Reliability Test Research Articles

The Turkish version of the brief Michigan hand outcomes questionnaire: cross-cultural adaptation, validity, and reliability testing

The Turkish version of the brief Michigan hand outcomes questionnaire: cross-cultural adaptation, validity, and reliability testing

A simplified pyrazinamidase test for Mycobacterium tuberculosis pyrazinamide antimicrobial susceptibility testing

ABSTRACT Pyrazinamide (PZA) is an important first-line drug for tuberculosis (TB) treatment by eradicating the persisting Mycobacterium tuberculosis complex (MTBC). Due to cost and technical challenges, end TB strategies are hampered by the lack of a simple and reliable culture-based PZA antimicrobial susceptibility testing (AST) for routine use. We initially developed a simplified chromogenic pyrazinamidase (PZase) test in the TB reference laboratory using a training set MTBC isolates with various drug-resistant profiles, and validated its performance using consecutive BACTEC MGIT 960 (MGIT)-culture-positive culture in 10 clinical laboratories. The pncA gene Sanger sequencing results were used as the reference, and compared to the MGIT-PZA AST. Differential diagnosis of Mycobacterium bovis was conducted using patented in-house real-time PCR. Of the 106 training isolates, the PZase test and MGIT-PZA AST showed 100.0% and 99.1% concordance as compared to Sanger sequencing, respectively. We found 32.1% (34/106) isolates harbored pncA mutations, including one isolate with silent mutation S65S. For validation, 1,793 clinical isolates were tested including 150 duplicate isolates from specimens of the same cases and 16 isolates with uncharacterized drug resistance (UDR)-associated mutations. Excluding duplicated and UDR isolates, we identified 2.6% (43/1,627) PZA-resistant isolates, including 1.3% (21/1,627) M . bovis isolates. The kappa values were 0.851–1.000. In addition, the accuracy of the PZase test conducted by 10 laboratories was 98.5%–100.0%. Our simplified PZase test demonstrated high concordance with Sanger sequencing and MGIT-PZA AST. Integrating the PZase test into routine first-line AST is effortless and represents an improvement in laboratory services for ending TB. IMPORTANCE We developed and validated a simple pyrazinamidase (PZase) test for pyrazinamide (PZA) antimicrobial susceptibility testing (AST). Our results demonstrated that the PZase test had high agreement with the pncA gene sequencing and MGIT-PZA AST. Integrating PZase test into routine AST is effortless and represents an improvement in laboratory services for ending TB.

Cricket players’ perceptions of playing on artificial turf

ABSTRACT With the recent introduction of artificial turf outfield playing surfaces in community-level cricket, this paper presents the physical performance and injury-related perceptions of male, senior cricketers. Following validity and reliability testing, 43 cricketers from two clubs completed a questionnaire comprising three sections: demographic information; individual performance; and injury risk and severity. Descriptive variables were computed, and chi-square tests were conducted to identify differences in perceptions based on the field type that players were exposed to and their injury status. Most players preferred artificial turf for training (53.7%) and wet/rainy conditions (64.1%), but natural grass for competitive games (67.4%) and dry conditions (69.0%). There were significant differences in perceptions for game speed (p < 0.001), the need to be fitter (p = 0.015), and the overall physical performance quality based on injury status (p = 0.017) and field (p < 0.021). Skin injuries were the only injuries that were reported as more likely on artificial turf. In general, players did not feel that the technical and physical performance aspects of cricket were affected when playing on artificial turf compared to natural grass. However, the field type that players were exposed to significantly influenced their perceptions and highlights the importance of artificial turf composition for future adoption in cricket.

An innovation in the method of assessing the fire resistance of a reinforced concrete girderless ceiling of a building

The article presents a new method for assessing the fire resistance of monolithic girderless non-accumulating floors of buildings and structures. The essence of the method lies in the fact that thermal (fire) tests of the floor are carried out without destruction, according to a set of single indicators of the quality of working fittings and structural concrete of continuous slabs. The use of the method makes it possible to determine the fire resistance of an uncut reinforced concrete slab of a girderless ceiling of a building without full-scale fire exposure, increases the reliability of statistical quality control and non-destructive testing, reduces economic costs.

