Scientific Community Research Articles

Targeting ultra-processed foods for prevention of type 2 diabetes: state of the evidence and future directions.

The incidence of type 2 diabetes has risen globally, in parallel with the obesity epidemic and environments promoting a sedentary lifestyle and low-quality diet. There has been scrutiny of ultra-processed foods (UPFs) as a driver of type 2 diabetes, underscored by their increasing availability and intake worldwide, across countries of all incomes. This narrative review addresses the accumulated evidence from investigations of the trends in UPF consumption and the relationship with type 2 diabetes incidence. Hypotheses for why UPFs may be causally implicated in the initiation and progression of weight gain and suboptimal blood glucose levels are varied. There is also uncertainty and debate about whether detrimental effects of UPFs could be owing to additives and other features of industrial processing, independent of established dietary risk factors, namely added sugar, sodium, saturated fat and low fibre content. However, these current research gaps are addressable with rigorous research and coordinated efforts across nutrition-science domains; for example, the strengths of longitudinal cohort studies can be leveraged to refine the characterisation of key UPF subcategories within the enormous and diverse category of UPFs and ultra-processed beverages, and to identify high-risk patterns of intake that are related to the development of chronic-disease outcomes. The notable advantages of dietary intervention studies are the critical gains in the reliability of dietary assessments, and isolating the effects of individual UPF additives and features through carefully formulated diets. Research improving our understanding of the modifiable food environment, the diet's causal drivers of weight gain and suboptimal cardiometabolic health, and the interactions among them, can be used to meaningfully shift the food supply for large-scale improvements in health. Thus, although the global market share of UPFs seems to outpace the research on its detrimental health effects, leaving the scientific community with the responsibility of generating proof, there may still be promising opportunities to reduce the burden of type 2 diabetes.

Optimizing survey conditions for Burmese python detection and removal using community science data

Burmese pythons (Python bivittatus) have demonstrated prolific spread and low detectability within their invasive range in Florida, USA. Consequently, programs exist which incentivize contractors to remove pythons. While surveying, contractors collect data on search effort and python captures. We examined data from South Florida Water Management District’s Python Elimination Program to determine the effect of operational and environmental covariates on two measures of survey outcome: success (i.e., probability of removing at least one python) and efficiency (i.e., the number of pythons removed per survey hour). Additionally, we assessed the spatial distribution of contractor search effort and removals. Warm temperatures (> 25 °C) improve survey outcomes, especially when surveys occur late at night and during the wet season (May–Oct). The most efficient interval for conducting surveys occurs from 20:00 to 02:00. The spatial distribution of python removals is concentrated in four regions and coincides with contractor search effort. Our results provide insights into optimizing removal efforts for invasive Burmese pythons in Florida, which may allow for increases in removal efficiency. Moreover, this study demonstrates that community science data can be used to synthesize recommendations for invasive species removal efforts.

The Courage of Public Reason: Sergei Alexandrovich Tokarev as a Historiographer

S. A. Tokarev founded the Russian historiographical tradition, laying the conceptual framework for studying the history of Russian ethnography (its pre-Soviet period), and the origins and history of foreign ethnology. Here we regard historiography in its professional sense and not just as a functional appendage of scientific research. In the author’s interpretation, historiography is not only (and not so much) a narrative about the past, but rather an internal dialogue of science and its self-reflection, a refined form of research into the past and modernity. S. A. Tokarev’s appeal to this genre dates back to the turn of the 1940s–1950s, when a unique constellation of conditions for a successful historiographical search developed. These conditions were rooted both in the internal scientific dynamics (ethnography had acquired a stable status of an independent discipline and a high degree of maturity of professional discourse) and in external circumstances (here the ideological vector coincided with the public sentiment — the struggle against cosmopolitanism on the one hand and the legitimate pride of the victorious people on the other). In this context it was crucial to create a respectable historiographical tradition, which would highlight the significant (and in the Soviet era - the leading) role of Russian science in the global context. The author’s interest is not in the analysis of S. A. Tokarev’s historiographical concept, but rather in the circumstances and conflicts (interdisciplinary, political and ideological) surrounding the preparation and publication of vast historiographical works. The article touches upon the usually neglected topic about scientific preferences and the specifics of a scientist’s research strategy as a projection of his psychotype. Why was it Tokarev who make the historiographical breakthrough? How was the “research laboratory” of a Soviet scientist organized, constrained by the rigid framework of planning, by external (political and ideological) and internal (scientific community) censorship, and under conditions of chronic academic and teaching overload? These issues are of considerable scientific interest.

