Relevant Locations Research Articles

Robustness of the microtubule network self-organization in epithelia.

Robustness of biological systems is crucial for their survival, however, for many systems its origin is an open question. Here, we analyze one subcellular level system, the microtubule cytoskeleton. Microtubules self-organize into a network, along which cellular components are delivered to their biologically relevant locations. While the dynamics of individual microtubules is sensitive to the organism's environment and genetics, a similar sensitivity of the overall network would result in pathologies. Our large-scale stochastic simulations show that the self-organization of microtubule networks is robust in a wide parameter range in individual cells. We confirm this robustness in vivo on the tissue-scale using genetic manipulations of Drosophila epithelial cells. Finally, our minimal mathematical model shows that the origin of robustness is the separation of time-scales in microtubule dynamics rates. Altogether, we demonstrate that the tissue-scale self-organization of a microtubule network depends only on cell geometry and the distribution of the microtubule minus-ends.

Metropolitan age-specific mortality trends at borough and neighborhood level: The case of Mexico City.

Understanding the spatial and temporal patterns of mortality rates in a highly heterogeneous metropolis, is a matter of public policy interest. In this context, there is no, to the best of our knowledge, previous studies that correlate both spatio-temporal and age-specific mortality rates in Mexico City. Spatio-temporal Kriging modeling was used over five age-specific mortality rates (from the years 2000 to 2016 in Mexico City), to gain both spatial (borough and neighborhood) and temporal (year and trimester) data level description. Mortality age-specific patterns have been modeled using multilevel modeling for longitudinal data. Posterior tests were carried out to compare mortality averages between geo-spatial locations. Mortality correlation extends in all study groups for as long as 12 years and as far as 13.27 km. The highest mortality rate takes place in the Cuauhtémoc borough, the commercial, touristic and cultural core downtown of Mexico City. On the contrary, Tlalpan borough is the one with the lowest mortality rates in all the study groups. Post-productive mortality is the first age-specific cause of death, followed by infant, productive, pre-school and scholar groups. The combinations of spatio-temporal Kriging estimation and time-evolution linear mixed-effect models, allowed us to unveil relevant time and location trends that may be useful for public policy planning in Mexico City.

The Critical Role of Head Movements for Spatial Representation During Bumblebees Learning Flight.

Bumblebees perform complex flight maneuvers around the barely visible entrance of their nest upon their first departures. During these flights bees learn visual information about the surroundings, possibly including its spatial layout. They rely on this information to return home. Depth information can be derived from the apparent motion of the scenery on the bees' retina. This motion is shaped by the animal's flight and orientation: Bees employ a saccadic flight and gaze strategy, where rapid turns of the head (saccades) alternate with flight segments of apparently constant gaze direction (intersaccades). When during intersaccades the gaze direction is kept relatively constant, the apparent motion contains information about the distance of the animal to environmental objects, and thus, in an egocentric reference frame. Alternatively, when the gaze direction rotates around a fixed point in space, the animal perceives the depth structure relative to this pivot point, i.e., in an allocentric reference frame. If the pivot point is at the nest-hole, the information is nest-centric. Here, we investigate in which reference frames bumblebees perceive depth information during their learning flights. By precisely tracking the head orientation, we found that half of the time, the head appears to pivot actively. However, only few of the corresponding pivot points are close to the nest entrance. Our results indicate that bumblebees perceive visual information in several reference frames when they learn about the surroundings of a behaviorally relevant location.

Range resolution enhancement of WISDOM/ExoMars radar soundings by the Bandwidth Extrapolation technique: Validation and application to field campaign measurements

The ExoMars 2022 rover mission has been designed to search for traces of life, past or present, on Mars and more specifically below its hostile surface. This will be the first mission able to collect samples down to 2 ​m in the Martian subsurface, where organic molecules and bio-signatures may be preserved. The images of the subsurface (or radargrams) provided by the ground penetrating radar WISDOM onboard the ExoMars rover will be key to select the safest and scientifically most relevant locations for drilling. However, the vertical resolution of WISDOM radargrams is limited (to ~5.5 ​cm in a typical soil of dielectric constant 4) by the bandwidth of the instrument when applying classical processing techniques. Here we propose to enhance WISDOM radargrams vertical resolution by implementing the Bandwidth Extrapolation (BWE) technique. We demonstrate that this technique yields an improvement of WISDOM vertical resolution by a factor of 3 while well preserving the time of arrival and amplitude of the detected echoes. We validate the BWE technique on synthetic data before applying it to experimental observations acquired both in controlled and natural environments. The resulting super-resolved radargrams greatly improve our understanding of the investigated terrains with a better separation of echoes from underground reflectors, and demonstrate the value of implementing the BWE technique in the WISDOM data processing pipeline which will ultimately provide images of the Martian subsurface down to a few meters depth with a vertical resolution of ~2 ​cm in typical soils.

