ObjectivesThe aim of this study was to validate the Acoustic Voice Quality Index (AVQI) version 3.01 in the Turkish-speaking population. Materials and methodsConcatenated voice samples of the sustained vowel [a:] and continuous speech were collected from 127 dysphonic and 128 normophonic participants. The auditory-perceptual evaluation was performed by five experienced raters using the Grade parameter of the GRBAS scale. Rater reliability, concurrent validity, diagnostic accuracy, and differences between normophonic and dysphonic groups were analyzed for the AVQI version 3.01. ResultsThe number of syllables for the standardized reading text with the concatenation of the voiced parts lasting around 3 seconds (mean=3.84s) was 36. The ICC values of intra-rater reliability of G scores of five raters were excellent (mean ICC=0.934), and of inter-rater reliability they varied between moderate and excellent (mean ICC=0.786). AVQIv3 demonstrated a high diagnostic accuracy with AUC = 0.906 in identifying disrupted versus normal voice quality. With sensitivity of 80% and specificity of 94%, AVQIv3 = 2.345 was the cut-off point that differentiated most accurately between normophonic and dysphonic voices in Turkish. ConclusionAVQIv3 is an ecologically valid tool for objective differentiation between dysphonic and normal voices in the Turkish language.