Reproductive Phase Research Articles

Enhancing Crop Resilience to Climate Change through Biochar: A Review

Crop resilience is crucial in the face of climate change, as agricultural regions face unprecedented challenges such as rising global temperatures, altered precipitation patterns, and increased extreme weather events. These changes impact food security, crop yields, and the livelihoods of millions of farmers worldwide. Crops face threats from heat stress, changing pest and disease dynamics, water scarcity, and unpredictable growing seasons. Crop resilience involves a complex interplay of genetics, environmental factors, and agricultural practices. Researchers and agricultural scientists are exploring innovative approaches like selective breeding, genetic modification, and precision agriculture to enhance crop resilience. Integrating traditional knowledge and indigenous farming practices into modern agricultural strategies is essential for securing food production, ensuring the sustainability of agricultural systems, conserving biodiversity, and supporting community resilience in an uncertain climate future. Crop resilience is central to ensuring global food security, supporting rural livelihoods, preserving ecosystems, and advancing sustainable agriculture in the face of climate change challenges. Biochar, a climate-resilient agricultural amendment, is gaining attention for its role in enhancing agricultural sustainability and mitigating climate change. Its porous structure and high carbon content sequester carbon dioxide, improve soil health, and reduce nutrient leaching. Biochar's porous nature fosters a rich microbial community, aids in nutrient cycling, and aids in rehabilitating degraded soils. It also reduces synthetic fertilizer requirements and environmental pollution. Climate change significantly impacts crop agriculture, disrupting traditional growth patterns and threatening global food security. High temperatures cause heat stress, while droughts and floods cause soil desiccation, impairing crop yields. Increased plant diseases and pests spread, while higher CO2 levels stimulate photosynthesis but reduce essential nutrients. Rising temperatures disrupt vegetative and reproductive growth phases, pollination, and seed formation, compromising crop quality and market value. Biochar is a porous material formed through pyrolysis, a process where organic biomass is decomposed under limited oxygen conditions. It is primarily carbon-rich but contains hydrogen, oxygen, nitrogen, and minerals. As a soil amendment, biochar is a stable carbon sink with a high carbon content of 70-90%. Its porous structure allows it to efficiently adsorb and retain water, nutrients, and organic compounds. Its large surface area facilitates interactions with soil microbes and nutrient ions, and its high CEC helps in nutrient retention and soil fertility.

РОЛЬ МИКРОРНК В РАЗВИТИИ УСТОЙЧИВОСТИ РАСТЕНИЙ К ХОЛОДУ

Plants, as sessile organisms, are forced to adapt to changes in the environment, including temperature changes that limit growth, development and productivity). Abiotic stresses have become a serious problem due to their widespread nature and destructive effects on plants. However, plants have developed complex ways to perceive and respond to environmental changes. The mechanisms of plant resistance to hypothermia have been studied for a long time and transcriptional control of expression of genes sensitive to cold has been studied in some detail. To activate protective mechanisms, plants trigger a network of genetic regulators, including changing the expression of a significant part of genes using transcriptional and/or translational regulators. microRNAs can be considered important participants in the response of plants to cold stress. The key to understanding the involvement of microRNAs in plant stress reactions was studies that showed the regulation of their expression under stress. microRNAs are small non-coding RNAs that manifest themselves as important regulatory components in plants. microRNA expression includes transcription of microRNA genes by RNA polymerase II, multistage processing of primary transcripts using the enzyme DICER-LIKE1 (DCL1) and the formation of an effector complex consisting of miRNAs and proteins of the ARGONAUTE (AGO) family. Such complexes interact with complementary RNA targets, suppressing their expression at the transcriptional and post-transcriptional levels. Thus, microRNAs regulate a variety of biological processes, including responses to changes in the environment. Currently, microRNAs are considered as an important tool for regulating the work of genes. Such significant processes in plants as maintaining homeostasis, growth and development, transition from the vegetative to the reproductive phase, signaling and response to various stresses are regulated by microRNAs. The article describes the role of microRNAs in the response of plants to cold stress, analyzes microRNA targets, and discusses the prospects for using microRNAs in the practice of improving plant resistance to external influences.

Mechanical force of uterine occupation enables large vesicle extrusion from proteostressed maternal neurons.