UV‐Induced Degradation of Industrial PERC, TOPCon, and HJT Solar Cells: The Next Big Reliability Challenge?

With the surge of UV‐transparent module encapsulants in the photovoltaic industry aiming to boost quantum efficiency, modern silicon solar cells must now inherently withstand UV exposure. UV‐induced degradation (UVID) of nonencapsulated laboratory and industrial solar cells from several manufacturers is investigated. Passivated emitter rear contact (PERC), tunnel oxide passivating contact (TOPCon), and silicon heterojunction (HJT) cells can suffer from severe implied voltage degradation (&gt;20 mV) after UV exposure relating to 3.8 years of module installation in the Negev desert. Front UV‐exposure causes more performance loss than an equal rear dose. This is connected to a higher UV transmission of the cell layers outside the bulk, indicating the photons need to reach the silicon surface to induce damage. Current–voltage measurements of the TOPCon groups most sensitive to UV degradation show more than 7%rel efficiency loss with the Voc as the main contributor. For two TOPCon groups, dark storage for 14 days after UV exposure causes an additional voltage drop on a similar scale as the UV damage itself, impeding straightforward reliability testing. UVID appears to be a complex process general to all dominant cell architectures with the potential to diminish efforts in efficiency optimization within only a few years of field employment.

Measurement of Science Literacy Skills of Elementary School Teacher Education Students: Development and Validity Testing of Assessment Instruments

This study aims to develop and test the validity and reliability of a scientific literacy instrument for students of the Elementary School Teacher Education (PGSD) Program. The instrument was designed to measure three dimensions of scientific literacy: content knowledge, procedural knowledge, and epistemic knowledge, focusing on Earth and Space Science topics. A total of 18 questions were constructed based on indicators that assess the ability to explain scientific phenomena, evaluate scientific investigations, and interpret scientific data. The instrument was tested on 33 PGSD students at Universitas Sebelas Maret (UNS) and analyzed using the Content Validity Index (CVI) and reliability tests via SPSS software. The results indicated that most items were valid, with an S-CVI/Ave value of 0.981 and an S-CVI/UA value of 0.8333. However, one item was found invalid with an I-CVI score of 0.67. The reliability test showed that some questions had a good level of reliability, while others required revision due to low reliability. Additionally, analysis using the Item Response Theory (IRT) revealed that the questions varied in difficulty and discrimination, with most questions having positive discrimination values and a range of difficulty levels. Overall, the instrument was found to be valid and reliable for assessing the scientific literacy of PGSD students, although some questions need revision to improve consistency and accuracy.

A Multi-Channel Handheld Diagnostic Device Based on Green Biomimetic Material for Point-of-Care Testing of Vitamin C in Human Fluids.

In the era of "Great Health", maintaining an appropriate level of vitamin C is crucial for human health as it directly impacts the proper functioning of the immune system. In this study, a handheld nonenzymatic electrochemical sensor based on biomimetic material prussian blue functionalized polydopamine is proposed for point-of-care testing (POCT) of vitamin C in human fluids. Particularly, Prussian blue, known as a bio-antidote, and polydopamine, a main component of cuttlefish ink, ensure the safety and reliability of home testing. The experimental results quantitatively demonstrate that this electrochemical POCT sensor enables rapid and accurate determination of vitamin C with a wide linear detection range from 0.2 to 200µm, a low limit of detection at 0.03µm, and high sensitivity at 0.33µAµm-1cm-2. More importantly, the performance evaluation using human urine and saliva samples confirms satisfactory accuracy and recovery rates for vitamin C determination by this electrochemical POCT sensor. Thus, the proposed handheld electrochemical POCT sensor holds potential utility as an affordable tool for VC determination and home-based healthcare.

