Reservoir Of Antibiotic Resistant Bacteria Research Articles

What Antibiotic Threat Do the Heavy Metals Contaminated Sites of Mine Hide?

The environment contaminated by antibiotics and heavy metals as a consequence of human activities is of great concern nowadays.Many pieces of research proved that the environment could act as a reservoir of antibiotic resistance determinants allowing themto spread among different bacterial species via the process called horizontal gene transfer. The result is antibiotic resistance even inpathogen microorganisms. Heavy metals act as important factors in this process because of their potential to select antibiotic resistantbacteria thanks to linkage among antibiotic resistance genes and heavy metals resistance genes.Thus, this experiment was conducted to screen the antibiotic tolerance profile of bacteria obtained from heavy metal contaminatedenvironment of mine, dump and the contaminated soil near the entry of mine.Several samples were collected from the only active gold mine in Slovakia in Hodruša – Hámre. The presence of cultivable bacteria wasproved via cultivation approaches with subsequent MALDI – TOF MS (Matrix – Assisted Laser Desorption/Ionisation Time of FlightMass Spectrometry) identification of selected isolates. Representative bacterial isolates were screened for their antibiotic toleranceagainst chosen antibiotics (ampicillin (AMP), chloramphenicol (CHLOR), tetracycline (TET) and kanamycine (KAN)) with the aimto define their minimal inhibitory concentration (MIC).The cultivable bacteria from studied environments were dominated by Gram-negative protebacteria of Pseudomonas and Rhizobiumgenera. Among more than 150 isolates the resistance to ampicillin (MIC>100µg/ml – 49% isolates), kanamycine (MIC>100µg/ml -18% isolates), and chloramphenicol (MIC>20µg/ml – 16% isolates) dominated. The resistance to tetracycline (MIC>20µg/ml) wasdetected in less than 1% of isolates. Overall counts of antibiotic resistance and multi-resistance were alarmingly high taking in accountthat industrial environments with no known antibiotic exposure were analysed.Our data indicate that heavy metals contaminated environment could influence the occurrence and the spread of antibiotic resistance.Possibly, metal contaminated environment act as a reservoir of antibiotic resistant bacteria.

Contribution of the Antropically – Impacted Aquatic Ecosystems to the Resistance Reservior

Aquatic ecosystems are reservoirs of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), having a major role in their occurrence, accumulation and dissemination. The purpose of this review is to highlight the influence of wastewater treatment plants (WWTP) effluents, manure and biosolids use in agriculture as well as of aquaculture upon the development of antibiotic resistance (AR). Research indicates the need to streamline treatment strategies in order to minimize the risk of AR spread in the aquatic environment through wastewater.

Metagenomic characterization of bacterial community and antibiotic resistance genes in representative ready-to-eat food in southern China

Ready-to-eat (RTE) foods have been considered to be reservoirs of antibiotic resistance bacteria, which constitute direct threat to human health, but the potential microbiological risks of RTE foods remain largely unexplored. In this study, the metagenomic approach was employed to characterize the comprehensive profiles of bacterial community and antibiotic resistance gene (ARG) in 18 RTE food samples (8 RTE meat, 7 RTE vegetables and 3 RTE fruit) in southern China. In total, the most abundant phyla in RTE foods were Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidetes and Actinobacteria. 204 ARG subtypes belonging to 18 ARG types were detected with an abundance range between 2.81 × 10−5 and 7.7 × 10−1 copy of ARG per copy of 16S rRNA gene. Multidrug-resistant genes were the most predominant ARG type in the RTE foods. Chloramphenicol, macrolide-lincosamide-streptogramin, multidrug resistance, aminoglycoside, bacitracin, tetracycline and β-lactam resistance genes were dominant, which were also associated with antibiotics used extensively in human medicine or veterinary medicine/promoters. Variation partitioning analysis indicated that the join effect of bacterial community and mobile genetic elements (MGEs) played an important role in the resistome alteration. This study further deepens the comprehensive understanding of antibiotic resistome and the correlations among the antibiotic resistome, microbiota, and MGEs in the RTE foods.