Studies on the treatment of anaerobically digested sludge by white-rot fungi: evaluation of the effect of Phanerochaete chrysosporium and Trametes versicolor

BackgroundThe composition of anaerobically digested sludge is inherently complex, enriched with structurally complex organic compounds and nitrogenous constituents, which are refractory to biodegradation. These characteristics limit the subsequent rational utilization of resources from anaerobically digested sludge. White-rot fungi (WRF) have garnered significant research interest due to their exceptional capacity to degrade complex and recalcitrant organic pollutants. However, the exploration of WRF in the context of sludge treatment remains an under-investigated area within the scientific community. The present investigation explores the application of WRF in the treatment of anaerobically digested sludge, offering a novel approach for the valorization of sludge resources.ResultsIn this study, WRF enzymes, manganese peroxidase (MnP) and lignin peroxidase (LiP), exhibited sustained high activities of approximately 102 U/L and 26 U/L, respectively, within the anaerobically digested sludge under a controlled pH of 5.5 within the growth system. These conditions were found to significantly enhance the treatment efficacy of the anaerobic sludge. The removal of soluble chemical oxygen demand (COD) and Total COD by Trametes versicolor powder was better than that of Phanerochaete chrysosporium powder. The treatment of sludge samples with WRF, specifically Phanerochaete chrysosporium powder, resulted in a significant reduction of ultraviolet radiation (UV254). Fourier-transform infrared spectroscopy (FTIR) analysis revealed that the application of Trametes versicolor powder exerted a notably pronounced impact on the functional groups present in sludge samples. Specifically, there was a significant decrease in the peak intensities corresponding to the C-O bonds, indicative of saccharide degradation, alongside an observable increase in the intensities of amide peaks, which is suggestive of protein synthesis enhancement. Microbial community analysis demonstrated that Phanerochaete chrysosporium was the predominant fungal species, exerting a significant regulatory role within the sludge ecosystem.ConclusionIn conclusion, this research furnishes a robust scientific foundation for the utilization of WRF in the treatment of anaerobic digestion sludge. It elucidates the fungi’s capacity to ameliorate the physicochemical attributes and microbial community composition within the sludge. Furthermore, the study offers a certain reference for the subsequent use of WRF in the treatment of other types of sludge.

Nucleolin a Central Player in Host Virus Interactions and its Role in Viral Progeny Production.

Nucleolin (NCL) is a prevalent and widely distributed nucleolar protein in cells. While primarily located in the nucleolus, NCL is also found within the nucleoplasm, cytoplasm, and even on the cell surface. NCL's unique nature arises from its multifaceted roles and extensive interactions with various proteins. The structural stability of NCL is reliant on protease inhibitors, particularly in proliferating cells, indicating its essential role in cellular maintenance. This review is centered on elucidating the structure of NCL, its significance in host-viral interactions, and its various contributions to viral progeny production. This work is to enhance the scientific community's understanding of NCL functionality and its implications for viral infection processes. NCL is highlighted as a crucial host protein that viruses frequently target, exploiting it to support their own life cycles and establish infections. Understanding these interactions is key to identifying NCL's role in viral pathogenesis and its potential as a therapeutic target. Our current knowledge, alongside extensive scientific literature, underscores the critical role of host proteins like NCL in both viral infections and other diseases. As a target for viral exploitation, NCL supports viral replication and survival, making it a promising candidate for therapeutic intervention. By delving deeper into the intricacies of NCL-viral protein interactions, researchers may uncover effective antiviral mechanisms. This review aspires to inspire further research into NCL's role in viral infections and promote advancements in antiviral therapeutic development.

The occurrence of introduced rosy red minnows (<i>Pimephales promelas</i>) in Alberta, Canada

Rosy red minnows are a golden morph of Fathead Minnow (Pimephales promelas) commonly sold in pet shops and used, in some jurisdictions, as baitfish. They have formed several naturalized populations in British Columbia, where they are considered a priority conservation concern. The Government of Alberta had noted two occurrences of rosy red minnows in 2016 and 2017, but their identification could not be independently confirmed without photographic or physical vouchers. Based on our own collections, Royal Alberta Museum specimens, community science initiatives, and enquiries to other field researchers, we report that rosy red minnows have been present in Alberta earlier than previously thought, are likely breeding in Alberta, and have a widespread distribution from Fort McMurray to Lethbridge. Given that Alberta has native populations of wild-type Fathead Minnow, the impacts of rosy red minnows on native populations requires immediate attention.

