1. Pampulha Lake has undergone rapid eutrophication. Annual maxima of total phosphorus and conductivity increased steadily from 1993 to 1996. Nitrogen quickly decreased after macrophyte removal in 1994, but increased again in the following years2. The zooplankton was dominated by Daphnia gessneri, D. laevis, Diaphanosoma birgei and Thermocyclops decipiens. The biomass of major zooplankton organisms, including Daphnia and rotifers, increased during the period covered in this study.3. Autocorrelation coefficients have confirmed the existence of recurrent seasonal patterns for both chemical and biological variables.4. Daphnia, calanoid copepods and rotifers showed clear and recurrent seasonal patterns. Cyclopoid copepods and Diaphanosoma had temporal patterns more affected by long‐term trophic changes. Other organisms such as Moina, Ceriodaphnia and Bosmina exhibited high temporal variability with no recurrent patterns.5. There was a strong and positive correlation between total phosphorus and Daphnia biomass. Seasonal patterns of this cladoceran were also inversely associated with sestonic C:P ratios. Thus, Daphnia may be limited primarily by phosphorus and not by total food availability as expressed by particulate carbon.6. This study suggests that increasing eutrophication has changed the structure as well as seasonal patterns of the zooplankton community in Pampulha Reservoir.