Research Experiences For Students Research Articles

An Undergraduate Course in CRISPR/Cas9-Mediated Gene Editing in Zebrafish.

We have developed a one-credit semester-long research experience for undergraduate students that involves the use of CRISPR/Cas9 to edit genes in zebrafish. The course is available to students at all stages of their undergraduate training and can be taken up to four times. Students select a gene of interest to edit as the basis of their semester-long project. To select a gene, exploration of developmental processes and human disease is encouraged. As part of the course, students use basic bioinformatic tools, design guide RNAs, inject zebrafish embryos, and analyze both the molecular consequences of gene editing and phenotypic outcomes. Over the 10 years we have offered the course, enrollment has grown from less than 10 students to more than 60 students per semester. Each year, we choose a different gene editing strategy to explore based on recent publications of gene editing methodologies. These have included making CRISPants, targeted integrations, and large gene deletions. In this study, we present how we structure the course and our assessment of the course over the past 3 years.

An interdisciplinary course on computer-aided drug discovery to broaden student participation in original scientific research.

We present a new highly interdisciplinary project-based course in computer aided drug discovery (CADD). This course was developed in response to a call for alternative pedagogical approaches during the COVID-19 pandemic, which caused the cancellation of a face-to-face summer research program sponsored by the Louisiana Biomedical Research Network (LBRN). The course integrates guided research and educational experiences for chemistry, biology, and computer science students. We implement research-based methods with publicly available tools in bioinformatics and molecular modeling to identify and prioritize promising antiviral drug candidates for COVID-19. The purpose of this course is three-fold: I. Implement an active learning and inclusive pedagogy that fosters student engagement and research mindset; II. Develop student interdisciplinary research skills that are highly beneficial in a broader scientific context; III. Demonstrate that pedagogical shifts (initially incurred during the COVID-19 pandemic) can furnish longer-term instructional benefits. The course, which has now been successfully taught a total of five times, incorporates four modules, including lectures/discussions, live demos, inquiry-based assignments, and science communication.

Summer Cancer Research Experience for High School Students from Historically Marginalized Populations in Kansas City.

The Accelerate Cancer Education (ACE) summer research program at The University of Kansas Cancer Center (KUCC) is a six-week, cancer-focused, summer research experience for high school students from historically marginalized populations in the Kansas City metropolitan area. Cancer affects all populations and continues to be the second leading cause of death in the United States, and a large number of disparities impact racial and ethnic minorities, including increased cancer incidence and mortality. Critically, strategies to bolster diversity, equity, inclusion, and accessibility are needed to address persistent cancer disparities. The ACE program offers an educational opportunity for a population of students who otherwise would not have easy access onto a medical center campus to make connections with cancer physicians and researchers and provides a vital response to the need for a more diverse and expansive oncology workforce. Students grow their technical, social, and professional skills and develop self-efficacy and long-lasting connections that help them matriculate and persist through post-secondary education. Developed in 2018, the ACE program has trained 37 high school junior and senior students. This article describes the need for and how we successfully developed and implemented the ACE program.

Beyond the curriculum: unveiling medical students' drivers and barriers to research participation at Alfaisal University.

Medical education continually adapts to the needs of future health care professionals, with student motivation in research being a pivotal aspect. This study at Alfaisal University aimed to explore the motivations, benefits, and challenges faced by medical students in extracurricular research activities. Using a mixed-method approach, we combined quantitative surveys with qualitative group interviews. Findings revealed that both extrinsic (e.g., enhancing postgraduate training prospects) and intrinsic (e.g., personal interest and skill refinement) factors significantly motivate students to be involved in research activities. Participants unanimously acknowledged skill enhancement, particularly in literature comprehension, creative ideation, and networking. However, challenges such as conflicts with course scheduling, lack of hands-on experiences, and mentorship issues were identified as potential barriers to research participation. Addressing these barriers and understanding motivations can inform the design of research programs, enhancing the overall student research experience. This study underscores the importance of research in medical education, emphasizing the need for institutions to prioritize addressing challenges and leveraging benefits to prepare medical students for a research-integrated clinical future.NEW & NOTEWORTHY This article examines the motivating factors and obstacles of extracurricular research in Alfaisal University, Saudi Arabia. The study utilizes a mixed methodology of online surveys and in-person group interviews to gain insights from the medical students of the university. We revealed several extrinsic and intrinsic motivators that drove the students; however, there remain several challenges to students during their research journey. Addressing these challenges will help the students obtain a more fruitful, educational research experience.

YEvo: A modular eukaryotic genetics and evolution research experience for high school students.

