Reproductive Status Research Articles

Prevalence and risk factors of bovine tuberculosis in cattle in selected districts of Fafan pastoral settings, Eastern Ethiopia

Cross-sectional study was conducted from October 2021 to August 2022 to investigate the prevalence and associated risk factors of bovine tuberculosis in cattle in selected districts of the pastoral settings of Fafan zone, Somali region, eastern Ethiopia. A comparative intradermal tuberculin test was performed using purified protein derivatives. Animal-related characteristics, and the owner's knowledge on the importance of BTB were collected using a structured questionnaire. The prevalence was 11.24 % (95 % CI, 8.61–14.35) and 43.3 % (95 % CI, 33.27–53.75) at the individual and herd levels, respectively. There were statistically significant differences in the proportions of positive reactor animals according to body condition score (P = 0.000), age (P = 0.048), seasonal migration (P = 0.038), parity number (P = 0.005), and reproductive status (P = 0.037). Animals with poor body condition scores had a significantly higher likelihood of testing positive, with their odds being 11.4 times greater (COR = 11.408, CI = 3.43–37.94, P < 0.001). In multivariate logistic regression, poor body condition score remained significantly associated with the odds of a positive reaction to tuberculosis (AOR = 0.137, CI = 0.053–0.356, P < 0.001). Similarly, the analysis showed that seasonal migration (AOR = 2.882, CI = 1.155–7.191, P = 0.023) and parity number (AOR = 11.64, CI = 1.818–74.464, P = 0.010) were significant predictors of bovine tuberculosis infection in cattle. According to the questionnaire, 14.2 % (17 of 120) and 13.3 % (16 of 120) of the respondents were knowledgeable about bovine tuberculosis and its transmission from animals to humans, and vice versa, respectively. The general judgment of herders' understanding of bovine tuberculosis transmission methods to humans was very low. The study findings showed a high prevalence of bovine tuberculosis in the study area, emphasizing the need for an effective control and prevention strategy.

Giant honeybees (Apis dorsata) trade off defensiveness against periodic mass flight activity.

The giant honeybee Apis dorsata (Fabricius, 1793) is an evolutionarily ancient species that builds its nests in the open. The nest consists of a single honeycomb covered with the bee curtain which are several layers of worker bees that remain almost motionless with their heads up and abdomens down on the nest surface, except for the mouth area, the hub between inner- and outer-nest activities. A colony may change this semi-quiescence several times a day, depending on its reproductive state and ambient temperature, to enter the state of mass flight activity (MFA), in which nest organisation is restructured and defense ability is likely to be suppressed (predicted by the mass-flight-suspend-defensiveness hypothesis). For this study, three episode of MFA (mfa1-3) of a selected experimental nest were analysed in a case study with sequences of >60 000 images at 50 Hz, each comprise a short pre-MFA session, the MFA and the post-MFA phase of further 10 min. To test colony defensiveness under normative conditions, a dummy wasp was cyclically presented with a standardised motion programme (Pd) with intervening sessions without such a presentation (nPd). Motion activity at five selected surveillance zones (sz1-5) on the nest were analysed. In contrast to mfa1,2, in mfa3 the experimental regime started with the cyclic presentation of the dummy wasp only after the MFA had subsided. As a result, the MFA intensity in mfa3 was significantly lower than in mfa1-2, suggesting that a colony is able to perceive external threats during the MFA. Characteristic ripples appear in the motion profiles, which can be interpreted as a start signal for the transition to MFA. Because they are strongest in the mouth zone and shift to higher frequencies on their way to the nest periphery, it can be concluded that MFA starts earlier in the mouth zone than in the peripheral zones, also suggesting that the mouth zone is a control centre for the scheduling of MFA. In Pd phases of pre- and postMFA, the histogram-based motion spectra are biphasic, suggesting two cohorts in the process, one remaining at quiescence and the other involved in shimmering. Under MFA, nPd and Pd spectra were typically Gaussian, suggesting that the nest mates with a uniform workload shifted to higher motion activity. At the end of the MFA, the spectra shift back to the lower motion activities and the Pd spectra form a biphasic again. This happens a few minutes earlier in the peripheral zones than in the mouth zone. Using time profiles of the skewness of the Pd motion spectra, the mass-flight-suspend-defensiveness hypothesis is confirmed, whereby the inhibition of defense ability was found to increase progressively during the MFA. These sawtooth-like time profiles of skewness during MFA show that defense capability is recovered again quite quickly at the end of MFA. Finally, with the help of the Pd motion spectra, clear indications can be obtained that the giant honeybees engage in a decision in the sense of a tradeoff between MFA and collective defensiveness, especially in the regions in the periphery to the mouth zone.

