Representative Population Sample Research Articles

Prevalence and Associations of Xerostomia in Older Adults in Southern Brazil.

This study investigated the prevalence and associations of xerostomia in older adults in southern Brazil. A cross-sectional study was carried out in Pelotas, Brazil, during 2014. A representative sample of the city's older adult population (60+) was selected. The dependent variable was self-reported feeling of dry mouth in the past 6 months. Covariates included socio-economic status, schooling, gender, age, tobacco and alcohol consumption, polypharmacy, hypertension, diabetes, arthritis, depression, dentition status and use of removable dental prostheses. Descriptive analysis was carried out, and Poisson regression was used to obtain prevalence ratios and 95% confidence intervals. Analyses used STATA 15.1. Most of the 1451 participants were female (63.3%). The prevalence of xerostomia was 36.7% (95% CI 34.3-39.1). Adjusted analysis showed that xerostomia was significantly more common among females (PR 1.44, 95% CI 1.22-1.74), those with less schooling (PR 1.31, 95% CI 1.10-1.51), those exposed to polypharmacy (PR 1.22, 95% CI 1.05-1.37), people with arthritis (PR 1.42, 95% CI 1.23-1.61) and those with depression symptoms (PR 1.45, 95% CI 1.23-1.70). Xerostomia is common among older adults. Health workers need to pay attention to its associated factors for early identification and promotion of appropriate interventions, particularly the rational use of medicines.

Changes in life satisfaction over six years in the general population: A longitudinal study with the Satisfaction With Life Scale (SWLS).

Satisfaction with life is a key concept for most individuals. The Satisfaction With Life Scale (SWLS) for measuring general life satisfaction has been widely analyzed in terms of cross-sectional associations. However, the knowledge about long-term changes in life satisfaction and the associations between such changes and changes in other variables of physical and mental health is limited. A community-based representative sample of the general population has been examined twice with a time interval of six years (n = 4,999), using the SWLS and several other scales. Over the six years, the mean SWLS score of the total sample remained nearly unchanged (M = 27.0, SD = 5.2, both at t1 and at t2). The test-retest correlation was rtt = 0.66 for the total sample, and there were only marginal differences in temporal stability between male and female respondents. Changes in the SWLS over the six years were correlated with changes in optimism (r = 0.23), mental health (r = 0.26), social functioning (r = 0.22), perceived social support (r = 0.21), anxiety (r = -0.30), and physical complaints (r = -0.18). These change score correlations were lower than the corresponding coefficients under the cross-sectional perspective. Measurement invariance across sex, age, and time was established. The SWLS proved to be an appropriate tool for assessing changes in life satisfaction, and correlations between change scores of life satisfaction and health-related variables complement the knowledge about these associations from a cross-sectional perspective.

EXPLORING BARRIERS AND FACILITATORS TO PHYSICAL ACTIVITY IN EUROPEAN MIDLIFE AND OLDER ADULT SAMPLES

Abstract Physical activity (PA) is an adaptable and accessible health-promoting behavior with wide-ranging benefits (e.g., reduced risk for diabetes, heart disease, and all-cause mortality). However, only 24.3% of U.S. adults meet current recommendations for PA (150 minutes of moderate-intensity PA and 2 days of muscle strengthening activity per week). It is therefore crucial to understand factors that may support increased engagement in PA. The current study used a random forest machine learning analysis (RFA) alongside a conventional parametric methodology (i.e., linear multiple regression) to evaluate the relative importance of 58 risk/protective factors for PA (moderate and strenuous activity) in a population-representative sample of middle-aged and older adults (N = 67,603; 56.1% women; ages 45-105y; 18 countries) from the Survey of Health, Ageing and Retirement in Europe (SHARE Wave 6). For both women and men, top predictors identified through RFA included variables related to socio-demographics (age, sex, education, country), physical and functional health (mobility, self-rated health, grip strength), as well as variables related to mental health (cognitive performance, depressive symptoms) and socio-relational engagement (caring for others outside of the home, social connectedness). Follow-up hierarchical regressions showed that key predictors accounted for 35% of variability in PA, with contributions as follows: socio-demographic (20%), health/mobility (12%), and cognitive and socio-relational (3%). Together, these results indicate that PA is influenced by multiple bio-psycho-social factors and suggest that future interventions to promote PA may benefit by considering such constellations.