Fate and seasonal change of Escherichia coli resistant to different antibiotic classes at each stage of conventional activated sludge process.

This study investigated the impact of each treatment stage of the activated sludge process on the fate of antibiotic resistant bacteria (ARB) in wastewater treatment plants (WWTPs). Wastewater and sludge samples were collected monthly at each stage of a commercial-scale WWTP. After 20-25 strains of indicator Escherichia coli were isolated from each sample on Chromocult Coliform Agar, antibiotic resistance of the isolates to amoxicillin (AMX), ciprofloxacin (CIP), norfloxacin (NFX), kanamycin (KM), sulfamethoxazole/trimethoprim (ST) and tetracycline (TC) were tested with the Kirby-Bauer disk diffusion method. As a result, activated sludge in the aeration tank and return sludge had higher abundance of antibiotic resistant E. coli than influent wastewater and secondary treatment effluent. AMX resistant E. coli was enriched in return sludge at the secondary clarifier. Higher temperature was also likely to cause an increase of AMX resistant E. coli in sludge. The antibiotic resistance profile of E. coli in secondary treatment effluent was more dependent on activated sludge than influent wastewater. These results suggested that activated sludge in WWTP possibly serves as a reservoir of ARB, and that behavior of ARB in WWTP differs by antibiotic classes.

Occurrence of antibiotic resistance genes and microbial source tracking markers in the water of a karst spring in Germany

The emergence of antimicrobial resistances causes serious public health concerns worldwide. In recent years, the aquatic ecosystem has been recognized as a reservoir for antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). The prevalence of 11 ARGs, active against six antibiotic classes (β-lactams, aminoglycosides, tetracycline, macrolides, trimethoprim, and sulfonamides), was evaluated at a karst spring (Gallusquelle) in Germany, using molecular biological methods. In addition, fecal indicator bacteria (FIB), turbidity, electrical conductivity, spring discharge, and microbial source tracking markers specific for human, horse, chicken, and cow were determined. The ARGs most frequently detected were ermB (42.1%), tet(C) (40.8%), sul2 (39.5%), and sul1 (36.8%), which code for resistance to macrolides, tetracycline and sulfonamides, respectively. After a heavy rain event, the increase in FIB in the spring water was associated with the increase in ARGs and human-specific microbial source tracking (MST) markers. The determined correlations of the microbiological parameters, the observed overflow of a combined sewer overflow basin a few days before the increase of these parameters, and the findings of previous studies indicate that the overflow of this undersized basin located 9 km away from the spring could be a factor affecting the water quality of the karst spring. Our results provide a scientific basis for minimization of the input of fecal pollution and thus ARGs into the karst spring.

Faecal microbiota transplant to ERadicate gastrointestinal carriage of Antibiotic Resistant Organisms (FERARO): a prospective, randomised placebo-controlled feasibility trial

IntroductionAntimicrobial resistance is rising, largely due to the indiscriminate use of antimicrobials. The human gut is the largest reservoir of antibiotic resistant bacteria (ARB). Individuals colonised with ARB have the...

Differences in the Microbial Community and Resistome Structures of Feces from Preweaned Calves and Lactating Dairy Cows in Commercial Dairy Herds.

