Background: Viral Hepatitis is a global disease, affecting millions of patients around the world. Dialysis dependent patients used as an artificial kidney (hemodialyzer) to remove waste product from the blood in severe renal impairment patients. Hence they are more vulnerable to viral hepatitis. Aims:The aim of this study is to investigate the prevalence of hepatitis B and C infections among dialysis patients in the Qassim province, Saudi Arabia. We further examine their epidemiological profile, risk factors, prognostic factors, treatment course, and characterization of their liver disease stage. Methods: This study is a cross-sectional study. Hepatitis B and C positive patients receiving dialysis in Qassim Province were included in this study. We reviewed the data registry of dialysis centers where medical records of hepatitis B surface antigen (HBsAg) and hepatitis C virus (HCV) serology are reported from 18 affiliated dialysis centers across Al Qassim Region. The prevalence was calculated by dividing the number of patients with positive HBsAg or HCV serology to the total number of patients. Results: The prevalence of Hepatitis B Surfaces Antigen (HBsAg) positive cases among in-center hemodialysis patients was 20 (03.2%) with 12 (01.9%) of cases converting from HBsAg negative to positive during the previous 12 months. While the prevalence of Hepatitis C antibody positive cases was 40 (06.4%) with 2 cases converting from negative to positive during the previous 12 months. Conclusion: This study finds low intermediate prevalence HBV and high prevalence for HCV according to CDC classification. Funding Statement: The authors received no financial support for the research, authorship, and/or publication of this article. Declaration of Interests: The authors declare that they have no conflicts of interest. Ethics Approval Statement: This paper is approved by Qassim Research Ethics Committee.