Research Institute Research Articles

The Experience Using of Freeze-Dried Mulberry Leafs’ as Composition of Artificial Diet for Feeding Silkworm Caterpillars

Aims: Due to changes in the global climate and the recent increase in spring frosts, which lead to the death of silkworm caterpillars of the first ages due to lack of food, there is an urgent need to find effective and cheap means to replace natural food - mulberry leaf. One of the promising methods is the preservation of mulberry leaf. One of the new and progressive methods of preservation is the method of freeze-dried of mulberry leaf, for the basis of artificial diet. Place and Duration of Study: The experiments were conducted in the laboratory of the Scientific Research Institute of Sericulture in 2024y. Methodology: After hatching, the caterpillars were fed artificial food based on freeze-dried mulberry leaf, the food was given once a day, the room temperature was 25-26 ° C, humidity 65-70%. When the caterpillars reached the third age, some of the caterpillars of each breed and hybrid were selected and continued to be fed with artificial diet. The remaining caterpillars were transferred to natural leaf feeding according to a generally accepted method. Results: The drying time of the mulberry leaf in the freeze-dried mode was 14-18 hours, depending on the uniformity of the leaf layer in the chamber. A recipe for artificial diet was created, in which the content of mulberry leaf powder was 72%. All breeds and hybrids equally well received the developed artificial diet. In breeds and hybrids, the viability of caterpillars was 82-100%. And when switching from artificial diet to a natural leaf, the viability of caterpillars was 63-93%. Conclusion: All breeds and hybrids equally well accepted the developed artificial food based on freeze-dried mulberry leaf. According to the totality of the results shown, the studied breeds and hybrids are promising for feeding them on artificial feed and breeding appropriate lines and breeds from them.

Two cnidian plastic vessels of the 2nd century ad from the collection of the state historical museum: technological research

The article considers two textbook-famous Cnidian plastic vessels from the collection of the State Historical Museum in Moscow. One of them, shaped as a ram, comes from the excavations of N.I. Veselovsky in the Kuban region, the second one – a lagynos with erotic scenes on its body – was found by I.I. Gushchina at the Late Scythian burial ground Belbek IV in the Southwestern Crimea. As part of a project to do research on monuments of the classical period from the State Historical Museum collection, an X-ray tomographic study of these vessels was conducted at the National Research Centre “Kurchatov Institute”, which made it possible to clarify the technology of their manufacture.

Spring growth of lucerne (Medicago sativa L.) after different times of winter defoliation

The aim of this work was to determine if autumn/winter defoliation affected the time of lucerne stem extension in spring. Two experiments were established at the Field Research Centre, Lincoln University. Experiment 1 was established on 01/06/2022 using an existing two year old stand of “Force 4” lucerne. There were four canopy defoliation dates (1/06, 03/07, 17/07, 1/08). Experiment 2 repeated the treatments on the same paddock in the following year on eight defoliation dates (1/5, 15/5, 1/6, 15/6, 3/7, 17/7, 1/8 and 15/8). In both experiments, five stems were marked in each plot and stem height was measured weekly. The start of stem extension was unaffected by canopy defoliation date in either 2022 (P = 0.20) or 2023 (P = 0.17). Initiation of stem extension was triggered by a base photoperiod of 11.1 ± 0.2 h. This meant the thermal time accumulated from final defoliation to the start of stem extension differed among treatments. These results suggest the time of autumn/winter defoliation (the winter clean-up graze) will not affect the time of the first spring grazing, provided the developing basal buds are not removed. All stands reached 150 mm height by mid-September and produced 3.0 t/ha of dry matter by the end of the month. This suggests, grazing of the first paddock at Lincoln should commence in the first week of September to create a staggered start to rotational grazing that can maximise lucerne yield and quality for animal liveweight production.