Preweaned dairy calves and lactating dairy cows are known reservoirs of antibiotic-resistant bacteria. To further understand the differences in the resistomes and microbial communities between the two, we sequenced the metagenomes of fecal composite samples from preweaned dairy calves and lactating dairy cows on 17 commercial dairy farms (n = 34 samples). Results indicated significant differences in the structures of the microbial communities (analysis of similarities [ANOSIM] R = 0.81, p = 0.001) and resistomes (ANOSIM R = 0.93 to 0.96, p = 0.001) between the two age groups. Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria were the predominant members of the communities, but when the groups were compared, Bacteroidetes and Verrumicrobia were significantly more abundant in calf fecal composite samples, whereas Firmicutes, Spirochaetes, Deinococcus-Thermus, Lentisphaerae, Planctomycetes, Chlorofexi, and Saccharibacteria-(TM7) were more abundant in lactating cow samples. Diverse suites of antibiotic resistance genes (ARGs) were identified in all samples, with the most frequently detected being assigned to tetracycline and aminoglycoside resistance. When the two groups were compared, ARGs were significantly more abundant in composite fecal samples from calves than those from lactating cows (calf median ARG abundance = 1.8 × 100 ARG/16S ribosomal RNA [rRNA], cow median ARG abundance = 1.7 × 10-1 ARG/16S rRNA) and at the antibiotic resistance class level, the relative abundance of tetracycline, trimethoprim, aminoglycoside, macrolide-lincosamide-streptogramin B, β-lactam, and phenicol resistance genes was significantly higher in calf samples than in cow samples. Results of this study indicate that composite feces from preweaned calves harbor different bacterial communities and resistomes than composite feces from lactating cows, with a greater abundance of resistance genes detected in preweaned calf feces.

Distribution characteristics of antibiotic resistant bacteria and genes in fresh and composted manures of livestock farms

Livestock manure is a major reservoir of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). This study investigated the distribution characteristics of ARB, ARGs in fresh and composted manures of traditional breading industry in rural areas in China. Samples collected were naturally piled without professional composting, and will be applied to farmland. The real-time quantitative polymerase chain reaction (qPCR) results showed the presence of ten target ARGs and two mobile genetic elements (MGEs) in the tested manure samples. The relative abundance of tetracycline and sulfonamide resistance genes (TRGs and SRGs) was generally higher than that of macrolide resistance genes (MRGs), followed by quinolone resistance genes (QRGs). There were significant positive correlations between the abundance of sul1, sul2, tetW and MGEs (intl1, intl2). In addition, the distribution of target ARGs was associated with the residual concentrations of doxycycline (DOX), sulfamethazine (SM2), enrofloxacin (ENR) and tylosin (TYL). Overall, a total of 24 bacterial genera were identified. The resistance rates of ARB were 17.79%–83.70% for SM2, followed 0.40%–63.77% for TYL, 0.36%–43.90% for DOX and 0.00%–13.36% for ENR, which showed a significant dose-effect. This study also demonstrated that the abundance of clinically relevant ARB and ARGs in chicken, swine and cow fresh manures significantly greater than that in composted manures, and chicken and swine manures had higher proportion of ARB and higher abundance of ARGs than that in cow manures.

Class 1 Integron, Sulfonamide and Florfenicol Resistance Genes in Bacteria from Three Unsanitary Landfills, Ibadan, Nigeria

Objective: Municipal solid waste (MSW) landfills recently emerged as potential reservoirs of antibiotic resistance. However, little is known about the potentials of unsanitary landfill sites which are common in developing countries as reservoir of antibiotic resistance. In this study, we investigated the antibiotic resistance pattern and presence of selected resistance genes in bacteria from three MSW landfills in Ibadan, southwestern Nigeria. Methodology: Fifty six antibiotic- and metal-resistant bacteria isolated from leachate and leachate-contaminated surface and groundwater collected from three MSW landfills in Ibadan, Southwestern Nigeria, were searched for sulfonamides, florfenicol and beta-lactams resistance genes and integrons by PCR. Results: None of the 27 β-lactamase genes tested was detected in the isolates. sul1 , sul2 and floR were however detected in 12 (21.4%), 2 (3.6%) and 12 (21.4%) bacteria species identified as Alcaligenes faecalis , Advenella kashmirensis , Brevundimonas spp. and Ralstonia pickettii . The 12 bacteria carrying sul1 / floR gene combination were also positive for class 1 integron. Analysis of the gene cassettes in the variable regions of the class 1 integrons (>1.5kb) of the 12 intl1-positive isolates revealed the presence of aadB and unknown genes. The deduced amino acid sequence of the unknown portion of the gene cassettes shared 42-65% identity with sequences of Class D β-lactamase and OXA-2-like protein in the GenBank. Conclusions: Findings of this study suggest that the landfill ecosystem is a potential site for the evolution of novel resistance genes and are hence important reservoir of antibiotic resistant bacteria. J Microbiol Infect Dis 2019; 9(1): 34-42.