Unveiling the Impact of Emotional Regulation on Academic Procrastination

This study examines how consumer perceptions of the Skintific brand, product quality, and pricing influence product selections among 110 FBHIS students at Muhammadiyah University of Sidoarjo. Using online questionnaires and quantitative analysis methods, including validity and reliability tests, multiple linear regression, and t-tests, the research reveals significant relationships between brand image, product quality, pricing, and consumer purchasing decisions. The findings emphasize the importance of these factors in shaping consumer behavior, particularly among university students, and offer insights for skincare companies targeting this demographic to enhance brand image, maintain product quality, and offer competitive pricing strategies. Highlight: Quantitative Analysis: Validity, reliability tests, regression for consumer behavior research. University Sample: Investigated FBHIS students' purchasing choices at Sidoarjo University. Strong Associations: Brand image, product quality, pricing linked to consumer decisions. Keywoard: Consumer perceptions, Skintific brand, Product quality, Pricing, Decisions

QUALIDADE PARA GARANTIR A CONFIABILIDADE DOS RESULTADOS DOS TESTES DA COVID 19

Objective: To analyze and identify the main quality criteria essential to ensure the reliability of COVID-19 test results performed in clinical laboratories. Literature Review: Ensuring the quality of COVID-19 testing is vital for the effectiveness of pandemic control strategies. This involves meticulous care with the selection and implementation of tests, the formation and training of teams, and adherence to standards and good practices. Only with an ongoing commitment to quality and accuracy can we ensure that test results reflect the true situation of COVID-19 infection and aid in public health decision-making.Conclusion: To ensure the reliability of COVID-19 test results, it is essential to take a comprehensive approach that encompasses test accuracy, sample quality, rigorous validation, staff qualification, and transparency.

Reliability of Sensorimotor Control Tests in Individuals with Adolescent Idiopathic Scoliosis

Background: The presence of sensorimotor control deficits in adolescent idiopathic scoliosis compared to typically developed adolescents is supported by the literature but lacks reliability studies for assessment in this population. This study aimed to assess the reliability of eight sensorimotor control tests, in terms of static and dynamic balance, joint position sense (JPS) tests of the extremities and the spine, and a functional upper extremity proprioceptive test in adolescent idiopathic scoliosis subjects. Methods: Sixty adolescent idiopathic scoliosis subjects were divided into four groups. Each group underwent two tests by the same examiner, repeated at 15 min intervals. Reliability was measured using the intraclass correlation coefficient (ICC), standard error of measurement (SEM), and smallest detectable difference (SDD). Results: The results showed high reliability for the upper extremity functional proprioception test, for the dynamic and static balance test, and for the spinal lateral flexion joint position sense test in both directions. On the other hand, the shoulder external rotation, knee extension, elbow flexion, and spinal flexion joint position sense tests demonstrated poor reliability in adolescent idiopathic scoliosis subjects. Conclusions: Therapists are recommended to use the functional upper extremity proprioception test, the Fukuda test, the static balance test using a force footplate, and the spinal lateral flexion joint position sense test for assessing treatment progress in this population.

Performance evaluation of the Specific Reveal system for rapid antibiotic susceptibility testing from positive blood cultures containing Gram-negative pathogens.

Rapid antimicrobial drug administration is crucial for the efficient treatment of sepsis or septic shock, but empirical therapy is limited by the increasing prevalence of multidrug-resistant bacteria. Thus, rapid and reliable antimicrobial susceptibility testing (AST) is needed to start appropriate antimicrobial drug administration as quickly as possible. In the present study, we evaluated the performance of the Reveal rapid AST system. From February to April 2021, 102 positive blood culture bottles (BCBs) from hospitalized patients with bacteremia caused by Gram-negative bacteria were included in the study. All isolates were tested by the Reveal system directly from the positive BCBs in comparison to the DxM MicroScan WalkAway. Essential agreement (EA) and category agreement (CA) were high with 98.5% and 97.1%, respectively. We also determined the susceptibility of 10 highly resistant CDC & FDA AR strains in duplicate. Here, EA was 99.6% and CA 97.9%. The average time to result by Reveal was 5.4 h ± 1.2 h compared to an average of 16 h by DxM MicroScan WalkAway for clinical strains and 3.8 h ± 1.2 h for more resistant CDC & FDA AR strains. Susceptibility determination with the Reveal rapid AST system directly from positive BCBs is for the frequently represented bug-drug combinations a reliable and accurate approach, meeting the European ISO guideline for the performance of AST systems. Moreover, AST directly from blood cultures performed with the Reveal system saves time when compared to the conventional AST, as no subculturing is needed and time to result is very short.

