Reliability Test Research Articles

Depictions of Strabismus in Children’s Animated Films

BACKGROUND: Strabismus is known to negatively affect patients' self-confidence and ability to interact with society. Strabismus is commonly depicted in animated films marketed to children, potentially influencing the perception of strabismus by young impressionable audiences. METHODS: We reviewed all animated films released by Walt Disney Animation Studios from 1989 to 2022 and all films released by Pixar, DreamWorks, and Studio Ghibli before 2023 and identified all characters with strabismus. Five raters each independently performed a character trait analysis of all characters with strabismus, and a sample proportion test was used for statistical comparison of these traits. RESULTS: One hundred twenty five movies met inclusion criteria. We identified 46 characters with strabismus, with at least 1 character identified in 33 of the 125 films. These characters were more likely to be portrayed as unintelligent (30%) than intelligent (2%), villains (15%) than heroes (4%), and followers (41%) than leaders (7%). Twenty-six (58%) were either mute or did not speak, 24% were portrayed as frightening, 35% had other physical deformities, and 35% were clumsy. Inter-rater reliability testing demonstrated substantial agreement (Kappa 0.61–0.80) or moderate agreement (κ 0.41–0.60) among raters for most of the character traits. CONCLUSIONS: Characters with strabismus are common in animated films, where they are significantly more likely to be portrayed negatively than positively. These films are marketed to children, and their negative depictions of strabismus likely exacerbate the social stigma faced by children with strabismus.

The Azerbaijani Adaptation of the Utrecht Work Engagement Scale: Investigating Its Influence on Psychological Distress and Life-Job Satisfaction

Teacher well-being plays a vital role in fostering a positive and productive learning environment, as it directly impacts both their professional performance and the overall success of their students. Consequently, this study aimed to adapt the Utrecht Work Engagement Scale for use among Azerbaijani school principals and to investigate the relationships between work and wellbeing, psychological distress, and life-job satisfaction. Data were gathered from 976 Azerbaijani school principals. The adaptation process involved confirmatory factor analysis, evaluations of criterion-related validity, and reliability testing. Additionally, the relationships between work and wellbeing, psychological distress, and life-job satisfaction were examined through mediational analysis. The confirmatory factor analysis revealed that the questionnaire consisted of 17 items, highlighted three factors, and demonstrated an acceptable model fit. The scale showed satisfactory reliability coefficients for both its individual factors and the overall score. Moreover, the results indicated that work and wellbeing was positively correlated with life-job satisfaction and negatively associated with psychological distress. Lastly, the findings revealed that psychological distress mediated the relationship between work and wellbeing and life-job satisfaction. These outcomes suggest that the Azerbaijani version of the Utrecht Work Engagement Scale possesses strong psychometric properties.

Adaptation of the Five-by-Five Resilience Scale in Indonesian

Resilience is the ability of individuals to be able to adapt and rise from difficult conditions or situations. The Five-by-Five Resilience Scale is a measurement tool developed by DeSimone et al (2016) to measure individual resilience. There are five protective factors of resilience, namely adaptation, emotional regulation, optimism, self-efficacy and social support. This research aims to adapt the Five-by-Five Resilience Scale into Indonesian. This research was conducted because there has been no testing of the Indonesian version of the Five-by-Five Resilience Scale. The subjects involved in the study were 267 subjects with an age range of 18 - 25 years who were domiciled in Indonesia. Based on the results of psychometric testing through Exploratory Factor Analysis (EFA), 4 factors were obtained that build the Five-by-Five Resilience Scale. The results also show that the Five-by-Five Resilience Scale has a correlation with other measuring tools in convergence validity testing. The reliability test results show that the dimensions on the Five-by-Five Resilience Scale as a whole have a reliability > 0.7.

