Removal Of Precursors Research Articles

Removal of disinfection by-product precursors by coagulation and an innovative suspended ion exchange process

This investigation aimed to compare the disinfection by-product formation potentials (DBPFPs) of three UK surface waters (1 upland reservoir and 2 lowland rivers) with differing characteristics treated by (a) a full scale conventional process and (b) pilot scale processes using a novel suspended ion exchange (SIX) process and inline coagulation (ILCA) followed by ceramic membrane filtration (CMF). Liquid chromatography-organic carbon detection analysis highlighted clear differences between the organic fractions removed by coagulation and suspended ion exchange. Pretreatments which combined SIX and coagulation resulted in significant reductions in dissolved organic carbon (DOC), UV absorbance (UVA), trihalomethane and haloacetic acid formation potential (THMFP, HAAFP), in comparison with the SIX or coagulation process alone. Further experiments showed that in addition to greater overall DOC removal, the processes also reduced the concentration of brominated DBPs and selectively removed organic compounds with high DBPFP. The SIX/ILCA/CMF process resulted in additional removals of DOC, UVA, THMFP, HAAFP and brominated DBPs of 50, 62, 62, 62% and 47% respectively compared with conventional treatment.

Nitrosamine Precursor Removal by BAC: A Case Study of Adsorption Versus Biotreatment

Biological activated carbon (BAC) can effectively remove nitrosamine (NA) precursors. However, the relative significance of adsorption and biotreatment in the NA removal process remains unclear. In this study, organic matter in the influent of a pilot‐scale BAC plant was separated into four fractions that were adsorbable, biodegradable, or both. Bench‐scale tests showed that 70% of the N‐nitrosodimethylamine formation potential was biodegradable and 42% was adsorbable. In addition, 45% of the N‐nitro sodiethylamine formation potential was biodegradable and 59% was adsorbable. Biological treatment included two consecutive actions: biosorption, which was quick and accounted for 10% of the removal of NA formation potential, and biodegradation, which accounted for 70% of the removal of NA formation potential but took up to seven days to maximize. Activated carbon adsorption during bench‐scale tests was capable of effectively removing NA formation potential in 7 h, but its performance was impaired in pilot‐scale tests because of declining adsorption capacity after extended operation and shorter contact time in the BAC filter.

Improved (and Singular) Disinfectant Protocol for Indirectly Assessing Organic Precursor Concentrations of Trihalomethanes and Dihaloacetonitriles.

Measurements of disinfection byproduct (DBP) organic precursor concentrations (OPCs) are crucial to assess and improve DBP control processes. Typically, formation potential tests - specified in Standard Methods (SM) 5710-B/D - are used to measure OPCs. Here, we highlight several limitations of this protocol for dihaloacetonitriles and trihalomethanes and validate a novel Alternative Method (AM). The effects of pH, disinfectant type (free chlorine and monochloramine), and chlor(am)ine residual (CR) were examined on DBP formation in a suite of waters. Using the SM, DHAN decreased 43-47% as the CR increased from 3 to 5 mg L(-1) as Cl2, compromising OPC assessments. In contrast, a high monochloramine dose (250 mg L(-1) as Cl2) at pH 7.0 (the AM) accurately reflected OPCs. The two methods were compared for assessing DBP precursor removal through three granular activated carbon (GAC) columns in series. Breakthrough profiles assessed using the AM only showed DBP precursor sorption occurred in each column that decreased over time (p = 0.0001). Similarly, the AM facilitated ranking of three types of GAC compared in parallel columns, whereas the SM produced ambiguous results. Fluorescence intensity of a humic-like fluorophore (i.e., I345/425) correlated strongly to precursor removal in the GAC columns. The practical implications of the results are discussed.

Comparisons between biological filtration and coagulation processes for the removal of dissolved organic nitrogen and disinfection by-products precursors

Effects of biological aerated filter (BAF), sand filter (SF), biological activated carbon (BAC), alum coagulation, and Moringa oleifera coagulation on the removal of DOC and DON from reservoir water were investigated. The DOC removal efficiency was in the following sequence: BAC (56%), BAF (51%), SF (45%), alum (27%), and M. oleifera (22%). The trend for DON removal efficiency was similar to that of DOC. The better performance by BAC was attributed to the effective microbial breakdown of organic matter and the adsorption of some molecules onto activated carbon. The organic content with a molecular weight (MW) of less than 10 kDa accounted for more than 45% of the total DOC, whereas a MW of less than 1 kDa accounted for 50% of the total DON. The biofilters removed the entire MW range of organic contents, unlike the coagulants which only removed organics with a MW of more than 10 kDa. Consistent with greater DOC and DON removal, BAC led to greater removal of DBP precursors as shown by DBP formation potentials.

