Wavefront coding technology (WFC) is a computational photography technique that significantly extends a system's depth of field (DOF) while mitigating the impact of aberrations and the environment. A freely inserted wavefront coding microscopy system that realizes high-resolution imaging with large DOF in a wide field of view (FOV) is proposed. On the basis of a designed large DOF microscopy system, an adaptive genetic algorithm is developed to interactively analyze, design, and optimize the phase mask and its effects on the system. The algorithm adaptively adjusts the parameters for selection, crossover, and mutation. Furthermore, it reconstructs the optimization function while considering processability, thereby efficiently and accurately selecting phase mask parameters. The phase mask is prepared and integrated into the system with a freely inserted fixture, which provides flexibility and ease of operation. Wavefront encoding/decoding experiments demonstrate that the system can extend the DOF by approximately ±13 times in a large FOV, while slightly decreasing the resolution from 6.20 μm to 7.81 μm, and mitigating aberrations and FOV effects. The integrated phase mask does not influence the design of the optical system, is universal, and simplifies the system's structure. This provides advantages in applications such as biological microscopes, endoscopes, and drones, which have strict sample and environmental requirements.
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