Virus-induced host cell metabolic alteration.

Viruses change the host cell metabolism to produce infectious particles and create optimal conditions for replication and reproduction. Numerous host cell pathways have been modified to ensure available biomolecules and sufficient energy. Metabolomics studies conducted over the past decade have revealed that eukaryotic viruses alter the metabolism of their host cells on a large scale. Modifying pathways like glycolysis, fatty acid synthesis and glutaminolysis could provide potential energy for virus multiplication. Thus, almost every virus has a unique metabolic signature and a different relationship between the viral life cycle and the individual metabolic processes. There are enormous research in virus induced metabolic reprogramming of host cells that is being conducted through numerous approaches using different vaccine candidates and antiviral drug substances. This review provides an overview of viral interference to different metabolic pathways and improved monitoring in this area will open up new ways for more effective antiviral therapies and combating virus induced oncogenesis.

A study of the effect of number of children on depression among rural older women: empirical evidence from China.

The association between early reproductive events and health status in later life has always been of interest across disciplines. The purpose of this study was to investigate whether there was an association between the number of children born in the early years of elderly women and their depression in later life based on a sample of older women aged 65 years and above with at least one child in rural China. Data from the Chinese Longitudinal Healthy Longevity Survey in 2018, this study used the ordinary least square method to conduct empirical research. This study has found a significant correlation between an increase in the number of children and depression in older rural women. When considering the sex of the child, the number of daughters had a greater and more significant impact on depression. Number of children may exacerbate depression of older women through declining self-rated health and reduced social activity, while increased inter-generational support alleviated depression. The association between number of children born and depression also existed in urban older women, though not significant. Therefore, it is suggested to accelerate the improvement of supporting policies related to childbirth, developing a healthy and scientific fertility culture, and improving rural maternal and child health services. Women should be assisted in balancing their roles in the family and in society, and in particular in sharing the burden of caring for children. Targeted efforts to increase old-age protection for older people.

Labour resources for farms

The article is devoted to the study of the problem of social conditions of reproduction of labour resources for farms in modern Russia. Based on the analysis of modern publications of the science-intensive Scopus database, the international experience of the development of farming and labour resources of this category of production organizations is considered. Significant substantive trends in the theoretical part of the study were reflected in the methodology and methods of empirical research conducted by the survey method in the South of Russia (Stavropol Krai). In total, 417 people permanently residing in rural areas of Stavropol Krai took part in the survey. Factor analysis of the survey data made it possible to develop a structural model for choosing living conditions in rural areas as a basic basis for the possible inclusion of the able-bodied population in farming activities. The structural elements of the model are: social guarantees at the federal and regional levels and their implementation at a high quality level, the authorities' concern for the well-being of citizens; favourable socio-ecosystem characteristics of in rural areas associated with high-quality nutrition, the possibility of creativity, preservation of folk traditions, manifestations of social initiative; the possibility of a healthy lifestyle of a modern young family with children in an ecologically clean social communications perceived as a safe habitat; inclusion of rural areas in regional socio–cultural and innovative processes; indigenous living – love for a small homeland. The obtained factor model will help in the development of programs for the effective involvement of new participants in the social group of farming and the development of effective state and regional policies in the field of reproduction of labour resources in rural areas of Russia.

Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population.

Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is a widely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiological information without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate a non-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe) and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through the gestation period (~12months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormones was detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of the gestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal samples were collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation and at the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% of the population; at the end of gestation, only 20-40% of the females had confirmed pregnancies. Our results demonstrated that the polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted through feces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, the findings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Although some abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestational process may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductive physiology of one of the most emblematic ungulates in South America.

Twenty-four-year investigation of the reproductive biology of Glis glis in England (Rodentia: Gliridae)

Nest boxes were used to monitor a population of edible dormice (Glis glis) for 24 years. Details were recorded of reproductive condition, age and co-habitation by ca. 10,000 tagged individuals. Age at first breeding, periodic failure to breed, ‘absenteeism’ among adults, changing litter size with growth of young, survival rates and lifetime reproductive output were documented. The proportion of communal breeders and their kinship was examined as well as the occurrence of breeding by subadults. Life strategy and potential benefits of seasonal and annual behaviour are discussed.

SEROLOGICAL SCREENING FOR Neospora caninum INFECTION IN A DAIRY CATTLE FARM WITH REPRODUCTIVE DISORDERS, IN SOUTH-EASTERN ROMANIA

Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67–37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32 - 58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature.

The power of denial. From denial to creation: creative resistance in the city of Athens

The crisis leads to multiple denials in the face of everyday life and the conditions of reproduction of work it imposes. In this paper, I describe the basic denials in spaces of creative resistance in Athens during multiple crises. Through a systematic study of collaborative economy spaces, solidarity structures, and independent art spaces, I discern a progression of individuals from denial to creation and ultimately to action. I also strive to articulate the emerging modes of mobilization within these spaces. Faced with this reality, the act of denial emerges as the primary and transformative catalyst, sparking the forces of creativity and resistance. The denials of everyday life imposed by neoliberalism, as witnessed in Athens during the crisis, resulted in the creation of autonomous and self-organized spaces.

Prognostic value of diagnostic criteria for familial hypercholesterolemia in men and women with dyslipidemia

Aim. To analyze the prognostic value of the Dutch Lipid Clinic Network (DLCN) and Simon Broome Register (SBR) screening criteria for familial hypercholesterolemia (FH) in men and women with dyslipidemia.Material and methods. The study included 1233 patients with dyslipidemia.Biomaterial samples from 421 patients were studied using Next Generation Sequencing (NGS) to identify FH-associated genes. Statistical analysis was carried out using the StatTech v program. 3.1.6 (OOO Stattekh, Russia).Results. The most significant factors for predicting the FH in men are the level of low-density lipoprotein cholesterol (LDL-C) (1,77 times), positive family history for coronary artery disease (CAD) (6,26 times), multivessel coronary artery disease (4,05 times); in women before menopause — LDL-C (1,77 times per 1 mmol/l) and/or family history of coronary artery disease (3,31 times), in menopausal women — total cholesterol level (1,79 times per 1 mmol/l), coronary artery disease (6,52 times) and/or family history of stroke (6,04 times), xanthomas (4,24 times). Acute myocardial infarction and/or coronary stenting, arcus senilis, extracranial artery atherosclerosis did not prove to be prognostically significant for FH diagnosis.Conclusion. Diagnostic criteria for potential FH vary among patient populations. Its diagnostic significance depends on sex, and in women, reproductive status.

