulp capping materials on human dental pulp stem cells.

This study investigated the cytotoxicity, the residual monomer release, degree of conversion (DC), calcium ion (Ca2+) release, and crystal structure of TheraCal PT (ThPT) by comparison with TheraCal LC (ThLC) and mineral trioxide aggregate (MTA). The cytotoxicity of the cured materials was evaluated on human dental pulp stem cells (hDPSCs) isolated from third molars by the water-soluble tetrazolium salt (WST-1) method. The monomer release and DC of the resin-containing materials were analyzed by high-performance liquid chromatography (HPLC) and Fourier transform infrared (FTIR), respectively. The chemical composition and Ca2+ release of the materials were determined by scanning electronic microscopy-energy-dispersive spectroscopy (SEM-EDS), X-ray diffractometry (XRD), and inductively coupled plasma-optical emission spectroscopy (ICP-OES), respectively. Statistical differences were evaluated with one-way ANOVA, repeated measure ANOVA, and the Tukey test (p < 0.05). MTA showed significantly lower cytotoxicity than either ThLC or ThPT after 1, 3, and 7days (p < 0.05). TEGDMA release of ThPT is significantly higher than ThLC (p < 0.05). All materials showed calcium Ca2+ release, with MTA significantly higher than the others (p < 0.05). MTA showed low cytotoxicity and high Ca2+ release compared to ThLC and ThPT. The cytotoxicity and residual monomer release of ThLC and ThPT may raise concerns about the viability of hDPSCs. Further investigations with the use of in vivo research models are required to validate in vitro bioactivity properties and the potential adverse biological effects of ThLC and ThPT on hDPSCs.

Evaluation of residual monomer release after polymerization of different restorative materials used in pediatric dentistry

BackgroundThe choice of the restorative resin material to be used in pediatric dentistry is of a great importance due to the cytotoxic effects caused by residual monomers. In this study, it was aimed to investigate the amount of residual monomer released over time from different resin-based restorative materials, which are widely used in pediatric dentistry, by using high performance liquid chromatography with photodiode array detector (HPLC–PDA).MethodsThe compomers in all colors (Twinky Star and Glasiositte A2), two composites with different hybrid properties (Arabesk-GrandioSO), and RMGIC (Ionolux) samples with 2 × 5 mm diameters were prepared. The samples were polymerized with an LED light unit (CELALUX 2, VOCO, Cuxhaven, Germany) and then finishing-polishing procedures were applied. A total of 156 samples were obtained, 13 samples in each of the 12 groups. The amount of residual monomer (BIS-GMA; HEMA, TEGDMA, UDMA) (µg/mL) released into the 75% ethanol solution was determined at different times, (1st hour, 1st, 7th, 14th, and 21st day) by using HPLC–PDA.ResultsThe residual monomer release continued on day 21 and BIS-GMA was the most released monomer in all groups. HEMA release showed a maximum increase in all the materials at day 7. The highest amount of residual monomer was detected in the gold-colored compomer. HEMA and BIS-GMA release from RMGIC was less than others in all time frames.ConclusionsThe color and composition of resin-based restorative materials affect the amount of residual monomer. Pediatric dentists should prefer gold-colored compomers less than others as a restorative material, especially in deep cavities. More studies are needed about the subject.

Monomer Release from Dental Resins: The Current Status on Study Setup, Detection and Quantification for In Vitro Testing.

Improvements in mechanical properties and a shift of focus towards esthetic dentistry led to the application of dental resins in various areas of dentistry. However, dental resins are not inert in the oral environment and may release monomers and other substances such as Bisphenol-A (BPA) due to incomplete polymerization and intraoral degradation. Current research shows that various monomers present cytotoxic, genotoxic, proinflammatory, and even mutagenic effects. Of these eluting substances, the elution of BPA in the oral environment is of particular interest due to its role as an endocrine disruptor. For this reason, the release of residual monomers and especially BPA from dental resins has been a cause for public concern. The assessment of patient exposure and potential health risks of dental monomers require a reliable experimental and analytical setup. However, the heterogeneous study design applied in current research hinders biocompatibility testing by impeding comparative analysis of different studies and transfer to the clinical situation. Therefore, this review aims to provide information on each step of a robust experimental and analytical in vitro setup that allows the collection of clinically relevant data and future meta-analytical evaluations.

Development of New Experimental Dental Enamel Resin Infiltrants-Synthesis and Characterization.

The aim of the present study was to obtain experimental infiltration materials, intended for the treatment of dental white spots, and to investigate them. Two series of infiltrants (P1–P6)/(P1F–P6F) were obtained, based on different monomer mixtures, without/with glass filler (with fluoride release ability). Each infiltrant from the second series contained the same amount of glass powder, and each infiltrant from the (P–PF) group contained the same resin composition. The characteristics of the experimental infiltrants were investigated by degree of conversion (DC), mechanical strength, water sorption (WS), and fluoride release, in addition to residual monomer for (P1F–P6F) infiltrants. The results were compared with those obtained for commercial Icon infiltrant. For the experimental infiltrants, without/with filler, the recorded DC was in the range of 58.27–89.70%/60.62–89.99%, compared with Icon (46.94%) 24 h after polymerization. The release of fluoride depends on the permeability of the polymer matrix, with respect to the water sorption, which may help to diffuse ions in the storage medium but which can also influence the release of residual monomers. The highest flexural strengths were recorded for the (TEGDMA/HEMA/Bis-GMA) infiltrants (133.94 ± 16.389 MPa/146.31 ± 7.032 MPa). The best experimental infiltrants were P2 and P2F (Bis-GMA/HEMA/TEGDMA).

