Prairie Remnants Research Articles

Restorative recreation: One landowner's experience restoring a cedar‐infested native prairie remnant in Iowa's Loess Hills

Abstract Over 80% of pre‐settlement Iowa was a prairie landscape, but conversion and neglect has reduced that to less than 0.1% of the original 30 million acres (12 million hectares). Much of the remaining remnant prairie lies within the Loess Hills landform in western Iowa. Here I describe my experience as a landowner identifying and restoring a native Loess Hills prairie remnant overtaken by Eastern redcedar (Juniperus virginiana). Archived aerial photographs confirm its native remnant status and have been used to monitor restoration progress. Both shearing and hand clearing of cedars have been undertaken at this site, providing an opportunity to compare patterns of ecosystem recovery within one remnant prairie. I find that cedar shearing, though effective at rapidly clearing a large swath of land, was associated with greater abundance of weedy and woody species compared to hand clearing that occurred more gradually over a longer period of time. However, consistent, targeted herbicide application to woody growth, as well as two prescribed fires, have led to good recovery and stabilization of the grassland with minimal annual recruitment of new brush. Photographs of faunal associations with Pasque flowers (Anemone patens) provide an indicator of the biodiversity on this prairie and suggest Pasque flowers, due to their abundance at this site, function as a keystone species that drives early spring ecosystem productivity. Based on my experience, I introduce a paradigm called restorative recreation to provide a framework for integrating ecosystem restoration activities with outdoor recreation to maximize benefits to personal health and well‐being, while improving the landscape for other species that share our common home.

A comparison of plant communities in restored, old field, and remnant coastal prairies

Temperate grasslands are experiencing worldwide declines due to habitat conversion. Grassland restoration efforts are employed to compensate for these losses. However, there is a need to better understand the ecological effects of grassland restoration and management practices. We investigated the effects of three different grassland management regimes on plant communities of coastal prairie ecosystems in southwest Louisiana (the United States). We compared old fields, prairie remnants, and restored prairies. Coastal prairies are a unique type of grassland historically present across southeast Texas and southwest Louisiana. Old fields represent former coastal prairie habitats allowed to revegetate naturally without active management. Remnant coastal prairies are small, isolated patches of comparatively intact prairie. Restored coastal prairies have been actively restored by planting native coastal prairie vegetation and managed with prescribed burning, mowing, and/or removal of invasive non‐native species. Our work was conducted in three old fields, four remnants, and four restored prairies. Old fields were dominated by non‐native species with low conservation value, whereas remnant prairies were dominated by native species with high conservation value. Remnants had a mean species richness of 75 species per site, which is higher than most other tallgrass prairie ecosystems in North America. Restored sites were dominated by native species with high conservation value, although the composition differed between restored and remnant sites. Collectively, our results: (1) reinforce the importance of identifying and preserving remnant coastal prairies; and (2) show that restoration of degraded coastal prairies is a viable strategy for supporting the persistence of these unique grassland ecosystems.

A chronosequence of soil health under tallgrass prairie reconstruction

Soil health changes induced by prairie reconstruction (cultivated fields to tallgrass prairie) were assessed in Central Missouri within sites representing a chronosequence of 0, 2, 3, 4, 6, 9, 10, 11, 12, and 13-yr post-reconstruction. In addition, a nearby remnant native prairie, two long-term reconstructed prairies (~25 and ~57-yr post-reconstruction), and a biofuel prairie 9-yr post-reconstruction were evaluated for comparative purposes. From 0 to 8-yr, prairie reconstruction increased soil aggregation, total soil organic carbon (SOC), total nitrogen (TN), active C and N (permanganate oxidizable C and total protein), and mineralizable C and N (soil respiration and potentially mineralizable nitrogen), becoming more similar to levels in the remnant prairie. Further, four enzymes involved in the cycling of C (β-glucosidase), N (β-glucosaminidase), P (acid phosphatase), and S (arylsulfatase) demonstrated amplified activities within samples collected to a depth of 15-cm. Over time, the ratios of active C to SOC and active N to TN declined, reflecting the conversion of active C/N pools into more stable C/N pools due to continued organic inputs and increased microbial activity. In contrast, from 8- to 13-yr post-reconstruction, the number of these same soil health indicators declined, which may be attributed to historical land use, the improvement of prairie reconstruction and management strategies, and ecological processes related to succession. Overall, prairie reconstruction holds great potential for soil health restoration in degraded agricultural landscapes, and further study is needed to understand how historical land use and prairie reconstruction practices affect soil health and ecological resilience.