Definition and use of functional analogues in planetary exploration

The practical limitations inherent to human and robotic planetary exploration necessitate the development of specific protocols and methods. This non-standard approach requires testing and validation phases in order to optimise instrumental setups and improve data interpretation; this can occur prior to, during, or even after a mission. Flight instruments, and/or their spare models, may be evaluated using relevant terrestrial materials and/or locations. These materials are called analogues: “analogue sites” for large-scale locations, and “analogue samples” for smaller-scale materials. Depending on the scientific domain, the word “analogue” may carry different meanings but is invariably used to denote objects having compositions and/or physical properties similar to specific extraterrestrial objects. However, due to the variability in composition and properties of natural materials, there are always – inevitably – some differences between the analogue and the object(s) to which it refers. In analogue studies, it is, thus, important to focus on the specific properties that need to be imitated and to consider analogue properties rather than analogue sites or samples alone. Here, we introduce the concept of “functional analogues”. We first make an overview of the different types of analogues and sort them according to their utility. We then describe how different types of functional analogues can be used throughout the timeline of space missions, from the evaluation of different methods in the definition of a mission to understanding the results acquired by probes exploring extraterrestrial bodies. Finally, logical pathways are outlined that facilitate the selection of the best-suited functional analogue(s) according to their intended use and taking into account practical limitations.

Extracting and modeling geographic information from scientific articles.

Scientific articles often contain relevant geographic information such as where field work was performed or where patients were treated. Most often, this information appears in the full-text article contents as a description in natural language including place names, with no accompanying machine-readable geographic metadata. Automatically extracting this geographic information could help conduct meta-analyses, find geographical research gaps, and retrieve articles using spatial search criteria. Research on this problem is still in its infancy, with many works manually processing corpora for locations and few cross-domain studies. In this paper, we develop a fully automatic pipeline to extract and represent relevant locations from scientific articles, applying it to two varied corpora. We obtain good performance, with full pipeline precision of 0.84 for an environmental corpus, and 0.78 for a biomedical corpus. Our results can be visualized as simple global maps, allowing human annotators to both explore corpus patterns in space and triage results for downstream analysis. Future work should not only focus on improving individual pipeline components, but also be informed by user needs derived from the potential spatial analysis and exploration of such corpora.

Heuristic methods for minimal controller location set problem in transportation networks

To be used efficiently, transportation networks require control strategies to optimize flow distribution. However, the location and type of controllers employed in a transportation network directly impact the level of performance reachable by traffic control policies. In order to guarantee that the highest level of performance is reachable, fully exploiting the available network capacity and reducing negative externalities, we explore methods to identify relevant controller locations in transportation networks while minimizing the number of controllers used. Previous work provided an exact approach to identify controller locations on complex networks. However, said approach exhibits complications when applied to transportation networks containing bi-directional links. We therefore propose simple heuristic algorithms relying only on topological information to solve this problem, while also avoiding heavy computation, thus being able to determine a solution to the minimal controller location set problem on large networks. Based on the existing framework, we aim to provide an experimental setup, with diverse network sizes and configurations to analyze the performances of different heuristic methods, in order to develop an efficient algorithm.

An analysis of open data as a location factor

Research background: The publication of data in Germany introduces a paradigm shift in public administrations. The data silos of the administrations are thus being opened up piece by piece. There are many possible applications for these data. Optimized location determination for trade, production, and services is one of these many possible applications. It has been shown that the provision of open data can achieve economic effects and set value chains in motion. The value creation effects can arise directly, indirectly, or downstream. Public data can thus become a relevant location factor for regions and cities. Purpose of the article: The various implications of opening up data in context with location determination will be examined in this article. This will be done from an economic perspective, highlighting the positive externalities of open data. Methods: The published data of administrations will be analyzed based on a German data bank of 12,020 datasets concerning their relevance for determining location. For this purpose, hard and soft location criteria will be defined in advance. Findings & Value added: The result of the study will be a newly developed publication strategy for German public administrations on location criteria. With the help of this publication strategy, open data should function as a location advantage and initiate value creation effects.