The enhanced removal of carbonaceous and nitrogenous disinfection by-product precursors using integrated permanganate oxidation and powdered activated carbon adsorption pretreatment

Pilot-scale tests were performed to reduce the formation of a range of carbonaceous and nitrogenous disinfection by-products (C-, N-DBPs), by removing or transforming their precursors, with an integrated permanganate oxidation and powdered activated carbon adsorption (PM–PAC) treatment process before conventional water treatment processes (coagulation–sedimentation–filtration, abbreviated as CPs). Compared with the CPs, PM–PAC significantly enhanced the removal of DOC, DON, NH3+–N, and algae from 52.9%, 31.6%, 71.3%, and 83.6% to 69.5%, 61.3%, 92.5%, and 97.5%, respectively. PM pre-oxidation alone and PAC pre-adsorption alone did not substantially reduce the formation of dichloroacetonitrile, trichloroacetonitrile, N-nitrosodimethylamine and dichloroacetamide. However, the PM–PAC integrated process significantly reduced the formation of both C-DBPs and N-DBPs by 60–90% for six C-DBPs and 64–93% for six N-DBPs, because PM oxidation chemically altered the molecular structures of nitrogenous organic compounds and increased the adsorption capacity of the DBP precursors, thus highlighting a synergistic effect of PM and PAC. PM–PAC integrated process is a promising drinking water technology for the reduction of a broad spectrum of C-DBPs and N-DBPs.

Simple preparation of new [(18) F]F-labeled synthetic amino acid derivatives with two click reactions in one-pot and SPE purification.

New [(18) F]fluorinated 1,2,3-triazolyl amino acid derivatives were efficiently prepared from Huisgen 1,3-dipolar cycloaddition reactions, well known as click reaction. We developed two simultaneous click reactions in one-pot with a simple solid-phase extraction (SPE) purification method. [(18) F]fluoro-1-propyne was obtained at a 45% non-decay corrected radiochemical yield based on the [(18) F]fluoride ion. The one-pot and simultaneous two click reactions were performed with unprotected azido-alkyl amino acid, [(18) F]fluoro-1-propyne, and lipophilic additive alkyne to produce three synthetic amino acid derivatives, AMC-101 ([(18) F]-6a), AMC-102 ([(18) F]-6b), and AMC-103 ([(18) F]-6c) with 29%, 28%, and 24% of non-decay corrected radiochemical yields, respectively. All radiotracers indicated that radiochemical purities were >95% without any residual organic solvent. Our new method involving two click reactions in one-pot showed high radiochemical and chemical purity by easy removal of the residual precursor from the simultaneous two click reactions.

Engineered biofiltration for the removal of disinfection by-product precursors and genotoxicity

Disinfection by-products (DBPs) are formed when naturally occurring organic matter reacts with chlorine used in drinking water treatment, and DBPs formed in chlorinated drinking water samples have been shown to cause a genotoxic response. The objective of the current study was to further understand the principles of biofiltration and the resulting impacts on the formation of DBPs and genotoxicity. Pilot-scale systems were utilized to assess the performance of engineered biofilters enhanced with hydrogen peroxide, in-line coagulants, and nutrients when compared to passively operated biofilters and conventional treatment (coagulation, flocculation, sedimentation, non-biological filtration). Organic fractionation was completed using liquid chromatography-organic carbon detection (LC-OCD). Water samples were chlorinated after collection and examined for the removal of trihalomethane (THM), haloacetic acid (HAA), and adsorbable organic halide (AOX) precursors. Additionally, the formation potential of two halogenated furanones, 3-chloro-4(dichloromethyl)-2(5H)-furanone (MX) and mucochloric acid (MCA), and genotoxicity was determined. Biofiltration was shown to preferentially remove more DBP precursors than dissolved organic carbon (DOC). Formation potential of the unregulated DBPs, including MX and MCA, and genotoxic response was shown to be correlated to THM formation. These results infer that monitoring for THMs and HAAs provide insight to the formation of more mutagenic DBPs such as halogenated furanones, and that biofiltration may preferentially remove precursors to DBPs at a rate exceeding the removal of DOC.

