Two types of cytoplasmic 17 beta-estradiol (E2) binding activity were identified and characterized in the uteri of pregnant, cycling and prepubertal, cycle-induced (400 IU pregnant mare's serum gonadotrophin (PMS) + 200 IU human chorionic gonadotrophin (hCG)) gilts. Overall, type I affinity and capacity were Kd 1.94 +/- 0.51 nM and 5.410 +/- 1.09 pmol/mg protein, respectively; type II apparent dissociation constant and capacity were Kd 21.34 +/- 6.83 nM and 62.58 +/- 15.96 pmol/mg protein, respectively. Cytoplasmic luteal E2 receptors were undetectable in all groups. Uterine E2 receptor activity was eluted from diethylaminoethyl columns by a 0.05-0.15 M KCl gradient. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated a molecular weight of 70 400-79 000. Excluding gilts with cystic ovarian follicles (16.67%), prepubertal gilts treated with PMS + hCG versus cycling sows had lower serum progesterone on days 6 and 9-13 of the estrous cycle and lower 13,14-dihydro-15-keto prostaglandin F2 alpha levels on days 0-9 and 13-17 of the cycle. Implants, containing 200 mg estrone inserted subcutaneously on days 12-19 after PMS + hCG treatment in gilts, had no discernible effects on these parameters. These results indicate that the diminished reproductive capacity of the gilt, in which cycle activity is induced by PMS + hCG, is likely due to decreased luteal progesterone secretion. Preliminary data also suggest that the lack of E2 receptors may contribute to the low reproductive performance in gilts with cystic ovarian follicles.
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