Benefits Of Omega-3 In Peruvian Anchoveta: A Review Of Its Impact On Health

Objectives: The objective of the research was to analyze the current state of knowledge about Omega-3 in the Peruvian anchovy to understand its positive effects on health and promote its adequate consumption. Theoretical framework: The Omega-3 fatty acid present in the Peruvian anchovy is widely known by the scientific community, but its information has not been disseminated enough to know its positive effects in the treatment of inflammatory and autoimmune diseases. Method: The positive effects of Omega-3 fatty acid on health were explored from various perspectives, highlighting its anti-inflammatory action and potential in the prevention and treatment of chronic diseases. Results and discussion: The anchovy, abundant in the Peruvian sea and economic for its availability of catch by the fishing fleet, offers an accessible alternative without compromising the nutritional content, especially relevant in regions with low economy where other sources of Omega-3 are unreachable. It is concluded that anchovy is an accessible source of Omega-3 with considerable nutritional and economic value. Consumption improves public health by preventing chronic and autoimmune diseases. Implications of research: Promote public health policies that include anchovy to maximize its benefits to society, especially in vulnerable populations by ensuring food security and access to low-cost animal protein. Originality/Value: It is essential to promote public health policies that include anchovy in accordance with the ecosystem approach applied to fisheries, food security and sustainable development goals. a rethinking of dietary recommendations is suggested, especially in vulnerable populations in Peru.

Investigating the soft and hard limits in transverse momentum spectra in pp collisions

Abstract The transverse momentum spectra and their multiplicity dependence serve as key tools for extracting parameters to be compared with theoretical models. Over the past decade, the scientific community has extensively studied the possibility of a system analogous to quark-gluon plasma, predicted in heavy nuclei collisions, also existing in collisions involving light nuclei and protons. We have reanalysed the data published by the ALICE Collaboration at the LHC. We present the dependence of the mean transverse momenta obtained in the soft and soft+hard (mixed) parts. Finally, we also discuss possible refinements of the analyses concerning the use of statistical parameters of higher order, aimed at a more detailed way of comparing the models with data.

Artemisia herba-alba Essential Oil: Chemical Composition, Phytotoxic Activity and Environmental Safety

Weeds cause a decrease in the quantity and quality of agricultural production and economic damage to producers. The prolonged use of synthetic pesticides causes problems of environmental pollution, the possible alteration of agricultural products and problems for human health. For this reason, the scientific community’s search for products of natural origin, which are biodegradable, safe for human health and can act as valid alternatives to traditional herbicides, is growing. Essential oils can have useful implications in agriculture by acting as effective alternatives to chemical herbicides. In this work, the chemical composition of an EO from Artemisia herba-alba and its herbicidal properties were studied on two weeds (Lolium multiflorum and Trifolium pratense) and two crops (Brassica napus and Hordeum vulgare) and its environmental safety was also assessed using three model organisms: Chaoborus sp., Tubifex tubifex and Eisenia foetida. The principal component of the EO was camphor (26.02%), with α- and β-thujone (9.60 and 8.38%, respectively), 1,8-cineole (8.02%), piperitenone (5.29%) and camphene (4.95%) as the main components. The EO demonstrated variable phytotoxic effects with a dose-dependent manner, inhibiting both the germination and the radical elongation of the tested seeds, and was also found to be environmentally safe for the selected organisms. The results lay the foundation for considering this EO as a potential weed control agent.

Assessment of quality of presentation of clinical trial results in Russia in accordance with CONSORT standards