Comprehensive research facility for negative ion source neutral beam injection at CRAFT: design and first operations

Abstract Neutral beam injection (NBI) has been proven as a reliable heating and current drive method for fusion plasma. For the high-energy NBI system (particle energy > 150 keV) of large-scale fusion devices, the negative ion source neutral beam injection (NNBI) system is inevitable, which can obtain an acceptable neutralization efficiency (> 55%). But the NNBI system is very complex and challengeable. To explore and master the key NNBI technology for future fusion reactor in China, an NNBI test facility is under development in the framework of the Comprehensive Research Facility for Fusion Technology (CRAFT). The initial goal of CRAFT NNBI facility is to achieve a 2 MW hydrogen neutral beam at the energy of 200–400 keV for lasting 100 s. In the first operations of the CRAFT NNBI facility, a negative ion source with dual RF drivers was developed and tested. By using the 50 keV accelerator, the long-pulse and high-current extractions of negative hydrogen ions have been achieved and the typical values were 55.4 keV, 7.3 A (~ 123 A/m2), 105 s and 55.0 keV, 14.7 A (~ 248 A/m2), 30 s, respectively. By using the 200 keV accelerator, the megawatt-class negative hydrogen beam has also been achieved (135.9 keV, 8.9 A, 8 s). The whole process of the gas neutralization of negative ion beam, electric removal of residual ions, and beam transport have been demonstrated experimentally.

Communication behavior and space utilization of office workers in a research facility's activity‐based workplace

AbstractThis study explores the pre‐ and post‐renovation differences in communication behaviors of office workers at a construction company's research facility that had carried out renovations to promote open innovation through internal and external interactions. We conducted an analysis by combining both objective survey of conversation time, conversation partners, and stay location information using sensing terminals and subjective evaluations by office workers using a questionnaire survey. As the work style transitioned from fixed seats pre‐renovation to activity‐based working post‐renovation, the attendance rate analysis around each worker's personal workspace throughout the measurement period decreased from 74% to 60%, and the tendency to use various places in the office increased. Additionally, the number of conversation partners increased by 1.9 times on average and inter‐department conversation rate increased from 46% to 53% when activity‐based working was performed post‐renovation. However, while the introduction of activity‐based working significantly increased the level of communication satisfaction among workers in other departments, it tended to decrease the level of communication satisfaction within a department. This study maintains that organizational and operational efforts must sustain and promote intra‐departmental communication when activity‐based working is introduced and confirms that the introduction of activity‐based working was effective in promoting interaction between office workers.

Innovative approaches to risk management in clinical research: Balancing ethical standards, regulatory compliance, and intellectual property concerns

This study explores innovative approaches to risk management in clinical research, focusing on the intricate balance between ethical standards, regulatory compliance, and intellectual property (IP) concerns. As clinical trials become increasingly complex and globalized, managing risks effectively while maintaining participant safety and upholding legal and ethical principles has become paramount. The study adopts a comprehensive review of emerging technologies, such as artificial intelligence (AI), machine learning (ML), blockchain, and big data analytics, to assess their role in transforming risk management strategies. Key findings reveal that AI and predictive analytics significantly enhance real-time monitoring and the early identification of risks, while adaptive trial designs offer flexibility in managing unforeseen challenges during trials. Blockchain technology improves transparency and data integrity, ensuring secure and verifiable data transactions. However, the intersection of IP protections and equitable access to treatments presents ongoing ethical challenges, particularly in low-income regions. The study concludes that while technological innovations provide robust tools for mitigating risks, a balanced approach to regulatory frameworks and IP protections is essential. The study recommends increased collaboration between regulatory bodies, research institutions, and technology providers to harmonize global regulatory standards, ensuring both innovation and accessibility. Furthermore, it advocates for more flexible IP frameworks that address the tension between protecting innovations and ensuring equitable access to life-saving treatments.

