Residual Debris Research Articles

In Vivo Clearance of Apoptotic Debris From Tumor Xenografts Exposed to Chemically Modified Tetrac: Is There a Role for Thyroid Hormone Analogues in Efferocytosis?

Apoptosis is induced in cancer cells and tumor xenografts by the thyroid hormone analogue tetraiodothyroacetic acid (tetrac) or chemically modified forms of tetrac. The effect is initiated at a hormone receptor on the extracellular domain of plasma membrane integrin αvβ3. The tumor response to tetrac includes 80% reduction in size of glioblastoma xenograft in two weeks of treatment, with absence of residual apoptotic cancer cell debris; this is consistent with efferocytosis. The molecular basis for efferocytosis linked to tetrac is incompletely understood, but several factors are proposed to play roles. Tetrac-based anticancer agents are pro-apoptotic by multiple intrinsic and extrinsic pathways and differential effects on specific gene expression, e.g., downregulation of the X-linked inhibitor of apoptosis (XIAP) gene and upregulation of pro-apoptotic chemokine gene, CXCL10. Tetrac also enhances transcription of chemokine CXCR4, which is relevant to macrophage function. Tetrac may locally control the conformation of phagocyte plasma membrane integrin αvβ3; this is a cell surface recognition system for apoptotic debris that contains phagocytosis signals. How tetrac may facilitate the catabolism of the engulfed apoptotic cell debris requires additional investigation.

An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

BackgroundRosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and, more recently, cell wall biogenesis. However, trichome-specific biochemical or -omics analyses require a proper separation of trichomes from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions and suffer from low yield, thereby limiting the spectrum of downstream analyses.ResultsTo take trichome-leaf separation to the next level, we revised a previously proposed method for isolating A. thaliana trichomes by optimizing the mechanical and biochemical specifications for trichome release. We additionally introduced a density gradient centrifugation step to remove residual plant debris. We found that prolonged, yet mild seedling agitation increases the overall trichome yield by more than 60% compared to the original protocol. We noticed that subsequent density gradient centrifugation further visually enhances trichome purity, which may be advantageous for downstream analyses. Gene expression analysis by quantitative reverse transcriptase-polymerase chain reaction validated a substantial enrichment upon purification of trichomes by density gradient centrifugation. Histochemical and biochemical investigation of trichome cell wall composition indicated that unlike the original protocol gentle agitation during trichome release largely preserves trichome integrity. We used enriched and density gradient-purified trichomes for proteomic analysis in comparison to trichome-depleted leaf samples and present a comprehensive reference data set of trichome-resident and -enriched proteins. Collectively we identified 223 proteins that are highly enriched in trichomes as compared to trichome-depleted leaves. We further demonstrate that the procedure can be applied to retrieve diverse glandular and non-glandular trichome types from other plant species.ConclusionsWe provide an advanced method for the isolation of A. thaliana leaf trichomes that outcompetes previous procedures regarding yield and purity. Due to the large amount of high-quality trichomes our method enabled profound insights into the so far largely unexplored A. thaliana trichome proteome. We anticipate that our protocol will be of use for a variety of downstream analyses, which are expected to shed further light on the biology of leaf trichomes in A. thaliana and possibly other plant species.

Effect of Continuous Chelation Irrigation Using DualRinse HEDP+3% NaOCl with or without High-power Sonic Activation on Debris and Smear Layer Removal.