The gold standard for presenting evidence of the effectiveness and safety of medical procedures is randomized controlled trials (RCTs). For the research results to be available to other specialists and have potential weight in the scientific community, they must be described and published in accordance with generally accepted international standards, CONSORT, or Consolidated standards of reporting trials.Objective: to analyze the quality of presenting the results of clinical trials in the Russian Federation in accordance with CONSORT standards.Materials and methods. The following Russian journals were selected for the study: "Scientific and Practical Rheumatology", "Good Clinical Practice", "Social and Clinical Psychiatry", "S. S. Korsakov Journal of Neurology and Psychiatry". The selection of target journals was based on two main indicators of the journal rating in the Russian Science Citation Index; according to the SCIENCE INDEX rating and the rating of psychiatric journals by the impact factor RSCI 2016. Journals in the fields of biomedicine (Scientific and Practical Rheumatology), clinical pharmacology (Good Clinical Practice), and journals in the fields of psychology and psychiatry (Social and Clinical Psychiatry, S. S. Korsakov Journal of Neurology and Psychiatry) were selected. Each article was assessed according to the CONSORT checklist items, and the presence or absence of data on the presented items was noted. When studying the articles, the presence or absence of a flowchart in the article was also analyzed, which significantly improves the quality of the written article and compliance with international standards for the design of articles for medical journals.Results. The articles analyzed with the results of RCTs in journals met most of the necessary criteria. The authors paid the least attention to such details of the methodology when publishing a description of the sample calculation (2 out of 5 publications), the type of randomization (2 out of 5 publications), the mechanism of blinding participants (3 out of 5 publications), and implementation and masking (1 out of 5 publications). The inaccessibility of this information may have distorted the reader’s perception and trust in the results.Conclusion. The results show that the reviewed Russian medical journals declare requirements and recommendations for the publication of articles in accordance with the CONSORT standards. To increase readers' confidence in the practical and scientific significance of RCTs, it is necessary to consider the CONSORT requirements and strive to follow them.

Evaluating the antibacterial efficacy of bee venom against multidrug-resistant pathogenic bacteria: Escherichia coli, Salmonella typhimurium, and Enterococcus faecalis

Bee venom (BV) represents a promising natural alternative to conventional antibiotics, particularly significant given its broad-spectrum antimicrobial activity and potential to address the growing challenge of antimicrobial resistance. The prevalence of antimicrobial-resistant microorganisms (AMR) is a global burden that affects human health and the economies of different countries. As a result, several scientific communities around the world are searching for safe alternatives to antibiotics. In this context, the present study represents a comprehensive investigation to evaluate the antibacterial effect of bee venom (BV) against Escherichia coli ATCC8739, Salmonella typhimurium ATCC14028, and Enterococcus faecalis ATCC25923. One mg of BV was extracted using 1 mL of DMSO to obtain a 1000 µg/mL solution. The chemical profile of the BV extract was determined using GC-MS, which revealed the presence of bioactive molecules with antimicrobial properties, such as astaxanthin, hycanthone, and fucoxanthin. The BV extract was tested against bacterial strains using different concentrations to obtain the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results obtained revealed a high antibacterial activity of BV against the three strains with the highest MIC/MBC values of 12.5/25 µgml− 1 against S. typhimurium. The antibacterial activity of the BV extract was compared to five conventional antibiotics using the disc diffusion method. The results showed a high antibacterial activity of the BV extract compared to different antibiotics with the largest inhibition zone obtained against E. faecalis at a value of 15 ± 0.22 mm compared to 9 ± 0.13 for azithromycin. The mode of action of BV, examined using scanning electron microscopy, proved a high effect of BV on the permeability of the bacterial plasma membrane. This study demonstrates bee venom’s promising potential as a natural and eco-friendly antimicrobial agent, with activity against multiple bacterial strains, suggesting it may serve as an alternative to conventional antibiotics. The findings highlight the potential applications of BV in medical, agricultural, and veterinary fields, offering a sustainable solution to combat antimicrobial resistance. However, further studies are needed to fully assess its broad-spectrum antibacterial potential.Clinical trial number Not applicable.

Community-based monitoring: shoreline change in Southwest Alaska

Arctic amplification of climate change has resulted in increased coastal hazards impacts to remote rural coastal communities in Alaska where conducting research can be difficult, requiring alternate methods for measuring change. The pilot program, Stakes for Stakeholders, was initially planned to be funded from 2016–2018. Upon project completion the work has shifted to individual community’s partnering with several agencies to continue the work. Stakes for Stakeholders was initially planned to be funded for three years and upon project completion the work has shifted to individual community’s partnering with several agencies and entities to continue the work. This research showcases a successful long-term community-based erosion monitoring program in two rural communities in Southwest Alaska. The resulting outputs from the workflow we developed were (1) locally prioritized data products, such as a hazard assessment report for Chignik Bay and (2) evaluation rubrics used to assess the suitability of future sites and the efficacy of the program. Our model of two-way communication, responsiveness to individual community needs, and attention to efficiency and effectiveness of the program workflow, can serve as a model for universities, for-profit, non-profit, Tribal, city, state, and federal research agencies and communities partnering to respond to global climate change.

Drosophila CG11700 may not affect male fecundity-lifespan tradeoff as previously reported.