Zenithal Sky Brightness Measurement at Selangor Observatory

The first continuous assessment of night sky brightness (NSB) at Selangor Observatory are presented. The brightness at night sky was monitored over 6 months in 2018, using portable light sensor called the Sky Quality Meter-type USB-Data Logging with a fixed position - pointing towards zenith. From the 179 records of night, the study shows that the average NSB readings at Selangor Observatory is 20.04 ± 0.18 magnitude per square arc second (MPSAS), after excluding the moonlight factor. Meanwhile, during the stable night sky conditions, the most recorded NSB value is 21.1 MPSAS with 0.78 MPSAS range and 21.03 MPSAS in average. Therefore, Selangor Observatory is still a relevant location for the implementation of astronomical work since it has a night sky within Bortle scale Class 4 to 5.

Dialect syntax in Construction Grammar

Abstract This squib focuses on two main issues. Firstly, it examines the ways in which constructionist approaches to language can bring about an improved theoretical understanding of Double Modals (DMs) in dialects of English. DMs have proved to be a long-lasting, notorious puzzle in formal linguistics, and have not received any general solution today, with much analysis devoted to their constituent structure and their postulated layers of derivation, especially in generative models of language. Usage-based strands of Construction Grammar (CxG) appear to naturally overcome such problems, while conveying a more cognitively and socially realistic picture of such dialect variants. Secondly, and more importantly, we argue that such an improved, constructional understanding of DMs can also contribute to advances in the modeling of dialect syntax in CxG, both theoretically and methodologically. In particular, DMs constitute an interesting case of relatively rare and restricted syntactic constructions in the dialects they appear in, and they are likely to exhibit different rates of entrenchment and network schematicity cross-dialectally. Moreover, the empirical challenges surrounding the measurement of DM usage invite us to refine the methodological concept of triangulation, by sketching a two-step approach with a data-driven study of new types of corpora on the one hand, and a hypothesis-driven experimental account of acceptability in relevant geographical locations on the other.

Focality of the Induced E-Field Is a Contributing Factor in the Choice of TMS Parameters: Evidence from a 3D Computational Model of the Human Brain.

Transcranial magnetic stimulation (TMS) is a promising, non-invasive approach in the diagnosis and treatment of several neurological conditions. However, the specific results in the cortex of the magnitude and spatial distribution of the secondary electrical field (E-field) resulting from TMS at different stimulation sites/orientations and varied TMS parameters are not clearly understood. The objective of this study is to identify the impact of TMS stimulation site and coil orientation on the induced E-field, including spatial distribution and the volume of activation in the cortex across brain areas, and hence demonstrate the need for customized optimization, using a three-dimensional finite element model (FEM). A considerable difference was noted in E-field values and distribution at different brain areas. We observed that the volume of activated cortex varied from 3000 to 7000 mm3 between the selected nine clinically relevant coil locations. Coil orientation also changed the induced E-field by a maximum of 10%, and we noted the least optimal values at the standard coil orientation pointing to the nose. The volume of gray matter activated varied by 10% on average between stimulation sites in homologous brain areas in the two hemispheres of the brain. This FEM simulation model clearly demonstrates the importance of TMS parameters for optimal results in clinically relevant brain areas. The results show that TMS parameters cannot be interchangeably used between individuals, hemispheres, and brain areas. The focality of the TMS induced E-field along with its optimal magnitude should be considered as critical TMS parameters that should be individually optimized.

Orthotopic Implants in Mice.

By directly implanting patient tumor cells into mice in a relevant location, we can mimic both the biology and tumor microenvironment of the original tumor. Here we describe the process of generating an orthotopic patient derived xenograft model by injecting a single cell suspension of Ewing sarcoma cells into the femur of a recipient mouse.

Production of pulsed high-energy neutron bursts from beam–target interaction using a 15 MeV HERMES III ion beam

Intense pulsed neutron fluences are generated by a high-energy (>10 MeV) proton beam using the beam-target method on the HERMES III facility at Sandia National Laboratories [J. J. Ramirez et al., in Proceedings of the 7th International Conference on High Power Particle Beams (Kernforschungszentrum, Karlsruhe GmbH, Karlsruhe, Germany, 1988), p. 148]. In order to generate the high-energy proton beam, a radial ion diode previously developed and fielded at the 6-MeV level in negative polarity was extended in performance to the 15-MeV level. This performance increase is described along with the development of a more durable hardware set to withstand the much more potent 15-MeV proton beam. An extensive series of simulations is developed to characterize the neutrons produced by the proton–target interaction. Particle-in-cell simulations describe the electron and ion dynamics, while Monte Carlo simulations characterize the neutron output. Due to differing estimates of proton beam voltage and current between the respective simulations, we make an approximate estimate of 13.5-MeV to 15-MeV and 120-kA ion beams at peak power in a 40 ns FWHM pulse. Simulations indicate that a total of 1.7 × 1013 neutrons are generated into 4π. Comparison of the neutron output predictions with a limited set of neutron flux measurements suggests a flux level of ∼1 × 1010 neutrons/cm2 to 10 × 1010 neutrons/cm2 over an approximately few tens of cm2 area at the relevant application location. This effort also contributes to physics understanding of the use of inductive voltage adder platforms to drive ion-beam diode loads.