An evaluation of organic substance fraction removal during ion exchange with Miex-DOC resin.

In this study, the usefulness of Miex-DOC resin in eliminating organic substances and their fractions from water sources for drinking water was evaluated. The objects of study were samples from three surface water sources and one infiltration water source taken at water treatment plants before treatment in technical conditions. In particular, the effectiveness of removing biodegradable and non-biodegradable fractions as a function of resin dosages and water-resin contact times was evaluated. The ion exchange process with the Miex-DOC resin achieved a high effectiveness in removing aromatic non-biodegradable organic substances, and therefore a reduction in UV254 absorbance. The biodegradable fraction is much less susceptible to removal yet its removal effectiveness allows for a significant reduction in hazards connected with secondary microorganism development. The results of this study indicate the possibility of using ion exchange with the Miex-DOC resin for effective removal of disinfection by-product precursors.

Photocatalysis of THM precursors in reclaimed water: the application of TiO2 in UV irradiation

In this study, ultraviolet (UV) irradiation followed by chlorination was employed for reclaimed water disinfection. In order to reduce trihalomethanes (THMs) from reclaimed water, suspended TiO2 (10 mg/L) was added as photocatalyst in UV process to enhance the removal of THM precursors. Reduction of UV absorbance in 254 nm (UV254), dissolved organic carbon (DOC), and THMs formation was analyzed under different experimental conditions (exposure time, pH, TiO2 doses, and TiO2 forms). Excitation–emission matrix spectra technology was also used to investigate the changes of dissolved organic matters properties during UV and UV-TiO2 process. Expansion of irradiation time resulted in a remarkable decrease in UV254 and THM yields, but showed few influence on DOC removal. THMs yield decreased more than 50% with pH increased from 5 to 9 and rise in TiO2 dosage also presented a positive effect on photocatalytic disinfection. In addition, a dramatic increase in removal rates of UV254, DOC, and THMs was observed when TiO2 doses were increased from 3 to 15 mg/L. In terms of TiO2 form, suspended TiO2 exhibited a better removal capacity on UV254, DOC, and THMs by contrast with TiO2 coated on granular active carbon.

Disinfection byproducts in drinking water and regulatory compliance: A critical review

Disinfection by-products (DBPs) are regulated in drinking water in a number of countries. This critical review focuses on the issues associated with DBP regulatory compliance, including methods for DBP analysis, occurrence levels, the regulation comparison among various countries, DBP compliance strategies, and emerging DBPs. The regulation comparison between China and the United States (US) indicated that the DBP regulations in China are more stringent based on the number of regulated compounds and maximum levels. The comparison assessment using the Information Collection Rule (ICR) database indicated that the compliance rate of 500 large US water plants under the China regulations is much lower than that under the US regulations (e.g. 62.2% versus 89.6% for total trihalomethanes). Precursor removal and alternative disinfectants are common practices for DBP regulatory compliance. DBP removal after formation, including air stripping for trihalomethane removal and biodegradation for haloacetic acid removal, have gained more acceptance in DBP control. Formation of emerging DBPs, including iodinated DBPs and nitrogenous DBPs, is one of unintended consequences of precursor removal and alternative disinfection. At much lower levels than carbonaceous DBPs, however, emerging DBPs have posed higher health risks.

Removal of organic matter and disinfection by-products precursors in a hybrid process combining ozonation with ceramic membrane ultrafiltration

The performance of an integrated process including coagulation, ozonation, ceramic ultrafiltration (UF) and biologic activated carbon (BAC) filtration was investigated for the removal of organic matter and disinfection by-products (DBPs) precursors from micro-polluted surface water. A pilot scale plant with the capacity of 120 m3 per day was set up and operated for the treatment of drinking water. Ceramic membranes were used with the filtration area of 50 m2 and a pore size of 60 nm. Dissolved organic matter was divided into five fractions including hydrophobic acid (HoA), base (HoB) and neutral (HoN), weakly hydrophobic acid (WHoA) and hydrophilic matter (HiM) by DAX-8 and XAD-4 resins. The experiment results showed that the removal of organic matter was significantly improved with ozonation in advance. In sum, the integrated process removed 73% of dissolved organic carbon (DOC), 87% of UV254, 77% of trihalomethane (THMs) precursors, 76% of haloacetic acid (HAAs) precursors, 83%of trichloracetic aldehyde (CH) precursor, 77% of dichloroacetonitrile (DCAN) precursor, 51% of trichloroacetonitrile (TCAN) precursor, 96% of 1,1,1-trichloroacetone (TCP) precursor and 63% of trichloronitromethane (TCNM) precursor. Hydrophobic organic matter was converted into hydrophilic organic matter during ozonation/UF, while the organic matter with molecular weight of 1000–3000 Da was remarkably decreased and converted into lower molecular weight organic matter ranged from 200–500 Da. DOC had a close linear relationship with the formation potential of DBPs.