Re-engineering Agricultural Innovation in Southeast Asia (RAISE-Asia)

Feeding the growing global population and reducing poverty require innovative approaches in agriculture and food systems to sustain the planet’s health and food requirements. Sustainable development can be achieved by generating new knowledge and translating them into use through innovation process. A customer-oriented technology readiness level (COTRL) tool is presented to re-engineer agricultural innovation by systematically assessing new agricultural technologies with customer input and ensuring that the early design of the scientific research already has the end-products in mind. The COTRL methodology will integrate research, prototyping, and commercialization efforts and facilitate a disciplined and impactful innovation process. It will guide researchers and scientists, research managers and coordinators, funding agency program managers, policymakers, and other personnel involved in bringing scientific discoveries to market for societal impact. The COTRL framework can be used to strengthen the collaboration among academia, research institutions, networks of excellence, and the private sector in the ASEAN to create an effective ecosystem for manpower and structural capability development, knowledge sharing, and business development within and across member states.

Visual analysis of postmortem interval estimation trends and collaborative networks: a 15-year study (2006-2020).

Utilizing a visual analysis of the literature on postmortem interval (PMI) estimation indexed by Web of Science (WOS), this study investigates developmental trends and research hot points across each 5-year period from 2006 to 2020. Additionally, collaborative efforts among authors, countries, and institutions were examined. Research hot points, high-frequency keywords, authors, countries and institutions in relevant papers were analyzed using CiteSpace.5.7.R2 information visualization analysis software over the past 15 years. The literature related to PMI estimation has witnessed consistent growth over time. In the keyword co-occurrence network, several impactful terms stand out, including blowfly, mitochondrial DNA, and emerging concepts like virtual autopsy. Technological advancements, such as RNA stability analysis and virtual autopsy tools, have played a pivotal role in shaping the direction of PMI research. Scientific research institutions dominate the high-frequency affiliations within the institutional cooperative network. Additionally, the country cooperative network exhibits a trend of co-occurrence and multi-clustering. As science and technology continue to advance, traditional PMI estimation methods mature while novel interdisciplinary approaches drive innovation. By identifying emerging trends and research hotspots, this study provides a roadmap for future investigations, guiding researchers toward new opportunities in PMI estimation.

Veeraswamy Seshiah: Father of Gestational Diabetes in India

Professor Dr. V. Seshiah, MD, FRCP, DSc, (Hony), is a distinguished professor at Tamil Nadu's Dr. MGR Medical University and established the First Department of Diabetology in India at Madras Medical College in 1978. He was the patron of the Research Society for the Study of Diabetes in India and the founder patron of the Diabetes in Pregnancy Study Group, India (DIPSI). Additionally, he served as the Vice-Chair of the Executive Board of the International Association of Diabetes and Pregnancy Study Group. Dr. V Balaji and Dr. V Seshiah Diabetes Care Center & Research Institute, which he founded, received the Army Decorations "SAMAR SEVA STAR" and "SAINYA SEVA MEDAL" in 1965 with clasp HIMALAYAS. Dr. Seshiah was also the recipient of the DR. B.C.ROY National Award was given in 1988 for developing diabetology as the Indian College of Physicians provided a specialty in the country and the Master Teacher Award. Moreover, he was honored with the Lifetime Achievement Award by the International Diabetes Federation in 2017, being the first Indian to receive this award. In 2022, the President of India, Mr. Kovind, presented the Padma Shri to Dr. Veeraswamy Seshiah for Medicine.

The dry lab microscopist or prompt microscopist: do we need them?

AbstractIn modern biological microscopy, the explosion of data volume and complexity highlights the urgent need for specialised data management support roles. While traditional microscopy focuses on visual data presentation, the rapid increase in big data acquisition and data mining demands advanced handling and analysis. This gap underscores the need for “dry lab microscopists” or data experts skilled in microscopy data management, software interoperability, and AI-driven solutions. Job markets reflect this demand, pointing to the necessity for dedicated training programs. Integrating these specialists into research institutions is crucial for addressing digital data challenges and maintaining high standards in data integrity and analysis. Their role is essential for advancing research in the data-driven era.