This study aimed to assess the effect of sodium hypochlorite (NaOCl) combined with a novel chelating agent DualRinse HEDP (Medcem GmbH, Weinfelden, Switzerland), a product consisting of 0.9 g of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) powder, with or without high-power sonic activation on debris and smear layer removal. Seventy-five mandibular premolars were divided into 5 groups (n=15) and treated with different irrigation protocols: group 1 (D3N), DualRinse HEDP+3% NaOCl without activation; group 2 (D3NA), DualRinse HEDP+3% NaOCl with activation (EDDY, VDW, Munich, Germany) during the final irrigation; group 3 (3NE), 3% NaOCl+17% Ethylenediaminetetracetic acid (EDTA)+3% NaOCl without activation; group 4 (3NEA), 3% NaOCl+17% EDTA+3% NaOCl with activation during the final irrigation; group 5 (NC), negative control group, 0.9% saline. Samples were analysed by scanning electron microscopy (SEM) to evaluate residual debris and smear layer at 3 levels of the root canal: coronal, middle, and apical. Statistical analysis was performed with a level of significance set at p<0.05. The normality distribution of scores within each group was assessed using Kolmogorov-Smirnov and Shapiro-Wilk tests. A Kruskal-Wallis test followed by multiple comparison tests was used to compare scores among the 5 groups on the apical, middle, and coronal levels of the root canal. A Friedman test followed by multiple comparison tests was used to compare scores within the apical, middle, and coronal levels for each treatment group. Debris score was significantly the lowest for D3NA, followed by D3N, 3NEA and 3NE at all root levels (p<0.05). The smear layer score was significantly the lowest for D3NA, followed by D3N, 3NEA and 3NE only at the apical level, while no significant difference was found in the middle and coronal levels between the groups (p<0.05). DualRinse HEDP resulted in less debris and smear layer compared to the classic approach of NaOCl without activation. Implementing sonic activation further improved debris and smear layer removal. DualRinse HEDP+3% NaOCl improved debris removal at all levels and smear layer elimination at the apical level of the root canal. These results were further enhanced when adding high-power sonic activation. (EEJ-2022-09-116).

Evaluation of Residual Debris and Smear layer After Root Canal Preparation by Three Different Methods: A Scanning Electron Microscopy Study.

This study investigated the amount of debris and smear layer remaining followed chemomechanical preparation using three systems: ProTaper Universal, reciprocating SafeSider, and hand K-Flexofiles with scanning electron microscope (SEM). Sixty-five mandibular molars with mesiobuccal canal curvature (25 to 40°) were extracted and divided into one control group (n=5), and three experimental groups (n=20) according to the preparation method; K-Flexofile, ProTaper Universal and SafeSider instruments. All canals were irrigatedwith 3 ml of 5.25% sodium hypochlorite solution and 3 mL of 17% EDTA. Subsequently, the canals were irrigated with 5 ml of normal saline. Then the teeth were examined under the scanning electron microscope (SEM). Kruskal-Wallis, Dunn-Q Bonferroni, and Friedman tests were used for statistical analysis of results. To assess the accumulation of debris, statistically significant differences were observed only in the coronal area among ProTaper Universal, SafeSider, K-Flexofile, and the control group. (P=0.029). To evaluate the residual smear layer amount, statistically significant differences were observed only in the coronal and middle areas, following the preparation of the canals using ProTaper Universal, SafeSider, and hand K-Flexofiles and control groups (P=0.019). Based on the present in vitro study, we can declare that the canals were utterly cleaned of debris and smear layer in none of the groups. Manual Flexofile and ProTaper Universal groups result in cleaner canal walls than reciprocal SafeSider, in the coronal and middle thirds.

Bridging Work and Global Asias: Stars and Sandbars

Proposals for Global Asias as an emerging field that “bridges” Asian studies and Asian American studies should attend to the residual debris of older understandings of global bridging work. This response explores two motifs for speculating about what bridging work in Global Asias could be: stars, as understood in terms of interimperial constellations, and sandbars, as a metaphor for more local, coalitional, and temporary aggregations.

Landslide susceptibility evolution in an area affected by the 2015 Nepal earthquakes, derived from multitemporal landslide inventories

The Mw 7.8 and Mw 7.3 Nepal earthquakes in 2015 triggered >47,000 landslides and significantly enhanced landslide activity in the affected region in the following years, likely due to a drop in rock strength of the slopes there. This work analyzed the evolution of landslide susceptibility of an area close to the Zhangmu border crossing on the Araniko Highway in Nepal, which was seriously affected by the mainshock, aftershock, and monsoon rainfalls. Using eight landslide inventories we constructed to register landslides triggered by monsoon rainfalls before 2015, the mainshock, the aftershock, and the monsoon rainfalls in 2015–2019, eight susceptibility assessment models were built using the logistic regression method considering 11 control factors of landslides. The changes in the high-susceptibility zones before, during, and after the earthquakes were examined. The backward prediction abilities of the models were checked by taking landsliding and nonlandsliding data in the following years. The results show that the best susceptibility mapping results are obtained based on landslides triggered by the mainshock and the aftershock, which both have successful prediction rates >87%. The high-susceptibility regions have moved from the slope surfaces to channels since 2015, suggesting that transportation of residual debris induced by the strong ground motion resulted from erosion related to the strong post-seismic rainfall and the rivers. A series of attempts using susceptibility models to backward predict landslides in the following years demonstrate that the combination of the susceptibility results of pre-seismic and mainshock-triggered landslides, which have a higher prediction accuracy, has prediction rates >70% until 2018. This study not only will further the understanding of post-seismic landsliding effects, but also will aid in efforts toward future seismic landslide mitigation and post-seismic reconstruction.