Age-Related Differences and Reliability of a Field-Based Fitness Test Battery in Young Trained Footballers: The Role of Biological Age

Background: the purpose of this study was to analyze the reliability of a field-based fitness test battery in young trained football players, according to biological age. Methods: 197 young trained football players (12–19 years old) participated in the study. We measured anthropometric measurements (i.e., height, sitting height, length leg, and body mass), a bilateral vertical jumping test (CMJ), a progressive loading test of squats and hip thrust, acceleration and speed tests (10 m and 30 m sprint tests), a change-of-direction ability test (V–cut test), and a cardiorespiratory fitness test (30–15 intermittent fitness test). Statistical data are shown as the mean ± standard deviation by PHV group in tests and retests. Test reliability was assessed through the intraclass correlation coefficient (ICC), with an ICC above 0.9 being considered high. To evaluate accuracy and repeatability, standard error of measurement, coefficient of variation, and minimum detectable change at 90% were determined and Bland–Altman diagrams were used, establishing a statistical significance of p < 0.05. Results: All of the tests showed non-significant differences between the test and retest in the pooled sample (p > 0.05). Furthermore, all of them presented a trivial effect size (<0.2) and high intraclass correlation coefficients (>0.9), which indicates the high reproducibility of the tests, despite some of them presenting a significant difference between trials (i.e., the CMJ, 10 m sprint, V–cut, and squat tests). Low measurement errors were found for all tests (coefficient of variation [CV] = 4.39–9.39), except for the CMJ and the progressive loading test for squat and hip thrust exercises (CV = 12.2–21.11). Similar results were found irrespective of biological age group. Conclusions: All tests were reliable for the pooled sample as well as for the biological age groups.

Validity and reliability testing of the Shatri Sinulingga psychosomatic test (SSPT) questionnaire as a screening instrument for psychosomatic disorders in Indonesia

The incidence of psychosomatic disorders is increasing in Indonesia, and therefore screening instruments that are culturally appropriate for the Indonesian population are needed. The aim of this study was to assess the validity and reliability of the Shatri Sinulingga psychosomatic test (SSPT) questionnaire as a screening instrument for psychosomatic disorders in Indonesia. An analytic descriptive cross-sectional study divided into two stages (questionnaire formulation and distribution through the Psikosom.id application) was conducted in 2023. The validity test was carried out using the product moment technique, and Cronbach's alpha assessment was carried out to evaluate the reliability of the questionnaire. A total of 372 participants were included, with a mean age of 39.24 years old. The SSPT questionnaire scores had strong correlations with the scores of hospital anxiety and depression scale (HADS) (r=0.668; p<0.001) and the Symptom Checklist-90 (SCL-90) (r=0.674; p<0.001); and moderately correlated with the score of fatigue severity scale (FSS) (r=0.505; p<0.001) and Pittsburgh Sleep Quality Index (PSQI) (r=0.492; p<0.001). The correlation coefficient (r) value of each SSPT question ranged from 0.389–0.726, and all r higher than r table with an overall Cronbach’s alpha of 0.744. The SSPT questionnaire's strong predictive performance: had a sensitivity of 75.6% and specificity of 71.4% with an area under the curve (AUC) of 81% (95%CI: 76.6–85.4%; p<0.001) to HADS; sensitivity of 80% and specificity of 58.6% with an AUC of 75.1% (95%CI: 70–80.2%; p<0.001) to FSS; sensitivity of 64% and specificity of 67.5% with an AUC of 71.2% (95%CI: 65.4–77%; p<0.001) to PSQI; and had sensitivity of 78% and specificity of 67.4% with an AUC of 80.9% (95%CI: 76.6–85.3%; p<0.001) to SCL-90. The study highlights that the SSPT questionnaire is valid and reliable to be used as a screening instrument for psychosomatic disorders in Indonesia.

Influence of Temperature Variability on the Efficacy of Negative Ions in Removing Particulate Matter and Pollutants: An Experimental Database

Cities globally must make urgent decisions to ensure a sustainable future as rising pollution, particularly PM2.5, poses severe health risks like respiratory and heart diseases. PM2.5’s harmful composition also impacts vegetation and the environment. Immediate government intervention is necessary to mitigate these effects. This study tackles the urgent problem of reducing PM2.5 levels in Medellín’s urban and indoor environments, where pollution presents serious health risks. To explore effective solutions, this research provides new data on the interaction between particulate matter from various pollutants and negative ions under different temperature conditions, offering valuable insights into air quality improvement strategies. Using a high-voltage system, ions bind to pollutants, accelerating their removal. Experiments measured temperature, humidity, formaldehyde, volatile organic compounds, negative ions, and PM2.5 in a 40 cm3 chamber across various conditions. Pollutants tested included cigarette smoke, incense, charcoal, and gasoline at two voltage levels and three temperature ranges. The data, available in CSV format, were based on 36,000 samples and repeated tests for reliability. This resource is designed to support studies investigating particulate matter control in urban and indoor environments, as well as to improve our understanding of negative ion-based air purification processes. The data are publicly available and structured in formats compatible with leading data analysis platforms.