Effects of Natural Habitat Loss and Edge Effects on Wild Bees and Pollination Services in Remnant Prairies

Habitat loss and edge effects resulting from habitat fragmentation are key processes implicated in the decline of bee populations globally. Their effects on wild bees and their pollination services in natural ecosystems are poorly understood, particularly in North American prairies. Our objectives were to determine whether natural habitat loss and edge effects affect bee abundance and pollination services in the Northern Great Plains. We sampled bee abundance and pollination services along transects beginning at road or tree edges in grasslands located in Manitoba, Canada. We measured bee abundance using pan traps, and pollination services using seed-set of Brassica rapa (L.) (Brassicales: Brassicaceae) phytometers. We collected local-scale habitat data by measuring occurrence of flowering species, vegetation type, and vegetation structure, and we measured habitat amount at 1-km radii using GIS analysis of landscape cover. Increasing amounts of habitat loss resulted in declines in bee abundance, and sometimes in pollination services. Results varied with bee life-history: proximity to road edges negatively affected social bees, and litter depth had negative effects on below- ground-nesting bees. Surprisingly, few effects on bees led to corresponding impacts on pollination services. This suggests that conservation of intact natural habitat across the northern Great Plains is important for maintaining resilient and diverse bee communities, but that efforts to conserve bee populations cannot be assumed to also maintain all associated pollination services.

Competition for pollination and isolation from mates differentially impact four stages of pollination in a model grassland perennial

Abstract Species that persist in small populations isolated by habitat destruction may experience reproductive failure. Self‐incompatible plants face dual threats of mate‐limitation and competition with co‐flowering plants for pollination services. Such competition may lower pollinator visitation, increase heterospecific pollen transfer and reduce the likelihood that a visit results in successful pollination. To understand how isolation from mates and competition with co‐flowering species contribute to reproductive failure in fragmented habitat, we conducted an observational study of a tallgrass prairie perennial Echinacea angustifolia. We quantified the isolation of focal individuals from mates, characterized species richness and counted inflorescences within 1 m radius, observed pollinator visitation, collected pollinators, quantified pollen loads on pollinators and on Echinacea stigmas, and measured pollination success. Throughout the season, we sampled 223 focal plants across 10 remnant prairie sites. We present evidence that both co‐flowering species and isolation from mates substantially limit reproduction in Echinacea. As the flowering season progressed, the probability of pollinator visitation to focal plants decreased and evidence for pollen‐limited reproduction increased. Pollinators were most likely to visit Echinacea plants from low‐richness floral neighbourhoods with close potential mates, or plants from high‐richness neighbourhoods with distant potential mates. Frequent visitation only increased pollination success in the former case, likely because Echinacea in high‐richness floral neighbourhoods received low‐quality visits. Synthesis. In Echinacea, reproduction was limited by isolation from potential mates and the richness of co‐flowering species. These aspects of the floral neighbourhood influenced pollinator visitation and pollination success, although conditions that predicted high visitation did not always lead to high pollination success. These results reveal how habitat modification and destruction, which influence floral neighbourhood and isolation from conspecific mates, can differentially affect various stages of reproductive biology in self‐incompatible plants. Our results suggest that prairie conservation and restoration efforts that promote patches of greater floral diversity may improve reproductive outcomes in fragmented habitats.

Habitat-scale heterogeneity maintains fungal endophyte diversity in two native prairie legumes

ABSTRACT The assembly of fungal endophyte communities within plants depends on the complex interactions of fungal taxa, their host plants, and the abiotic environment. Prairie plant communities provide a unique avenue to explore the interplay of biotic and abiotic factors affecting endophyte communities, since the historical distribution of prairies spans a broad range of temperature and precipitation, while the distances between small fragments of contemporary prairie communities may challenge the dispersal capabilities of these otherwise ubiquitous fungi. We sampled foliar fungal endophytes from two native prairie legumes, purple and white prairie clovers (Dalea purpurea and D. candida), in 17 remnant prairie sites across Minnesota in order to evaluate the relative contributions of abiotic factors, host species, and dispersal limitation to the diversity and structure of these communities. We found that similarity of communities was significantly associated with their location along a temperature and precipitation gradient, and we showed a distance-decay relationship that suggests dispersal limitations only over very large spatial scales. Although the effect of host species was small relative to these other factors, the two Dalea species maintained distinct communities within sites where they co-occur. Our results illustrate the capacity of many of these endophyte taxa to disperse over large distances and across heterogeneous biotic and abiotic environments and suggest that the interplay of biotic and abiotic factors maintains high diversity observed in endophyte communities.

