During the Renaissance, the revival of ancient Roman culture was of great practical significance. The Renaissance was a cultural movement that emphasized humanism, the revival of classical culture, and artistic innovation, seeking to transcend the Dark Ages of the Middle Ages and restore the glory of ancient culture and art. By re-learning and borrowing from ancient Roman culture, Renaissance artists attempted to re-explore the natural, religious, political, and social issues of mankind through the aesthetic expression and social function of art. This paper analyses the practical significance of reviving ancient Roman culture through the study of the background of the Renaissance era. Taking Leonardo da Vinci's work "Mona Lisa" as an example, starting from the painting technique of "Mona Lisa", explores the artist's understanding and expression of art, as well as the meaning behind the artwork. The purpose of this paper is to explore the aesthetic expression as well as the social function of "art" during the Renaissance, using "art" as a clue. And finally, conclude that Renaissance art had a profound influence on the development of later generations and became an important milestone in the history of Western art.