Evaluation of population immunity against SARS-CoV-2 variants, EG.5.1, FY.4, BA.2.86, JN.1, JN.1.4, and KP.3.1.1 using samples from two health demographic surveillance systems in Kenya

Increased immune evasion by emerging and highly mutated SARS-CoV-2 variants is a key challenge to the control of COVID-19. The majority of these mutations mainly target the spike protein, allowing the new variants to escape the immunity previously raised by vaccination and/or infection by earlier variants of SARS-CoV-2. In this study, we investigated the neutralizing capacity of antibodies against emerging variants of interest circulating between May 2023 and October 2024 using sera from representative samples of the Kenyan population. From our genomics data, we identified the most prevalent Kenyan and global variants and performed pseudoviruses neutralization assays with the most recent SARS-CoV-2 variants. Our data show that antibodies from individuals in the general population in Kenya were less effective against the recent prevalent SARS-CoV-2 omicron variants (i.e. EG.5.1, FY.4, BA.2.86, JN.1, JN.1.4, and KP.3.1.1) compared to the ancestral wildtype strain. Although there was increased neutralization following multiple doses of vaccine, antibodies from > 40% of the vaccinated individuals did not neutralize the omicron variants, suggesting that individuals were susceptible to infection by these variants.

Obsessive-Compulsive Symptoms in the General Population Under Stressful Conditions: Lessons Learned from the COVID-19 Pandemic.

Introduction: The COVID-19 pandemic had a negative impact on mental health in the general population. The fear, stress, and uncertainty surrounding that traumatic period could have contributed to the aggravation or possible new onset of obsessive-compulsive symptoms. Methods: The COvid Mental hEalth Trial (COMET) is a nationwide project organized by the University of Campania "Luigi Vanvitelli", designed as an observational investigation that aimed to gather data from a representative sample of the Italian general population. The current study is a report from the main project and it focuses on obsessive-compulsive (OC) symptoms. Results: A total sample of N = 20,720 took part in the survey. N = 2332 individuals had a total Obsessive-Compulsive Inventory-Revised (OCI-R) score greater than or equal to 21 (11.3% of the entire sample), indicating the presence of clinically relevant obsessive-compulsive symptoms. By excluding patients with a history of previous mental illnesses, we still obtained a high number of individuals with an OCI-R score greater than or equal to 21 (N = 2024), representing 10.3% of the overall sample, possibly indicating a new incidence of OC symptoms during the pandemic. Discussion: Our study highlights a substantial new incidence of obsessive-compulsive symptoms in the general public. Risk factors or red flags such as being male, being of working age, living in a highly stressful environment such as one of the Italian regions most affected and severely hit by the pandemic, having higher levels of loneliness, and using substances to cope with stress, should be paid particular attention in order to prevent the development of OC symptoms during a critical and traumatic event such as the COVID-19 pandemic.

The generic version of China Health Related Outcomes Measures (CHROME-G): psychometric testing and comparative performance with the EQ-5D-5L and SF-6Dv2 among the Chinese general population

ObjectivesThe CHROME-G is the first generic preference-based measure developed in China. This study aimed to validate and compare the psychometric properties of the CHROME-G with the EQ-5D-5L and SF-6Dv2 among the Chinese general population.MethodsA representative sample of the Chinese general population in terms of age, gender, education, and urban/rural residence was recruited for an online survey. During the survey, respondents completed three instruments (first the CHROME-G, then the EQ-5D-5L and SF-6Dv2 in random order), demographic and health-related questions. The retest survey was carried out after two weeks. Ceiling/floor effects were first assessed. Convergent and divergent validity was examined using Spearman’s rank correlation. Known-group validity was examined using the non-parametric Kruskal–Wallis H test and effect size. Test–retest reliability was assessed using the intraclass correlation coefficient and weighted Kappa statistics.ResultsOne thousand respondents (51.1% male, mean age 44.7 years) completed the first survey, with 378 also completing the retest survey. The mean ± SD completion time was 2.03 ± 0.58 min for the CHROME-G, and 1.37 ± 0.54 and 1.13 ± 0.38 min for the EQ-5D-5L and SF-6Dv2. Only the EQ-5D-5L had a ceiling effect of 35.1%. The range of Spearman rank’s correlations was 0.45–0.62 for convergent validity and 0.14–0.46 for divergent validity. Among different health subgroups, the effect size for the CHROME-G, EQ-5D-5L and SF-6Dv2 was 1.348–3.416, 1.362–3.325 and 1.097–2.228, respectively. The ICC for test–retest was 0.791 for the CHROME-G, compared with 0.994 and 0.971 for the EQ-5D-5L and SF-6Dv2.ConclusionsThe CHROME-G showed good and comparable psychometric properties with the EQ-5D-5L and SF-6Dv2.

