The absolute accuracy of antenna brightness temperatures (TDR) from the Advanced Technology Microwave Sounder (ATMS) onboard the Suomi National Polar-orbiting Partnership satellite is estimated using the Constellation Observing System for Meteorology, Ionosphere, and Climate radio occultation (RO) data as input to the U.S. Joint Center of Satellite Data Assimilation community radiative transfer model (RTF). It is found that the mean differences (e.g., biases) of observed TDR observations to GPS RO simulations are positive for channels 6, 10-13 with values smaller than 0.5 K and negative for channels 5, 7-9 with values greater than -0.7 K. The bias distribution is slightly asymmetric across the scan line. A line-by-line RTF is used to further understand the sources of errors in forward calculations. It is found that, for some channels, the bias can be further reduced in a magnitude of 0.3 K if the accurate line-by-line simulations are used. With the high quality of GPS RO observations and the accurate RTF, ATMS upper level temperature sounding channels are calibrated with known absolute accuracy. After the bias removal in ATMS TDR data, it is shown that the distribution of residual errors for ATMS channels 5-13 is close to a normal Gaussian one. Thus, for these channels, the ATMS antenna brightness temperature can be absolutely calibrated to the sensor brightness temperature without a systematic bias.