THE NONSINGULAR PARADIGM OF ACADEMIC ENTERPRISE: ENSURING QUALITY THROUGH RATIONALITY, NEGOTIABILITY, COMMUNICABILITY, AND SHAREABILITY

Academic enterprises, encompassing universities, colleges, and research institutions, play a pivotal role in advancing knowledge, fostering intellectual growth, and contributing to societal progress. Unlike monolithic organizations, academic institutions operate within a nonsingular paradigm, characterized by diversity in thought, disciplines, and methodologies. The quality of these institutions is ensured by practicing four key principles: rationality, negotiability, communicability, and shareability. This essay explores these principles and how they collectively sustain and enhance the quality of academic enterprises.

Research hotspots and frontiers in non-specific low back pain: a bibliometric analysis

BackgroundExtensive research has been conducted worldwide on non-specific low back pain (NSLBP), some researchers published a bibliometric analysis of NSLBP in 2020, but there have been no supplements or updates since then. Therefore, this study aimed to analyze the research hotspots and frontiers in NSLBP over the last decade.MethodsPrimary sources on NSLBP were obtained from the Web of Science Core Collection database from 2014 to 2023. CiteSpace V6.2. R7 (64-bit) and VOSviewer 1.6.19 software were used to analyze the number and centrality of journals, countries, institutions, authors, references, and keywords, and the functions of co-occurrence and clustering were applied to draw a visual knowledge map.ResultsIn the past decade, the annual publication volume of studies on NSLBP has shown an overall upward trend year by year, with obvious temporal stages and great development potential. In total, 2,103 articles contained six types of literature, with the highest proportion being original research articles (1,633 articles, 77.65%), published in 200 journals. BMC Musculoskeletal Discourses (90 articles, 4.28%) had the highest number of publications, and the British Medical Journal had the highest impact factor (105.7). Furthermore, the United States of America (329 articles, 15.64%) had the highest publication volume, the University of Sydney (139 articles, 6.61%) was the research institution with the highest production, Maher, Chris G (36 articles, 1.71%) was the author with the most published articles, and Hoy, D (571 articles, 27.15%) was the most frequently cited author. The most cited of articles is “Non-specific low back pain” published in the LANCET, with 1,256 citations.ConclusionThis article summarizes the current research status of NSLBP and predicts future research hotspots and frontiers. In recent years, adolescents have become a high-risk group for NSLBP. Pain neuroscience education, motor control, spinal manipulative therapy, and acupuncture are effective means to treat NSLBP. Biomechanics and trunk muscles as entry points are effective ideas for the treatment of NSLBP pain. Furthermore, anxiety, neck pain, non-specific musculoskeletal pain, fibromyalgia, and musculoskeletal disorders are diseases that are closely related to NSLBP. In the future, attention should be paid to the design of research plans, increasing the research intensity of randomized controlled trials, strengthening follow-up, and the timely updating of guidelines, which will result in higher quality and high-level scientific evidence for research on NSLBP.

Histopathological Profile of Cholecystectomy Specimens at a Tertiary Care Center: A Retrospective Study

Abstract Introduction: Gallbladder is one of the most frequently received specimens in any histopathology laboratory. Its diseases may present with a varied spectrum ranging from congenital anomalies, cholelithiasis, and inflammatory or noninflammatory lesions to noninvasive and invasive neoplasms. Cholelithiasis (gallstones) is the most common lesion and accounts for more than 95% of gallbladder diseases and affects 10%–20% of the adult population. The incidence is 2–4 times higher in females than in males. Aims and Objectives: This study was designed to study the histopathological spectrum of gall bladder diseases in the population, study the most common gall bladder diseases in cholecystectomy specimens, and study the frequency of gall bladder carcinoma. Methodology: This retrospective observational study was done in the Pathology department at a tertiary care hospital and research center, from September 2016 to September 2023. Tissue was fixed in 10% buffered formalin and then processed by the routine paraffin embedding techniques. Sections were cut at 4–5 μ thickness and stained with hematoxylin and eosin stains. Special stains were done if required for confirmation. All the slides were analyzed and reported by a trained histopathologist. Results: Females were affected more as compared to males. The commonest age group affected was 41-50 years. Gallstones were observed in 33.58% of patients. Gallbladder neoplasm was rare and incidental findings. Conclusion: Gallbladder disease demonstrated a diverse spectrum of histopathological changes in the cholecystectomy specimens and showed female preponderance. Gallbladder carcinoma was observed as a rare occurrence and was an incidental finding mainly. Hence, a microscopic (histopathological) examination is required for every cholecystectomy specimen.