The resources for carrying out and analyzing microbial evolution experiments have become more accessible, making it possible to expand these studies beyond the research laboratory and into the classroom. We developed five connected, standards-aligned yeast evolution laboratory modules, called "yEvo," for high school students. The modules enable students to take agency in answering open-ended research questions. In Module 1, students evolve baker's yeast to tolerate an antifungal drug, and in subsequent modules, investigate how evolved yeasts adapted to this stressful condition at both the phenotype and genotype levels. We used pre- and post-surveys from 72 students at two different schools and post-interviews with students and teachers to assess our program goals and guide module improvement over 3 years. We measured changes in student conceptions, confidence in scientific practices, and interest in STEM careers. Students who participated in yEvo showed improvements in understanding of activity-specific concepts and reported increased confidence in designing a valid biology experiment. Student experimental data replicated literature findings and has led to new insights into antifungal resistance. The modules and provided materials, alongside "proof of concept" evaluation metrics, will serve as a model for other university researchers and K - 16 classrooms interested in engaging in open-ended research questions using yeast as a model system.

A Model of Science, Technology, Engineering, and Mathematics Remote Research-Based Learning

Opportunities for research-based learning at the high school level are limited, and with the COVID-19 pandemic, these have been further reduced. Such opportunities are particularly scarce for authentic research experiences (AREs), which allow students to identify as scientists by collecting data that contributes to scientists’ research. In response to the COVID-19 pandemic, we adapted two of our AREs for classroom settings, as remote independent research experiences for students to conduct from home. User guides and protocols from the AREs, Genotype-to-Phenotype Research with Corn and Discover Volvox Development, were adapted to instruct high school students to work on their own with the guidance of scientists and ARE coordinators. These independent authentic research experiences (IAREs) were implemented in the summer of 2020 and have since been available to students. Student responses to reflection questions and the Laboratory Course Assessment Survey indicate that IAREs provide students with significant gains including learning science content and research practices, collaborating with scientists, facing and resolving challenges, and contributing to scientific research.

Fostering Knowledge to Innovation: Enhancing Student Research Experience in Transforming Ideas into Project, Patent and Product

Abstract—This research study focuses on fostering a transformative journey from knowledge to innovation among students by enhancing their research experiences. The process involves converting initial conceptual ideas into tangible projects, which are subsequently developed into design patents, culminating in the creation of market-ready products. The study draws inspiration from the exploration of the critical radius of insulation, undertaken during the sixth semester of a mechanical engineering undergraduate program. This concept was practically applied to mitigate heat losses in household pressure cookers. Notably, a gap in the availability of such products in the Indian market was identified through extensive literature review, leading to the student’s engagement in this project. Encouraged by their mentors, the students presented their innovation to the university innovation, incubation, and research center, successfully obtaining financial backing. This paper comprehensively recounts the entirety of the student’s research journey. It commences with the translation of theoretical curriculum knowledge into actionable research ideas. The subsequent phases encompass various research methodologies, including theoretical analysis and simulation studies. The journey progresses with the execution of experimental approaches, effectively translating findings into design patents and, ultimately, tangible market-ready products. Overall, this research emphasizes the crucial role of research experiences in bridging the gap between theoretical learning and practical application. It underscores the significance of nurturing student’s innovative capabilities within the structured curriculum, contributing to their holistic development as future contributors to academia and industry alike. Keywords—Idea; Energy; Patent; Research; Transformation.

International Research Experiences for Students (NSF)

International Research Experiences for Students (NSF)

"So, We Found a Way:" How Changing Modalities Affected a Year-Long Mentored Research Experience for Associate's Degree Students.

The CUNY Research Scholars Program (CRSP) has provided year-long mentored research experiences for 1678 associate's degree STEM students since 2014. The pluralities (32%) of mentors, all of whom are full-time faculty, have been biologists. Other represented disciplines include, but are not limited to, chemistry, engineering, mathematics, environmental science, linguistics, and psychology. The research experiences take place at all 10 associate's degree-granting colleges within the City University of New York system. Our previous assessment demonstrated that CRSP students are significantly more likely than their counterparts in a matched sample to remain in STEM programs, graduate, transfer to research intensive institutions, and report a stronger sense of belonging in college. The Covid-19 pandemic challenged the program, as colleges shuttered laboratories and other facilities. Some mentors worried that lab-based research experiences would not be possible under such conditions. The first full-year pandemic cohort, however, demonstrated the resilience of the program and its participants. To assess the ongoing impact of CRSP and how it adapted using new modalities, we interviewed college-based directors, surveyed students and mentors, and held focus groups with mentors. Directors described how their colleges adapted to preserve all prepandemic components of the program. Mentors detailed their strategies for engaging students in authentic research experiences in virtual and other formats. Students reported that, along with scientific and technical skills, the program deepened their self-confidence and prepared them for transfer to baccalaureate programs. Our findings show how virtual platforms can be utilized to preserve the most beneficial aspects of undergraduate research experiences for associate's degree students.

Development and scale-up of gluten-free sorghum-based bakery goods for K-state Dining Services

To create new product opportunities for sorghum in the state of Kansas, the FARMUs project had three primary pillars: Innovate, Scale, and Connect. The project aims to close the gap between industry and consumers by peaking interest in sorghum, its nutritional and sustainable benefits, and consumption of sorghum-based products. The primary focus of this study was to develop and scale-up three gluten-free sorghum-based products for Kansas State Dining Services. The secondary objective was to evaluate the sensory and physical properties of these products to provide developmental and research experience for students. Preliminary testing involved identifying optimum ratios of sorghum flour, xanthan gum, starch, and eggs for proper binding. Whipped egg whites were tested at different levels for product volume and texture. Repetition and reformulation of the products provided various renditions of each product. Quantity equipment needs, staffing feasibility, ingredient availability, and cost were determined. Dining staff evaluated each product and once recipes were finalized, parameters (color, specific gravity, internal temperature, moisture loss, height/width, water activity) were evaluated. Dining center customers provided acceptability feedback for the three gluten-free products using the 9-point Hedonic scale. The first product, a savory waffle, can be stacked with turkey sausage and cheddar cheese for a breakfast sandwich. It utilizes sorghum flour, egg whites, xanthan gum, starch, and flaxseed for structure. This product received a range of 7.03–7.63 (n = 43) for all sensory attributes (acceptability, flavor, mouthfeel, texture, and aftertaste). The lemon blueberry muffin contains sorghum flour, eggs, buttermilk, xanthan gum, potato starch, and egg whites. Sensory data (n = 49) measured at acceptability (7.4), flavor (7.6), mouthfeel (6.4), texture (6.6), and aftertaste (7.6). The third product, a sweet potato muffin top, contains sorghum flour, xanthan gum, eggs, egg whites, and sweet potatoes. All three recipes were formatted via Computrition Menu Management system to provide methodology, nutritional analysis, costing, and labeling for university dining use. These recipes, along with sorghum-based educational materials will be shared with higher learning facilities across Kansas.

Characterizing the research mentorship experience of genetic counseling students.

The Accreditation Council for Genetic Counseling's Practice-Based Competencies include research-related skills, which are taught in master's level genetic counseling programs through didactic coursework and completion of mentored research experiences. It is known that research mentors can impact student work environments, create positive perceptions of the research process, and increase students' likelihood of future involvement in research. However, few studies have characterized the experiences of GC students in receiving research mentorship. It is crucial to understand these experiences from student perspectives to better inform stakeholders about factors that impact mentorship. Using a mixed-methods approach, this study explored GC students' experiences receiving research mentorship and their thoughts regarding the successful qualities of research mentors. GC students (N = 165) who graduated between 2019 and 2022 responded to an online survey measuring the mentorship relationship, defined by the Advisory Working Alliance Inventory (AWAI). On average, participants scored 3.96/5 on the AWAI, where higher scores indicate stronger working alliances. When asked to describe their overall research experience in three words, 75.7% of participants used at least one negatively connotated descriptor. Thematic analysis of semi-structured interviews obtained via purposive sampling of highest and lowest scoring participants on the AWAI (N = 14) revealed the following five themes related to successful qualities of a research mentor: (1) communication; (2) rapport building and relationship; (3) engagement and guidance; (4) expertise and connections; and (5) mentors with different roles. Of note, many of these qualities are foundational skills in genetic counseling. Thus, genetic counselors who may be strong in these areas who do not identify as "researchers" ought to consider becoming a research committee member. Additionally, education programs could consider implementing research committee member evaluations and/or student research self-efficacy surveys to evaluate how these relationships may be shaping research experiences for students within their program.

Responsible and ethical conduct of research (RECR) diagnostic survey using case scenarios from biology course-based undergraduate research experiences (CUREs).

