Renal Slices Research Articles

Metabolism of renal cortex in nephrotic syndrome of rats.

Summary1. The oxygen uptake of renal cortex slices of 29 control rats and 42 animals in which the nephrotic syndrome had been induced by the intravenous injection of nephrotoxic sera was studied. 2. In Krebs-Ringer solution, without added substrates, the average oxygen uptake of cortex slices of nephrotic rats did not differ from that of the controls. 3. Addition of glucose or of fructose resulted invariably in an increase in the oxygen uptake in the control slices. No consistent increase after addition of these two hexoses was noted when the kidney slices were obtained from nephrotic rats. 4. The increase in oxygen uptake of nephrotic slices produced by lactate, succinate, glutamate, acetoacetate and butyrate was equal to or only slightly less than in the corresponding control experiments. 5. Racemic alanine stimulated the oxygen uptake of the slices to a lesser degree in the nephrotic group. 6. Since oxidation of lactate, of succinate, and of glutamate proceeded at a normal rate, the inability of glucos...

A quantitative method for study of phenolsulfonphthalein (PSP) concentration by surviving renal slices.

SummaryA method has been described for the quantitative extraction and spectro-photometric estimation of phenosulfon-phthalein (PSP) in renal cortex slices of the guinea pig. The effect of alterations of time of incubation, concentration of the PSP in the medium, and weight of tissue on the in vitro uptake of PSP by this preparation have been investigated. Uptake of PSP increases progressively with increases in incubation time. Increasing the concentration of PSP in the medium results in a decrease in the ratio of dye concentration in the slice to that in the medium. Ability of the slice to concentrate PSP from the medium is not markedly influenced by alterations of tissue weight between 200 and 400 mg.