Large vesicle extrusion from neurons may contribute to spreading pathogenic protein aggregates and promoting inflammatory responses, two mechanisms leading to neurodegenerative disease. Factors that regulate extrusion of large vesicles, such as exophers produced by proteostressed C. elegans touch neurons, are poorly understood. Here we document that mechanical force can significantly potentiate exopher extrusion from proteostressed neurons. Exopher production from the C. elegans ALMR neuron peaks at adult day 2 or 3, coinciding with the C. elegans reproductive peak. Genetic disruption of C. elegans germline, sperm, oocytes, or egg/early embryo production can strongly suppress exopher extrusion from the ALMR neurons during the peak period. Conversely, restoring egg production at the late reproductive phase through mating with males or inducing egg retention via genetic interventions that block egg-laying can strongly increase ALMR exopher production. Overall, genetic interventions that promote ALMR exopher production are associated with expanded uterus lengths and genetic interventions that suppress ALMR exopher production are associated with shorter uterus lengths. In addition to the impact of fertilized eggs, ALMR exopher production can be enhanced by filling the uterus with oocytes, dead eggs, or even fluid, supporting that distention consequences, rather than the presence of fertilized eggs, constitute the exopher-inducing stimulus. We conclude that the mechanical force of uterine occupation potentiates exopher extrusion from proximal proteostressed maternal neurons. Our observations draw attention to the potential importance of mechanical signaling in extracellular vesicle production and in aggregate spreading mechanisms, making a case for enhanced attention to mechanobiology in neurodegenerative disease.

Limited life‐history plasticity in marginal population of an invasive foundation species: Unraveling the genetic underpinnings and ecological implications

AbstractPlant's life history can evolve in response to variation in climate spatio‐temporally, but numerous multiple‐species studies overlook species‐specific (especially a foundation species) ecological effects and genetic underpinnings. For a species to successfully invade a region, likely to become a foundation species, life‐history variation of invasive plants exerts considerable ecological and evolutionary impacts on invaded ecosystems. We examined how an invasive foundation plant, Spartina alterniflora, varied in its life history along latitudinal gradient using a common gardens experiment. Two common gardens were located at range boundary in tropical zone and main distribution area of S. alterniflora in temperate zone in China. Within each population/garden, we measured the onset time of three successive phenological stages constituting the reproductive phase and a fitness trait. In the low‐latitude garden with higher temperature, we found that reproductive phase was advanced and its length prolonged compared to the high‐latitude garden. This could possibly due to lower plasticity of maturity time. Additionally, plasticity in the length of the reproductive phase positively related with fitness in the low‐latitude garden. Marginal population from tropic had the lowest plasticity and fitness, and the poor capacity to cope with changing environment may result in reduction of this population. These results reflected genetic divergence in life history of S. alterniflora in China. Our study provided a novel view to test the center–periphery hypothesis by integration across a plant's life history and highlighted the significance in considering evolution. Such insights can help us to understand long‐term ecological consequences of life‐history variation, with implications for plant fitness, species interaction, and ecosystem functions under climate change.

Alleviation of Different Climatic Conditions by Foliar Application of Salicylic Acid and Sodium Nitroprusside and Their Interactive Effects on Pigments and Sugar Content of Maize Under Different Sowing Dates

The agricultural sector is seriously impacted by climate change, leading to potential risks to food security. In terms of global food production, maize ranks third. As a result, crop production and food security depend critically on assessing the effects of climate change and developing measures to adapt maize. Regarding adaptability, changing planting dates and using different agrochemicals are more effective than other management. Crop models are part of a global decision support system to help farmers maximize yields despite unpredictable weather patterns. To mitigate yield loss and protect the ecosystem, it is essential to use efficient maize-sowing practices in the field. This experiment was carried out to identify the most favorable sowing dates that maximize yield while ensuring the crop’s productivity and the integrity of the surrounding ecosystem remain intact. The main aim of this experiment was to mitigate the different climatic conditions by exogenous application of salicylic acid (SA) and sodium nitroprusside (SNP) on pigments and sugar content in maize under different sowing dates. A field experiment was carried out in the School of Agriculture, Lovely Professional University, Punjab, India, during the spring season of 2022. The experiment dealt with various maize crops, PMH-10, sourced from the Punjab Agricultural University (PAU), Punjab. The experiment was conducted in an open-air environment. The experimental setup was laid out in a split-plot design. The results stated that foliar application of salicylic acid and sodium nitroprusside successfully influenced high-temperature tolerance and low temperature at the reproductive phase and initial vegetative stages with other growing climatic conditions of maize in early and late sowings when controlled by increasing the chlorophyll index, carotenoids content, and sugar content of maize.