Effects of Fire Severity and Woody Debris on Tree Regeneration for Exploratory Well Pads in Jack Pine (Pinus banksiana) Forests

Restoring anthropogenic footprints to pre-disturbance conditions or minimizing their long-term impacts is an important goal in conservation. Many footprints, particularly if left alone, have wide-ranging effects on biodiversity. In Canada, energy exploration footprints result in forest dissection and fragmentation contributing to declines in woodland caribou. Developing cost effective strategies to restore forests and thus conserving the woodland caribou habitat is a conservation priority. In this study, we compared the effects of wildfire and local variation in the amount of residual woody debris on natural regeneration in jack pine on exploratory well pads in Alberta’s boreal forest. Specifically, we investigated how footprint size, fire severity (overstory tree mortality), ground cover of fine and coarse woody debris, and adjacent stand characteristics (i.e., height, age, and cover), affected tree regeneration densities and height using negative binomial count and linear models (Gaussian), respectively. Regeneration density was 30% higher on exploratory well pads than adjacent forests, increased linearly with fire severity on the exploratory well pads (2.2% per 1% increase in fire severity), but non-linearly in adjacent forests (peaking at 51,000 stems/ha at 72% fire severity), and decreased with amount of woody debris on exploratory well pads (2.7% per 1% increase in woody debris cover). The height of regenerating trees on exploratory well pads decreased with fire severity (0.56 cm per 1% increase in fire severity) and was non-linearly related to coarse woody debris (peaking at 286 cm at 9.4% coarse woody debris cover). Heights of 3 and 5 m on exploratory well pads were predicted by 13- and 21-years post-fire, respectively. Our results demonstrate that wildfires can stimulate natural recovery of fire-adapted species, such as jack pine, on disturbances as large as exploratory well pads (500–1330 m2) and that the type and amount of woody debris affects these patterns.

Positively-charged microcrystalline cellulose microparticles: Rapid killing effect on bacteria, trapping behavior and excellent elimination efficiency of biofilm matrix from water environment

Pathogen and biofilm contamination in aqueous systems leave millions of people at risk of waterborne diseases. Herein, to address this issue, a green and highly efficient strategy is developed to concurrently trap and kill bacteria, eliminate the debris and the existing biofilm matrix in water environment via magnetic microparticles. The particles (TPFPs) were prepared from the in-situ deposition of Fe3O4 nanoparticles onto the surface of antibacterial functionalized microcrystalline cellulose (MCC). Noticeably, TPFPs can completely inactivate both S. aureus and E. coli once contacting for 30 min by disrupting the bacterial membrane. Meanwhile, the MCC-based magnetic particles retained 100% biocidal efficiency against E. coli (5 * 104E. coli/mg particles) during ten recycling procedures without any treatment. More importantly, according to the results of trapping behavior and antibiofilm assays, not only bacteria could be captured by the particles (trapping rate was over 85%), but also the residual debris from dead bacteria and fragmented biofilm was together removed based on the special structure and functions of the antibacterial particles (~ 80%), including extremely rough surfaces, surficial positive charge and magneto-responsive property. This study presents an efficient approach for microorganism management in water system which can be expectantly applied to improve the water safety

Ultrafast laser direct writing of screen-printed graphene-based strain electrodes for sensing glass deformation