Sustained spatial attention accounts for the direction bias of human microsaccades

Small ballistic eye movements, so called microsaccades, occur even while foveating an object. Previous studies using covert attention tasks have shown that shortly after a symbolic spatial cue, specifying a behaviorally relevant location, microsaccades tend to be directed toward the cued location. This suggests that microsaccades can serve as an index for the covert orientation of spatial attention. However, this hypothesis faces two major challenges: First, effects associated with visual spatial attention are hard to distinguish from those that associated with the contemplation of foveating a peripheral stimulus. Second, it is less clear whether endogenously sustained attention alone can bias microsaccade directions without a spatial cue on each trial. To address the first issue, we investigated the direction of microsaccades in human subjects while they attended to a behaviorally relevant location and prepared a response eye movement either toward or away from this location. We find that directions of microsaccades are biased toward the attended location rather than towards the saccade target. To tackle the second issue, we verbally indicated the location to attend before the start of each block of trials, to exclude potential visual cue-specific effects on microsaccades. Our results indicate that sustained spatial attention alone reliably produces the microsaccade direction effect. Overall, our findings demonstrate that sustained spatial attention alone, even in the absence of saccade planning or a spatial cue, is sufficient to explain the direction bias observed in microsaccades.

Mapping the Progressive Treatment-Related Reduction of Active MRI Lesions in Multiple Sclerosis.

Objective: To assess treatment-related spatio-temporal dynamics of active MRI lesions in relapsing-remitting multiple sclerosis (RRMS) patients.Methods: We performed a post-hoc analysis of MRI data acquired at weeks 4, 8, 12, and 16, in RRMS patients from the multicenter randomized IMPROVE study, which compares patients treated with 44 mcg subcutaneous interferon β-1a three times weekly (n = 120) versus placebo (n = 60). We created lesion probability maps (LPMs) of the cumulative combined unique active (CUA) lesions in each patient group at each time point. Group differences were tested in terms of lesion spatial distribution and frequency of occurrence.Results: Spatial distribution of CUA lesions throughout the study was less widespread in the treated than placebo group, with a 50% lower lesion accrual (24 vs. 48 cm3/month). Similar results were obtained with the WM tract analysis, with a reduction ranging from −47 to −66% in the treated group (p < 0.001). On voxel-wise analysis, CUA lesion frequency was lower in the treated group than the placebo group at week 4 (p = 0.07, corrected), becoming particularly pronounced (p ≤ 0.03, corrected) from week 8 onwards in large clusters of WM tracts, with peaks along fronto-parietal parts of the corticospinal tract, thalamic radiation, and superior longitudinal fascicle.Conclusion: LPM showed, in the short term, a treatment-related reduction of MRI lesion activity in RRMS patients in specific, clinically relevant brain locations. Such a quantitative approach might be a promising additional endpoint in future MS studies alongside the number and volume of WM lesions.Clinical Trial Registration: ClinicalTrials.gov identifier NCT00441103.

The effect of Toll-like receptor stimulation on the motility of regulatory T cells

Regulatory T cells (Tregs) have suppressive functions and play an important role in controlling inflammation and autoimmunity. The migratory capacity of Tregs determines their location and their location determines whether they inhibit the priming of naïve lymphocytes in lymphoid tissues or the effector phase of immune responses at inflamed sites. Tregs generated or expanded in vitro are currently being tested in clinics for the treatment of autoimmune disorders, however, little is known about the factors controlling their migration towards therapeutically relevant locations. In this study, we have modulated Treg dynamics using Toll-like receptor (TLR) agonists. Dynamic imaging with confocal and two-photon microscopy revealed that Tregs generated in vitro and stimulated with P3C (a TLR2 agonist) but not with R848 (a TLR7 agonist) or LPS (a TLR4 agonist) showed enhanced cell migration within splenic white pulp or draining lymph node when transferred into mice intravenously or into the footpad, respectively. In summary, our data demonstrate that Tregs are more motile in response to direct TLR stimulation in particular towards TLR2 signals. This may have implications for efficient clinical Treg induction protocols.

