To evaluate the use of magnetic resonance fingerprinting (MRF) for simultaneous quantification of and in a single breath-hold in the kidneys. The proposed kidney MRF sequence was based on MRF echo-planar imaging. Thirty-five measurements per slice and overall 4 slices were measured in 15.4seconds. Group matching was performed for in-line quantification of and . Images were acquired in a phantom and 8 healthy volunteers in coronal orientation. To evaluate our approach, region of interests were drawn in the kidneys to calculate mean values and standard deviations of the and times. Precision was calculated across multiple repeated MRF scans. Gaussian filtering is applied on baseline images to improve SNR and match stability. and times acquired with MRF in the phantom showed good agreement with reference measurements and conventional mapping methods with deviations of less than 5% for and less than 10% for . Baseline images in vivo were free of artifacts and relaxation times yielded good agreement with conventional methods and literature (deviation , ). In this feasibility study, the proposed renal MRF sequence resulted in accurate and quantification in a single breath-hold.