Alterations of the cornified layer imply barrier defects, including the transepidermal water loss (TEWL), dehydration, desquamation and loss of lipid contents, which are all found in several inflammatory skin diseases, such as Atopic Dermatitis (AD). AD is characterized, among other factors, by altered epidermal differentiation and activation of the immune system at the skin level. Emollients are a standard of care, steroid-sparing, and useful for both prevention and maintenance therapy in AD. AD-DER is an emollient formulated specifically for skin affected by AD, which contains several active agents (liquorice extract, niacinamide, xylitol, laureth-9-polydocanol) to target the underlying AD disease pathophysiology. The aim of this study was to evaluate the efficacy of AD-DER emollient in the in vitro tape stripping (TS) AD model. TS showed a mechanically-induced skin barrier disruption, by 100 repeated applications and subsequent removal of type 3M adhesive tapes. 3D cultures were maintained in air-liquid interface in order to preserve the original tissue architecture and to obtain a vertical growth regenerating epidermis. TS model presented a complete removal of stratum corneum and lipids, a drastic decrease of structural and adhesion proteins, that are normally decreased in AD. AD-DER treatment in vitro restored the lipid content and the expression of the tight junction marker claudin, and the levels of differentiation markers, such as involucrin and caspase-14. This indicates that AD-DER tends to reinstate a normal differentiation, contributing to the normalization of epidermal homeostasis. In addition, the increase of TLR-1 and TLR-2 expression together with the decrease of keratin 16 (K16) levels after AD-DER treatment could be beneficial, given the TLR-1/TLR-2 reduced expression and K16 increased expression in AD.
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