Background: Increasing the penetration of renewable energy sources has become necessary, especially in isolated microgrids. This increase leads to a decrease in the total inertia of the microgrids, which affects microgrid stability. Moreover, voltage and frequency control in lowinertia microgrids is more difficult and sensitive. Objective: Improve low inertia isolated microgrids' dynamic response and save the microgrid stability at different contingency and uncertainty conditions. Moreover, it allows for more penetration of renewable energy sources. Method: Proposing different control strategies based on virtual inertia control. The first is a proportional- integral-derivative (PID) controller, and then, to allow for more tuning flexibility, a fractional- order proportional-integral-derivative (FOPID) controller is used. MATLAB TM/Simulink is used to compare the response of the isolated microgrid without virtual inertia control, with conventional virtual inertia control, PID-based virtual inertia control, and FOPID-based virtual inertia control. The PID and FOPID controllers’ parameters are tuned using the ant colony optimization (ACO) technique. Results: The proposed control techniques save the isolated microgrids' stability at different penetration levels of renewable energy sources and operating conditions. At the same time, the isolated microgrid without virtual inertia control or conventional virtual inertia control can not save its stability in many operating conditions. Conclusion: The proposed fractional-order proportional-integral-derivative (FOPID)-based virtual inertia control has proven its effectiveness in saving the isolated microgrid stability and gives the best controller response. FOPID-based virtual inertia control minimizes the frequency deviation with different disturbances and operating conditions.