Targeted Activation of Hippocampal Place Cells Drives Memory-Guided Spatial Behavior

SummaryThe hippocampus is crucial for spatial navigation and episodic memory formation. Hippocampal place cells exhibit spatially selective activity within an environment and have been proposed to form the neural basis of a cognitive map of space that supports these mnemonic functions. However, the direct influence of place cell activity on spatial navigation behavior has not yet been demonstrated. Using an ‘all-optical’ combination of simultaneous two-photon calcium imaging and two-photon optogenetics, we identified and selectively activated place cells that encoded behaviorally relevant locations in a virtual reality environment. Targeted stimulation of a small number of place cells was sufficient to bias the behavior of animals during a spatial memory task, providing causal evidence that hippocampal place cells actively support spatial navigation and memory.

Review of Post-Licence Motorcycle Rider Training in New South Wales

Fully licensed motorcyclists represented over two thirds of riders killed on New South Wales (NSW) roads from 2010 – 2014. An ongoing need to address crash risks among this cohort is recognised and there is strong support for post-licence rider training (PLRT) among rider advocates and stakeholders. This research, commissioned by Transport for NSW, examined the PLRT environment in NSW to assess the extent to which courses targeted specific rider skills and competencies. Before commencement of this research, key riding competencies were identified by Transport for NSW in consultation with motorcycle stakeholder groups, and included scanning, buffering, setting up brakes, basic motorcycle handling, cornering, and lane positioning. A desktop review of 40 available courses provided an overview of relevant course content, locations and costs. The review was supplemented by interviews with eight training providers to gather information on course structure, components, delivery, promotion and trainee characteristics. The collective information was used to identify which training options support riders’ risk management and promote improved safety outcomes. A wide range of courses was identified, and in most cases there was no standardised curriculum. Most courses appeared to support rider risk management and most also appeared to address roadcraft and defensive riding principles, albeit to varying degrees. Providers noted participant diversity in characteristics, needs and motivations for undertaking rider training, reflecting a need for a diverse range of course offerings including individualised training. Key groups were catered for including returning riders, female riders, inexperienced riders, commercial riders and aspirational racers.

On-to-off-path gaze shift cancellations lead to gaze concentration in cognitively loaded car drivers: A simulator study exploring gaze patterns in relation to a cognitive task and the traffic environment

Appropriate visual behaviour is necessary for safe driving. Many previous studies have found that when performing non-visual cognitive tasks, drivers typically display an increased amount of on-path glances, along with a deteriorated visual scanning pattern towards potential hazards at locations outside their future travel path (off-path locations). This is often referred to as a gaze concentration effect. However, what has not been explored is more precisely how and when gaze concentration arises in relation to the cognitive task, and to what extent the timing of glances towards traffic-situation relevant off-path locations is affected. To investigate these specific topics, a driving simulator study was carried out. Car drivers’ visual behaviour during execution of a cognitive task (n-back) was studied during two traffic scenarios; one when driving through an intersection and one when passing a hidden exit. Aside from the expected gaze concentration effect, several novel findings that may explain this effect were observed. It was found that gaze shifts from an on-path to an off-path location were inhibited during increased cognitive load. However, gaze shifts in the other direction, that is, from an off-path to an on-path location, remained unaffected. This resulted in on-path glances increasing in duration, while off-path glances decreased in number. Furthermore, the inhibited off-path glances were typically not compensated for later. That is, off-path glances were cancelled, not delayed. This was the case both in relation to the cognitive task (near-term) and the traffic environment (far-term). There was thus a general reduction in the number of glances towards situationally relevant off-path locations, but the timing of the remaining glances was unaffected. These findings provide a deeper understanding of the mechanism behind gaze concentration and can contribute to both understanding and prediction of safety relevant effects of cognitive load in car drivers.

All for one and one for all? Integration in high-performance sport

ABSTRACT Purpose : To examine the lived experiences of the integrated model of elite sport from the perspective of parasport insiders. Research design : Since 2002, an integrated model for competition has been implemented by Commonwealth Games Federation (CGF). Commonwealth Games XXI was selected as a relevant location to conduct research regarding integration. Ethnographic methods included interviews, go-along conversations and observation. Interviews were conducted with Games personnel to enhance understandings of integration in sport. Qualitative thematic analysis was conducted to determine significant themes. Findings : The findings confirmed that integration is considered the preferred model among non-athletes who were unanimous in their support. Analysis indicated that the parasport athlete experience was mixed, reflecting negative and positive experiences. Three themes were identified as impactful of the athlete experience: ableization, size of the parasport contingent, lack of unified collective voice. Practical implications : Future research that examines whose assumptions inform decision making and program delivery is necessary to determine the merits and drawbacks of integration in sport. Research contributions : By foregrounding the lived experiences of a group of parasport insiders, the research generates unique insights into the integrated model of sport from a perspective that is often missing, and credibly informs those who manage integration in sport.