Association between fine particulate matter (PM2.5) and violence cases in South Korea: A nationwide time-stratified care-crossover study.

Several studies reported the roles of short-term exposure to fine particulate matter (PM2.5) on violent behaviors; however, existing findings had a limitation in assessing the population-representative association between violence and PM2.5 due to the limited data availability: most studies have been based on homicides in monitored urban areas. This study collected violence data from the National Hospital Discharge In-depth Injury Survey in South Korea (2015-2019), based on population-representative samples. To cover unmonitored areas, we used the daily modeled PM2.5, the predicted result driven by a machine-learning ensemble model covering all inland districts in South Korea (R2>0.94). We evaluated the national association between short-term exposure to PM2.5 and violence cases with a time-stratified case-crossover design. A total of 2,867 violence cases were included. We found an approximately linear association between short-term exposure to PM2.5 (lag 0-2 days) and an increased risk of violence, with an estimated odd ratio (OR) per 10 μg/m3 of PM2.5 of 1.07 with 95% CI: 1.02-1.12. This relationship was more prominent in males and individuals aged 64 years or less than in females and individuals aged 65 years or older for the most part. The estimated excess fraction of violence cases attributable to PM2.5 was 14.53% (95% CI: 4.54%-22.92%), and 6.42% (95% CI: 1.97%-10.26%) of the excess violence was attributable to non-compliance with the WHO guidelines (daily PM2.5 > 15 μg/m3). Our findings might be evidence of the need to establish elaborate action plans and stricter air quality guidelines to reduce the hazardous impacts of PM2.5 on violence in South Korea.

Sexual dissatisfaction and its association with health status among older adults in China: a nationwide study.

Background Most population-based sexual health research in China excludes older adults. To fill the gap, this study aims to characterise sexual dissatisfaction among people aged 50years or older from a nationwide, population-representative sample and to explore its association with physical, mental, and self-reported overall health indicators. Methods Data were collected as part of the China Family Panel Studies in 2020, led by the Institute of Social Science Survey of Peking University. Multivariable logistic regressions with robust estimators were used to investigate the association between sexual dissatisfaction and health indicators and potential demographic confounders. Results Among the 8222 partnered Chinese adults aged 50years or older (median age: 59, IQR: 54-66, 47% identified as women), 78% (6380/8222) reported being satisfied or very satisfied in their sex life. After adjusting for demographic variables, poor self-rated health status (aOR: 1.59, 95% CI: 1.42-1.77), experiencing depression symptoms (aOR: 2.02, 95% CI: 1.80-2.26), and having chronic diseases (aOR: 1.20, 95% CI: 1.07-1.36) were positively associated with sexual dissatisfaction in multivariable analyses. Among sociodemographic factors, younger age, female gender, and education level at senior high school or above were more likely to experience sexual dissatisfaction (all P Conclusion Based on our sample, more than one in five Chinese adults aged 50years or older might face sexual dissatisfaction. Comorbidities common in older age likely exacerbate sexual dissatisfaction. Greater attention to sexual satisfaction research and sexual health programs among older adults is needed with respect to gender differences and chronic disease comorbidities.

Changes in food behaviours during the first COVID-19 pandemic lockdown in Australia.

The objective of this study was to explore changes in Australian consumer food behaviours during COVID-19 public health restrictions (lockdown), to provide insights into how this unforeseen crisis event affected dietary behaviour patterns. An online cross-sectional survey was conducted in September 2020 with a nationally representative sample of the Australian adult population. Participants were asked to complete questions about (1) meal planning and food purchasing and (2) barriers to cooking, before and during a national-wide COVID-19 lockdown in early 2020. A survey-adjusted logistic regression analysis was used to identify food behaviour changes resulting from the lockdown. A total of 4022 respondents completed the survey. Overall, food behaviour changes were found to be small and mostly positive. Eight of the 14 meal planning and purchasing related behaviours improved. This included more frequent meal planning and more time to be able to cook. However, not all changes were healthier, with more respondents reporting that they cooked meals at home using healthy ingredients less frequently during the lockdown. These findings demonstrate that people are willing and able to make some positive changes when they have time but that more work needs to be done to ensure that the improvements in food literacy result in healthier meals. Further consideration also needs to be given to how we can embed and amplify these positive changes into everyday habits now that public health restrictions have lifted and Australia is inpost-lockdown reality.

