Luminex bead-based assays has been widely used for detection of anti-human leukocyte antigen (HLA) donor-specific antibodies (DSA) which are considered as a risk factor for acute rejection. Luminex single antigen assays might be more promising technique for determination of DSA. The aim of this study was to compare these techniques used to detect DSA in our Renal Transplant Candidates. Sixteen sera from 16 renal transplant candidates who had positive result with Luminex bead-based assays were screened for the presence of DSA by the Luminex single antigen assays. All sera from 16 sensitized candidates were also positive for Luminex single antigen assay. The results were as follows: Seven sera HLA-A ∗ 2301(43,75%), five sera HLA-A 2402 (31,25%), five sera HLA-A 2403 (31,25%); six sera HLA-B ∗ 8202 (37,5%); six sera HLA-C ∗ 0501 (37,5%), five sera HLA-C 0801 (31,25%); seven sera HLA-DQA ∗ 0201 (43,75%); six sera HLA-DQB1 ∗ 0401 (37.5%), six sera HLA-DQB1 ∗ 0202 (37.5%), four sera HLA-DQB1 ∗ 0402 (%25); four sera HLA-DRB1 ∗ 0901 (25%), four sera HLA-DRB1 ∗ 0701 (25%); and three sera HLA-DPB1 ∗ 1701 (18.75%) were found to be positive. HLA-A ∗ 2301 and HLA-DQA ∗ 0201 positivity were found more frequent in Turkish renal transplant candidates. Detection of pre-transplant DSA by using Luminex single antigen assay would be beneficial for identifying high-risk candidates.