Plant Community Shifts in Response to Fire and Bison in a Restored Tallgrass Prairie

To redress prairie loss, managers seed former agricultural lands and reinstate key ecosystem processes with prescribed fires and reintroduction of native grazers to create restored prairies. Bison (Bison bison) were almost hunted to extinction in the late 1800s but are recovering and have recently been reintroduced to restored prairies for their ecosystem engineering roles. The effects bison and fire have on remnant (never-plowed) prairie vegetation are well documented. However, there is little known about how bison will impact plant communities in restored prairies. These effects are most likely driven by reduction of competition from grasses and increased heterogeneity from disturbance. This study aimed to quantify bison impacts on vegetation in a chronosequence of restored prairie with varied burning regimes. We found that plant diversity decreased with restoration age and did not differ among either grazing or fire treatments. However, grazed sites displayed more variation within plant community composition. Older restorations tended to be more similar in species and functional group composition in comparison to younger sites. Although the roles of bison and fire were not as important in these first three years after reintroduction as predicted, changes in composition suggest that bison effects may become more apparent in later years as grazing drives different compositional trajectories.

Identifying Potential Connectivity for an Urban Population of Rattlesnakes (Sistrurus catenatus) in a Canadian Park System

In the face of ongoing habitat loss and fragmentation, maintaining an adequate level of landscape connectivity is needed to both encourage dispersal between habitat patches and to reduce the extinction risk of fragmented wildlife populations. In a developing region of southwestern Ontario, Canada, a declining population of Eastern Massasauga rattlesnakes (Sistrurus catenatus) persists in fragmented remnants of tallgrass prairie in an urban park system. The goal of this study was to identify potential connectivity pathways between habitat patches for this species by using a GIS least-cost permeability swath model, and to evaluate the outputs with snake road mortality data. Results identified seven pathways between five core habitat blocks, a subset of which were validated with aerial imagery and mortality data. Four high-ranking pathways intersected roads through or near road mortality hotspots. This research will guide conservation interventions aimed at recovering endangered reptiles in a globally rare ecosystem, and will inform the use of permeability swaths for the identification of locations most suitable for connectivity interventions in dynamic, urbanizing landscapes.

Sex-biased recapture rates present challenges to quantifying population sizes and dispersal behavior of the regal fritillary butterfly (Speyeria idalia)

The range of the regal fritillary butterfly (Speyeria idalia) has declined substantially due to habitat loss, leading to concerns about the long-term persistence of this species and a current assessment for protection under the Endangered Species Act. Understanding population sizes and dispersal patterns will be important for this assessment and implementing future conservation efforts. The regal fritillary is found in several small remnant prairies south of Sedalia, MO, USA and believed to have relatively large and persistent populations. The objective of this study was to conduct a marking study to quantify local population sizes, capture rates, and movements among state owned Conservation Areas. A total of 109 regal fritillaries were marked at three preserves; however, most effort was directed at Friendly Prairie Conservation Area that yielded a population size estimate of 79 individuals. Males were recaptured more often than females, while the population size estimate was sensitive to a small number of female recaptures. Therefore, a long-term monitoring program will be more efficient if it tracks male abundances, resulting in increased statistical power to detect trends. The low recapture rates of females hindered the ability to assess movement patterns, as females of most butterfly species exhibit long-distance dispersal. Movements of at least 2.9 km and 3.4 km for two males demonstrates connectivity among prairie remnants and may be the reason for the persistent population in this region. Increasing prairie habitat in an arrangement to facilitate movement across the landscape should be implemented to benefit the regal fritillary and other prairie habitat-sensitive butterflies.

