The survey conducted under the Ancient Meadows and Pastures project represents a new approach in nature conservation in Sweden, where natural and cultural heritage values were inventoried and analysed in an integrated holistic approach between 1985 and 1997. The model for the survey, developed jointly by researchers and planners, is composed of four steps: methodological development of data collection; national survey and regional ranking; national analysis; and applications. This paper describes the conceptual model, the methodological development and national analysis. The method development is composed of strategic selection of criteria for natural and cultural values, development of classification systems, development of techniques for aerial photo interpretation, methods for fieldwork and description, evaluation and ranking. The data collection is based on interpretation in stereo pairs of colour infrared (CIR) aerial photographs with selection of potentially valuable meadows and pastures which are inventoried and checked in the field for cultural-historical nature types, cultural elements, vegetation types and selected indicator species of vascular plants. They are also described from a holistic landscape ecological perspective, integrating the cultural and biological components within the entire landscape and, finally, ranked for nature conservation into four classes. The evaluation emphasises botanical values together with long-term continuity in land-use and management, underpinned with cultural components. The national analysis showed that nearly 200,000 ha of ancient meadows and pastures are well managed and of high value. They are distributed among more than 17,000 objects and 22 cultural-historical nature types. Most were pastures (98%). The result is not only increased knowledge of the distribution of ancient meadows and pastures and a high number of well-managed pasture landscapes throughout Sweden, but also a change in attitude among farmers, towards more positive attitudes to nature conservation and biodiversity and understanding of the cultural landscape. This project has had an important influence, because it is used in regional nature conservation work, has formed the principal basis of several thousands of management agreements with farmers, is used in policy work in the national nature protection plan and in the biodiversity action plan. The database also provides a good foundation for research in cultural landscapes, biodiversity and landscape ecology.