The relationship between lifecourse traumatic events and pain in an older rural South African population: A cross-sectional study.

Pain in older adults is an increasing concern in low- and middle-income countries (LMICs), with literature suggesting an association with past traumatic events (TEs) in high-income settings. We aim to investigate this relationship in a population-representative sample of older adults with high burden of TEs in a rural South African community. The Health and Aging in Africa: A longitudinal Study of an INDEPTH Community in South Africa (HAALSI) study collected data pain intensity, using the Brief Pain Inventory, and TEs with a 16-item questionnaire, from 2411 participants aged 40-79 in 2014-15. We used logistic regression models to test the association between TE exposure and self-reported pain status. TE experience was near-universal (99.1% experience of at least one), while 9.0% of participants reported current pain, of which 86.6% was moderate/severe. In multivariable regression, increased odds of moderate/severe pain was associated with more TEs of any kind (OR 1.08; 95%CI 1.02-1.15 per additional TE) and with past exposure to disasters, accidents and illnesses (men and women), violence in the community (women only) and social/family environment problems (men only)-but not with childhood or war-related TEs. TEs were associated with pain even within a rural resource-limited setting where trauma experiences were extremely common. However, associations varied by TE type and sex. Interventions to prevent pain in older adults need to be targeted to block specific mechanisms that vary within even at-risk populations.

Exploring the social value and design preferences for a home-based dementia community program in Australia.

We study the monetary value and the relative importance of several program characteristics for an evidence-based intervention provided at home for people living with dementia and their carers in Australia. Using a discrete choice experiment, advised through an expert and consumer co-design approach, we consider the total number of sessions, the delivery mode, the primary outcome and focus of the program as well as its costs as attributes. Results from a representative sample of the Australian adult population show a high willingness to pay for the program overall, even greater than the actual costs. Choice data from 940 respondents show preferences for in-person sessions over telehealth options and respondents place a high value on improving mood and dementia-related behaviour as well as independence in daily activities. Preference heterogeneity shows that people who have experience with home care services place an even higher monetary value on the program, compared to the rest of the sample. In light of the increased emphasis of governments on expanding home care options over residential care, these results contribute towards the design and implementation of a home-based program for people with dementia and their carers and highlight its social value.

Risk messaging style and its effect on public preparedness for earthquakes: longitudinal intervention-based study.

This study examines the effect of risk communication styles (fear-based versus empowerment-based) on households' earthquake preparedness. An online longitudinal study with intervention and control groups was conducted using a representative sample of the adult population in Israel. The change in the reported level of preparedness was assessed through Repeated Measures ANOVA with interaction effects for both the risk communication style and gender. The Analysis revealed a significant difference in reported levels of earthquake preparedness over time (F(1.697,303.70) = 102.58, p < 0.001; partial η2 = 0.36). However, no statistically significant interaction was found with the risk communication style (p = 0.55). Borderline significance (p = 0.04) was observed in the three-way interaction (time-intervention-gender). Gender (β = 0.19), age (β = 0.21), perceived earthquake likelihood (β = 0.14), and sense of preparedness (β = 0.28) were significant predictors in multivariate regression analysis. While consistently showing that participants exposed to empowering information reported higher earthquake preparedness, the research hypothesis was not substantiated. Recommendations for public health policy are discussed.

Update of French migraine epidemiology: A narrative review

This narrative review concerned the studies performed on representative samples of the French general population carried out over more than thirty years and aims to provide an update on the French migraine epidemiology. Eleven studies were selected (GRIM-1, MIG-ACCESS, GRIM-2, FRAMIG-2000, FRAMIG-3, GRIM-3, EUROLIGHT, IBMS, SNDS PACA/CORSICA, EGB FRANCE, CaMEO-I). The data extracted relates to four healings: prevalence and distribution, individual burden, recognition and care and societal burden.

High-sensitivity cardiac troponin T and N-terminal b-type natriuretic propeptide are associated with cardiac and all-cause mortality in older adults - A population-based ten-year follow-up study.