Detection of plasmid-mediated quinolone resistance genes in β-lactamase-producing Escherichia coliisolates from layer hens

This study was conducted to investigate the presence of plasmid-mediated quinolone resistance (PMQR) genes in β-lactamase-producing Escherichia coli isolates from layer hens and to characterize their molecular background. Among 142 E. coli isolates, 86 (60.6%) showed multidrug resistance and 15 (10.6%) were found to be β-lactamase-producing E. coli. Extended-spectrum β-lactamase (ESBL) and plasmid-mediated AmpC (pAmpC) β-lactamase genes, blaCTX-M-14 and blaCMY-2, were identified in three and six E. coli isolates, respectively. The non-ESBL or pAmpC gene, blaTEM-1, was found in eight of the isolates. Two isolates had both genes, blaCTX-M-14 and blaTEM-1. Among the 15 β-lactamase-producing E. coli, six PMQR genes, qnrS1 (n = 3) and qnrB4 (n = 3), were identified. Among the six PMQR-positive E. coli isolates, four exhibited double amino acid exchanges at both gyrA and parC with ciprofloxacin and enrofloxacin minimum inhibitory concentrations of ≥32 and ≥16μg/mL, respectively. Additionally, five transconjugants (33.3%) showed a transferability of β-lactamase and PMQR genes. Pulsed-field gel electrophoresis (PFGE) analysis was conducted to investigate the 15 β-lactamase-producing E. coli isolates. In PFGE, E. coli included three PFGE patterns showing the same farms and in accordance with both β-lactamase and PMQR genes and the antimicrobial resistance pattern. Layer hens may act as a reservoir of antibiotic-resistant bacteria, and the PMQR gene in β-lactamase-producing E. coli isolates from layer hens has the potential to enter the food chain. Therefore, our findings suggest that comprehensive surveillance of antimicrobial use in laying operation systems is necessary.

Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn

Wastewater treatment plants (WWTPs) and municipal waste management plants (MWMPs) emit bioaerosols containing potentially pathogenic biological components which post a threat for human health. Microbiological monitoring supports evaluations of the antibiotic resistance (AR) of airborne microorganisms and the relevant health risks. The aim of this study was to analyze the microbiological quality of air sampled in a WWTP and MWMP in Olsztyn based on total bacterial counts, the presence of bacteria resistant to three antibiotic classes (beta-lactams, tetracyclines and chloramphenicol) and genes encoding resistance to these antibiotics (blaTEM, blaSHV, blaCMY-2, blaAmpC, tet(M), tet(A), tet(X), tet(B), cmlA, floR, fexA, fexB and catA1 ). Bacterial counts were higher in air samples collected from the MWMP (~104 CFU/m3) than from the WWTP (101–103 CFU/m3). A similar trend was noted in the counts of antibiotic resistant bacteria (ARB). The abundance of ARB did not exceed 1.7 x 102 CFU/m3 in WWTP samples, but was higher at up to 4.2 x 103 CFU/m3 in MWMP samples. Bacteria resistant to doxycycline were least prevalent in the analyzed ARB. In the group of 49 tested bacterial strains, 44 harbored at least one of the analyzed antibiotic resistance genes (ARGs). A comparison of ARGs in all bacterial strains isolated from WWTP and MWMP air samples revealed the highest diversity and prevalence of ARGs in the samples collected in the mechanical segment of the waste processing line in MWMP and the biological segment of the wastewater processing line in WWTP. The results of this study point to high microbiological contamination of air in MWMPs and WWTPs which are reservoirs of ARB and ARGs and potential sources of AR.