Hindi version of the lower extremity functional scale: a study of translation, validation and cross-cultural adaptation

BACKGROUND: Lower Extremity Function Scale (LEFS) serves as a patient-reported outcome measure, used to evaluate the functional status of lower extremity in different musculoskeletal conditions. It was originally developed in English language (E-LEFS) and numerous translations in different languages are available to measure its usability among different cultures. OBJECTIVES: To translate the English version of the Lower Extremity Function Scale (E-LEFS) into Hindi language and to evaluate its content validity, cross-cultural adaptation along with the concurrent validity and reliability. METHOD: Process of forward and backward translation was carried out by two bilingual translators and a physiotherapist after obtaining developer’s approval, for which the Beaton guidelines were used. For the content validation, Delphi method of was employed in which 12 field experts of more than 5 years of experience participated. Cultural adaptation procedures were then carried out with 12 patients, who reported in out-patient department of the hospital through convenience sampling who were asked about whether the H-LEFS was well understood by the patients. Their responses were recorded as positive and negative responses. This process was followed by concurrent validation which was conducted by comparing the scores with the SF-36 scale, involving 30 patients with activity limitation in their daily life. Lastly, 50 patients were recruited for intra-rater reliability testing. RESULT: The scale was successfully translated and verified by the bi-lingual language experts. Content validity came out to be significant with each item scoring more than 0.92. Nearly all items, excluding item no. 8, 16, 17, 18, and 19 received universal agreement with a content validity ratio of 1. The average scale-level validity stood at 0.98, indicating excellent level of validity and the scale was found to be well adapted by the native people as out of 12 patients, 10 patients provided 100% positive response. Concurrent validation using Pearson’s correlation coefficient came out excellent with the values of 0.993 for E-LEFS and 0.890 for SF-36. Cronbach's alpha and ICC values were calculated as 1.00 for Intra-rater reliability. CONCLUSION: LEFS was well translated into Hindi language with an excellent content and concurrent validity and is found to be highly reliable. Hence, it is recommended for the usage of native Hindi speaking population.

Causal relationship between leukocyte telomere length and two cardiomyopathies based on a bidirectional Mendelian randomization approach

This study aims to employ the Mendelian randomization (MR) approach to investigate the relationship between leukocyte telomere length (TL) and 2 prevalent forms of cardiomyopathies. Using R software (4.3.1) for MR study, independent genetic variants associated with leukocyte TL were extracted from the Integrative Epidemiology Unit database, while cardiomyopathies data were pooled from FinnGen and European Bioinformatics Institute databases. Analytical methodologies included inverse-variance weighting, MR-Egger regression, and weighted median methods. Further analyses involved MR-Egger intercept and MR-PRESSO for handling horizontal pleiotropy and Cochran Q test for study heterogeneity. Our forward Mendelian randomization study indicates a positive correlation between longer leukocyte TL and the risk of 2 forms of cardiomyopathies: the longer the leukocyte telomere, the higher is the risk of cardiomyopathies. Specifically, for hypertrophic obstructive cardiomyopathy the OR is 2.23 (95% CI: 1.19–4.14, P = .01), for hypertrophic cardiomyopathy the OR is 1.80 (95% CI: 1.14–2.85, P = .01), and for dilated cardiomyopathy the OR is 1.32 (95% CI: 1.01–1.71, P = .04). In contrast, our reverse Mendelian randomization showed that cardiomyopathies were not directly associated with TL, and the inverse-variance-weighted test was not statistically significant for any of the 3 (P > .05). The reliability tests for the forward Mendelian randomization, including both MR-Egger intercept and MR-PRESSO tests, show no evidence of horizontal pleiotropy, and Cochran Q test indicates no heterogeneity. The “leave-one-out” sensitivity analysis revealed no outlier genes. The reliability tests for the reverse Mendelian randomization, including both MR-Egger intercept and MR-PRESSO tests, also indicate no genetic pleiotropy. Despite the heterogeneity shown in our study between hypertrophic cardiomyopathy and leukocyte TL, the sensitivity analysis did not identify any anomalies. Our Mendelian randomization study suggests that longer leukocyte TL is associated with an increased risk of hypertrophic obstructive cardiomyopathy, hypertrophic cardiomyopathy, and dilated cardiomyopathy. However, the onset of these 2 kinds of disease does not directly lead to changes in leukocyte TL.