Our recent investigations on the function of Drosophila CG11700 and CG32744 (Ubi-p5E) genes using CRISPR/Cas9 deletion technology could not repeat or confirm the results on CG11700 shown in our previous study which was based on P-element excision assay (Zhan et al. 2012). Here by CRISPR/Cas9 editing, we generated mutants of CG32744 with the whole gene body fully deleted from the genome, and truncated mutants of CG11700 with N-terminal 103 aa deleted out of its total 301 aa peptide sequence. We carefully conducted the male fecundity assay and found that offsprings of the CG11700 mutant were not significantly more than the wild type, inconsistent with our previous report (Zhan et al. 2012). Meanwhile, we repeated the lifespan assay and did not find that the lifespan of the CG11700 mutant is significantly shorter than the wild type as reported in Zhan et al. either (2012). The new results suggest that the CG11700 gene may not affect male fecundity-lifespan tradeoff as previously reported (Zhan et al. 2012). The new results are thus worthy of reporting to avoid possible misleading by the previous results to the scientific community.

Pseudopterosin Biosynthesis: Unravelling a Decades Old Problem in Animal Specialized Metabolism.

Soft corals are prolific producers of terpenoids, such as pseudopterosins. The exact biosynthetic pathway of these anti-inflammatory diterpene glycosides has eluded the scientific community for decades. Using a forward genetic approach, we have identified, cloned, and expressed the key genes involved in pseudopterosin biosynthesis. We characterized a unique class of multifunctional cytochrome P450 enzymes that catalyze a cascade reaction that produces a nearly mature natural product using a single enzyme. This clarifies the previously proposed biosynthetic pathways to pseudopterosin A and its relatives. The mechanism of the oxidative cascade was probed using in vivo feeding studies in Saccharomyces cerevisiae expressing heterologous coral genes. The cascade produces the pseudopterosin aglycone 7,8-dihydroxyerogorgiaene via elisabethatrienol and its epimer, starting from elisabethatriene. This discovery demonstrates the potential to produce this valuable class of natural products using fermentation.

Strategic tips to successfully undertake research: a comprehensive roadmap for medical trainees.

Engaging in research during medical training is crucial for fostering critical thinking, enhancing clinical skills, and deepening understanding of medical science. Despite its importance, the shortage of physician-scientists lingers with many trainees and junior doctors encountering challenges navigating the research process. Drawing on current literature, this article provides a comprehensive roadmap, categorising 12 actionable strategies into five themes, to help medical trainees overcome common obstacles and optimise their research experience. The strategies include early planning, research conduct and integrity, productivity and time management, collaboration and dissemination, and personal growth and development. By implementing these evidence-based recommendations, derived from current literature and expert insights, medical trainees can refine their research skills, produce high-quality outputs, and contribute meaningfully to the scientific community, ultimately enriching their medical training and future careers.

Sustainable Synthesis of Medicinally Important Heterocycles.

Sustainable chemistry and nature-friendly protocols are not only luxury but has become essential requirement for the modern society as it progresses towards a more responsible future. To match the current needs of the community, industries and in particular chemical industry is looking for novel and cheap strategies that have less adverse effects on the environment. Heterocyclic compounds are one particular motif, which is prevalent in nature. It is found in a wide range of synthetic and natural compounds, both established and in development as potent therapeutic candidates. According to the US retail market in 2014-2015, heterocyclic moieties constitute the basic skeletons for 80% of marketed pharmaceuticals. However, majority of the synthetic methodologies still uses classical approaches with toxic solvents, stoichiometric reagents, reactions with less atom economy etc. Thus, there is an urgent need for green, sustainable alternatives of the classical reactions. In recent years, an array of diverse approaches and technologies have been discovered by the scientific community to address the issue of eco-friendly manufacture of various pharmaceutically and medicinally important heterocyclic molecules. In this context, the current review will summarize various reported green pathways to the heterocyclic architecture, particularly O, N, and S-heterocyclic compounds. The methods highlighted in this article includes reaction in environment friendly nonconventional media, solvent-free approaches, heterogeneous catalysis, organocatalysis, electrochemical reactions, microwave-mediated reactions, ultrasound-based reactions, enzymatic reactions, biocatalysis, and others.

Guidelines for Gene and Genome Assembly Nomenclature.