Current Mechanism of Bacterial Resistance to Antimicrobials

Serious infectious diseases are caused by bacterial pathogens that represents a serious public health concern. Antimicrobial agents are indicated for the treatment bacterial infections.Various bacteria carries several resistance genes also called multidrug resistant (MDR). Multidrug resistant organisms have emerged not only in the hospital environment but are now often identified in community settings, suggesting the reservoirs of antibiotic resistant bacteria are present outside the hospital. Drug resistant bacteria that are selected with a single drug are also frequently multi-drug resistant against multiple structurally different drugs, thus confounding the chemotherapeutic efficacy of infectious disease caused by such pathogenic variants. The molecular mechanisms by which bacteria have common resistance to antibiotics are diverse and complex. This review highlights the mechanism of bacterial resistance to antimicrobials.

Long-term carriage and rapid transmission of extended spectrum beta-lactamase-producing E. coli within a flock of Mallards in the absence of antibiotic selection.

Wild birds have been suggested as transmitters and reservoirs for antibiotic resistant bacteria. We performed an experimental study investigating carriage time and interindividual transmission of extended spectrum beta-lactamase- (ESBL-)producing Escherichia coli in Mallards (Anas platyrhynchos) to assess if the birds carry the bacteria long enough to transfer them geographically during migration. Mallards were inoculated intraoesophageally with four different strains of ESBL-producing E. coli and kept together in a flock. The ESBL-strains belonged to sequence types previously shown to spread between birds and humans. Culturing from faecal samples showed presence of ESBL-producing E. coli the entire 29 day experimental period. An extensive and rapid transmission of the different ESBL-strains between individuals (including non-inoculated controls) was observed. In necropsy samples, we detected ESBL-strains in the cecum even in faeces-negative birds, indicating that this part of the intestine could function as a reservoir of resistant bacteria. We demonstrate that birds can carry ESBL-producing E. coli for long enough times to travel far during migration and the extensive interindividual transmission suggests spread between individuals in a dense bird population as a mechanism that allow persistence of resistant bacteria.

Current Mechanism of Bacterial Resistance to Antimicrobials

Serious infectious diseases are caused by bacterial pathogens that represents a serious public health concern. Antimicrobial agents are indicated for the treatment bacterial infections.Various bacteria carries several resistance genes also called multidrug resistant (MDR). Multidrug resistant organisms have emerged not only in the hospital environment but are now often identified in community settings, suggesting the reservoirs of antibiotic resistant bacteria are present outside the hospital. Drug resistant bacteria that are selected with a single drug are also frequently multi-drug resistant against multiple structurally different drugs, thus confounding the chemotherapeutic efficacy of infectious disease caused by such pathogenic variants. The molecular mechanisms by which bacteria have common resistance to antibiotics are diverse and complex. This review highlights the mechanism of bacterial resistance to antimicrobials.

Antibiotic resistant bacteria and resistance genes in the bottom sediment of a small stream and the potential impact of remobilization.

River sediments are regarded as hot spots of bacterial density and activity. Moreover, high bacterial densities and biofilm formation are known to promote horizontal gene transfer, the latter playing a vital role in the spread of antimicrobial resistance. It can thus be hypothesized that sediments act as a reservoir of antibiotic resistant bacteria (ARB) and resistance genes (ARGs), particularly in rivers receiving microbes and drug residues from treated sewage. We analyzed the phenotypic susceptibility of 782 Escherichia coli isolates against 24 antimicrobials and we measured the relative abundances of five ARGs in water and sediment extracts of a small stream. We did not find evidence for a general increase in the proportion of resistant E. coli isolated from sediments as compared to those found in stream water. For most antimicrobials, the likelihood of detecting a resistant isolate was similar in water and sediment or it was even lower in the latter compartment. The mean relative abundance of ARGs was moderately increased in sediment-borne samples. Generally, absolute abundances of resistant cells and resistance genes in the sediment exceeded the pelagic level owing to higher bacterial densities. The river bottom thus represents a reservoir of ARB and ARGs that can be mobilized by resuspension.

