Interference is a significant issue in UART communication, often causing data jitter and distortion, particularly in industrial environments. This study addresses these challenges by developing an anti-interference UART system that includes the design and implementation of receiving, processing, and sending modules. The system utilizes techniques such as multi-level sampling, synchronization, and cumulative decision-making to enhance data transmission accuracy and reliability. In embedded systems, electromagnetic interference (EMI) poses a major problem, leading to potential data loss and communication failures. Reliable communication is crucial for maintaining the performance and safety of industrial operations. The anti-interference UART system developed in this study aims to improve operational stability by mitigating the effects of EMI. The system’s performance was validated through extensive simulations, demonstrating its ability to maintain stable and accurate data transmission even under various interference conditions. The results show significant improvements in data integrity and communication reliability. This paper presents a comprehensive approach to designing, implementing, and validating a robust UART system that can effectively operate in challenging environments, ensuring reliable data communication and enhancing overall system performance.