Use Of Release Research Articles

DESIGN, DEVELOPMENT, AND CHARACTERIZATION OF LORATADINE SUSTAINED RELEASED BUCCAL FILM: IN VITRO AND IN VIVO STUDY IN BEAGLE DOGS

Objective: The aim of this work was to develop and evaluate bucco-adhesive films of Loratadine (LTD) for sustained release use. Methods: Design of twelve different sustained released buccal film formulas using Carbopol, pectin, sodium alginate, glycerol, carboxymethyl cellulose (CMC), hydroxypropyl cellulose (HPC), Gelatin, Hydroxyethyl cellulose (HEC), and Hydroxypropyl methylcellulose (HPMC) as mucoadhesive polymers. Films were evaluated for physicochemical properties, thickness, swelling, moisture content, drug content, in vitro dissolution. The pharmacokinetic parameters of optimal formula were evaluated in beagle dogs. Results: The selected film formula (F6) showed accepted content and muco-adhesion properties. The in vitro release study showed prolonged release of drug from films over 10 h in optimal formulation. The bioavailability studies performed using beagle dogs model showed that there was 113.45% increase in the AUC0-24 of selected film compared with oral market tablets. Conclusion: Bucco-adhesive films is a promising dosage form for improving the bioavailability of loratadine.

Certain aspects of exemption from serving a probationary sentence

The article examines certain aspects of the application to the convict of release from serving a probationary sentence. It is worth noting that recently there has been a tendency in the state to expand the scope of the application of punishment without deprivation of liberty as one of the directions of the criminal policy of our state. The author analyzes the criminal law, legislative and practical aspects of assigning supervision to persons released from serving a probationary sentence, and determines the strategic and tactical purpose of its implementation.
 In the course of the study, the essence of exemption from serving a sentence with probation was determined, its purpose, and it was also clarified what grounds are sufficient and necessary for exemption from serving a sentence with probation.
 The scientific positions of scientists who, in different years, studied “Exemption from serving a sentence with probation” from different positions were analyzed. It has been proven that among scientists who have studied the issue, there is no consensus regarding the legal nature and essence of such an institution as exemption from serving a sentence. The range of scientific positions starts from equating release from serving a sentence with probation and criminal punishment to the formation of an independent legal institution “Probation” in criminal legislation, which will provide for the legal grounds for the use of release from serving a sentence with probation and the consequences of applying this type of release from serving a sentence. Taking into account the assets of the modern science of criminal law, on the basis of the research results of such an institute, criminologists have given their own vision of the content of release from serving a sentence with probation.
 In order to improve this institution, preventive measures can be developed and implemented, which relate to the problems of control over the execution of criminal punishment.

Preparation of pH-responsive oxidized regenerated cellulose hydrogels compounded with nano-ZnO/chitosan/aminocyclodextrin ibuprofen complex for wound dressing

Recently, using oxidized regenerated cellulose (ORC) to build a hydrogel system on promoting healing in wounds has a fast-growing market. However, it remains a challenge to improve the degree of oxidation of regenerated cellulose (RC) and to prepare matrices that are uniquely responsive to the wound environment. Herein, highly oxidized aldehyde-based cellulose from porous RC was prepared by NaBH4-HCl swelling and then NaIO4 oxidation pathway. Chitosan (CS), ethylenediamine-cyclodextrin (EDA-CD) along with ORC have been used to construct hydrogel matrices that are pH-responsive and capable of controlled drug release for use as future wound dressings. And zinc oxide nanoparticles (ZnO NPs) with antimicrobial effect and ibuprofen (IBU) with analgesic effect were piggybacked into the hydrogel system. XRD was used to study the presence of ZnO. SEM was used to observe the surface structure of the prepared hydrogel. TEM was used to observe the particle size of the ZnO NPs. Meanwhile, the oxidation conditions of the ORC were explored. Furthermore, the mechanical, swelling, water retention, cytotoxicity, bacterial inhibition properties and treatment effect, which are closely related to the application of wound dressing, were carefully researched. The unique characteristics of prepared hydrogel, including pH-responsive degradability and sustained release properties of IBU, were also investigated.

Poly(l-lactic acid) Scaffold Releasing an α4β1 Integrin Agonist Promotes Nonfibrotic Skin Wound Healing in Diabetic Mice.