Course-based undergraduate research experiences (CUREs) are increasingly becoming the first, and perhaps only, research experience for many biology students. Responsible and ethical conduct of research (RECR) is crucial for the integrity of scientific research and essential for students to have an understanding of the scientific process at any academic level. However, there is a current lack of RECR education in biology CUREs. To understand the level of RECR knowledge and skills in undergraduate students, we created a diagnostic survey that uses case scenarios designed to illustrate RECR issues in the CURE classroom. Analysis of students' responses indicated that the overall percentage of students who are able to effectively use RECR terminology and identify the impact of RECR violations on science integrity and ultimately on society is low. Furthermore, some students equated RECR violations to academic dishonesty, indicating difficulties separating the research and academic aspects of CUREs. This diagnostic tool can aid instructors in identifying gaps in student RECR knowledge for the subsequent development of RECR educational interventions, particularly to ensure the integrity of the research performed in CURE settings.

Accelerating Research Careers in Science Through Early Mentored Research Experiences for Undergraduates and Masters' Students at the University of California, San Francisco Center for AIDS Research.

Investments early in the academic pathway are essential to increasing the diversity of the HIV research workforce. Applied mentored research experiences can advance research skills, self-efficacy, and retention in science among scholars considered to be underrepresented minorities. The UCSF Center for AIDS Research Scholars Program is a 16-week program that pairs URM undergraduate and masters-level students from San Francisco State University with URM investigators from the UCSF. The program includes one-on-one mentorship on an independent research project, a core-curriculum on research methods and HIV-specific topics, and clinical shadowing. We evaluated program outcomes and areas for improvement with preprogram and postprogram survey and qualitative data from scholars and mentors from June to October 2022. Outcomes included interest in HIV research and growth on a 20-item measure of research skills, knowledge, and confidence. Six URM scholars enrolled in the inaugural cohort and were paired with 6 mentors. Preprogram and postprogram evaluations showed gains in interest in HIV research, 0% preprogram and 100% of scholars postprogram reporting much or extensive interest in HIV research, and gains across all domains from self-confidence, skills, and clarification of a research career path. Qualitative findings noted the importance of peer support and interpersonal features of mentors, including treating them like equals. Areas for improvement included more opportunities for primary data collection and specific training around mentoring undergraduates for mentors. The UCSF Center for AIDS Research Scholars Program was successful in building foundational skills, knowledge, confidence, and interest in HIV research among URM undergraduates and masters-level students.

Student-Led Undergraduate Journals: A Catalyst for Comprehensive Research Experience and Professional Growth

This opinion piece underscores the critical role of undergraduate academic journals in fostering a comprehensive research experience for students, with a spotlight on Qapsule, an open-access journal at Queen’s University led by undergraduates. These journals offer a unique platform for students to engage in the full spectrum of scientific inquiry, from conducting research to writing, peer review, and publication. The paper emphasizes the importance of undergraduate students’ involvement in all aspects of research, discussing its crucial role in intellectual growth and professional development. However, these journals often remain under-utilized due to a lack of awareness. The paper discusses the importance of universities actively promoting these journals and providing the necessary resources for students to establish such platforms, thereby nurturing a culture of academic collaboration, creativity, and excellence. It also addresses quality concerns about undergraduate journals, asserting that with appropriate mentorship and guidance, undergraduate students are capable of contributing to the academic community.

“It Seems Like I’m Doing Something More Important”—An Interpretative Phenomenological Analysis of the Transformative Impact of Research Experiences for STEM Students with ADHD

Recent studies have suggested a link between attention deficit hyperactivity disorder (ADHD) and increased creativity and ingenuity. Clinical work suggests that individuals with ADHD generally gravitate toward exploratory rather than exploitative thinking. Yet, these theories have not been tested in the field. This paper is a case study of a transformative undergraduate summer research program that allows engineering students with ADHD to spend 10 weeks in engineering labs at a research-intensive (R1) university. The program intends to show that students with ADHD can excel in engineering and STEM fields when placed in research environments that align with their natural cognitive processes and preferences. Using an interpretative phenomenological analysis (IPA) on post-program participant interviews, this paper suggests that engineering students with ADHD perceive that they learn more effectively when given the opportunity to explore academic material via hands-on research. The traditional structure of STEM education, with its focus on lectures and rigid curricula, causes significant harm to these students, leading to struggles, anxiety, and even thoughts of dropping out. In contrast, the research environment appears to foster creativity and motivation in students with ADHD, as it allows for exploration, provides real-world problem-solving opportunities, and offers tangible, hands-on experiences. This paper highlights the need for a paradigm shift in engineering pedagogy to better engage with and retain this neurodiverse student population and fully harness their creative potential.

