In this present research, calcium phosphate cement (CPC) pastewas prepared by combining cement liquids comprised of 4 wt-% disodiumhydrogen orthophosphate with cement powders that consisted of β-tricalciumphosphate and monocalcium phosphate monohydrate. In the in vitrostudy, the release of gentamicin sulphate (GS) from standard cylindricalsamples of the prepared CPC immersion in phosphate buffered saline solutionwas measured by high performance liquid chromatography test over a periodof time. In addition, as a representative for biomedical properties, settingtime, injectability and compressive strength were measured in order to detectchanges when mixing CPC with GS. In addition, the pH values of the phosphatebuffered saline solution containing samples descended gradually until reachingequilibrium pH. Overall, the results from in vitro releasestudy showed that it reached its maximum level, which was 35% of theinitial value of the GS on day 15, suggesting no irreversible binding occurredbetween the cement paste and the antibiotic.