Hydrological conditions of phytophilic fish reproduction in the Lower Don River under the influence of climate change and flow regulation

Hydrological conditions of phytophilic fish reproduction in the Lower Don River under the influence of climate change and flow regulation

Resolution of the First Ukrainian endometriosis forum “Drug treatment for endometriosis-associated pelvic pain. The quality of a woman’s life”

Endometriosis affects 10% of all women of reproductive age, i.e. 190 million patients. Ukraine currently does not gather statistics on the incidence of endometriosis, but, at a rough estimate, this number is approximately 280 thousand women and the real number may be higher. Endometriosis has known significant social, medical and economic impact. Therefore, Ukrainian leading specialists in obstetrics and gynecology focus on new treatments for endometriosis, given current scientific data and the needs of patients. The given resolution and algorithms for treatment for endometriosis are the common stand of leading specialists in gynecology in our country and were developed on the basis of modern domestic and foreign data and current clinical guidelines. This resolution aims to improve clinical approaches to the routine practice of treatment for endometriosis. The constant increase in the incidence of endometriosis and its management characteristics allows to conduct an extensive study of the effectiveness and safety profile of various progestogens. In recent years, a number of further evidence have appeared regarding dydrogesterone treatment for endometriosis. New data on the effectiveness of dydrogesterone offer another hormone-based treatment for endometriosis, which is extremely important given the need for long-term and individualized treatment for endometriosis.The Europ ean Society of Human Reproduction and Embryology (ESHRE) states that the degree of pain relief in endometriosis is the same for all hormone-based treatments, but the safety and tolerability profiles of different medicinal products differ, which must be considered. According to the obtained data on the effectiveness and metabolic safety profile of various progestogens and during the discussion, algorithms for dydrogesterone-based treatment for endometriosis were proposed.

Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls.

The objective of the present study was to determine the effects that the reproductive season has on the motility, kinematics, morphology, and sperm morphometry of Brahman bulls evaluated with a commercial CASA system. The experiment was carried out at the Costa Rica Institute of Technology from March to August 2021. A total of eight Brahman bulls were used. A total of 28 ejaculates were collected in the pre-mating period (PMP), during it (DMP), and after it (AMP) using an electroejaculator. The sperm concentration was measured with the Accuread photometer. The motility was measured using a Spermtrack® counting chamber. The analyses were performed with the CASA-Mot ISAS®v1 system. The morphology was analyzed using a microscope with a negative phase contrast objective. Morphometry was evaluated with the CASA-Morph. The sperm concentration did not present differences between the PMP and AMP; however, it was significantly higher than DMP (p > 0.05). Regarding the progressiveness variables, linearity on forward progression (LIN), straightness (STR), and wobble (WOB) were higher (p < 0.05) DMP. A kinematic principal component analysis grouped all the variables into three factors and an effect on the reproductive period was found (p < 0.05) in the parameters of the head and middle part of the sperm, such as width and perimeter, which were greater in the PMP. The length of the sperm head in the PMP and DMP did not show differences; however, both were larger (p < 0.05) than AMP. The insertion distance of the middle piece of the sperm was significantly greater than DMP. Finally, the PMP contained cells with a larger insertion angle (p < 0.05) than AMP. These findings are important to understand the implications of reproductive status on sperm quality and to consider them in andrological evaluations.

Reflexive Organization and Radicalized Modernity: Revisiting the Organization–Society Nexus

Organizations and societies have changed. Bureaucracy, as modernity’s form of organization, has transformed into a reflexive organization, coordinating the conditions of system reproduction in time–space with increased reflexivity. Simultaneously, modernity with capitalism, industrialization, and rationalization as its institutional dimensions radicalized; reflexive organizations as one of its driving forces and the reflexive organization as the new organization model. Today, reflexive organizations are the most ubiquitous and powerful agents in our societies, separating and integrating activities, practices, and occurrences in new time–space arrangements and sets of social systems together with others, fueling modernity’s dynamism and global scope and letting our societies resemble an engine without a driver, erratically running in directions we cannot foresee. To identify and understand these changes and their impact on our current world, we develop a research framework informed by Anthony Giddens’ structuration theory that upgrades social systems to the core of structuration theory and adds the reflexive mode of organization and a strategic perspective to Giddens’ theory of modernity.