Skin wound healing is a highly complex process that continues to represent a major medical problem, due to chronic nonhealing wounds in several classes of patients and to possible fibrotic complications, which compromise the function of the dermis. Integrins are transmembrane receptors that play key roles in this process and that offer a recognized druggable target. Our group recently synthesized GM18, a specific agonist for α4β1, an integrin that plays a role in skin immunity and in the migration of neutrophils, also regulating the differentiated state of fibroblasts. GM18 can be combined with poly(l-lactic acid) (PLLA) nanofibers to provide a controlled release of this agonist, resulting in a medication particularly suitable for skin wounds. In this study, we first optimized a GM18-PLLA nanofiber combination with a 7-day sustained release for use as skin wound medication. When tested in an experimental pressure ulcer in diabetic mice, a model for chronic nonhealing wounds, both soluble and GM18-PLLA formulations accelerated wound healing, as well as regulated extracellular matrix synthesis toward a nonfibrotic molecular signature. In vitro experiments using the adhesion test showed fibroblasts to be a principal GM18 cellular target, which we then used as an in vitro model to explore possible mechanisms of GM18 action. Our results suggest that the observed antifibrotic behavior of GM18 may exert a dual action on fibroblasts at the α4β1 binding site and that GM18 may prevent profibrotic EDA-fibronectin-α4β1 binding and activate outside-in signaling of the ERK1/2 pathways, a critical component of the wound healing process.

Butyryl chitosan: Synthesis, characterization and evaluation of the sustained release performance as tacrolimus carrier

Chitosan and its water-soluble derivatives have been widely used in biomaterials, but their disadvantages have hindered their widespread application. In this study, butyryl chitosan, a liposoluble chitosan derivative, was prepared by introducing butyryl groups into chitosan molecules through an acylation reaction. The synthesis conditions were optimised, the reaction was more efficient and simpler than previously reported strategies. Two butyryl chitosan with degrees of substitution greater than two were synthesised. Chemical characterisations confirmed that the hydroxyl and primary amine groups in the backbone of chitosan were converted into amide and ester groups, respectively. The butyryl chitosan films have advantages such as high mechanical properties, long biodegradation time, proper surface charge, good cytocompatibility, and histocompatibility. Butyryl chitosan was used as carrier to prepare an implantable tacrolimus sustained release film. Characterization results showed that tacrolimus existed in an amorphous state in the release film. This work embodies an innovative formulation using tacrolimus-loaded carboxymethyl chitosan amorphous solid dispersions embedded butyryl chitosan film with added advantage of sustained release, an effective blood concentration in vivo for 11 days and a systemic exposure but lower intrapatient variability in tacrolimus trough levels. This work merits sustainable tacrolimus release use by providing application-based proofs and qualifies as a potential formulation for sustainable tacrolimus release system. We expect an improvement in terms of therapeutic compliance with positive effects on allograft outcomes.

Меры поощрения и взыскания, применяемые к осужденным, состоящим на учете уголовноисполнительных инспекций

The article discusses the results of a study conducted in May 2021 by the Research Institute of the Federal Penitentiary Service of Russia on the application of incentive and penalty measures against individuals sentenced to non-custodial punishments. In order to streamline the measures of disciplinary responsibility in relation to those sentenced to compulsory labor we suggest that the Penal Enforcement Code of the Russian Federation should contain a measure of punishment in the form of a warning, and for positive stimulation of lawabiding conduct – measures of encouragement in the form of gratitude and early removal of a previously imposed penalty in the form of a warning. In relation to those sentenced to restriction of liberty we propose to establish a provision in the law that they are maliciously evading from serving a sentence if they have committed a violation of the order and conditions of serving a sentence within one year after the application of a penalty in the form of an official warning, repeated punishment in the form of an official warning. Only after that, it is advisable to submit to the court a presentation about replacing the unserved term with a punishment in the form of imprisonment. It is proposed to provide for the use of release on parole as the main type of encouragement.