Assessment of drug therapy problems in pediatric patients using Cipolle/Morley/strand classification: A hospital based study

Drug plays an important role in treating diseases and improving health. However, inappropriate drug use can lead to the occurrence of drug-Therapy problems (DTPs). According to Cipolle/Morley/Strand classification DTPs are any undesirable event experienced by the patient that involves or is suspected to involve drug therapy and that actually or potentially interferes with a desired patient outcome. The purpose of this study was to assess the drug therapy problems in a pediatric ward of Srinivas institute of medical science and research centre and to identify associated factors for drug therapy problems. A retrospective observational study was carried out to assess drug therapy problems (DTPs) in paediatric patients using Cipolle/Morley/Strand classification from January to June 2024. Ethical approval was obtained, and informed consent was signed by each study participant's parent before the commencement of the study. All paediatric out patients and patients admitted to the paediatric ward during the study period were included in the study. Data were collected through medical record reviews of patients and using semi structured questionnaire. Data was analysed using Microsoft Excel. The participants mean age was 2.32 years. Among 150 patients, 116 (77.33%) of them had at least one drug therapy problem per patient which indicates that the prevalence of the drug therapy problem was substantially high. On the other hand, Adverse drug reaction was the least 4 (3.38%) drug therapy problem. Hence to ensure optimum care in paediatric patients, a targeted approach is essential. Thus, the present study concluded that pharmacists can be helpful in the identification of the drug therapy problems, their causes and the risk factors involved and thus lead to a better health care outcome.

Gereja Katolik dan Pengentasan Pengangguran Pemuda di Era Bonus Demografi

Youth unemployment in Indonesia remains notably high at 9.25%, posing a potential threat to the optimal utilization of the demographic dividend anticipated to peak by 2030. This research seeks to explore how the Catholic Church can contribute to addressing youth unemployment during this critical period. By employing the theoretical framework of Catholic social teaching—focusing on the principles of human dignity, solidarity, and integral justice—the study examines the Church's role, potential, and concrete opportunities in mitigating youth unemployment, and provides recommendations for key programs. A qualitative approach is used, involving a literature review of Catholic social teaching documents and analysis of secondary data. The study's findings suggest that the Catholic Church holds strategic potential through its extensive network of parishes and institutions to assist in resolving the youth unemployment issue. Suggested priority programs include skills training, educational scholarships, job fairs, the establishment of youth empowerment institutions, vocational schools, research centers, and business incubators. Effective cross-sector collaboration is crucial to ensuring that these Church-initiated programs function efficiently and offer long- term benefits to Indonesian youth, helping them seize the opportunities presented by the 2030 demographic bonus. This research adds to the understanding of the potential contributions of religious institutions in addressing contemporary socio-economic challenges and opens avenues for further study on the intersection of religion, public policy, and economic development within the framework of Indonesia‘s demographic dividend.