Cardiac troponin T (cTnT) and N-terminal B-type natriuretic propeptide (proBNP) are mainly used as biomarkers to diagnose specific conditions of the heart, but they also have predictive ability. Our aim was to study their associations with cardiovascular and all-cause mortality in an older population in non-acute conditions. A population-based study with a ten-year follow-up. The data comes from a community-based representative sample of an older population with 1260 participants (participation rate 82 %). Associations were analyzed using Cox proportional hazard models. Altogether, 467 (37%) subjects died during the 10-year follow-up period, and 149 of those of a cardiovascular disease. Both elevated cTnT and proBNP concentrations were statistically significantly associated with cardiovascular and all-cause mortality in older adults. Our study shows that older population with higher cTnT and proBNP concentrations have an increased risk of cardiovascular and all-cause mortality. Acknowledging the elevated risk may aid in targeting follow-up, prevention, and treatment adequately and more individually.

Explaining Political Differences in Attitudes to Vaccines in France: Partisan Cues, Disenchantment with Politics, and Political Sophistication

Context: The role of political identities in determining attitudes to vaccines has attracted a lot of attention in the last decade. Explanations have tended to focus on the influence of party representatives on their sympathizers (partisan cues). Methods: Four representative samples of the French adult population completed online questionnaires between July 2021 and May 2022 (N = 9,177). Bivariate and multivariate analyses were performed to test whether partisan differences in attitudes to vaccines are best explained by partisan cues or by parties’ differences in propensity to attract people who distrust the actors involved in vaccination policies. Findings: People who feel close to parties on the far left, parties on the far right, and green parties are more vaccine hesitant. The authors found a small effect of partisan cues and a much stronger effect of trust. More importantly, they show that the more politically sophisticated are less vaccine hesitant and that the nonpartisan are the biggest and most vaccine hesitant group. Conclusions: The literature on vaccine attitudes has focused on the case of the United States, but turning attention toward countries where disenchantment with politics is more marked helps researchers better understand the different ways trust, partisanship, and political sophistication can affect attitudes to vaccines.

Gendered Divides: Exploring How Politicians’ Gender Intersects with Vertical Affective Polarisation

Gendered Divides: Exploring How Politicians’ Gender Intersects with Vertical Affective Polarisation This article investigates the prevalence of vertical affective polarisation in the multi-party and consociational context of Flanders (Belgium) and explores how politicians’ gender intersects with vertical affective polarisation. More specifically, we test whether gender dissimilarity (voter and politician being of opposite gender) and/or gender-based stereotyping (female and male politicians taking positions on issues they are stereotypically not associated with) temper or reinforce vertical affective polarisation. Our results, based on an online survey experiment conducted among a representative sample of the general population in Flanders (Belgium), show that respondents’ level of disagreement with politicians’ policy positions significantly influences their evaluation of politicians’ general likability and psychological traits. Contrary to our expectations, however, the relationship between ideological disagreement and vertical affective polarisation is not moderated by politicians’ gender. By delving into the relationship between vertical affective polarisation, disagreement and gender, this study provides valuable insights into the interplay between identity, disagreement and affective divide.

Unveiling Awareness: Exploring Antibiotic Hypersensitivity among International Islamic University Malaysia (IIUM) Undergraduates in Kuantan, Pahang

Background: The condition known as antibiotic hypersensitivity, or antibiotic allergy, poses serious problems for the medical field. Despite its importance, little is known about the awareness of antibiotic hypersensitivity, particularly among undergraduate students in Malaysia. The purpose of this study is to assess the awareness of antibiotic hypersensitivity among IIUM Kuantan undergraduate students to improve drug allergy education and healthcare practices. Methods: A general qualitative design was used to conduct the study. Semi-structured interviews were used to gather information about the awareness of antibiotic hypersensitivity. Due to their similar prevalence of antibiotic hypersensitivity, medical students served as a representative sample of Malaysia's adult population among undergraduate students from a variety of faculties. Thematic analysis was used to analyse the data. Results: Six people in all were enlisted to take part in the study. Most of the participants were female Malay students at International Islamic University Malaysia (IIUM) Kuantan Campus, Pahang, Malaysia. Two primary themes emerged from the study: (1) Antibiotic hypersensitivity awareness and (2) Factors contributing to antibiotic hypersensitivity. This finding indicated that students' knowledge of antibiotic hypersensitivity is still lacking. Conclusion: The research found evidence to suggest that additional intervention is necessary to raise awareness within the current community. Putting skill training into practice could be a good way to raise students' awareness in this area. On the other hand, more information is needed to determine the precise kind of skill training that Malaysia should adopt.

