Gallic acid, active constituent of amla fruit its natural abundance with beneficial multi actions in body make them attractive for clinical applications. In present study, we focused on extracting, separating and characterizing gallic acid from amla and further formulated into chitosan nanoparticles, so bring it to increase its aqueous solubility and thereby bioactivity. Gallic acid nanoparticles were prepared by using poloxamer 407, chitosan and Glyceryl Monooleate (GMO) using probe sonicator and high pressure homogenization method. Prepared nanoparticles were characterized by particle size, zeta potential, DSC, XRD, SEM, entrapment efficiency, loading content, in-vitro release and stability study. They showed approximately 76.80% encapsulation of gallic acid with average size of 180.8 ± 0.21 nm, and zeta potential +24.2 mV. The cumulative in vitro drug release upto 24 hrs 77.16% was achieved suggesting that from all our findings, it can be concluded that work will facilitate extraction, design and fabrication of nanoparticles for protection and sustained release of gallic acid particularly to colonic region.