This study aimed to develop screen-printed graphene-based strain sensors for sensing the deformation of glass. Graphene electrodes of strain sensors with various grid lengths were fabricated by ultrafast laser direct writing. The experimental results revealed that the laser ablation threshold for graphene films was approximately 0.35 J/cm2. For areal fluences exceeding 7.8 J/cm2, the laser-ablated zone on graphene films served as electrical insulation because the conductive film was completely removed. The optimal laser fabrication parameters for strain sensors were a pulse repetition frequency of 300 kHz, areal fluence of 31.9 J/cm2, scan speed of 500 mm/s, line-scan spacing of 0.01 mm, and overlapping rate of laser spots of 94.4%. Laser-formed electrode structures on graphene films had straight edges and sharp corners. Moreover, no residual film and debris existed on the processing paths. During deformation tests on glass substrates with bending distances of 0–180 μm, the strain sensor with a 6-mm grid length exhibited an excellent linear relationship with the change in resistance. Furthermore, the gauge factor of 550.14 for strain sensors with a 6-mm grid length was larger than that of 392.48 and 308.17 for strain sensors with 8- and 10-mm grid lengths, respectively.

Research on the machining characteristics of vertical ultrasonic vibration assisted WEDM-LS

ABSTRACT In this paper, a novel composite process technology – Low Speed Wire Electrical Discharge Machining (WEDM-LS) with Vertical Ultrasonic Vibration (VUV) assisted is put forward to improve the performance of traditional WEDM-LS. First, a fluid-solid coupling model of interelectrode flow field and workpiece is established. The simulation result reveals that under the assistance of Vertical Ultrasonic Vibration of workpiece, the velocity of deionized water near the wall of discharge craters is much higher than traditional WEDM-LS or WEDM-LS with the assistance of wire ultrasonic vibration (Wire USV). Therefore, Vertical Ultrasonic Vibration is helpful to the removal of residual debris from the craters. Second, the multiphase flow model of debris, deionized water, and bubbles between electrodes was constituted, and it shows that workpiece vibration frequency is positively correlated with the movement intensity of bubbles and debris, which promotes debris removal throughout the whole processing area. Finally, based on the Taguchi method, the experiments of traditional WEDM-LS, Wire USV assisted WEDM-LS and VUV assisted WEDM-LS were conducted. The workpiece was selected as TiNi-01 shape memory alloy (SMA). The experimental results reveal that under the large electrical machining parameters, Vertical Ultrasonic Vibration assisted WEDM-LS can achieve excellent machining efficiency and quality.

Ultrastructural Analysis of Volumetric Histotripsy Bio-effects in Large Human Hematomas

Large-volume soft tissue hematomas are a serious clinical problem, which, if untreated, can have severe consequences. Current treatments are associated with significant pain and discomfort. It has been reported that in an in vitro bovine hematoma model, pulsed high-intensity focused ultrasound (HIFU) ablation, termed histotripsy, can be used to rapidly and non-invasively liquefy the hematoma through localized bubble activity, enabling fine-needle aspiration. The goals of this study were to evaluate the efficiency and speed of volumetric histotripsy liquefaction using a large in vitro human hematoma model. Large human hematoma phantoms (85 cc) were formed by recalcifying blood anticoagulated with citrate phosphate dextrose/saline–adenine–glucose–mannitol solution. Typical boiling histotripsy pulses (10 or 2 ms) or hybrid histotripsy pulses using higher-amplitude and shorter pulses (0.4 ms) were delivered at 1% duty cycle while continuously translating the HIFU focus location. Histotripsy exposures were performed under ultrasound guidance with a 1.5-MHz transducer (8-cm aperture, F# = 0.75). The volume of liquefied lesions was determined by ultrasound imaging and gross inspection. Untreated hematoma samples and samples of the liquefied lesions aspirated using a fine needle were analyzed cytologically and ultrastructurally with scanning electron microscopy. All exposures resulted in uniform liquid-filled voids with sharp edges; liquefaction speed was higher for exposures with shorter pulses and higher shock amplitudes at the focus (up to 0.32, 0.68 and 2.62 mL/min for 10-, 2- and 0.4-ms pulses, respectively). Cytological and ultrastructural observations revealed completely homogenized blood cells and fibrin fragments in the lysate. Most of the fibrin fragments were less than 20 μm in length, but a number of fragments were up to 150 μm. The lysate with residual debris of that size would potentially be amenable to fine-needle aspiration without risk for needle clogging in clinical implementation.

Efficacy of Handpiece Mounted Continuous Irrigation System for the Removal of Residual Pulpal Debris During Root Canal Preparation.