Associations of leisure-time and occupational physical activity with allergic rhinitis and chronic sinusitis in middle-aged adults: a population-based study.

To explore the links of leisure-time physical activity (LTPA), and occupational physical activity (OPA) with allergic rhinitis (AR) and chronic rhinosinusitis (CRS) in a nationally representative sample of the Korean population. 10,346 participants aged 40-60 years old were obtained from the KNHANES 2014-2018 database. Physical activity levels were assessed using questions adapted from the Korean version of the Global Physical Activity Questionnaire. Participants were categorized as having high or low LTPA or OPA. AR and CRS statuses were determined based on participants' medical histories. Univariate and multivariate logistic regression analyses were conducted to uncover the associations between AR/CRS and LTPA/OPA. Subgroup analysis was performed considering various clinical characteristics. The multivariate regression analysis revealed high OPA as a risk factor for AR in middle-aged adults (odds ratio [OR] = 1.474, 95% confidence interval [CI]: 1.130, 1.924), especially among males (OR = 1.665, 95% CI: 1.147, 2.417), individuals with BMI ≥ 25 (OR = 1.717, 95% CI: 1.124, 2.623), blue-collar workers (OR = 1.686, 95% CI: 1.074, 2.642), and green-collar workers (OR = 4.178, 95% CI: 1.642, 11.319). However, no notable correlation was revealed between LTPA and AR prevalence (P > 0.5), nor between OPA or LTPA and CRS prevalence (P > 0.5). LTPA and OPA exhibit different links with AR, suggesting that high levels of OPA in AR individuals should be addressed. However, neither LTPA nor OPA shows a significant correlation with CRS.

Using simulations of future extreme weather events to escape the resilience trap: Experimental evidence from Hong Kong

Hong Kong is a hyper-dense coastal city that has long learned to live with a potentially disastrous extreme weather event: tropical cyclones. This was largely a reactionary process, with investments in soft and hard infrastructure made in the aftermath of devastating tropical cyclones. While the experiences of devastating tropical cyclones remain strong in the collective memory of the city, Hong Kong's present-day resilience has led to complacency, especially among the general public. We suggest that Hong Kong may be caught in a resilience trap, where previous success in building resilience may be hindering the city's ability to adapt to the impacts of future tropical cyclones. We use downward counterfactual modelling and an experimental framework to test whether simulating and visualizing the impacts of a future tropical cyclone can substitute for first-hand experience and allow individuals to experientially process the expected future impacts of tropical cyclones. Using experimental data collected from a representative sample of the general population (n = 1240), we find that simulating the impacts of a future tropical cyclone can partially substitute for first-hand experience, increase risk perceptions, and help Hong Kong escape the resilience trap.

Validation and TaqMan Conversion of a Molecular Chronotype Assessment Approach.

The present study aimed to develop a TaqMan genotyping card for molecular chronotype assessment based on a predictive panel of 35 previously identified genetic variants. A reliable TaqMan assay was successfully developed for 33 out of the 35 chronotype-predictive variants. The resulting TaqMan genotyping card was utilized to genetically characterize 196 new individuals (in addition to the previously studied 96) and the Morningness-Eveningness Questionnaire was utilized for their phenotypical chronotype assessment. The predictive panel performance was validated on (a) a group of morning and evening individuals (logistic regression model), (b) a representative sample of the original study population also including intermediate chronotypes (linear regression model) and, (c) 25,986 individuals from the Estonian Biobank, for whom Munich Chronotype Questionnaire scores were available. The validation of the morningness-eveningness logistic regression model on 25 morning and 21 evening types resulted in a predictive value of 72%, confirming the reliability of the predictive panel and the success of its conversion into a TaqMan genotyping card. By contrast, the inclusion of intermediate individuals in the model led to a significant decrease in predictive performance (45% on 100 individuals [25 morning, 54 intermediate, and 21 evening]), with intermediate types being the most affected. No significant associations were observed between the genotype panel and chronotype in the Estonian Biobank sample. In conclusion, our genotyping card might represent a promising molecular chronotyping tool for the Italian population. Its performance in other populations is worthy of further study.