About thirty seven million people worldwide were living with human immunodeficiency virus in the year 2017, of these, 1.8 million were children (<15 years old). Most of these children live in sub-Saharan Africa and were infected by their human immunodeficiency virus from positive mothers during pregnancy, childbirth or breastfeeding. The study aimed to assess human immune deficiency virus exposed infants feeding practices and associated factors among mothers on prevention of mother to child transmission clinics at Ambo town public health institution. Facility based cross-sectional study was conducted from May to August 2019, at Ambo town public health institution. Systematic random sampling method was utilized to select study respondents. Data were collected through an exit interview by using pre-tested structured questionnaire. The returned questionnaires were checked for completeness, cleaned manually and entered to epidata version 3.1 and then exported SPSS version 20.0 for further analysis. Univariate and multivariate logistic regression analyses were carried out and finally descriptive finding was presented using frequency distribution tables and graphs. From total of 106 respondents 44 (42%) were greater than or equal to 30 years old and majority (75.5%) were from urban and more than half (56.8%) had good knowledge towards infant feeding practice. About 53.8% of the children were exclusively breast fed, whereas 28.3%) and 17.9%had practiced mixed feeding and exclusive replacement feeding respectively. This study result identified that the main reason reported for Exclusive Breast Feeding were advised by health professionals, easily availability of breast milk and nutritional importance among the study population. Thus Health professionals working at prevention of mother to child transmission clinic should work on infant feeding practice options by using mass media, community mobilization and health education in more comprehensive.