Habitat type modulates sharp body mass oscillations in cyclic common vole populations

Cyclic rodent populations exhibit pronounced changes in body mass associated with the population cycle phase, long-known as Chitty effect. Although Chitty effect is a common epiphenomenon in both America and Europe, there is still incomplete evidence about the generality of these patterns across the entire range of most species. Moreover, despite decades of research, the underlying factors driving Chitty effect remains poorly understood. Here, we examined the influence of intrinsic and extrinsic factors that may underlie observed patterns in vole size variation in the Iberian common vole Microtus arvalis asturianus. We weighed and measured 2816 adult voles that were captured during 6 trapping periods. Vole numbers and body mass showed strong period- and phase-related variation both in females and males, demonstrating marked Chitty effect in the studied population. Body mass of adult males correlated with body length, evidencing that heavier males are also structurally larger. Statistical models showed that probability of occurrence of large-sized vole (> 37 g) was significantly more likely in reproductive males, during increase and peak phases, and it was modulated by habitat, with crop fields and field margins between crops showing an increased likelihood. We suggest an effect of the habitat on vole body mass mediated by predation.

Applications of forensic anthropology methodology: accuracy of virtual face reproductions performed on the Tenchini collection.

Facial reproduction validation uses the methodology typical of forensic anthropology to confirm the accuracy of three-dimensional reproductions of faces. Achieving high accuracy in virtual facial reproductions is still under study. In the present paper, the Tenchini collection, which contains wax reproductions of prisoners' faces and their skulls, was used. By creating facial reproductions on skulls from this collection, the result was compared with the real face of the person to whom the skull belongs. The three-dimensional volume of each examined skull of Tenchini collection was acquired via CT scan and photogrammetry. Subsequently, the virtual reproduction of each skull was processed using three-dimensional graphics software (ZBrush, Pixologic®). The morphological comparison parameters used in the field of personal identification by forensic anthropology were used. The blind procedure required the operator to see the mask subsequent to the facial reproduction phase, so that the facial reproduction cannot exploit the information that knowledge of the mask would have produced. The parameters used in the study of facial reproductions have shown discrepancies between the characteristics of the masks and the facial reproductions, partly expected because due to the lack of soft tissue on the skull. However, a high degree of accuracy in the facial reproductions performed due to the applied methodology was documented. The present study allowed us to observe how these parameters can be useful to study the accuracy of facial reproduction and identify what difficulties are encountered in producing a result close to the real appearance.

The influence of conspecific and heterospecific neighbours on avian reproductive success

Abstract We investigated the influence of conspecific and heterospecific neighbours on the reproductive success of mountain bluebirds (Sialia currucoides) and tree swallows (Tachycineta bicolor) over eight breeding seasons. The abundance of heterospecific neighbours was negatively associated with reproductive success in mountain bluebirds but positively associated with reproductive success in tree swallows during the early nesting period (i.e., hatching rate). For bluebirds, conspecific and heterospecific neighbour abundance was associated with higher reproductive success (i.e., fledging rate) during the later stages of the nesting period; the same was true for conspecific abundance for tree swallows. These findings could be explained by either positive behavioural interactions (e.g., shared defence) or by habitat quality. We found contrasting effects of nearest neighbour distance. For both mountain bluebirds and tree swallows, having a tree swallow neighbour in close proximity was positively associated with reproductive success during the early nesting period, while having a mountain bluebird neighbour in close proximity was negatively associated with reproductive success during the late nesting period for mountain bluebirds. Together, these results indicate that the effects of conspecific and heterospecific neighbours on reproductive success are species-specific and vary depending on the phase of reproduction.

Achieving stable and sustainable Mungbean yields in Australia via optimal sowing dates

ContextAs a rainfed, short-season crop, mungbean is a particularly attractive summer rotation option for Australian farmers, but the rate of mungbean adoption in Australia is low due to its high yield instability. A lack of research conducted on modern mungbean in Australia has hindered farmers’ ability to understand how to best manage the crop and close the yield gap. ObjectivesThe aim of this study was to provide recommendations for optimal sowing windows (balancing maximum yield potential with minimum risk—i.e. instability and crop failure). MethodsWe simulated 60 years of growing mungbean at 180 different sowing times, four different initial soil water levels and at seven sites across Queensland and New South Wales using the new Agricultural Production Systems sIMulator Next Generation (APSIM NextGen) Mungbean model. We then calculated the impact of sowing date on mungbean water use efficiency and on the soil water deficit for the subsequent crop in the rotation. ResultsHigher initial soil water levels decreased risk (instability and failure) at all sites. Arid sites with high evaporative demand had optimal sowing windows later (February) in the growing season. More temperate sites yielded better with higher levels of stability when sown between November and January. Sowing in September/October is not recommended in any site as it coincided with low yield stability and water use efficiency. Not all sites had a clear optimal sowing window. We conclude, therefore, that in some environments, sowing time can only marginally reduce risks in growing mungbean. ConclusionsWe identified optimal sowing window that can reduce risks of a failed crop by 20 % and increase stability without losing yield potential. The timing of the optimal sowing window for a given site appears to be influenced by avoiding periods with high evaporative demand during the reproductive phase. ImplicationsModelling has been critical for exploring the trade-offs between maximizing and stabilizing mungbean yields over a wide range of environments and growing seasons to determine the optimal sowing windows across the main growing environments in Australia.

Yield and Fruit Characteristics of Tomato Crops Grown with Mineral Macronutrients: Impact of Organo-Mineral Fertilizers through Foliar or Soil Applications.

The utilization of plant biostimulants has gained importance as a strategy by which to improve plant productivity and soil health. Two independent trials were conducted across two seasons (2021 and 2023) to evaluate the effects of foliar or soil applications of various commercial organo-mineral fertilizers (Futuroot®, Radicon® Amifort®) with biostimulant action that is exerted on yield and fruit characteristics of processing tomato crops (cv Taylor F1) that have been exposed to mineral macronutrients. These treatments were administered three times during the season: at the transplanting, pre-flowering and berry development stages. In the first trial, conducted in two fields characterized respectively by low and high fertility, foliar applications of Radicon®, which is based on humic acid and amino acids, increased the leaf greenness index SPAD compared with the control. Furthermore, the leaf green colour intensity (SPAD index), measured during the reproductive phases of the tomato exhibited a positive correlation (R2 = 0.726) with the marketable yield obtained. This increase in marketable yield was significant in the biostimulant treatment compared with the control in both soils, especially in the soil characterized by lower fertility (16.1%), when compared with the more fertile soil (6.8%). In the second trial, conducted in the low-fertility field mentioned above, soil applications of all biostimulants (Futuroot®, Radicon® and the combinations [Radicon® + Amifort-Plus®]) significantly increased the marketable yield by 27.8%, 13.5% and 27.7%, respectively, compared with the control. The most significant beneficial effects of both Futuroot® and [Radicon® + Amifort®] could be attributed to the combination of humic acids and auxins, cytokinins or microelements (Zn, Mn, MgO) present in the formulation of these products. Furthermore, the increase in marketable yield obtained when Radicon® was applied to leaves was higher (16.1%) than that observed with soil application (13.5%). In both trials, no relevant effects of biostimulant products were observed on most of the physicochemical characteristics of tomato fruits. In conclusion, the biostimulants based on humic acid and amino acids combined with chemical fertilizers tested in the present study and applied by fertigation were more effective in improving tomato yield, and therefore they can be recommended for efficient agricultural production.