Guangdong’s New R&D Institutes

In pursuit of technological development, China has created new organizations to promote innovation. This brief examines New Research and Development Institutes (NRDIs), which are designed to foster knowledge transfer to industry. NRDIs were pioneered in Guangdong province in the 1990s, and have gained prominence in China’s national science, technology, and innovation policies since the 13th Five-Year Plan (2016–2020). NRDIs are defined by their market orientation and extremely flexible organizational form. They work by establishing “innovation platforms” with local governments and private knowledge actors to carry out research and development (R&D), commercialize scientific and technological achievements, incubate local technology industries, and cultivate high-end talent. NRDIs have been instrumental to regional development in Guangdong, and especially Shenzhen, where they have succeeded in attracting talent from outside the region. NRDIs have important policy implications for international competition for talent. Understanding NRDIs is crucial for other countries that want to improve their own inter-regional innovation resources and respond to the challenge of China’s drive to attract global talent and knowledge resources.

Breakthrough Rectal Neisseria gonorrhoeae Infections After Meningococcal B Vaccination: Microbiological and Clinical Features.

4CMenB appears to be effective in reducing Neisseria gonorrhoeae (Ng) infections. Aims are to assess factors associated with breakthrough rectal Ng after 4CMenB and evaluate clinical and microbiological characteristics of breakthrough infections compared with before vaccination. This was a retrospective study of gay, bisexual, and other men who have sex with men (GBMSM) vaccinated with 4CMenB (2 doses) between 2017 and 2023 at the San Raffaele Scientific Institute for Research, Hospitalization and Healthcare (IRCCS San Raffaele Scientific Institute), Milan, Italy, and tested for rectal Ng. Rectal Ng infection is considered breakthrough if it occurs >1 month after the second 4CMenB dose and with positive nucleic acid amplification test (NAAT) result. Follow-up was from July 2017 (first 4CMenB vaccination) to November 2023 (data freeze). Rectal Ng was screened with both NAAT and gonococcal-specific cultures. Characteristics of individuals with or without breakthrough Ng and of Ng infections before or after 4CMenB were compared using Mann-Whitney and χ2/Fisher tests. Overall, 473 GBMSM vaccinated with 4CMenB were included, with a median age (interquartile range) of 43 (37-51) years; 451 of 473 were living with human immunodeficiency virus. The percentage of NAAT-positive rectal Ng swab samples was 76 of 957 (7.7%) after 4CMenB and 51 of 456 (11.1%) before. Breakthrough rectal Ng after baseline were 76 in 57 of 473 people. People with rectal Ng after 4CMenB were younger, more likely to have a previous sexually transmitted infection, and had more sexual partners than those without (all P < .001). Breakthrough rectal Ng infections were less frequently symptomatic (34.2% vs 66.7%; P = .001) and more likely with negative gonococcal-specific culture (55.3% vs 19.6%; P < .001) compared with before vaccination. Breakthrough rectal Ng infections after 4CMenB were 76 in 57/473 people, preferentially identified in GBMSM with higher-risk sexual behaviors, were less often symptomatic, and more often with negative gonococcal-specific cultures, suggesting lower infection virulence.

Knowledge mapping and research trends of brain-computer interface technology in rehabilitation: a bibliometric analysis

BackgroundAlthough the application of brain-computer interface (BCI) technology in rehabilitation has been extensively studied, a systematic and comprehensive bibliometric analysis of this area remains lacking. Thus, this study aims to analyze the research progress of BCI technology in rehabilitation through bibliometric methods.MethodsThe study retrieved relevant publications on BCI technology in rehabilitation from the Web of Science Core Collection (WoSCC) between January 1, 2004, and June 30, 2024. The search was conducted using thematic queries, and the document types included “original articles” and “review articles.” Bibliometric analysis and knowledge mapping were performed using the Bibliometrix package in R software and CiteSpace software.ResultsDuring the study period, a total of 1,431 publications on BCI technology in rehabilitation were published by 4,932 authors from 1,281 institutions across 79 countries in 386 academic journals. The volume of research literature in this field has shown a steady upward trend. The United States of America (USA) and China are the primary contributors, with Eberhard Karls University of Tübingen being the most active research institution. The journal Frontiers in Neuroscience published the most articles, while the Journal of Neural Engineering was the most cited. Niels Birbaumer not only authored the most articles but also received the highest number of citations. The main research areas include neurology, sports medicine, and ophthalmology. The diverse applications of BCI technology in stroke and spinal cord injury rehabilitation, as well as the evaluation of BCI performance, are current research hotspots. Moreover, deep learning has demonstrated significant potential in BCI technology rehabilitation applications.ConclusionThis bibliometric study provides an overview of the research landscape and developmental trends of BCI technology in rehabilitation, offering valuable reference points for researchers in formulating future research strategies.