NOM removal: evaluating five process alternatives to alum coagulation

Bench-scale experiments were conducted to evaluate disinfection by-product (DBP) precursor removal by coagulation with alum and ferric sulfate (FS), intermediate ozonation (IO 3 ), powdered-activated carbon (PAC), ion exchange resin (IX), and spiral-wound nanofiltration (NF). The effect of source water quality on process performance was also assessed on six source waters. Overall, NF offered the best performance in terms of dissolved organic carbon (DOC) removals along with lower DBP concentrations in every tested condition except for DOC-rich waters (13–15 mg/L). Conventional treatment coupled with recirculated PAC adsorption (Actiflo ® Carb) was the process configuration providing the most consistently low DOC (less than 2 mg/L), facilitating DBP regulation compliance. IX/alum and alum/IO 3 reduced DBP concentration to a lesser extent, but proved to be more efficient than conventional treatment with alum alone. In terms of effluent DBP concentration, FS outperformed alum at higher coagulant dosages, whereas alum offered a superior performance at lower dosages. Finally, this investigation shows the relevance of conducting treatability assays to identify the most suitable treatment alternative for DBP control.

Effects of chlorination operating conditions on trihalomethane formation potential in polyaluminum chloride-polymer coagulated effluent

In this study, coagulation performance of polyaluminum chloride (PAC) and PAC-lignin acrylamide (PAC+LAM) in reservoir water treatment was contrastively analyzed. Effects of operating conditions including chlorine dose, contact time and pH on the formation potential of trihalomethanes (THMs) during chlorination in coagulated effluent were also investigated. Comparing with PAC, PAC+LAM achieved higher efficiency in the removal of THMs precursors. TTHM yield in unfiltered water samples (UW) was greater than that of filtered water (FW) due to the residual dissolved organic matter (DOM) in the suspended particles or micro flocs. Meanwhile, operating conditions during chlorination had a significant influence on THMs formation potential. With chlorine dose rising, mass ratio of CHCl3 to TTHM increased, whereas that of CHBr2Cl decreased due to higher Cl2/Br− molar ratio. TTHM and CHCl3 levels rose with the increase of pH. Under a given chlorination condition, there was a minor effect of contact time on THM speciation.

Effects of coagulation on the removal of natural organic matter, genotoxicity, and precursors to halogenated furanones

Natural organic matter (NOM) in drinking water can react with disinfectants to form disinfection by-products (DBPs). Halogenated furanones are a group of emerging DBPs that can account for 20–60% of the total mutagenicity observed in drinking water. This study examined the impacts of bench-scale coagulation and subsequent chlorination on DBP formation as well as genotoxicity using three source waters located in Ontario, Canada. Two halogenated furanones 3-chloro-4-(dichloromethyl)-2(5H)-furanone (MX) and mucochloric acid (MCA) were analyzed; along with trihalomethanes (THMs), haloacetic acids (HAAs), and absorbable organic halides (AOX). NOM was quantified using liquid chromatography–organic carbon detection (LC–OCD). Measured MX and MCA formation was 6.9–15.3 ng/L and 43.2–315 ng/L following optimized coagulation and subsequent chlorination of the three waters tested. DBP formation and speciation were evaluated as a function of the specific NOM fractions present in the source waters. Humics, building blocks, and biopolymers were highly correlated with DBP formation. Correlations between DBPs were also investigated and a potential relationship between MCA and/or MX vs. HAAs was observed. MX was the only measured DBP that contributed to genotoxicity, representing less than 0.001% of AOX by mass but responsible for 40–67% of the genotoxic response in chlorinated Ottawa River water samples. Genotoxic potential decreased with alum dosages, signifying that coagulation was effective at removing genotoxic DBP precursors.