Invisible learning in the investigative competence of Lima schoolchildren

The present study, developed under a quantitative approach, explanatory scope and causal correlational design, aims to determine the influence of invisible learning on the research competence of high school students in two private schools in the city of Lima, Peru, whose educational models seek to develop autonomous learning and research through discovery learning and experimentation. Two questionnaires were applied to 120 students of the VII cycle of basic education, one to measure the perception regarding invisible learning with 20 items and the other to measure investigative competencies with 21 items; both instruments underwent the corresponding validity and reliability tests before their application. Among the main findings, descriptive results were obtained at a medium level for both variables, the correlations found were significant and moderate, and as for influence, the coefficient of determination R2 yielded a value of 0.13, suggesting that 13% of investigative competence is predicted by invisible learning. These results show that autonomy, the use of digital technologies, metacognition and other aspects that are part of invisible learning prepare students to solve problems of varying complexity, allowing them to face the challenges of contemporary knowledge in an innovative and effective manner.

Hafnium dioxide obtained by atomic layer deposition possesses the ability to induce the formation of biological apatite

The research explored the spontaneous formation of the BAp precursor on surfaces of HfO2 films obtained by Atomic Layer Deposition (ALD). It has been found that a careful selection of ALD growth conditions, followed by rapid thermal annealing (RTP) is crucial to achieve bioactivity of the films. Simulated Body Fluid experiment was used as a reliable test of the film's functionality as bone implant coating. SEM, XRD and XPS investigations proved that amorphous calcium orthophosphate structures were formed on the HfO2 films. These structures are of importance for biomineralization of bones. Physical and chemical characterization of the films was an integral part of the research. Such approach allowed us to achieve biomaterial functionality while maintaining the films’ quality. The finding has significance for future progress in personalized traumatological medicine.

Faktor yang Berhubungan dengan Perilaku Petani Padi dalam Penggunaan Pestisida di Desa Muara, Cilamaya Wetan Kabupaten Karawang

Unwise use of pesticides can cause residues that are harmful to human health and have a negative impact on the environment. Factors related to farmers' behavior can be one of the key determinants of how far they understand and implement safe, sustainable pesticide use practices. The study aims to analyze the factors associated with the behavior of peasants in Muara village, Cilamaya Wetan district, Karawang district. Behavior includes knowledge, attitudes, and actions consisting of aspects of pesticide type, dosage, time, spray frequency, self-protection, pesticides application techniques, and compliance with instructions for use. The research was conducted from September to October 2023. Sampling proportionate stratified random sampling obtained 85 respondents. Data collection techniques include observations, tests, questionnaires, and interviews. The study used a survey method using Spearman Rank analysis. Data were analyzed using validity tests, reliability tests, quantitative descriptive analysis, and Spearman Rank correlation tests. The results of the research showed that the factors associated with the behavior of wildfish farmers in the use of pesticides were land area (X3) with a correlation of 0.239, farming experience (X4) with the correlations of 0.258, and perception of pests and plant diseases (X7) with a correlation of 0,233. Whereas education (X1), income (X2), social involvement (X5), as well as the role of the farmer (X6) had no significant correlation with farmers' behaviour in pesticide use.

Covid-19 pandemic and its economic and psychological impacts on Saudi workers: An empirical study in the kingdom of Saudi Arabia

Background: The COVID-19 pandemic has had a substantial economic and psychological impact on workers in Saudi Arabia. The objective of the study was to assess the effects of the COVID-19 epidemic on the financial and mental well-being of Saudi employees in the Kingdom of Saudi Arabia. Purpose: The COVID-19 epidemic has resulted in significant economic and societal ramifications. Current study indicates that the pandemic has not only precipitated an economic crisis but has also given rise to several psychological and emotional crises. This article provides a conceptual examination of how the pandemic impacts the economic and mental health conditions of Saudi workers, based on contemporary Structural Equation Modeling (SEM) models. Method: The current study employed a qualitative methodology and utilized a sample survey strategy. The data was gathered from Saudi workers residing in major cities of Saudi Arabia. The samples were obtained from professionals such as managers, doctors, and engineers, as well as non-professionals like unskilled and low-skilled laborers, who are employed in various public and private sectors. A range of statistical tools, including Descriptive statistics, ANOVA, Pearson’s Correlation, Factor analysis, Reliability test, Chi-square test, and regression approach, were employed to analyze and interpret the results. Result: According to the data, the pandemic has caused a wide range of economic problems, including high unemployment and underemployment rates, income instability, and different degrees of pressure on workers to find work. Feelings of insecurity (about food and environmental safety), worry, dread, stress, anxiety, depression, and other mental health concerns have been generated by these challenges. The rate of mental health decline differs among demographics. Conclusions: The COVID-19 pandemic has universally affected all aspects of our lives worldwide. It resulted in an extended shutdown of educational institutions, factories, offices, and businesses. Without a question, it has profoundly transformed the work environment, professions, and lifestyles of billions of individuals worldwide. There is a high occurrence of poor psychological well-being among Saudi workers. However, it has been demonstrated that both economic health and mental health interventions can effectively alleviate the mental health burden in this population.

Interdisciplinary Operating Room Ergonomics Needs and Priorities: A Survey of Operating Room Staff.

To examine perceived OR ergonomics facilitators and barriers, with a focus on the interdisciplinary team. Poor ergonomics causes musculoskeletal injuries affecting all operating room (OR) staff with repercussions on patient care, outcomes, and sustainability. Lack of ergonomic awareness and education are risk factors. We conducted a self-administered web-based survey of OR nurses, surgeons, and anesthesiologists at a single centre (n=238). We developed a questionnaire through items generation and reduction, followed by reliability and validity testing. Response rate was 53.8%. Respondents perceived that on average 80% of nurses, 70% of surgeons, and 40% anesthesiologists experienced MSK injuries, with no difference in professional groups' perceptions. Guideline ergonomics interventions were rarely used (<25%) except for specialized clothing (33%), equipment repositioning (59%), and seating (37%), though perceived beneficial by 80-90%. Reported barriers to optimal ergonomics were organizational/structural (lack of time, space, equipment, funding), whereas solutions were individual. Fear of unfavourable perception from others was a concern for 62%. Teams discussing, prioritizing, monitoring, or helping with ergonomics was indicated by <50%. Individual ergonomic adaptations were perceived as convenience by other staff. While structural/organizational issues are reported as barriers to ergonomics, solutions appeared as individual responsibilities. Team dynamics did not prioritize nor support ergonomics. Education tools leveraging the interdisciplinary team are warranted. This work will be supplemented by interviews and live observations to build tailored educational tools for OR teams.

Study of High-Frequency Pulsating Flow Control Valves

Pulsating flow signals are widely used in the fields of dynamic calibration of flow meters and reliability testing of fluid transmission pipelines. A high-frequency pulsating flow control valve is proposed, its structure and working principle are introduced, and its mathematical model is also established. Finally, an experimental platform is built to experimentally verify the performance of the high-frequency pulsating flow signal generation of this flow control valve prototype. When applying sinusoidal flow signals of 1–25 Hz, the amplitude of the output flow starts to decay after 2 Hz, and the frequency corresponding to the decay to −3 dB is about 23.5 Hz. The experimental results show that the valve can effectively generate unidirectional sinusoidal flow with a maximum amplitude of 25 L/min and a frequency of 23.5 Hz.

Review of the Hazards and Contraindications of Etomidate.

Etomidate, an ultrashort-acting non-barbiturate sedative derived from imidazole, exerts potent inhibitory effects on the central nervous system. It is commonly employed for the induction of intravenous general anaesthesia or assisted anaesthesia. Recently, etomidate has emerged as an alternative to narcotics and novel psychoactive substances, leading to an increasing trend of abuse. Chronic overdose of etomidate can result in irreversible brain damage and various mental disorders. Severe cases may manifest as mental disturbances, behavioural disorders, self-mutilation and even death. The toxicological mechanisms of etomidate remain poorly understood. Additionally, there is limited information on the clinical symptoms and histopathological changes associated with etomidate poisoning and standardized detection methods for etomidate in blood, urine and hair are lacking. Consequently, further research on toxicological pathology and the development of reliable testing methods is crucial. This study reviews the metabolism, distribution, adverse reactions, poisoning manifestations, toxicology mechanisms and testing methods of etomidate.

Aviation business growth; Does ground movement capacity and air space capacity matter?

Growth in any firm’s business is critical as a long-term objective of such a firm. The study thus focused on the influence of ground movement capacity and air space capacity on the growth of aviation businesses at Wilson Airport. The guiding objectives of this study were: to establish the effect of ground movement capacity on aviation business growth in Kenya and to assess the effects of airspace capacity on in Kenya. The study was guided by theory of constraints. The study employed an explanatory research design. The study targeted 117 respondents based at Wilson Airport. A Census survey was used in this study to collect information from all participants in the population. The study collected primary data using questionnaires. In testing for reliability, Cronbach’s alpha coefficient was applied. The Statistical Package for the Social Sciences (SPSS) was used for data analysis. Inferential data were analyzed using correlation and multiple regressions. The study findings revealed that ground movement capacity has a positive and significant effect on aviation business and airspace capacity has a positive and significant effect on aviation business growth. Ground movement capacity was an important determinant of aviation business growth. Kenya Civil Aviation Authority and Kenya Airports Authority should pool resources and increase the ground movement equipment and facilities. The study recommends that future researcher’s study on the influence of public, private partnership to increase the ground movement capacity on aviation growth can also be conducted.

Pengaruh Promosi Dan Harga Terhadap Keputusan Pembelian Honda Beat Pada PT Tunas Honda Natar

This research aims to determine the influence of promotions and prices on purchasing decisions for Honda Beat at PT Tunas Honda Natar. The data used in this research is primary data, namely data collected by the researcher himself directly from the first source or place where the research object was carried out, namely the results of interviews and questionnaires distributed to consumers at PT Tunas Honda Natar. As well as secondary data, namely data collected from other parties, namely books, articles and journals related to this research. The analysis techniques used are validity and reliability tests, classical assumption tests, hypothesis tests, coefficient of determination (R2), and multiple linear regression tests. Based on the results of the analysis, it can be concluded that partially Promotion and Price have a significant effect on Purchasing Decisions and simultaneously Promotion and Price have a significant effect on Purchasing Decisions.

The relationship between pulmonary ventilation function and bone marrow hematopoietic function: A mendelian randomization analysis

Background: In recent years, the comorbidity between respiratory and hematopoietic system diseases has emerged as a new challenge. However, the potential genetic link between the ventilatory function of the respiratory system and the hematopoietic function of the bone marrow remains unclear. In this study, we conducted a comprehensive investigation into the possible genetic connection between lung ventilatory function and bone marrow hematopoietic function. Methods: We selected two exposure factors, Forced Expiratory Volume in 1 second (FEV1) and Forced Vital Capacity (FVC), which represent pulmonary ventilation function, and two outcome indicators, Immature Fraction of Reticulocytes (IFR) and Reticulocyte Count (RC), which represent bone marrow hematopoiesis function, from published genome-wide association studies. Based on the three core assumptions of Mendelian randomization analysis, we extracted Single Nucleotide Polymorphism (SNPs) associated with FEV1 and FVC as instrumental variables for the exposure factors. We then conducted two-sample Mendelian randomization analyses using inverse variance weighted (IVW), weighted median, and MR-Egger regression methods. Lastly, we assessed the reliability of the testing results through MR-Egger, Cochran’s Q test, and the leave-one-out test. Through these steps, we aimed to explore the causal relationship between pulmonary ventilation function and the outcome of bone marrow hematopoiesis function and evaluated the reliability of the testing results using methods such as MR-Egger, Cochran’s Q test, and the leave-one-out test. Results: The IVW method revealed that a decrease in FEV1 is associated with an increase in IFR (β = −0.072, 95% CI: −0.131 to −0.014, p = 0.01), and the results from MR-Egger regression showed a similar association (β = −0.169, 95% CI: −0.342 to −0.004, p = 0.05). Furthermore, a decrease in FEV1 is associated with an increase in RC (β = −0.143, 95% CI: −0.198 to −0.087, p =4.00E-07), and MR-Egger regression yielded consistent results (β = −0.216, 95% CI: −0.381 to −0.541, p=1.07E-02). Similarly, a decrease in FVC is associated with an increase in IFR (β = −0.073, 95% CI: −0.116 to −0.031, p = 6.17E-04), and MR-Egger regression showed a similar trend (β = −0.046, 95% CI: −0.160 to −0.067, p=0.42). Additionally, a decrease in FVC is associated with an increase in RC (β = −0.173, 95% CI: −0.221 to −0.125, p = 2.33E-12), and MR-Egger regression yielded consistent results (β = −0.142, 95% CI: −0.272 to −0.012, p = 3.32E-02). The reliability tests indicated heterogeneity in the above MR analyses but no evidence of horizontal pleiotropy. Therefore, a fixed-effect model IVW was used to explore the causal relationships, which were found to be robust and reliable with no outliers or significant bias in this study. Conclusion: This study indicates that there is a negative causal relationship between pulmonary ventilation function and bone marrow hematopoietic function. A decrease in pulmonary ventilation function stimulates bone marrow hematopoiesis. However, further research is needed to elucidate this mechanism.