Eco‐physiological arguments on the functional impact of Lepus timidus mitochondrial DNA introgression in Iberian hares (Lepus granatensis)

AbstractGenetic exchanges between species provide valuable opportunities to dissect the relative importance of neutral and selective processes driving introgression events and to test their functional relevance. In the northern half of the Iberian Peninsula, the Iberian hare, Lepus granatensis, presents extensive patterns of mitochondrial DNA (mtDNA) introgression from the mountain hare, L. timidus, a species that disappeared from the region after the Last Glacial Maximum. Here, we test whether mtDNA introgression affects reproductive success and body condition of Iberian hare individuals. For that end, hares (n = 149) were sampled from populations along the mtDNA introgression gradient during the 2013–2014 and 2014–2015 hunting seasons. Reproductive performance indicators, including pregnancy status and testicular weight, were recorded, while body condition was measured as kidney fat (our response variables). Predictors included individual traits (sex and age), sampling period (month and hunting season), mtDNA introgression, occurrence or burden of different endoparasites and environmental factors. Our results showed that mtDNA introgression was negatively associated with pregnancy in females and body condition in males, while a positive association was found with body condition in females. These results suggest that carrying the mountain hare mtDNA has physiological effects in Iberian hares, but that the allospecific mtDNA type may not always be beneficial for the individual. This study shows that the patterns of mtDNA introgression seen in the Iberian hare today may result from a complex balance between deleterious and beneficial individual effects.

Decline in Size-at-Maturity of European Hake in Relation to Environmental Regimes: A Case in the Eastern Ionian Sea.

European hake, Merluccius merluccius L. 1758, is a highly valuable demersal fish species exploited in both the east Atlantic and the Mediterranean Sea. Changes in the size-at-maturity of this species have been reported in various geographic areas. Size-at-maturity is a key parameter in fishery management. Our main goal was to study the trend of the size-at-maturity of European hake in the eastern Ionian Sea (Central Mediterranean) over the last five decades. Utilizing a multi-decadal series of data for various environmental variables, we employed multivariate analyses and non-additive modeling in an attempt to identify shifts in the climatic environment of the eastern Ionian Sea and whether the maturation of the hake population could be affected by these changes. The analyses used suggest a plausible environmental regime shift in the study area in the late 1990s/early 2000s. The decrease in size-at-maturity that was detected in the last two decades may, thus, be associated with environmental changes. However, as many fish stocks already experience fishery-induced evolution, further investigation is necessary to determine whether this environmental effect is an additional stressor on a possibly already fishery-impacted population. The outcomes of this study highlight the importance of investigating the relationship between fish reproductive traits and altered environmental conditions, as the latter are generally ignored during assessments, affecting the robustness of fishery management.

EVALUATION OF THIAMINE AND RIBOFLAVIN CONTENT IN FEED AND BVD

The need for the content of vitamins in compound feed are decisive elements that significantly affect the development of the animal's body, their metabolism, reproduction and general condition. Taking into account the nature of vitamins and depending on the ability to dissolve, vitamins are divided into main groups, these are those that dissolve in fats (A, D, etc.), and those that dissolve in water, group B. Adding vitamins to compound feed during fattening animals is difficult to overestimate, because the body, with the exception of vitamins D and B1, does not have the ability to synthesize them, and the lack of vitamins is the cause of various complications and disorders of physiological indicators of animals. Cereal plants are the supplier of almost all representatives of B vitamins (except B12). The content of group B vitamins in cereal crops, which are widely used in feed production, depends on such factors as genotype, methods of processing before the production of compound feed, storage conditions, and others.The quantitative determination of vitamins and the use of modern methods for the formation of a balance of vitamins in compound feed and BVD is an urgent task of manufacturers engaged in the production of compound feed for animals and poultry.