Colorectal serrated pathway cancers and precursors.

The serrated pathway (SP) can be viewed as two parallel, but partially overlapping, arrays of colorectal precursor lesions, and their respective endpoint carcinomas, that are distinct from those of the conventional adenoma-carcinoma sequence (APC-pathway). In this review we focus at the outset on the clinical impact, pathological features, molecular genetics and biological behaviours of the various SP cancers. Then we summarize the clinicopathological features, classification and molecular profiles of the two main precursor lesions that anchor the respective pathways: (i) sessile serrated adenoma/polyp (SSA/P), also called sessile serrated lesion (SSL), and (ii) traditional serrated adenoma (TSA). Activating mutations of the RAS-RAF-MAPK pathway initiate and sustain the lesions of the SP, and CpG island methylation of the promoter regions of tumour suppressor and DNA repair genes play the major role in their neoplastic progression. The SP includes microsatellite stable (MSS) carcinomas that are among the most biologically aggressive colorectal carcinomas (CRC), and also accounts for the great preponderance of sporadic hypermutated, mismatch repair (MMR)-deficient or microsatellite instable (MSI) CRC. The identification, removal and appropriate classification of at-risk SP precursors and surveillance of individuals who harbour these lesions present a challenge and opportunity for CRC prevention and mortality reduction.

A wet electrostatic precipitator (WESP) as countermeasure to mist formation in amine based carbon capture

This study is to our knowledge the first to evaluate the potential of a wet electrostatic precipitator (WESP) to prevent aerosol formation issues inside amine based carbon capture installations. A WESP is a suitable option since this study proves that it is very efficient for the removal of the mist precursors inside the flue gas to be treated. Although a significant capital investment cost may be involved, energy requirements (i.e. low pressure drop), maintenance and therefore operational costs are expected to be very low. However, it is shown here that the WESP must be installed at the right location, i.e. the flue gas to be treated must contain no or very low levels of SO2. The reason is that the WESP's aerosol removal efficiency decreases strongly in the presence of SO2 gas and in a certain range also with increasing voltages. This limits the positive effect that the WESP has on reducing the MEA emissions from the absorber since a large number of mist formation precursors remain in the flue gas. In the presence of SO2, a WESP can actually produce H2SO4 aerosols. It is shown that these newly created aerosols are very small (low nanometre range). This information is very important for future pilot and demo amine carbon capture installations thinking of implementing a WESP as countermeasure to aerosol formation issues. It implies that no or very low levels of SO2 should still be present in the flue gas before entering the WESP. Since most of the amine carbon capture installations have a pre-scrubber (usually using NaOH to remove residual SO2 in the flue leaving the power plant's FGD) in front of their amine absorber, the WESP must be installed behind this pre-scrubber and not in front of it.

When do changes in cancer survival mean progress? The insight from population incidence and mortality.

It is often assumed that increases in cancer survival reflect true progress against cancer. This is true when these increases are accompanied by decreased burden of disease: Fewer people being diagnosed or dying from cancer (ie, decreased incidence and mortality). But increased survival can also occur even when incidence is increasing and mortality is unchanged. To use trends in cancer burden-incidence and mortality-to illustrate when changes in survival reflect true progress. Using data from 1975 to 2010 collected by the Surveillance, Epidemiology, and End Results Program (incidence, survival) and the National Center for Health Statistics (mortality), we analyzed US trends in five-year relative survival, age-adjusted incidence, and mortality for selected cancers to identify patterns that do and do not reflect progress. Among the nine common cancers examined, survival increased in seven, and changed little or not at all for two. In some cases, increased survival was accompanied by decreased burden of disease, reflecting true progress. For example, from 1975 to 2010, five-year survival for colon cancer patients improved (from 48% to 68%) while cancer burden fell: Fewer cases (incidence decreased from 60 to 41 per 100,000) and fewer deaths (mortality decreased from 28 to 16 per 100,000), a pattern explained by both increased early detection (with removal of cancer precursors) and more effective treatment. In other cases, however, increased survival did not reflect true progress. In melanoma, kidney, and thyroid cancer, five-year survival increased but incidence increased with no change in mortality. This pattern suggests overdiagnosis from increased early detection, an increase in cancer burden. Changes in survival must be interpreted in the context of incidence and mortality. Increased survival only represents progress when accompanied by a reduction in incidence, mortality, or ideally both.