The prevalence of multidrug-resistant Aeromonas spp. in the municipal wastewater system and their dissemination in the environment

The objective of this study was to identify the determinants of antibiotic resistance and virulence of Aeromonas spp. isolated from treated wastewater (WW) and from samples of river water collected upstream (URW) and downstream (DRW) from the effluent discharge point. The resistance of Aeromonas spp. to antibiotics that are widely used in human and veterinary medicine, including beta-lactams, tetracyclines, glycylcyclines, fluoroquinolones, aminoglycosides and sulfamethoxazole-trimethoprim, was analyzed by disk diffusion method. The prevalence of hemolysins, aerolysins (virulence factors) and integrase genes was determined. A total of 83 Aeromonas spp. strains were selected from the isolates obtained from river water and wastewater samples. The highest percentage (81.8%) of multidrug-resistant isolates was noted in DRW samples. The analyzed isolates were most frequently resistant to beta-lactams, tetracyclines and aminoglycosides, whereas resistance to glycylcyclines was least common. Isolates resistant to beta-lactams most frequently harbored blaTEM and blaOXA genes. The group of genes encoding resistance to tetracyclines was most frequently represented by tet(E) and tet(O). Genes encoding virulence ahh1 (heat-labile hemolysin) or integrase intI1 occurred more frequently in the strains isolated from DRW than URW. An analysis of genetic relatedness revealed two main clusters – one with closely related WW and DRW isolates and one with less related isolates from all analyzed samples. The results of this study indicate that regardless of the applied treatment, municipal sewage may be a reservoir of antibiotic-resistant bacteria, antibiotic resistance and virulence genes and that treated water can contaminate other environmental domains.

First Detection of Augmentin and Colistin Resistant <i>Cronobacter Sakazakii</i> from a Pharmaceutical Wastewater in South-Western Nigeria

Cronobacter sakazakii formerly known as Enterobacter sakazakii, is a bacterium with a rare cause but often fatal infection of the bloodstream and central nervous system. Infants with weakened immune systems, particularly premature infants, are most likely to contact a Cronobacter infection, although the bacteria have caused illnesses in all age groups. Most cases of C. sakazakii infection come from powdered infant formula (PIF) contaminated with the bacterium. Although relatively little information is known about the existence of Cronobacter in the environment, more reservoirs are being identified, such as water, soil and plant material. Wastewaters from 6 pharmaceutical industries located in a south-western state in Nigeria were sampled and analyzed. Bacteria were isolated using standard methods and species identification was determined by Gram staining, lactose fermentation, oxidase, catalase and Vitek 2. Antibacterial susceptibility to 25 antimicrobial agents was tested by the disc diffusion method and Vitek 2. Fifty nine Gram-negative bacteria were isolated and identified; one was identified as C. sakazakii. The bacterium was susceptible to all antibiotic mentioned but resistant to augmentin (amoxicillin/clavulanate) and colistin which are high potent drugs for the treatment of very stubborn infections. The public health implication of this fact is that this bacterium could be harbouring resistant genes that can be transferred through water ways such as the pharmaceutical wastewaters to bacteria of the same or different species of clinical importance. Therefore, continuous surveillance of the environmental reservoirs of antibiotic resistant bacteria is necessary to prevent their further spread.

Antimicrobial Susceptibility of Staphylococcus aureus Isolated from Recreational Waters and Beach Sand in Eastern Cape Province of South Africa.