Short-Term Reproduction of Active Movement with Visual Feedback and Passive Movement with a Therapist’s Hands

Reproducing instructed movements is crucial for practice in motor learning. In this study, we compared the short-term reproduction of active pelvis movements with visual feedback and passive movement with the therapist’s hands in an upright stance. Sixteen healthy males (M age = 34.1; SD = 10.2 years) participated in this study. In one condition, healthy males maintained an upright stance while a physical therapist moved the participant’s pelvis (passive movement instruction), and in a second condition, the participant actively moved their pelvis with visual feedback of the target and the online trajectory of the center of pressure (active movement instruction). Reproduction errors (displacement of the center of pressure in the medial–lateral axis) 10 s after the passive movement instruction were significantly greater than after the active movement instruction (p < 0.001), but this difference disappeared 30 s after the instruction (p = 0.118). Error of movement reproduction in the anterior–posterior axis after the passive movement instruction was significantly greater than after the active movement instruction, no matter how long the retention interval was between the instruction and reproduction phases (p = 0.025). Taken together, active pelvis movements with visual feedback, rather than passive movement with the therapist’s hand, is better to be used for instructing pelvis movements.

Cyclic Variations of Ovarian Development, Hormones and Sex Related Genes of Rainbow Trout (Oncorhynchus mykiss) During Different Growth Stages

This study aimed to investigate the reproductive biology of female rainbow trout (Oncorhynchus mykiss). Mature female (n.80) rainbow trout from different reproductive phases were collected. Through a transmission electron microscope (TEM), it was feasible to examine the oocyte at several developmental stages, namely the perinucleolus stage, cortical alveoli stage, vitellogenic stage, mature stage, and postovulatory stage. Progesterone and estradiol levels were measured at their lowest during the immature and spent phases, while reaching their highest levels during the maturing and mature stages. The total amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) was greater in mature ovaries compared to immature and maturing ovaries. Gene transcripts related to the production of steroids, specifically star, cyp11a1, cyp17a1 and hsd3b were expressed at low levels during the immature stage and showed a significant rise during the mature stage. The ovarian gene expression patterns during oogenesis exhibited distinct dissimilarities. The fatty acid content, essential for the development of fish ovaries, and the sex steroid hormonal profile, which play a key role in regulating oogenesis, are particularly significant. These results will enhance our comprehension of the reproductive biology and sustainable management of rainbow trout in their natural environment.

An improved crop calendar model for winter wheat incorporating vernalization and winter survival to project changes in phenology

Crop phenology and associated crop calendars are necessary for designing breeding goals and for developing effective management practices. Winter wheat is a representative biennial, the cultivation schedule of which is constrained by winter climate conditions, particularly the processes of vernalization and winter survival. Here, we present improvements to a rule-based crop calendar model by incorporating these factors so that it can be used to accurately estimate the phenological events of winter wheat from daily meteorological data. We tested the improved model in Hokkaido, the northernmost Japanese island, which is characterized by seasonal snow cover and a wet summer. The results confirmed that implementing these factors contributed to accurate estimates of peak occurrence dates of winter wheat phenological events. Furthermore, we applied the improved model to simulate wheat phenology under 2 K and 4 K warmer scenarios. The results showed a delayed sowing period up to approximately one month and slight advancements in both flowering and harvesting, leading to a shorter growth period. While this shortened period may be largely compensated by a decrease in the snow-covered period, the shifts in the vegetative and reproductive phases may modify sink-source balance of wheat. We also assessed the risks of pollination failure and preharvest sprouting, both of which are associated with the timing of precipitation, based on the number of rainy days around flowering, and the incidence of precipitation over two consecutive days around the time of harvesting. Our simulations suggested increased risk of pollination failure and reduced risk of preharvest sprouting, leading to an increase in the probability of crop failure. These findings underscore the importance of implementing adaptation measures to mitigate precipitation-related risk under future climates. Further, the findings provide valuable insights for winter wheat breeders and agronomists, thereby facilitating crop production adaptation.

Spiral Mechanism in <i>Arenga pinnata</i>

The phyllotaxy of Arenga pinnata is alternate and cyclic revealing two distinct foliar spirals. In some individuals, the two spirals veer to the left and in others, to the right. The number of leaflets on one half of leaf differs from that of the other. This difference is associated with the leaf spirals. Due to the monocarpic nature of the palm, its reproductive phase starts with a terminal inflorescence followed by the development of axillary inflorescences in basipetal order. The sex of the flowers varies with the position of the respective spadices on the palm. That is, the terminal and a few immediate successive spadices produce only female flowers, beyond which each inflorescence produces both female and male flowers, and the last many spadices have only males. The flowers are distributed in clusters of 2 males or 2 males and a female in between, or individually if they are only females. In a cluster of 2 or 3 flowers, The aestivation of sepals of the males varies with their position in the triad. The aestivation pattern in female flowers, however, has an influence on the spirality of the spika on which they are borne.