Objective The removal of residual pulpal debris (RPD) from the root canal walls, especially in necrotic teeth is important for successful endodontic outcome. The aim of this study was to evaluate the RPD removal efficacy of handpiece mounted continuous irrigation with simultaneous root canal preparation from the apical third of root canals as compared to conventional syringe irrigation (SI). Materials and Methods Ninety extracted teeth were randomly divided into three groups: SI; handpiece mounted continuous irrigation (CI); and both syringe and continuous irrigation (CI+SI). After root canal preparation, roots were sectioned at 1 (a), 3 (b) and 5 (c) mm from apex and prepared for Hematoxylin-Eosin staining. Sections were microscopically examined for presence of RPD. Statistical Analysis Intergroup difference in average percentage of RPD at different root levels was calculated by using analysis of variance test (ANOVA). Tuckey test was used for pairwise comparison. Results ANOVA showed significant difference between all three groups ( p < 0.05). SI group showed a significantly higher percentage of RPD as compared with CI and CI+ SI groups at all root levels ( p < 0.01). At root level a, CI showed a significantly higher percentage of RPD as compared with CI+SI ( p < 0.01). Conclusion Handpiece mounted CI during rotary instrumentation showed a significant reduction in RPD as compared with conventional syringe irrigation. This technique was found to be an effective method for an enhanced root canal debridement.

Corrosion Behaviour of Bamboo Leaf Ash-Reinforced Nickel Surface-Deposited Aluminium Metal Matrix Composites

The global consumption of bamboo leaves behind with greater amounts of waste leaves occupies a majority of landfills. Burning of this residual debris (bamboo leaves) in an unrestrained manner poses out multiple adverse and irreversible effects on both human and the environment we live in. A large number of residual wastes are being generated through industrial and agricultural operations due to the forever increasing world population. These residual debris is often involving much complicated processes for disposal. Converting the stubborn residual debris to green material systems is regarded as optimal and effective way for the current pollution crisis. The current study mainly intends for the use of calcined ash from bamboo waste leaves which have a tremendous pozzolanic properties and can be used as natural industrial-agro filler and inspecting the corrosion behaviour of nickel-deposited aluminium–bamboo leaf ash composites. The base A356.2 aluminium alloy is reinforced with bamboo leaf ash particulates by employing stir casting technique varying the reinforcement percentages, i.e. 2, 4 and 6 wt. %. The fabricated Al-BLA composites are nickel electrodeposited with a customary watt’s bath. The corrosion behaviour of the test samples is investigated thoroughly by employing polarization tests under aerated 3.5% Nacl ambience. The microstructural and coating surface morphology is studied by employing SEM and XRD practices. It was noted the nickel-deposited Al-BLA composites flaunted enhanced corrosion resistance and elevated mechanical behaviour upon increasing the percentage of the reinforcement.

The effect of temporary sealing materials and cleaning protocols on the bond strength of resin cement applied to dentin using the resin-coating technique.

This study evaluated the effect of temporary sealing materials and cleaning protocols on the bond strength of resin cement applied to dentin using resin-coating technique. Scotchbond Universal Adhesive and Filtek Supreme Ultra Flowable were applied to bovine dentin. Forty-five specimens were divided into the following three groups according to the temporary sealing materials: Cav-: CAVITON EX, Vas-: COCOA BUTTER and FIT SEAL, and Sep-: Washable SEP and FIT SEAL. Each material was placed on resin-coated dentin. After 1-week water storage, one of the following three cleaning protocols was performed: -WA: washed with water, -BR: brushed with PRESSAGE, and -AF: cleaned with AIR-FLOW. Microtensile bond strength test and EDS analysis were conducted. Irrespective of the cleaning protocol used, Washable SEP demonstrated less residual debris on resin-coated dentin, resulting in high bond strength. Regardless of the temporary sealing material applied, AIR-FLOW demonstrated less residual debris, resulting in high bond strength.