Physico-chemical properties and biocompatibility of in situ-hardening polylactide/nano hydroxyapatite composite for bone substitute

Polylactide (PLA) and hydroxyapatite (HA) composite is a promising material for biomedical applications. The bioresorbability and adequate strength of PLA in combination with the bioactivity of HA clearly provide prospective properties for resorbable bone substitute. The material, however, requires to be shaped prior to surgery, which impedes its practical use, particularly in complex shaped bone defects. In this study, a novel combination of star-shaped and linear PLA powder with various amounts of nano HA, and triethylene glycol dimethacrylate liquid was developed as an in situ forming cement for bone substitute. The effects of different amounts of initiator (0.5 and 1 wt%) and nano HA powder (5, 10, and 15 wt%) on various properties of the composite cement, including handling properties, degree of conversion, gel fraction, residual monomer toxicity and osteoblast differentiation were studied. The in vitro degradation was also examined in terms of weight and mechanical loss. The results demonstrated that the amount of initiator had a great effect on the handling properties of the cement, however, the HA content did not have any significant effect. The increasing HA content slowed down the degradation rate of the cement. All cement formulations in this study exhibited good network forming and ability to induce osteoblast differentiation with negligible residual monomer release to harm cells. The optimal cement formulation was found to be at 15 wt% HA, which allowed for in situ hardening with satisfactory biocompatibility and mechanical support for bone regeneration during deterioration.

Evaluation of Residual Monomers Eluted from Pediatric Dental Restorative Materials.

Unreacted monomers eluted from resin-based restorative materials have been considered a reason of local and systemic adverse reactions. This study was designed to determine the effect of finishing and polishing procedures on the elution of Bis-GMA, TEGDMA, UDMA, and HEMA monomers from compomer and bulk-fill composite resins. Bulk-fill composite (3M ESPE GmbH, Seefeld, Germany) and compomer (Dentsply DeTrey GmbH, Konstanz, Germany) specimens with 3 × 4 mm diameters were prepared. The specimens were randomly divided into two groups, and finishing-polishing procedures were applied only to the experimental groups. Release of residual monomers was analyzed by using High-Performance Liquid Chromatography (HPLC) after 24, 48, and 72 hours. Repeated measures ANOVA and Tukey post hoc tests were used for comparisons. Finishing and polishing procedures had a significant effect on reducing the quantity of UDMA release in the Filtek™ Bulk Fill composite and Bis-GMA, HEMA, and TEGDMA in the Dyract XP compomer (p < 0.05). The restorative materials investigated here are not chemically stable after polymerization, and concentrations of eluted monomers may reach critical toxicity levels even after one restoration placement. Finishing and polishing procedures are mandatory to reduce residual monomers.

Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

Light activated resin-based composites are the most accepted and used materials among clinicians. The aim of this study is to determine the amount of residual monomer released from nanofiller composite resins for different polymerization times and storage periods in vitro. To this purpose, Tetric Ceram (Ivoclar, Liechtenstein), Clearfil Majesty Posterior (Kuraray, Japan), Grandio (VOCO, Germany), and Filtek Ultimate Universal (3M, USA) were used as nanofiller resin composites samples. Four groups ( n = 40 , diameter: 5 mm, thickness: 2 mm) of each material were fabricated, and each group was exposed to three different polymerization time (10, 20 and 40 sec). High-performance liquid chromatography (HPLC) was used to measure the amount of monomers released over 1, 15, and 30 days. The highest amount of monomer release was seen in Tetric EvoCream composite, while the least monomer release was seen in Clearfil Majesty composite. Regardless of the polymerization time, material, or storage period, the highest amount of eluted monomer was Bis-GMA. It is observed that there is no statistically significant difference between various polymerization times. Monomer release reached its highest level on the 15th day and decreased on the 30th day for all composites. Polymerization time did not affect the monomer release from the composites, but the type of the monomers and concentration of the filler used in the composites affected the amount of released monomers. The use of TEGDMA (co)monomer reduced the monomer release.

Effect of layer thickness on residual monomer release in polymerization of bulk-fill composites

The aim of the present study was to investigate and compare the quantity of residual monomers leached from the bulk-fill composites with different compositions polymerized at varying layer thickness. Three bulk-fill (X-tra-fil, Beautifil Bulk Restorative, Fill-Up) and a nanohybrid composite (Filtek Z550) were used for the study. The composite resin samples were prepared with a stainless steel mold. For each composite, two groups were constructed. The samples in the first group were prepared using the 2 + 2 mm layering technique. In the second group, the composite samples were applied as a 4 mm-thick one layer and polymerized. Then, each composite samples were kept in a 75% ethanol solution and residual monomers released from composite resins were analyzed with an HPLC device after 24hour and 1 month. The data were analyzed using Kruskal-Wallis and Mann-Whitney U tests. Except the Fill-Up, all of residual monomer elution from the bulk-fill composites was significantly affected by the layer thickness (p &lt; 0.05). The greatest monomer release was detected at 1 month after polymerization as a single 4 mm layer for Beautifil Bulk Restorative. Fill-Up composite showed similar residual monomer release in polymerization at different layer thicknesses compared to other composite resins. In the 2 + 2 mm layering technique, the least monomer elution was detected in the Filtek Z550 composite group. While Bis-GMA was the most released monomer in X-tra fil composite, UDMA was the most released monomer in all other composite resins. During polymerization of the bulk-fill composite, the layer thickness of the composite applied may affect the amount of residual monomers released from the composite resins. Conventional composites may release less monomer than bulk-fill composites when used with layering.

Evaluation of Residual Monomer Release After Polymerization of Colored Compomer Materials

Objective: To evaluate the amount of residual monomers released after polymerization by the compomers in different colors and viscosities over time. Material and Methods: The compomer samples of different colors and viscosities (flowable compomers; blue-pink and packable compomers; A2-blue-pink-gold) were prepared in molds with an inner diameter of 5 mm and a height of 2 mm. In polymerization of samples, a LED unit was used. The amount of monomers released from the samples kept in 75% ethanol/water solution was measured by a high-performance liquid chromatography (HPLC) instrument in the 10th minute, in the 1st hour, and in the 1st, 7th, and 14th days. For statistical analyses, the paired sample t-test, independent sample t-test, and one-way ANOVA with Tukey's post hoc test were used. Results: The amount of residual monomers released from all materials increased over time. At the end of the 14th day, the most released monomer from all compomer samples was BisGMA. The total amounts of released monomers from the packable compomers were Gold>A2>blue>pink. The amount of residual monomers released from flowable compomers was higher in blue than in pink. Conclusion: The color and the viscosity are the factors affecting the residual monomer release in compomers.

Effect of Degree of Conversion of Different Resin Composite Monomers (Methacrylate and Silorane) on Two Caries-Associated Bacteria

Abstract Background and Objectives: The biological response of oral bacteria to dental restorative polymer composites is mediated by the release of unpolymerized residual monomers. The aims of the present study were first, evaluating the effect of composite elutes from methacrylate composite resins and silorane-based composites on two cariogenic bacterial pathogens: Streptococcus mutans and Lactobacillus acidophilus and second, determination of degree of conversion (DC) of standardized discs made from the low-shrinkage silorane-based composite (FiltekTM P90) resin and methacrylate-based composite resin (FiltekTM Z350XT nanocomposite, FiltekTM Z350 Flow) using Fourier transform infrared spectroscopy (FTIR). Materials and Methods: Thirty-six composite specimens were prepared for the agar diffusion test and dissolved in a solvent (dimethyl sulfoxide) to attain a suspension, which was used to assess the effect of the residual monomers from methacrylate composite resins and silorane-based composites on the growth of S. mutans and L. acidophilus. To avoid more dilutions of the specimens, the colonies of bacteria were counted by the naked eye. Thirty composite specimens of 2 mm × 6 mm were polymerized for DC test, and the DC was measured using FTIR, also for unpolymerized composite resin. Results: The growth of S. mutans bacteria was inhibited when cultured with Filtek Z350 Flow composite resin, while the growth of L. acidophilus bacteria was stimulated by the Filtek Silorane p90 and Filtek Z350 Flow composite resin. The DC test with the Filtek Silorane composite resin showed highest DC (58%), then the Filtek Z350XT (Nanocomposite) (42%) followed by the Filtek Z350 Flow (Flowable) showed lowest (35%). Conclusions: Our results demonstrate that composite filling materials have a versatile nature of effect on oral pathogenic bacteria, which could modulate their pathogenesis. Dentists may thus select the appropriate type of composite filling according to the caries susceptibility of patients.

Evaluation of the effect of temperature and time on the conversion factor of acrylic resin denture base material

Aim: To evaluate the effect of change in time, temperature (increase or decrease) on the degree of conversion and residual monomer release of the vertex heat cured acrylic resin. Material and Methods: The total number of samples (176) were divided into three main groups cured according to ADA and three curing cycles with modifications in time and temperature, degree of conversion and residual monomer properties were measured for all the samples. Results: Analyzed statistically by (Descriptive statistics, ANOVA and Duncan’s multiple range test) and showed that both changes in time and temperature showed significant changes in the degree of conversion, residual monomer. Conclusion: The rapid simplified and regular manufacturer’s instruction curing cycles showed the best results regarding the degree of conversion, residual monomer. Keywords: Temperature, Time, Conversion factor, Acrylic resin.