New PID parameter tuning based on improved dung beetle optimization algorithm

AbstractIn this paper, a proportional‐integral‐derivative (PID) controller parameter optimization method based on the improved dung beetle optimization (IDBO) algorithm is proposed, which improves the balance between the global exploration and local exploitation capabilities of the dung beetle optimization (DBO) and significantly enhances the convergence speed and optimization accuracy. Initially, the dung beetle population is initialized using piecewise linear chaotic map (PWLCM) chaotic mapping in order to increase its variety and the DBO algorithm's capacity for global exploration. Furthermore, adaptive weighting in the DBO algorithm is now balanced between the capabilities of local exploitation and global exploration with the addition of adaptive weights. After that, in order to improve the DBO algorithm's capacity for local exploitation, a triangle wandering strategy is included during the dung beetle reproductive phase. Finally, using both Lévy flying wandering and greedy strategy (GS) together make it easier to take advantage of opportunities in both local and global areas. The outcomes of the traditional benchmark function test demonstrate a significant improvement in both convergence speed and optimization accuracy when the particle swarm optimization (PSO), DBO, grey wolf optimization (GWO), and sparrow search algorithm (SSA) algorithms are compared. The performance index function incorporates an overshooting penalty term to prevent the overshooting phenomenon in the control system. Simulation experiments are carried out for the DC motor control system, and the time domain performance, frequency domain performance, and robustness performance of the closed‐loop control system with ZN‐PID, Lambda‐PID, PSO‐PID, and IDBO‐PID rectified PID controller parameters are comparatively analyzed, which verifies the validity and practicability of the IDBO algorithm.

Expression analyses of CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS in the one-leaf plant Monophyllaea glabra reveal neoteny evolution of shoot meristem

The one-leaf plant Monophyllaea glabra exhibits a unique developmental manner in which only one cotyledon continues growing without producing new vegetative organs. This morphology is formed by specific meristems, the groove meristem (GM) and the basal meristem (BM), which are thought to be modified shoot apical meristem (SAM) and leaf meristem. In this study, we analysed the expression of the organ boundary gene CUP-SHAPED COTYLEDON (CUC) and the SAM maintenance gene SHOOT MERISTEMLESS (STM) orthologs by whole-mount in situ hybridisation. We found that CUCs did not show clear border patterns around GM and BM during the vegetative phase. Furthermore, double-colour detection analysis at the cellular level revealed that CUC and STM expression overlapped in the GM region during the vegetative phase. We also found that this overlap is dissolved in the reproductive phase when normal shoot organogenesis is observed. Since co-expression of these genes occurs during SAM initiation under embryogenesis in Arabidopsis, our results demonstrate that GM is a prolonged stage of pre-mature SAM. Therefore, we propose that neotenic meristems could be a novel plant trait acquired by one-leaf plants.

Selection indices for high seed yield in desi chickpea (Cicer arietinum L.) genotypes under terminal heat stress

Seventy-one genotypes of desi chickpea including released varieties were evaluated over two years under normal and late conditions. The selection index based on discriminant function analysis was used to determine the phenotypic value of different characters in selection. Seed yield and its five contributing traits were used to construct the index under normal and late-sown conditions. The results indicated that the efficiency of the index increased with the inclusion of a greater number of traits. The best selection index for the normal sown condition was observed for three characters including date of maturity, reproductive phase duration, and number of pods per plant in E1 as well as with days to 50% flowering, days to maturity, and number of pods per plant in E3 . On the other hand, under late sown conditions (E2 and E4 ), four characters seed yield per plant, reproductive phase duration, number of pods per plant, and 100-seed weight had the best selection index. This study showed that the selection of these characters may be considered for better genetic gain for higher seed yield under any of these conditions.

Mitochondrial function in skeletal muscle contributes to reproductive endothermy in tegu lizards (Salvator merianae).