Mentoring Applications From Applied Plant Systems Research Experiences With Undergraduates

Research experiences for undergraduate (REU) students are valuable for exploring STEM professions. Students from underrepresented groups and non-research institutions may not have the opportunities to engage in hands-on research. A primary goal of our Applied Plant Systems REU was to provide authentic research experiences for students who may not have the chance. Mentoring is key to the success of a REU, yet intentional mentoring is not often prioritized. Over three summers, we explored student perception of the value of mentoring within an agricultural STEM REU. Pre- and post-survey responses were analyzed, and we found that the students valued specific aspects of mentoring during their REU. Interestingly, at the end of the REU, the survey result showed that the students placed a higher importance on items such as mentoring being a systematic process and that mentoring is based on friendship. There is clear indication that students know good mentoring when they experience it, even if it is not verbalized. Based on the survey responses and comments from students involved in this REU, we developed practical mentoring items which can be incorporated into research programs to enhance the mentoring experience.

Fish in a Dish: Using Zebrafish in Authentic Science Research Experiences for Under-represented High School Students from West Virginia.

Early research experiences positively affect students' interest in STEM careers, and develop practical science and critical thinking skills. However, outreach opportunities are not equally accessible for all students. In states like West Virginia, where many students live in rural Appalachian communities, opportunities for engaging in STEM experiences are limited. In addition, rural teachers may not be equipped to provide authentic research experiences for students due to lack of resources or support. For many students in West Virginia, the Health Sciences and Technology Academy (HSTA) is a major opportunity for STEM engagement. Since its inception in 1998, HSTA has spread to 26 of 55 counties in West Virginia. The program recruits first-generation, low-socioeconomic status, rurally living, and African American high school students who are under-represented in STEM fields. Our research laboratory partnered with HSTA to implement an innovative, hands-on research camp using zebrafish for students participating in their annual junior-level biomedical sciences summer camp. Our camp was held in-person and adapted to an online format during the Covid-19 pandemic. We used pre-post surveys in both camps to assess impacts on science confidence and to collect information about general perceptions of zebrafish, research, and STEM fields. We found that students participating in the in-person and online camps experienced similar overall gains in science confidence. We also identified strong interest in zebrafish, research, and STEM degrees among online students. Online students did not prefer virtual learning experiences; however, they still enjoyed our camp. We also surveyed high school teachers volunteering for HSTA to identify factors that would encourage use of zebrafish in classrooms. The most prominent needs include classroom supplies, experience, and funding. Our successful science-education partnership demonstrates that zebrafish research experiences foster positive outcomes for under-represented students, and can inform future outreach efforts and collaborations with teachers.

ExMASS: Implementation and evaluation of an authentic, inquiry-based research experience for secondary students

The Exploration of the Moon and Asteroids by Secondary Students (ExMASS) program provides pre-college students the opportunity to conduct authentic, inquiry-based research with assistance from their teacher and a professional scientist. This paper presents an overview of the ExMASS program and results of ongoing program evaluation. The goals of the ExMASS program are to 1) provide an opportunity for secondary students to engage in multiple practices of science, 2) foster positive student attitudes toward science, and 3) enhance student lunar and asteroid science content knowledge. Evaluation data affirms the program is meeting these stated goals. In particular, assessment of student attitudes toward science show statistically significant increases in positive attitudes post program. The validity of the survey has been shown previously through factor analysis (Shaner et al., 2018). This paper includes a discussion of the continued assessment of student attitudes toward science (n = 125 students), showing statistically significant changes in both personal connections to science and the importance of science in society.

Designing an international research experience for graduate students

As a result of the ongoing globalization, international student experiences are becoming fundamental to advance students’ development and knowledge. Even though such growing importance, the body of knowledge regarding international interventions is dominated by subjective accounts and lacks contrasted results. In order to design an international research experience for graduate students, recommendations on the design and content of such experiences based on the review of literature were explored. A qualitative and bibliographical analysis of 40 studies evaluating the influence of international student interventions informed about the variables that such interventions should measure and the impact of such experiences on student development. Researchers especially measured the competencies intercultural development (83%), intellectual growth (58%), personal development (58%), professional development (50%), and academic development (15%). However, most of the studies failed to develop an appropriate research design that enables researchers to capture the breadth and depth of an international intervention. Results include addressing various competencies that students can gain during their experience abroad, using mixed methods to collect the data, whereby data should be collected at different time points including before, and multiple times after the international experience by different means.

Principal's Undergraduate Research Leaders (PURLs)

The Principal's Undergraduate Research Leaders, or PURLs, serve a central role in facilitating research experiences for students through advising on research, and increasing the awareness of and participation in research. they also take a leadership role in supporting studnt-led research initiatives and engaging in knowledge mobilization. In 2023, there are six PURLs, all of whom will join this panel to talk about their experiences with the program, and to provide advice to any undergraduates who may wish to apply to the program in the future.
 For more information about the program and to apply please see Principal's Undergraduate Research Leaders.