Reintroduced bison diet changes throughout the season in restored prairie

Grazing as a management tool is often intended to alter plant community dynamics through preferential foraging. Bison diet in the western United States has been well studied, especially in short and mixed grass remnant prairies. However, there is little known about what bison consume in restored and tallgrass prairies. As bison reintroductions are used more commonly in eastern tallgrass prairies, it is important to understand their diet to predict future impacts on prairie plant communities. This study aims to understand bison diet across different seasons, and asks whether diet differs among male and female, and differently aged bison. We used stable isotope analysis to quantify δ13C and δ15N in plants and used a Bayesian isotope mixing model to estimate bison diet. We found bulls relied more heavily on C4 plants and wetland plants than cows, which relied more heavily on forbs, but no differences in diet between ages. Our analysis shows that bison primarily grazed on C4 grasses throughout the late spring and summer. However, bison foraged more on wetland species and forbs in the late summer and fall. This change in diet could have implications for wetland species and habitats, through dung inputs and trampling. The relatively high reliance on forbs for nearly one‐third of bison diet could mean intended impacts of reintroduced bison such as increased plant diversity through preferential grazing on grasses could be dampened. Managers reintroducing bison to restored prairie ecosystems should ensure adequate wetland and forb species, in addition to a mix of grasses.

Abiotic and biotic context dependency of perennial crop yield.

Perennial crops in agricultural systems can increase sustainability and the magnitude of ecosystem services, but yield may depend upon biotic context, including soil mutualists, pathogens and cropping diversity. These biotic factors themselves may interact with abiotic factors such as drought. We tested whether perennial crop yield depended on soil microbes, water availability and crop diversity by testing monocultures and mixtures of three perennial crop species: a novel perennial grain (intermediate wheatgrass—Thinopyrum intermedium-- that produces the perennial grain Kernza®), a potential perennial oilseed crop (Silphium intregrifolium), and alfalfa (Medicago sativa). Perennial crop performance depended upon both water regime and the presence of living soil, most likely the arbuscular mycorrhizal (AM) fungi in the whole soil inoculum from a long term perennial monoculture and from an undisturbed native remnant prairie. Specifically, both Silphium and alfalfa strongly benefited from AM fungi. The presence of native prairie AM fungi had a greater benefit to Silphium in dry pots and alfalfa in wet pots than AM fungi present in the perennial monoculture soil. Kernza did not benefit from AM fungi. Crop mixtures that included Kernza overyielded, but overyielding depended upon inoculation. Specifically, mixtures with Kernza overyielded most strongly in sterile soil as Kernza compensated for poor growth of Silphium and alfalfa. This study identifies the importance of soil biota and the context dependence of benefits of native microbes and the overyielding of mixtures in perennial crops.

Effects of the soil microbiome on the demography of two annual prairie plants.

Both mutualistic and pathogenic soil microbes are known to play important roles in shaping the fitness of plants, likely affecting plants at different life cycle stages.In order to investigate the differential effects of native soil mutualists and pathogens on plant fitness, we compared survival and reproduction of two annual tallgrass prairie plant species (Chamaecrista fasciculata and Coreopsis tinctoria) in a field study using 3 soil inocula treatments containing different compositions of microbes. The soil inocula types included fresh native whole soil taken from a remnant prairie containing both native mutualists and pathogens, soil enhanced with arbuscular mycorrhizal (AM) fungi derived from remnant prairies, and uninoculated controls.For both species, plants inoculated with native prairie AM fungi performed much better than those in uninoculated soil for all parts of the life cycle. Plants in the native whole prairie soil were either generally similar to plants in the uninoculated soil or had slightly higher survival or reproduction.Overall, these results suggest that native prairie AM fungi can have important positive effects on the fitness of early successional plants. As inclusion of prairie AM fungi and pathogens decreased plant fitness relative to prairie AM fungi alone, we expect that native pathogens also can have large effects on fitness of these annuals. Our findings support the use of AM fungi to enhance plant establishment in prairie restorations.

Evaluating Native Bee Communities and Nutrition in Managed Grasslands.

Native pollinators are important for providing vital services in agroecosystems; however, their numbers are declining globally. Bees are the most efficient and diverse members of the pollinator community; therefore, it is imperative that management strategies be implemented that positively affect bee community composition and health. Here, we test responses of the bee and flowering plant communities to land management treatments in the context of grasslands in the upper Midwestern United States, a critical area with respect to bee declines. Twelve sites were selected to examine floral resources and wild bee communities based on three different types of grasslands: tallgrass prairie remnants, ungrazed restorations, and grazed restorations. Total bee abundance was significantly higher in ungrazed restorations than remnants, but there were no significant differences among grasslands in community composition or Shannon diversity. Across the three grassland types we also examined mass and lipid stores as nutritional health indicators in three sweat bees (Halictidae), Augochlora pura, Agapostemon virescens, and Halictus ligatus. Although there were no differences in lipid content, total average bee mass was significantly higher in Ag. virescens collected from ungrazed restorations as compared to remnants. Floral abundance of native and non-native species combined was significantly higher in grazed restorations compared to remnants and ungrazed restorations. However, ungrazed restorations had higher abundance and richness of native flowering ramets. These data suggest that bee abundance and nutrition are driven by high abundance of native flowering plant species, rather than total flowering plants.

