In this study, we used next-generation sequencing to obtain the complete mitochondrial genome of Jaydia lineata. This mitochondrial genome, consisting of 16,510 base pairs (bp), contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and 2 noncoding control regions (control region and origin of light-strand replication) as those found in other vertebrates. Control region, of 848 bp in length, is located between tRNAPro and tRNAPhe. Within the control region, typical conserved domains, such as the termination-associated sequence, central and conserved sequence blocks domains were identified. The overall base composition shows 26.71% of T, 28.46% of C, 27.24% of A and 17.58% of G, with a slight A + T rich feature (53.95%). The complete mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics.