Event Abstract Back to Event Engineering an ultrasound activated implant that provides on demand BMP-2 release for bone regeneration Gazelle Crasto1, Norbert Kartner2, Cameron Clokie1, Morris F. Manolson2, 3 and Sean A. Peel1, 4 1 University of Toronto, Oral and Maxillofacial Surgery, Canada 2 University of Toronto, Dental Research Institute, Canada 3 University of Toronto, Department of Biochemistry, Canada 4 Induce Biologics Inc, Canada Introduction: Recombinant bone morphogenetic protein 2 (BMP) implants can be an effective alternative to autogenous bone grafts. These advantages are offset, however, by cost and safety concerns due to the high initial dose of BMP required to compensate for its rapid release from the implant. To address this, we developed a novel protein delivery system, where liposomes sequester BMP at the bone defect site, until it is released by application of clinical ultrasound (US). Materials and Methods: We screened and characterized physical, chemical and ultrasound release properties of nine different liposome formulations. In vitro, release of BMP from liposomes was assessed following US exposure by varying exposure, pressure and frequency of US. Bone induction by the BMP-liposomes was evaluated in vivo in a mouse muscle pouch model. 16 groups of 10 mice each were implanted with BMP-containing liposomes and subjected to different US exposure profiles: single US treatment on day 0, 7 or 14 or dual exposure to US on day 0 and 7, or day 7 and 14, or twice on day 0, day 7 or day 14. The mice were harvested 28 days post last US treatment and micro CT and histology were used to quantitatively and qualitatively measure newly induced bone. Results: In vitro, Increase in pressure, frequency and time resulted in increased release of bioactive BMP-2 from liposomes. In vivo, animal studies revealed only US triggered release of BMP-2 from liposomes induced bone formation. Most importantly no bone formation was detected in mice containing BMP-liposomes that did not receive US. Enhanced bone formation was observed in groups where implants received two US treatments (Fig. 2) on day 0 and 7 instead of a single treatment (Fig. 1) on day 0, 7 or 14 or two treatments on the same day . The study further demonstrates BMP-liposomes can be used to control deliver BMP, additionally multiple early timed release of BMP results in enhanced bone formation. Conclusions: We have developed an implant that induces bone formation on US triggered controlled release of BMP-2 from liposomes. Additionally multiple rounds of US exposure increased bone formation compared to single exposure. G.J.C. was supported by NSERC Ph.D. Scholarship, Canadian Arthritis Network- The Arthritis Society Ph.D. Scholarship, Gerald Baker Oral Surgery Scholarship, Faculty of Dentistry- Harron Scholarship, Ontario Graduate Student-Science and Technology Scholarship.; Funding was provided by Dental Faculty Research Grant (DFRG).; Materials were provided by Induce Biologics Inc. Keywords: Bone Regeneration, growth factor, nanoparticle, delivery Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Crasto G, Kartner N, Clokie C, Manolson MF and Peel SA (2016). Engineering an ultrasound activated implant that provides on demand BMP-2 release for bone regeneration. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02878 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gazelle Crasto Norbert Kartner Cameron Clokie Morris F Manolson Sean A Peel Google Gazelle Crasto Norbert Kartner Cameron Clokie Morris F Manolson Sean A Peel Google Scholar Gazelle Crasto Norbert Kartner Cameron Clokie Morris F Manolson Sean A Peel PubMed Gazelle Crasto Norbert Kartner Cameron Clokie Morris F Manolson Sean A Peel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.