Demographic costs and benefits of herbicide‐based restoration to enhance habitat for an endangered butterfly and a threatened plant

Restoring habitat degraded by invasive species is often a primary focus of conservation strategies, yet few studies investigate the effects of invasive species control on multiple at‐risk taxa. Selective herbicides are increasingly used because they can selectively reduce aggressive invasive plant species with the aim of minimizing effects on other taxa within the habitat. We conducted a four‐year experiment to test how annual application of grass‐specific herbicide affected the demography on Fender's blue butterfly (Icaricia icarioides fenderi) and Kincaid's lupine (Lupinus oreganus), two federally protected species which persist in highly degraded prairie remnants in western Oregon, USA. Effects of herbicide application were transitory for the butterfly; reduction of invasive grasses increased fecundity and led to higher annual population growth (λ) at one of two conservation areas in the first season. There were no detectable differences in λ in subsequent seasons—suggesting that treatments caused neither extensive harm nor extensive benefit to the butterfly population. For the lupine, there were no detectable differences in leaf and flower abundance between control and herbicide treatments. However, greater seed production in herbicide plots in the first and third seasons suggests that lupines in herbicide‐treated plots have greater potential reproductive success. While treatments do not have a long‐term benefit to annual population growth for the butterfly, increasing reproductive success of the threatened plant may justify integrating this strategy into restoration plans. Considering the impact of restoration practices on the demography of multiple at‐risk taxa within a community is critical to effective recovery strategies.

Remnant Prairies and Organic Gardens Provide Complementary Habitat for Native Bees Within a Midwestern Urban Matrix

Remnant Prairies and Organic Gardens Provide Complementary Habitat for Native Bees Within a Midwestern Urban Matrix

Inoculation with remnant prairie soils increased the growth of three native prairie legumes but not necessarily their associations with beneficial soil microbes

Restoring the diversity of plant species found in remnant communities is a challenge for restoration practitioners, in part because many reintroduced plant species fail to establish in restored sites. Legumes establish particularly poorly, perhaps because they depend on two guilds of soil microbial mutualists, rhizobial bacteria and arbuscular mycorrhizal (AM) fungi, that may be absent from restored sites. We tested the effect of soil microorganisms from remnant and restored prairies on legume growth by inoculating seedlings of Lespedeza capitata, Amorpha canescens, and Dalea purpurea with soil from 10 restored prairies and 6 remnant (untilled) prairies from southwest Michigan. We generally found support for the hypothesis that restored prairie soils lack microbes that enhance prairie plant growth, although there was variation across species and mutualist guilds. All three legumes grew larger and two legumes (Lespedeza and Amorpha) produced more nodules when inoculated with soil from remnant prairies, suggesting that low quantity and/or quality of rhizobial partners may limit the establishment of those species in restored prairies. In contrast, no legume experienced greater root colonization by AM fungi in remnant prairie soils, suggesting equivalent quantity (but not necessarily quality) of fungal partners in remnant and restored prairie soils. We detected no evidence of spontaneous recovery of the community of beneficial soil microbes in restorations. These results suggest that the absence of rhizobia, a largely overlooked component of prairie soils, could play a strong role in limiting restored prairie diversity by hindering legume establishment. Active reintroduction of appropriate rhizobial strains could enhance prairie restoration outcomes.

Passive Re-colonization of the Spider Assemblage on an Ohio Restored Tall Grass Prairie Compared to Nearby Remnant Prairies and Old Fields

The reconstructed prairie on the Marion Campus of The Ohio State University was established in 1977. Since then restoration has focused on plants. Animals on the site have recolonized without active management. Spider assemblages were sampled in 2000 and compared to those sampled at the time on 2 remnant prairies and 2 old fields. Pitfall traps and sweep nets were used for sampling. In 2000, spiders (n = 1,541) representing 94 species were captured; 91% of these were captured with pitfall traps. The restored Marion Campus Prairie was inhabited by an assemblage of spiders resembling those on nearby remnant prairies and old fields.