The rapid increase in the number of reference-quality genome assemblies presents significant new opportunities for genomic research. However, the absence of standardized naming conventions for genome assemblies and annotations across datasets creates substantial challenges. Inconsistent naming hinders the identification of correct assemblies, complicates the integration of bioinformatics pipelines, and makes it difficult to link assemblies across multiple resources. To address this, we developed a specification for standardizing the naming of reference genome assemblies, to improve consistency across datasets and facilitate interoperability. This specification was created with FAIR (Findable, Accessible, Interoperable, and Reusable) practices in mind, ensuring that reference assemblies are easier to locate, access, and reuse across research communities. Additionally, it has been designed to comply with primary genomic data repositories, including members of the International Nucleotide Sequence Database Collaboration (INSDC) consortium, ensuring compatibility with widely used databases. While initially tailored to the agricultural genomics community, the specification is adaptable for use across different taxa. Widespread adoption of this standardized nomenclature would streamline assembly management, better enable cross-species analyses, and improve the reproducibility of research. It would also enhance natural language processing applications that depend on consistent reference assembly names in genomic literature, promoting greater integration and automated analysis of genomic data. This is a good time to consider more consistent genomic data nomenclature as many research communities and data resources are now finding themselves juggling multiple datasets from multiple data providers.

Redacción de artículos de revisión para fortalecimiento de habilidades investigativas en universitarios: una mirada desde las nuevas dimensiones de IA

The ability of university students to write scientific texts has become one of the priority areas in higher education. Nevertheless, it is important to complement these skills with new advancements in information technology, especially with the emergence of artificial intelligence. In this way, the authors aim to explore the impact of writing review articles on strengthening these research skills among students. A mixed-methods approach was employed, beginning with a bibliometric stage that analyzed 679 articles published in Scopus between 2019 and 2023. The second stage focused on the thematic analysis of the most relevant research through the development of content units. It was identified that there is a marked interest in the scientific community regarding the study of integrating artificial intelligence into education, specifically in the writing of review articles as a pedagogical strategy to acquire writing skills. Specifically, the benefits of artificial intelligence in bibliometrics and linguistic and grammatical adaptations are highlighted. However, the need to adopt an ethical approach in the application of artificial intelligence is emphasized, so that university students can develop academic writing skills, thereby ensuring that these tools promote student development in a more responsible manner.

Professional improbity: How Hermann J. Muller's ethics affected his science.

The present paper provides an assessment of how the scientific and national policy achievements/goals of Hermann J. Muller were impacted by his ethics and provides several documented episodes in which Muller acted unethically to promote his personal gain-at the expense of others-within the scientific community. Muller manipulated the scientific community in self-serving ways to suppress perspectives that challenged his own views on radiation-induced gene mutation, and hereditary and cancer risk assessment in ways that influenced his significant awards (e.g., Nobel Prize in Medicine or Physiology), continued grant funding, and manifest effect on public health policy. Muller acted irresponsibly toward students and directed them to violate University of Texas policies that incurred severe student disciplinary actions (e.g., University suspension). Muller avoided responsibility by resigning from the University of Texas, avoiding a trial that could have led to his dismissal, and impacted his career achievements during the period of his nomination for the Nobel Prize. Muller was also a member of a US National Academy of Sciences Committee that committed scientific misconduct by misrepresenting the research record in ways that enhanced his continued funding support and fortified his influence on US health policy. The case of Muller is presented as a morality and object lesson worthy of consideration for current and future ethical conduct of scientific research.

Applying the Aajiiqatigiingniq Research Methodology: approaches and lessons learned through collaborative survey development and analysis

Aajiiqatigiingniq is an Inuit Qaujimajatuqangit principle used to discuss serious topics and come to consensus around decision-making. In working with Elders on community research issues, the Aqqiumavvik Society in Arviat, Nunavut developed the Aajiiqatigiingniq Research Methodology (ARM) as a participatory and fully inclusive Inuit research approach. We followed the ARM in establishing partnerships, and developing and facilitating a survey, to learn how Nunavut community members use and share available weather, water, ice, and climate information. The ARM guided our 5-year research process involving 19 Local Research Coordinators, along with 13 Nunavut-based, eight university, and three federal government collaborators. Through community research leadership and connecting long-term partnerships, we worked in eight Nunavut communities. In this paper, we present the opportunities and challenges of putting the ARM into practice through four research stages: (1) building relationships; (2) building shared understanding; (3) knowledge sharing; and (4) collaborative analysis. In this process, we have come to recognize our strength in connection, Local Research Coordinators as Highly Qualified Personnel, the four Rs of collaborative analysis, and the challenges of hurdling institutions. We share our approach and lessons learned to contribute to ongoing efforts and dialogue around decolonizing research and enhancing Inuit self-determination in research.