Enhanced coagulation with powdered activated carbon or MIEX® secondary treatment: A comparison of disinfection by-product formation and precursor removal

The removal of both organic and inorganic disinfection by-product (DBP) precursors prior to disinfection is important in mitigating DBP formation, with halide removal being particularly important in salinity-impacted water sources. A matrix of waters of variable alkalinity, halide concentration and dissolved organic carbon (DOC) concentration were treated with enhanced coagulation (EC) followed by anion exchange (MIEX® resin) or powdered activated carbon (PAC) and the subsequent disinfection by-product formation potentials (DBP-FPs) assessed and compared to DBP-FPs for untreated samples. Halide and DOC removal were also monitored for both treatment processes. Bromide and iodide adsorption by MIEX® treatment ranged from 0 to 53% and 4–78%, respectively. As expected, EC and PAC treatments did not remove halides. DOC removal by EC/PAC was 70 ± 10%, while EC/MIEX® enabled a DOC removal of 66 ± 12%. Despite the halide removals achieved by MIEX®, increases in brominated disinfection by-product (Br-DBP) formation were observed relative to untreated samples, when favourable Br:DOC ratios were created by the treatment. However, the increases in formation were less than what was observed for the EC/PAC treated waters, which caused large increases in Br-DBP formation when high Br-DBP-forming water quality conditions occurred. The formation potential of fully chlorinated DBPs decreased after treatment in all cases.

Degradation of typical N-nitrosodimethylamine (NDMA) precursors and its formation potential in anoxic-aerobic (AO) activated sludge system

N-nitrosodimethylamine (NDMA) is an emerging disinfection byproduct. Removal of its potential precursors is considered as an effective method to control NDMA. In this study, four typical NDMA precursors (dimethylamine (DMA), trimethylamine (TMA), dimethylformamide (DMFA) and dimethylaminobenzene (DMAB)) were selected, and their removal capacities by activated sludge were investigated. Batch experiments indicated that removal of NDMA precursors was better under aerobic condition than anoxic condition; and their specific degradation rates follow the order of DMA > TMA > DMFA > DMAB. In anoxic-aerobic (AO) activated sludge system, the optimal hydraulic retention time and sludge retention time were 10 h and 20 d, respectively, for the removal of both NDMA precursors (four selected NDMA precursors and NDMA formation potential (NDMA FP)) and nutrients. Our results also suggested that there was a positive correlation between NDMA FP and dissolved organic nitrogen (DON) in wastewater. The removal efficiency of NDMA FP was in the range of 46.8–72.5% in the four surveyed wastewater treatment plants except the one which adopted chemically enhanced primary process. The results revealed that the AO system had the advantage of removing NDMA FP. Our results are helpful for the knowledge of the removals of NDMA precursors during activated sludge treatment processes.

Spleen supports a pool of innate-like B cells in white adipose tissue that protects against obesity-associated insulin resistance.

Lipid accumulation in obesity triggers a low-grade inflammation that results from an imbalance between pro- and anti-inflammatory components of the immune system and acts as the major underlying mechanism for the development of obesity-associated diseases, notably insulin resistance and type 2 diabetes. Innate-like B cells are a subgroup of B cells that respond to innate signals and modulate inflammatory responses through production of immunomodulatory mediators such as the anti-inflammatory cytokine IL-10. In this study, we examined innate-like B cells in visceral white adipose tissue (VAT) and the relationship of these cells with their counterparts in the peritoneal cavity and spleen during diet-induced obesity (DIO) in mice. We show that a considerable number of innate-like B cells bearing a surface phenotype distinct from the recently identified "adipose natural regulatory B cells" populate VAT of lean animals, and that spleen represents a source for the recruitment of these cells in VAT during DIO. However, demand for these cells in the expanding VAT outpaces their recruitment during DIO, and the obese environment in VAT further impairs their function. We further show that removal of splenic precursors of innate-like B cells through splenectomy exacerbates, whereas supplementation of these cells via adoptive transfer ameliorates, DIO-associated insulin resistance. Additional adoptive transfer experiments pointed toward a dominant role of IL-10 in mediating the protective effects of innate-like B cells against DIO-induced insulin resistance. These findings identify spleen-supplied innate-like B cells in VAT as previously unrecognized players and therapeutic targets for obesity-associated diseases.