Application of Hierarchical Bayesian Models for Modeling Economic Costs in the Implementation of New Diagnostic Tests

The COVID-19 pandemic has highlighted the need for reliable and rapid diagnostic tests to control the spread of infection. The introduction of new rapid antigen tests often goes in tandem with the limited data availability, making it challenging to assess their performance at the initial phase of the pandemic. Sensitivity and specificity, the key performance characteristics provided by manufacturers, are typically derived under laboratory conditions and may not accurately reflect the tests' performance in field settings. We use the hierarchical Bayesian model to obtain their realistic estimates in real world conditions and show how it may be used in situations in which new tests with limited history are presented on the market. Proposed methodology allows for the efficient information pooling, thereby improving on the accuracy of parameter estimates for new tests. The results suggest that the application of hierarchical model on the Czech data led to a considerabile reduction in uncertainty associated with the parameter estimates as well as with potential economic cost implied by false positive test results. The model can thus assist in better informed decision-making and financial planning of both the government and corporations.

Concrete Infrastructure: Recent Advancements and Needs with a Focus on North America

This letter provides an overview of the continent’s diversity in geography and climatic exposure and the impact of chlorides on reinforced concrete structures in North America. Several research needs are identified, including those that arise as specifications begin to change from prescriptive to performance-based approaches. Further, the widespread changes in material compositions or chloride exposures, require important changes to specifications, design practices, or maintenance procedures. Related to reducing carbon emissions, there is a need to reduce clinker content in concrete mixtures, increase the use of novel cementitious and supplementary cementitious materials (SCM), and to understand the durability of such concretes. The following research efforts from a North American context are warranted: (i) investigating the long-term durability of novel cement and SCM systems, including non-Portland cement-based materials and those made with CO2 mineralization used to meet carbon emission targets; (ii) understanding climate change impacts of temperature and sea levels, including flood impact, on chloride exposure and chloride-induced corrosion; (iii) developing rapid and reliable tests to estimate durability in practice, particularly for scaling, freeze-thaw, salt damage, and chloride-induced corrosion; and (v) developing better understanding of the short and long-term implications of changes in constituent materials and exposure.

Real‐time hybrid simulation for investigating the seismic response of a bridge isolated with lead rubber bearings

AbstractThis study investigates the seismic response of lead rubber bearings (LRBs) under constant and time‐varying axial forces using real‐time hybrid simulation (RTHS). Although shake table testing can provide realistic seismic responses, it is often expensive and quite challenging for large‐scale structures. RTHS, however, offers a cost‐effective alternative by experimentally testing only the structural component of interest while analytically modeling the remaining structure. With the use of an advanced real‐time force control method, this study implemented RTHSs for a bridge isolated with two LRBs, where the LRBs are subjected to various constant axial forces due to self‐weight as well as time‐varying axial forces induced by vertical ground motions. The lateral response of LRBs was found to be significantly influenced by the magnitude of constant axial force, highlighting the importance of incorporating the effect of axial force due to self‐weight into numerical simulations. Additionally, it is crucial to satisfy the axial force boundary condition when conducting RTHS or cyclic loading tests to obtain more reliable test results. On the other hand, the time‐varying axial force generated by the vertical vibration of the bridge due to vertical ground motions has a limited impact on the lateral response of LRBs.