Beam Dynamics in the Heavy Ion Injector LU2 for the Synchrotron Research Complex (SRC)

The project of the complex for studying ionizing radiation exposure from outer space based on the synchrotron accelerator of protons and various types of ions up to 209Bi is being developed at the Russian Federal Nuclear Center - All-Russian Research Institute ofExperimental Physics (FSUE RFNC - VNIIEF). The accelerator includes two injection complexes (one of which is the source of protons and light ions, the second - heavy ions), the booster accelerator and the main synchrotron. The pulsed type heavy ions linac is being developed at the NRC “Kurchatov Institute” - KCTEF (Kurchatov Complex for Theoretical and Experimental Physics). The ions with mass-to-charge ratio 4÷8 with current of 10 mA will be accelerated up to 4 MeV/u. The linac is proposed, including the RFQ and two DTL sections operating at multiple frequencies. Each DTL section is modular and consists of individually phased H-type resonators (IH-DTLs). Quadrupole lenses located between the resonators for the beam focusing. This DTL structure ensures the accelerator compactness and allows section-by-section configuration and sequential commissioning. 6D beam matching between all sections of the linac is carried out. The results ofbeam dynamics simulation in linear accelerator are presented.

A Prospective Observational Study on Analyzing Lung Cancer Gene Mutation Variant Allele Frequency (VAF) and Its Correlation with Treatment Efficacy

A few studies have reported on the variability of the variant allele frequency (VAF) of gene mutations and the relationship between VAF and the therapeutic effect of molecular-targeted drugs. This joint study was conducted from May 2020 to January 2022 between St. Marianna University School of Medicine and the DNA Chip Research Institute. Cytology samples were used to verify the usefulness of a lung cancer compact panel test, which is a high-sensitivity next-generation sequencing (NGS) gene panel test. We analyzed the distribution of VAF and the duration of the initial molecular-targeted drug administration in patients with advanced-stage epidermal growth factor receptor (EGFR) mutations. Of the 196 patients diagnosed with non-small cell carcinoma (NSCLC), gene mutations were detected in 114 (58.2%) cases and in 68.7% of patients with adenocarcinomas (an increased detection rate). A VAF of 30% or more for DNA mutations was confirmed in 35 patients (33.7%), 10% to &lt;30% in 38 (36.5%), and &lt;10% in 31 (29.8%), including 18 patients (17.3%) with &lt;6%, which is considered the lower limit of the LOD for other companion diagnostic kits. EGFR mutations were detected in 59 patients (30%), of whom 35 were in an advanced stage and received molecular-targeted drugs as their initial treatment. Groups A and B comprised patients with a VAF of ≥10% and &lt;10%, respectively, with 27 patients (median VAF 30.6%, range 11.4–77%) in group A and 8 (median VAF 5.4%, range 0.1–8.5%) in group B. The duration of the administration of molecular-targeted drugs was 17 months (range 3–42 months) in group A and 14 months (range 1–45 months) in group B, with no statistically significant difference between the two groups (p-value = 0.7). Twenty-seven percent of patients had a VAF &lt; 10%. Even in patients with a low VAF for gene mutations, sufficient therapeutic effects were observed with molecular-targeted drugs, suggesting the importance of detecting mutations using a highly sensitive method at initial diagnosis. Further prospective validation studies of a larger number of cases are warranted.