In cyclic climate variations, including seasonal changes, many animals regulate their energy demands to overcome critical transitory moments, restricting their high-demand activities to phases of resource abundance, enabling rapid growth and reproduction. Tegu lizards (Salvator merianae) are ectotherms with a robust annual cycle, being active during summer, hibernating during winter, and presenting a remarkable endothermy during reproduction in spring. Here, we evaluated whether changes in mitochondrial respiratory physiology in skeletal muscle could serve as a mechanism for the increased thermogenesis observed during the tegu's reproductive endothermy. We performed high-resolution respirometry and calorimetry in permeabilized red and white muscle fibers, sampled during summer (activity) and spring (high activity and reproduction), in association with citrate synthase measurements. During spring, the muscle fibers exhibited increased oxidative phosphorylation. They also enhanced uncoupled respiration and heat production via adenine nucleotide translocase (ANT), but not via uncoupling proteins (UCP). Citrate synthase activity was higher during the spring, suggesting greater mitochondrial density compared to the summer. These findings were consistent across both sexes and muscle types (red and white). The current results highlight potential cellular thermogenic mechanisms in an ectothermic reptile that contribute to transient endothermy. Our study indicates that the unique feature of transitioning to endothermy through nonshivering thermogenesis during the reproductive phase may be facilitated by higher mitochondrial density, function, and uncoupling within the skeletal muscle. This knowledge contributes significant elements to the broader picture of models for the evolution of endothermy, particularly in relation to the enhancement of aerobic capacity.

Unravelling some factors affecting sexual reproduction in rock-specialist shrub: Insight from an endemic Daphne arbuscula (Thymelaeaceae)

The role of endemic species in global biodiversity is pivotal, and understanding their biology and ecology is imperative for their fitness and long-term survival, particularly in the face of ongoing climatic oscillations. Our primary goal was to investigate the sexual reproduction level of the endangered Western Carpathian endemic Daphne arbuscula (Thymelaeaceae), which inhabits extreme rocky habitats, and to comprehend the influence of specific factors on its reproductive success. We conducted the research across four populations, varying in size and environmental conditions. Over two years, we monitored flower and fruit production, analyzed genetic variability within and among populations, and studied pollination mechanisms. Daphne arbuscula proved to be strictly self-incompatible, with significant variations in flower and fruit production among populations and seasons. The average fruit production percentage consistently remained below 50% across populations, indicating challenges in sexual reproduction. Cold and harsh weather during the reproductive phase had a substantial negative impact on sexual reproduction efficacy, leading to decreased fruit production. Nevertheless, several individuals in sheltered microhabitats displayed significantly higher fruit production, ranging from 60% to 83%, emphasizing the critical role of microhabitat heterogeneity in sustaining sexual reproduction in this species. We found no pronounced differences in genetic diversity within or among populations, suggesting that genetic factors may not critically influence the reproductive success of this endemic species. The implications of our findings might be of paramount importance for the long-term survival of D. arbuscula and offer valuable insights for the development of effective conservation strategies for this species.

Determination of Population Dynamics of Cicadellidae (Hemiptera) Species and Their Relationship with Climatic Parameters in Organic Cotton Fields in Hatay Province, Türkiye

In this study, population dynamics of leafhopper (Hemiptera: Cicadellidae) species in organic cotton-growing areas of Hatay province were investigated along with their associations with climatic parameters. Weekly sampling using a sweep net (100 sweep net/parcel) identified nine species within the Cicadellidae family. Among these, Asymmetrasca decedens (Paoli) and Empoasca decipiens (Paoli) displayed high population densities throughout the entire vegetation period of cotton, while Zyginidia sohrab (Zatchvakin), Psammotettix striatus (Linnaeus), Orosius orientalis (Matsumura), Anaceratagallia laevis (Ribaut), and Anaceratagallia sinuata (Mulsant & Rey) were present in both vegetative and reproductive phases of cotton, with Z. sohrab reaching significant population levels. Cicadulina bipunctella (Matsumura) and Balclutha hebe (Kirkaldy) were found only during the reproductive phase, yet they achieved considerably high populations. Correlation and regression analyses have shown a moderate to high level of positive correlation between the population development of five leafhopper species and various temperature (°C) parameters (r=0.578-0.790, p