To evaluate the reliability of Urea and Creatinine values in vaginal wash fluid for the diagnosis of PROM

Background &amp; Methods: The aim of the study is to evaluate the reliability of urea and creatinine values in vaginal wash fluid for the diagnosis of PROM. Study performed on pregnant women in the third trimester between 28 and 37 weeks of gestation with history of possible leakage of amniotic fluid at tending in the department of Obstetrics and Gynaecology. Results: Total 50 subjects were recruited. Group 1 is PROM confirmed 25 subjects . Group 2 were controls and in each group 25 subjects were allotted. The comparison of co-morbidity between study groups in vaginal wash fluid to diagnosis the high rupture of membrane. Around 24% of subjects had co-morbidity in PROM confirmed category. Conclusion: In PROM confirmed subject urea level was 9.99 ± 4.16 and in controls it was around 4.12 ± 2.65. This shows that both PROM confirmed and suspected subjects had increased urea level than the control group. Thus urea and creatinine levels in vaginal wash fluid serves as an excellent diagnostic marker in diagnosing premature rupture of membrane. In conclusion, our study demonstrated that the measurement of vaginal fluid urea and creatinine is a simple and reliable test for diagnosis of PROM.

Criterion-Related Validity and Reliability of the Six-Minute Run Walk Test Among Children: Findings from a Systematic Review and Meta-Analysis

ABSTRACT The aim of this study is to examine the available scientific evidence on the reliability and criterion validity of 6-minute run walk field-based test when administered to children and adolescents. Systematic searches were performed in three electronic databases (MEDLINE/PubMed, SPORTDiscuss and Scopus) from their inception until February 2024, with an update on August 15, 2024. Studies were considered eligible if they provided information on the criterion-related validity and/or reliability of the 6-minute run walk test when conducted by children (aged 12 years and under) and/or adolescents (aged 13 to 17 years). To examine reliability and validity, two separate meta-analyses were implemented. In the meta-analysis focused on criterion-related validity, the five studies included indicated a fair validity (r = 0.56 [95% CI: 0.44–0.66]) and high homogeneity (I2 0%). In the meta-analysis focused on reliability, the pooled data from the five studies employing Pearson’s correlation coefficient (n = 585) revealed strong reliability values (r = 0.82 [95% CI: 0.75–0.87]), albeit with a moderate level of heterogeneity (I2 71% prediction interval 0.55–0.94). The evidence suggests that the 6-minute run walk field-based test demonstrated fair criterion-related validity and strong test-retest reliability when conducted with children and adolescent.

MAIA and Humphrey Perimetry Differ in Their Estimation of Homonymous Visual Field Defects.

To assess whether the Humphrey Visual Field Analyzer (HFA) and the Macular Integrity Assessment instrument (MAIA) provide equivalent estimates of visual deficit size, severity, and progression in cortically blinded participants. Reliable, monocular 10-2 HFA and MAIA fields were collected at baseline, and after a blind-field training intervention (n = 54) or no intervention (n = 6) in adult participants with occipital strokes. Binocular HFA and MAIA mean sensitivities (MS) were first computed, before creating binocular maps of visual sensitivity for each perimetry system to calculate deficit areas, using a unitary, published, less than 10 dB criterion to define blindness. We contrasted HFA/MAIA MS and deficit area at individual study visits, together with change in these measures between visits. At individual visits, MS and deficit areas were well-correlated, but there were systematic differences between machines, greater for the intact than impaired hemifields, with the MAIA overestimating areas of visual deficit relative to the HFA. Between visits, the two perimeters' assessment of change in MS was correlated, but change in the deficit area was not, despite good test reliability. Reliable HFA and MAIA tests produce well-correlated but systematically offset estimates of visual sensitivity and deficit area in patients with homonymous field defects, which can impact assessment of progression. Cortically blinded patients exhibited comparable changes in sensitivity (spontaneous and training induced) when assessed using Humphrey and fundus-controlled MAIA perimetry. However, the photopic vs. mesopic nature of these tests impacted participant performance and suggests that custom, machine-specific criteria are needed to comparably define blindness on both systems.