Investigation on reproductive health status of women workers in different positions in oilfield enterprises

Objective: The status and influencing factors of reproductive health (including menstrual period and gynecological diseases) of female workers in different positions of oilfield enterprises were analyzed. Methods: From January to December 2020, a total of 979 female workers in an oil field were selected as research objects by the judgment sampling method, and the "Female Reproductive Health Survey" was used as the investigation tool to investigate the demographic characteristics, menstrual status and gynecological diseases. The influential factors were analyzed by 2-test and logistic regression analysis. Results: The prevalence of abnormal menstruation was 26.1% (256/979), dysmenorrhea 53.1% (520/979), and gynecological diseases 54.34% (532/979). The prevalence of breast disease was 23.39% (229/979), uterine disease 11.03% (108/979), cervical disease 10.32% (101/979), and HPV infection 7.97% (78/979). Age, the nature of the job and whether occupational harmful factors were clear were the influencing factors of gynecological diseases (P=0.001, 0.000, 0.007). Age, job nature, working hours and work intensity were the influencing factors of abnormal menstruation (P=0.005, 0.000, 0.000, 0.010) . Conclusion: The reproductive health status of female workers in different positions of oil field enterprises is not optimistic, and the reproductive health status of female workers in professional and technical positions needs to be improved.

Availability of mates and food influence the plasticity in strategies mediating life‐history tradeoffs in an insect

Different life‐history strategies can be employed to navigate the tradeoff between investment into reproductive and somatic tissues, and plasticity in life‐history strategies may be one of animals' most important tools to counter ongoing global change. Therefore, we factorially manipulated the availability of two critical resources – mates and food – to determine plasticity in life‐history investment strategies. We tested several hypotheses using females of the variable field cricket Gryllus lineaticeps because it exhibits a wing dimorphism that mediates a tradeoff between investment into ovary mass and dispersal (flight capacity) during early adulthood – long‐winged females' investment into flight muscle obligates reduced ovaries, while short‐winged females lack flight but instead heavily invest into ovaries. The availability of food and mates played different roles in the plasticity of life‐history strategies. Song exposure as a proxy for mate density exerted its strongest (negative) effects on somatic tissue and flight capacity. Meanwhile, food availability strongly influenced food intake, which exerted its strongest (positive) effects on reproduction. Raw traits of reproductive and somatic investment, such as ovary and non‐ovary mass gained, respectively, positively covaried; yet, reproduction‐soma relationships disappeared or became negative (i.e. characterizing tradeoffs) when resource (food) acquisition was accounted for. Thus, failure to account for food intake can lead to misdiagnoses of the plasticity of reproduction‐related tradeoffs. Further, the negative effect of flight capacity on ovary mass gain was dependent on male song (acoustic × flight interaction) and food availability (food × flight interaction). These were likely adaptive responses because they allowed flight‐capable females to invest heavily into reproduction when conditions for reproduction were favorable (i.e. abundance of food and mates). In sum, we uncovered multi‐directional effects among dispersal capacity, resource availability, and the plasticity of the tradeoff between investment into reproductive and non‐reproductive tissues.

Formulation of Underage Marriage in The Perspective of Islamic Law and Legal Sociology

The child's independence in pursuing ideals and realizing success for the future is a desire for parents and the country. This can be achieved when the child has carried out a good learning process that can be taken through the formal or informal level. However, the irony that occurs if a child's dream of becoming a person is useful for the future, religion, and the state must be cut off with what happens, such as marriages that are still not on time or underage marriages. This research aims to analyze underage marriages from the perspective of Islamic law and understand the benchmarks of legal awareness in society to minimize the occurrence of underage marriages and to know the implications of underage marriages. This study is library research using a qualitative approach. The results show that Marriage is a strong bond or mitsqon golidhon carried out by couples who have met the age limit or baligh, but marriages, carried out by someone who is underage must be based on a recommendation through a marriage dispensation issued by a religious court. Second, community indicators in realizing legal knowledge and understanding will form a stable legal attitude so that they can apply patterns of legal behavior. Third, the implications of underage marriage include the disruption of compulsory education, Fulfillment of subsistence, which is not optimal for the families, diversion of responsibility to parents because they have not been able to work properly, Reproductive health conditions are still vulnerable due to lack of good nutrition and give rise to poverty and vulnerability to divorce.