Background: Resistance of Staphylococcus aureus to commonly used antibiotics is linked to their ability to acquire and disseminate antimicrobial-resistant determinants in nature, and the marine environment may serve as a reservoir for antibiotic-resistant bacteria. This study determined the antibiotic sensitivity profile of S. aureus isolated from selected beach water and intertidal beach sand in the Eastern Cape Province of South Africa. Methods: Two hundred and forty-nine beach sand and water samples were obtained from 10 beaches from April 2015 to April 2016. Staphylococcus aureus was isolated from the samples using standard microbiological methods and subjected to susceptibility testing to 15 antibiotics. Methicillin-resistant Staphylococcus aureus (MRSA) was detected by susceptibility to oxacillin and growth on Brilliance MRSA II agar. Antibiotic resistance genes including mecA, femA rpoB, blaZ, ermB, ermA, ermC, vanA, vanB, tetK and tetM were screened. Results: Thirty isolates (12.3%) were positive for S. aureus by PCR with over 50% showing phenotypic resistance to methicillin. Resistance of S. aureus to antibiotics varied considerably with the highest resistance recorded to ampicillin and penicillin (96.7%), rifampicin and clindamycin (80%), oxacillin (73.3%) and erythromycin (70%). S. aureus revealed varying susceptibility to imipenem (96.7%), levofloxacin (86.7%), chloramphenicol (83.3%), cefoxitin (76.7%), ciprofloxacin (66.7%), gentamycin (63.3%), tetracycline and sulfamethoxazole-trimethoprim (56.7%), and vancomycin and doxycycline (50%). All 30 (100%) S. aureus isolates showed multiple antibiotic-resistant patterns (resistant to three or more antibiotics). The mecA, femA, rpoB, blaZ, ermB and tetM genes were detected in 5 (22.7%), 16 (53.3%), 11 (45.8%), 16 (55.2%), 15 (71.4%), and 8 (72.7%) isolates respectively; Conclusions: Results from this study indicate that beach water and sand from the Eastern Cape Province of South Africa may be potential reservoirs of antibiotic-resistant S. aureus which could be transmitted to exposed humans and animals.

The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China).

Antibiotic failure is occurring worldwide. In a routine surveillance study on antibiotic resistance genes (ARGs) in natural water bodies, we noted the detection of colistin-resistance gene mcr-1, previously identified in Escherichia coli and Klebsiella pneumoniae isolates from human beings and animals in several countries. The mcr-1 gene might be present in water environments, because aquatic ecosystems are recognized as reservoirs for antibiotic resistant bacteria (ARB) and ARGs. In this study, a qPCR assay was developed to monitor and quantify the mcr-1 gene in the Haihe River, China. The results showed that all 18 samples collected from different locations over 6 months along the Haihe River were positive for the mcr-1 gene, and the highest level of mcr-1 reached 3.81 × 105 gene copies (GC) per liter of water. This is the first study to quantify mcr-1 in a natural water system by qPCR. Our findings highlight the potential for this antibiotic resistance determinant to spread extensively, suggesting a significant health and ecological impact.

The effects of low-level ionizing radiation and copper exposure on the incidence of antibiotic resistance in lentic biofilm bacteria

Environmental reservoirs of antibiotic resistant bacteria are poorly understood. Understanding how the environment selects for resistance traits in the absence of antibiotics is critical in developing strategies to mitigate this growing menace. Indirect or co-selection of resistance by environmental pollution has been shown to increase antibiotic resistance. However no attention has been given to the effects of low-level ionizing radiation or the interactions between radiation and heavy metals on the maintenance or selection for antibiotic resistance (AR) traits. Here we explore the effect of radiation and copper on antibiotic resistance. Bacteria were collected from biofilms in two ponds – one impacted by low-level radiocesium and the other an abandoned farm pond. Through laboratory controlled experiments we examined the effects of increasing concentrations of copper on the incidence of antibiotic resistance. Differences were detected in the resistance profiles of the controls from each pond. Low levels (0.01 mM) of copper sulfate increased resistance but 0.5 mM concentrations of copper sulfate depressed the AR response in both ponds. A similar pattern was observed for levels of multiple antibiotic resistance per isolate. The first principal component response of isolate exposure to multiple antibiotics showed significant differences among the six isolate treatment combinations. These differences were clearly visualized through a discriminant function analysis, which showed distinct antibiotic resistance response patterns based on the six treatment groups.