Validation of the Psychometric Properties of the Conflict Resolution Styles Inventory in the University Population

This study aimed to validate the psychometric properties of the Conflict Resolution Styles Inventory (CRSI) within the context of the university population in Ecuador. The CRSI measures how individuals manage interpersonal conflicts, a critical skill for university students. A sample of 746 university students from various institutions across Ecuador participated in the study. The CRSI, which categorizes conflict resolution styles into five types (competing, avoiding, accommodating, collaborating, and compromising), was translated and culturally adapted for the Ecuadorian context. Psychometric analyses, including factor analysis and reliability testing, were conducted to assess the validity and reliability of the inventory. The factor analysis supported the five-factor structure of the CRSI, confirming that the inventory is suitable for measuring distinct conflict resolution styles in this population. The inventory showed good internal consistency, with Cronbach’s alpha values exceeding 0.70 for all subscales. Additionally, the test–retest reliability indicated stability over time. The validated CRSI provides a robust instrument for understanding and improving conflict resolution skills among university students in Ecuador, contributing to better interpersonal relationships and academic environments.

Testing in Football: A Narrative Review

Football clubs regularly test and monitor players, with different approaches reflecting player age and competitive level. This narrative review aims to summarise justifications for testing and commonly used testing protocols. We also aim to discuss the validity and reliability of specific tests used to assess football players and provide a holistic overview of protocols currently used in football or those demonstrating potential utility. The PubMed, SportDiscus, and Google Scholar databases were screened for relevant articles from inception to September 2024. Articles that met our inclusion criteria documented tests for several purposes, including talent identification or the assessment of growth/maturation, physiological capacity, sport-specific skill, health status, monitoring fatigue/recovery, training adaptation, and injury risk factors. We provide information on specific tests of anthropometry, physical capacity, biochemical markers, psychological indices, injury risk screening, sport-specific skills, and genetic profile and highlight where certain tests may require further evidence to support their use. The available evidence suggests that test selection and implementation are influenced by financial resources, coach perceptions, and playing schedules. The ability to conduct field-based testing at low cost and to test multiple players simultaneously appear to be key drivers of test development and implementation among practitioners working in elite football environments.

Adaption of a trigger tool to identify harmful incidents, no harm incidents, and near misses in prehospital emergency care of children.

The emergency medical service (EMS) addresses all chief complaints across all ages in various contexts. Children in EMS present a particular challenge due to their unique anatomical and physical properties, which require specific training that EMS clinicians often report lacking. This combination exposes children to incidents threatening patient safety. The most common method to highlight incidents is the incident reporting system. Studies have shown underreporting of such incidents, highlighting the need for multiple methods to measure and enhance patient safety in EMS for children. Thus, the aim of this study was to modify and adapt the current Ambulance TT for road-based EMS (ATT) to a pediatric version (pATT) with a guide containing definitions of triggers. The adaption of the ambulance trigger tool to a version suitable for children followed a stepwise manner, including (1) a review of previous literature to pinpoint areas of risk regarding patient safety among children in EMS. (2) Three sessions of expert panel discussions via video meetings were held to evaluate each trigger of the ATT in terms of clinical relevance, comprehensibility, language and areas of risk regarding patient safety among children in EMS. (3) Clinical use of the pATT along with Retrospective Record Review (RRR). (4) Calculation of Item-level validity index and positive predictive value (PPV) for each trigger. (5) calculate inter-rater reliability between two independent record reviewers. The literature search revealed 422 respective 561 articles in Cinahl and Medline where headlines and abstracts were read to identify areas posing risks to patient safety in EMS for children. During the structured discussions, one trigger was added to the existing 19 derived from the ATT, and the trigger definitions were modified to suit children. The three most common triggers identified in the 900 randomly selected records were deviation from treatment guidelines (63.9%), incomplete documentation (48.3%), and the patient is non conveyed after EMS assessment (41.1%). The positive triggers were categorized into near miss (54.6%), no harm incident (5.8%), and harmful incident (0.4%). Inter-rater reliability testing showed excellent agreement. This study demonstrates the adaptation of an existing trigger tool (ATT) to one suitable for children. It also shows that the trigger tool, along with retrospective record review, is a feasible method to evaluate patient safety in EMS, thus complementing existing methods.