To study the urodynamic characteristics of women with pelvic organ prolapse in prolapse and reset condition. From Jan. 2010 to Apr. 2010, 30 patients with pelvic organ prolapse (POP), including 1 case in grade I, 6 cases in grade II, 21 cases in grade III and 2 cases in grade IV, treated in Peking University People's Hospital were analyzed their urodynamical changes at status of prolapse and reset with pessary. (1) The symptoms: in the prolapse condition, there were 11 patients with bladder outlet obstruction (BOO), 7 patients with residual volume more than 100 ml, and 4 patients with stress urinary incontinence (SUI). In the reset condition, there were 5 patients with BOO, 5 patients with residual volume more than 100 ml, and 4 SUI patients. (2) Characteristics of urodynamic: there were significant difference in first desire to void (FD, P = 0.047), normal desire to void (ND, P = 0.007), strong desire to void (SD, P = 0.001), maximum cystometric capacity (MCC, P = 0.001), maximum flow rate (Q(max), P = 0.001), average flow rate (Q(ave), P = 0.001), and residual volume (RV, P = 0.025) between prolapse and reset condition among all patients. In patients with grade I to II prolapse, Q(max) were (11 ± 6) ml/s in prolapse condition and (15 ± 4) ml/s in reset condition (P = 0.014), Q(ave) were (6 ± 4) ml/s in prolapse condition and (7 ± 3) ml/s in reset condition (P = 0.237), RV were (29 ± 46) ml in prolapse condition and (15 ± 25) ml in reset condition (P = 0.157). Among patients with grade III to IV prolapse, Q(max) were (11 ± 8) ml/s in prolapse condition and (17 ± 10) ml/s in reset condition (P = 0.001), Q(ave) were (5 ± 4) ml/s in prolapse condition and (7 ± 4) ml/s in reset condition (P = 0.002), RV were (105 ± 169) ml in prolapse condition and (47 ± 92) ml in reset condition (P = 0.037). Patients with pelvic organ prolapse III-IV may present pseudo BOO, recessive stress urinary incontinence and residual volume more than 100 ml. There were significant improvement in cystometric sensation, capacity, flow rate, and residual volume after reset. The urodynamic test for patients with pelvic organ prolapse in the reset condition might be much better in evaluating lower urinary tract function.