A web-based program was developed to conduct brief multiple-stimulus without replacement preference assessments for videos (e.g., movies, cartoons, music videos). The preference assessment program was used with two populations: young adults with developmental disabilities and school-age children with emotional and behavioral needs. Stimulus preference hierarchies were identified for all participants, indicating that a web-based preference assessment procedure is an efficient procedure for isolating highly preferred videos, which might be useful as reinforcers in a variety of settings.