Alternative water disinfectants to chlorination need to be identified because its effectiveness is limited by water pH and potentially carcinogen byproducts resulted from chlorination and organic compound’s reaction. The first study aimed to evaluate the effect ofdifferentdrinking water chemical disinfection treatments on water quality, its potential hazard effects on animal health, water and feed consumption, and apparent total tract digestibilityindairy beef bulls fed high-concentrate diets.For 224 days, 24Holstein bulls (176 ± 16.3 kg BW, and 149 ± 5.8 days of age) wereindividually assignedto one of four treatments with different drinking water chemical disinfectants: without disinfection (CTR); acidification and chlorination (ACCHL; 0.65 mL/L H3PO4and 0.14 mL/L NaClO 15%); hydrogen peroxide (PER; 0.15 mL/L); and chlorine dioxide (DIO; 2.50 mL/L). Data were analysed with amixed-effectsmodel. Treatments affectedthe chemical characteristics of the water: in ACCHL, pH was 6.60 and free residual chlorine was 0.75 mg/L; in PER, H2O2was 10.6 mg/L; and in DIO, ClO2was 0.52 mg/L. Water physicochemical quality parameters in all treatments were belowmaximal thresholds established forsafe water consumption by the Water Safety Royal Decree (RD 140/2003). In addition,totalcoliform count of treated waters was reduced (P = 0.01) compared with CTR; moreover, ACCHL and DIO treatments were more effectiveinreducing total coliform count than PER. Dry matter intake tended (P= 0.07) to increase in DIO compared with CTR. Treatments didnotaffect blood parameters norapparent total tract digestibility. The second study aimed to evaluate the potential benefit on animal performance of two drinking water disinfectants under commercial conditions in dairy beef crossbred Holstein bulls fed high-concentrate diets. Ninety-six animals (307 ± 4.4 kg BW, and 224 ± 1.8 days of age) were allocatedtosix pens for 140 days and assigned to one of two treatments: ACCHL,most common water disinfectant, and DIO. Data were analysed with a mixed-effects model. Water total coliform countand water consumption weresimilar between treatments. Concentrateintake was greater (P= 0.02) inACCHL for the last 14 study days. Growth performance and carcass quality were similar between treatments. In summary, acidification and chlorination, H2O2, and ClO2as drinking water disinfectants in dairy beef bullshad gooddisinfecting activity without detrimental effects on health and nutrient digestibility, and performance.