Social media and amblyopia: evaluating content and intent on Instagram

ABSTRACT Introduction: Social media is a widely-used avenue for the public to find health information. In particular, Instagram is a popular social media platform among adults who may have children within the amblyogenic age range, and it may be used to find amblyopia-related information. The purpose of this study is to assess the quality of the amblyopia information on Instagram. Methods: The top 200 publicly available Instagram posts about amblyopia from October to December 2022 were reviewed and scored using the Currency, Reliability, Authority, and Purpose (CRAP) test. Results: A quarter of posts were from healthcare providers (ophthalmologists, optometrists, opticians), and only 3% of posts were specifically from pediatric ophthalmologists. Most posts, however, were from businesses (51%), which were of lower quality than posts by providers. Discussion: Instagram represents a tremendous opportunity to educate the public. Better utilizing this resource may allow medical professionals to broadly spread accurate, unbiased data.

Fosfomycin—Overcoming Problematic In Vitro Susceptibility Testing and Tricky Result Interpretation: Comparison of Three Fosfomycin Susceptibility Testing Methods

Background: Fosfomycin (FOS) is an older antimicrobial agent newly rediscovered as a possible treatment for infections with limited therapeutic options (e.g., Gram-negative bacteria with difficult-to-treat resistance, DTR), especially in intravenous form. However, for correct usage of FOS, it is necessary to have a reliable susceptibility testing method suitable for routine practice and robust interpretation criteria. Results: The results were interpreted according to 2023 interpretation criteria provided by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). DTR Gram-negatives were more likely to be resistant to FOS (45% in Enterobacterales and 20% in P. aeruginosa) than non-DTR (10% and 6.7%, resp.). All isolates of S. aureus were susceptible to FOS. In Gram-negatives, all agreement values were unacceptable. Etest® performed better in the DTR cohort (categorical agreement, CA, 80%) than in the non-DTR cohort (CA 45.7%). There were no very major errors (VREs) observed in P. aeruginosa. S. aureus had surprisingly low essential agreement (EA) rates (53% for MRSA and 47% for MSSA) for Etest®, but categorical agreement was 100%. Methods: A total of 130 bacterial isolates were tested and compared using the disc diffusion method (DD) and gradient strip method (Etest®) with the reference method (agar dilution, AD). The spectrum of isolates tested was as follows: 40 Enterobacterales (20 DTR vs. 20 non-DTR), 30 Pseudomonas aeruginosa (15 DTR vs. 15 non-DTR), and 60 Staphylococcus aureus (30 methicillin-susceptible, MSSA, vs. 30 methicillin-resistant, MRSA). Conclusions: Neither one of the tested methods was identified as a suitable alternative to AD. It would be beneficial to define more interpretation criteria, at least in some instances.