Optimization of extracellular matrix extraction from human perirenal adipose tissue

Human adipose tissue includes useful substrates for regenerative medicine such as the extracellular matrix (ECM), but most perirenal fat tissue is wasted after kidney surgery. Since a lot of adipose tissue can be procured after a kidney, we extracted ECM from human perirenal adipose tissue and optimized the extraction process. To verify the efficacy for ECM extraction, we compared the products in several steps. Perirenal adipose tissue was either finely homogenized or underwent crude manual dissection. The amount of extracted ECM was quantified with ELISA for verification of the initial tissue downsizing effect. To validate the drying effect for fast and complete delipidation, tissues were prepared in a dry or wet phase, and residual lipids were visualized with Oil-Red-O staining. The extracted lipid was assayed at each time point to quantify the appropriate delipidation time. To select the optimal decellularization method, tissues were treated with physical, chemical, or enzymatic method, and the residual cell debris were identified with histological staining. The biochemical properties of the ECM extracted by the above methods were analyzed. The ECM extracted by fine homogenization showed a significantly enhanced amount of collagen, laminin and fibronectin compared to the crude dissection method. The dried tissue showed fast and complete lipid elimination compared to the wet tissue. Complete delipidation was achieved at 45 min after acetone treatment. Additionally, 1% triton X-100 chemical treatment showed complete decellularization with well-preserved collagen fibers. Biochemical analysis revealed preserved ECM proteins, a high cell proliferation rate and normal cell morphology without cell debris or lipids. The established process of homogenization, drying, delipidation with acetone, and decellularization with Triton X-100 treatment can be an optimal method for ECM extraction from human perirenal adipose tissue. Using this technique, human perirenal adipose tissue may be a valuable source for tissue engineering and regenerative medicine.

Water-assisted femtosecond laser drilling of alumina ceramics

In this study, we used a water-assisted femtosecond laser drilling method to drill holes in alumina ceramics. The morphology, diameter, taper angle, cross-section area, and sidewall characteristics of the holes were investigated, and the hole characteristics obtained in the presence of air were compared with those obtained under water assistance. Finally, the mechanism underlying the water-assisted femtosecond laser drilling of alumina ceramics was investigated. The results revealed that the drilling efficiency and quality of the holes improved significantly by using the water-assisted femtosecond laser drilling method, and the effect was considerably obvious at lower pulse repetition rates. At the pulse repetition rate of 25 kHz, the taper angle decreased by 64.19% to 4.20° and the hole cross-section area increased by 46% under water assistance. In addition, water assistance could reduce the amount of the residual debris and redeposition of ablated material on the hole sidewalls, and there were almost no residual debris and redeposition of ablated material on the hole sidewalls near the hole exit. Compared to the case when the drilling was carried out in the presence of air, the composition of the hole sidewalls changed slightly when the drilling was carried out in the presence of water. With an increase in the pulse repetition rate, more plasma was formed and the plasma shielding effect strengthened, which weakened the effect of water assistance. Therefore, in order to obtain high drilling efficiency and quality during the water-assisted femtosecond laser drilling of alumina ceramics, it is necessary to use a low pulse repetition rate. The results of this study will find potential application in the field of ceramic manufacturing.

Juvenile, but Not Adult, Mice Display Increased Myeloid Recruitment and Extracellular Matrix Remodeling during Respiratory Syncytial Virus Infection.

Early life respiratory syncytial virus (RSV) infection has been linked to the onset of asthma. Despite this association, our knowledge of the progression of the initial viral infection is limited, and no safe or effective vaccine currently exists. Bronchioalveolar lavage, whole-lung cellular isolation, and gene expression analysis were performed on 3-wk- (juvenile) and 8-wk-old (adult) RSV-infected C57BL/6 mice to investigate age-related differences in immunologic responses; juvenile mice displayed a sustained myeloid infiltrate (including monocytes and neutrophils) with increased RNA expression of Ccl2, Ccl3, and Ccl4, when compared with adult mice, at 72 h postinfection. Juvenile mice demonstrated αSma expression (indicative of myofibroblast activity), increased hyaluronan deposition in the lung parenchyma (attributed to asthma progression), and a lack of CD64 upregulation on the surface of monocytes (which, in conjunction with serum amyloid P, is responsible for clearing residual hyaluronan and cellular debris). RSV infection of human airway epithelial cell, human lung fibroblast, and U937 monocyte cocultures (at air-liquid interface) displayed similar CCL expression and suggested matrix metalloproteinase-7 and MMP9 as possible extracellular matrix modifiers. These mouse data, in conjunction with our findings in human monocytes, suggest that the sustained influx of myeloid cells in the lungs of juvenile mice during acute RSV infection could potentiate extracellular matrix remodeling, facilitating conditions that support the development of asthma.