Vegetation change over seven years in the largest protected Pacific Northwest Bunchgrass Prairie remnant.

Temperate grasslands are one of the most altered ecosystems on Earth. Consequently, conservation of important characteristics of such ecosystems (e.g., biodiversity) is uncertain even within grasslands that have been protected. Invasion by non-native plants is considered a primary threat to intact grasslands. Here, we evaluated native and non-native vegetation composition change over seven years in the largest Pacific Northwest Bunchgrass remnant. We sampled 124 permanent plots across the Zumwalt Prairie Preserve (northeastern Oregon, USA) twice, seven years apart. With data collected from three grassland community types (xeric prairie, mesic prairie, old fields), we asked: (1) how has species composition changed over time; (2) which species showed the greatest changes in abundance; and (3) how did abundance of Ventenata dubia (the most abundant non-native species) relate to patterns of native and non-native plant abundance? Vegetation composition changed in all three plant communities. Ventenata dubia, an annual non-native grass: (1) became the third most dominant species across the study area; (2) was the only non-native that increased in abundance substantially in all three communities; and (3) was negatively related to native perennial forb cover. Relative cover of non-native species decreased in old fields concomitant with increases in native bunchgrass (Festuca idahoensis) and V. dubia cover. Increased cover of native perennial grasses and non-native annual grasses in old fields were associated with loss of bare ground, but not with reductions in non-native perennial grass cover. Native species dominated in the mesic prairie; however, non-native cover (particularly V. dubia) increased (mean cover increased from 3 to 10%) while mean native perennial forb cover decreased (from 30 to 25%) over time. Continued shifts towards non-native annual grass dominance coupled with potentially declining native perennial forbs, may challenge conservation efforts in one of the last large tracts of Pacific Northwest Bunchgrass Prairie.

Low conservatism of leafhopper communities in remnant and reconstructed prairie sites in a working agroecological landscape

Leafhoppers (Hemiptera: Cicadellidae) form a large, diverse insect family that contributes significantly to trophic interactions and pathogen transmission in grasslands. We compared leafhopper communities of five remnant prairies and five prairie reconstructions in northeastern Kansas, USA. We hypothesized that leafhopper communities would be more conservative (i.e., greater dependence on prairies) and diverse on remnant as compared to reconstructed prairies, and that remnants would support more grass-feeders. We also predicted that sites surrounded by natural/semi-natural landscapes would have greater leafhopper diversity. We found grass feeders represented a higher proportion of leafhoppers on remnants. We found, however, no difference in mean conservatism between remnants and reconstructions, and overall levels of conservatism were lower than other grassland data sets. Mean conservatism was greater in the mid-summer than in early summer, where migratory pests such as Macrosteles were abundant. Although remnants and reconstructed prairies didn’t differ in leafhopper diversity, diversity increased with forb percent cover. The amount of natural and semi-natural land cover around sites had complex relationships with leafhopper diversity and composition. For future studies, we encourage coordinated studies on leafhopper communities across the tallgrass prairie region, with a particular focus on conservatism. Deciphering the effect of survey times, and grassland size and management, on prairie-dependent leafhoppers is important. We predict that large numbers of migratory species may lead to greater homogeneity in leafhopper communities across sites early in the season. Over time, site types and landscape features may progressively filter species and amplify local specialists, resulting in more late-season variation among sites.

A Comparison of Adult Butterfly Communities on Remnant and Planted Prairies in Northeast Iowa

Adult butterfly abundance and diversity was compared in four remnant and four planted tallgrass prairies in Northeast Iowa. Butterfly surveys were conducted at approximately three week intervals over the summer of 2015. Researchers used a modified “Pollard Walk” technique following a meandering transect, and butterfly sightings recorded using the Unified Butterfly Recorder (UBR) Android app. If a butterfly was observed nectaring on a flower, the species of flower was recorded. Floral resource availability, plant species richness and percent cover were measured in each prairie. Although planted prairies contained significantly greater floral resources than remnant prairies, there was no significant difference in floral plant species richness between remnants and plantings. Remnant and planted prairies did not differ in butterfly abundance, but remnant prairies had significantly greater butterfly species richness than planted prairies. These results provide valuable information on the current status of butterflies in each prairie type in northeast Iowa, and can be used in directing future land management and conservation work.