Distinct fungal plastisphere across different river functional zones: A watershed scale study

Fungi not only play important roles in biogeochemical processes but also can form biofilm on plastic debris. However, knowledge of structure composition and spatiotemporal pattern of fungal plastisphere on different kinds of plastic debris in river with specific usages, known as river functional zones, is still missing. In this study, we investigated the spatial distribution of the fungal plastisphere across a complete urban river with different functional zones (drinking, farm irrigation, aquaculture, and tail lake). Our research was performed based on both field residual plastic debris collection and a 30-day field in situ incubation experiments. Our study revealed that plastic debris enriched distinct fungal communities (including pathogenic fungi) significantly different from the surrounding water. Tracking the source of the fungi colonized on plastic debris suggested that the fungal taxa colonized on the different kinds of plastic debris were not from the surrounding water. Human activities had considerable effects on the fungal community structure on plastic debris, and the plastisphere fungal community structure strikingly varied across different river functional zones. Plastisphere may be used as an indicator for fungi biogeography and pathogenic fungi pollution in river with different functional zones. These findings are essential for ecological risk assessment and management decisions for pollution control of plastic debris and maintaining ecological health.

In vitro evaluation of ultrafast laser drilling large-size holes on sheepshank bone

Bone drilling has been widely used in medical surgeries such as repair and fixation in orthopedics. Traditional drilling method using drill-bits inevitably causes significant thermal and mechanical trauma in the adjacent bone tissues. This paper demonstrates the feasibility of femtosecond laser drilling in vitro large-size holes on the sheepshank bone with high efficiency and minimal collateral damage. A Yb:KGW femtosecond laser was utilized to drill millimeter-scale holes on the bone under different cooling conditions including gas- and water-assisted processes. Scanning electron microscopy, confocal laser scanning microscopy and infrared thermographic imaging system were used to investigate the residual debris, removal rate, bone temperature variation and hole morphology. Histological examination, Fourier transform infrared spectroscopy and Raman spectroscopy were employed to study thermal damage. Results show that a 4 mm hole with smooth and clean surface was successfully drilled on the bone, and the highest removal rate of 0.99 mm3/s was achieved, which was twenty times higher than the previous study of 0.05 mm3/s. Moreover, bone and bone marrow were distinguished by real-time monitoring system during laser drilling. This work demonstrates the potential for clinical applications using an ultrafast laser to produce crack-free large-size bone holes.

Ultrafiltration and microfiltration membrane performance, cleaning, and flux recovery for microalgal harvesting

Membrane material, pore size, and cleaning strategy are important factors for microalgal membrane harvesting. In this study, harvesting of Scenedesmus acuminatus cultivated in pilot scale using nine ultrafiltration and three microfiltration membranes was carried out in cross-flow filtration system to compare their filtration performance and flux recovery after physical and chemical cleaning. The 0.45-μm PVDF-AsahiKASEI membrane had the highest average flux of 513.6 L m−2 h−1 among 12 membranes and 50-kDa PVC-Litree membrane had the highest average flux of 98.0 L m−2 h−1 among nine ultrafiltration membranes for 60 L S. acuminatus suspension harvesting. There were significantly positive correlations between membrane pore size, pure water flux, and average flux for S. acuminatus suspension harvesting. Generally, ultrafiltration membrane had moderate total fouling index (TFI) and high proportion of hydraulic reversible fouling index (HRFI) of more than 95%. The 0.45-μm PVDF-AsahiKASEI membrane had a low TFI but the lowest proportion of HRFI, associated with the residual microalgal cells, debris, and colloids on the inner surface and top ends of some cells inserted to big membrane pores, demonstrated by SEM image and reduced porosity from 65.9 to 60.2%. Compared to soaking cleaning, circulation cleaning strategy had a better flux recovery efficiency for 0.45-μm PVDF-AsahiKASEI membrane, proving that it would be a potential way in membrane chemical cleaning. This study demonstrated that microfiltration with high flux has great potential in microalgal harvesting if flux recovery efficiency can be significantly increased using suitable cleaning strategy such as circulation cleaning strategy.