The impact of policy change on prisoner resettlement and community integration: A case of disproportionate response

The involvement of prisoners on license in the recent London Bridge and Streatham, London attacks have triggered a series of policies aiming to restrict community release. These aim to address not only the point at which prisoners in England and Wales are released, but also the level of engagement prisoners can have with the community before release. They have been introduced with little consultation of the available evidence and, seemingly, with little consideration of those who will be directly impacted as a result of their implementation. This commentary considers how announced changes in policy relating to the use of Release on Temporary Licence (ROTL) with prisoners, represent a response which is not only disproportionate to the scale of the problem but may also negatively impact upon prisoner rehabilitation. It draws upon evidence surrounding the impact of early release and evidence surrounding the practices which work to promote desistance from crime to highlight the flaws in these new policies, but also the importance of maintaining community engagement in the rehabilitation of people with convictions.

Photoprotection and release study of spinosad biopolymeric microparticles obtained by spray drying

Microparticles containing the photosensitive spinosad (SP) were obtained using a combination of chitosan (CH) and sodium lignosulfonate (SL) as encapsulating materials to develop a photoprotective system for intake administration. The microparticles were obtained by a spray drying technique and were characterized in terms of morphology (scanning electron microscopy - SEM) and particle size. In vitro release studies showed an initial burst effect that fits very well with the zero order kinetic model and then a slow release of the bioinsecticide, which fits best with the first order model. The results of this work demonstrate that the bioinsecticide carrier system developed with natural polymeric materials presents high stability to photodegradation and adequate insecticide release for use as a strategy to reduce negative impacts on the environment in agronomic practices.

Patterns of oxycodone controlled release use in older people with cancer following public subsidy of oxycodone/naloxone formulations: An Australian population-based study.

Public subsidy of the oxycodone/naloxone controlled release (CR) combination in December 2011 expanded the overall market for oxycodone CR in the general public in Australia; we evaluate its impact in people with cancer. We used Repatriation Pharmaceutical Benefits dispensing data linked with the NSW Cancer Registry for Department of Veterans' Affairs (DVA) healthcare card holders 65 years and older residing in NSW between 2004 and 2013 to identify clients with cancer and their opioid dispensings. We used interrupted time series analysis to model changes in monthly rates of oxycodone CR tablets dispensed and initiations. We performed a retrospective cohort study to examine changes in client characteristics and opioid utilization over time by comparing clients initiating oxycodone CR before and after subsidy. The rate of oxycodone CR tablets dispensed/month increased by 20% from December 2011, due to uptake of the oxycodone/naloxone CR combination; monthly initiations increased immediately by 17%. Initiations of buprenorphine, fentanyl, and morphine declined from December 2011. DVA healthcare card holders were significantly more likely to initiate the 5mg oxycodone CR formulation; more likely to use immediate release oxycodone in the 90 days following initiation; and less likely to use a weak opioid in the 90 days preceding oxycodone CR initiation following December 2011 than they were prior to that time. The public subsidy of the oxycodone/naloxone CR formulation expanded the overall oxycodone CR market for DVA healthcare card holders with cancer. Our findings highlight the need for updated guidelines around risk management for opioid treatment in patients with cancer.

Simplified source description for atmospheric dispersion model comparison of the Jack Rabbit II chlorine field experiments

This paper provides a summary of the methods used to quantify the mass release rate and thermodynamic state of the chlorine released during the Jack Rabbit II chlorine field experiments. In the two-year campaign, eight successful field trials were conducted in which chlorine was released in quantities of 5–10 tons (4540–9070 kg) through a 6-inch (0.152 m) circular breach in the test vessel (disseminator) along with an additional 20 ton (18100 kg) release (Trial 9). Previous analysis of load cell data has been conducted to quantify the mass release rate from primary containment as well as video evidence from Trial 6 which was used to quantify the rainout (and subsequent re-evaporation) during that trial. A method was developed to extend this analysis approach to other trials (except Trial 8 which was vertically upward and Trial 9 which was not instrumented with load cells). The objective of this work was to provide a simplified description of the source suitable for comparison of calculations made using different atmospheric transport and dispersion (ATD) models in Trials 1–7. This work also provides an estimate of the momentum flux (and mean velocity) of the release for use by ATD models which would use such information as input. The recommended release rate parameters are presented along with a summary of the relevant assumptions used in the analysis.

New hepatitis C diagnoses in Ontario, Canada are associated with the local prescription patterns of a controlled-release opioid.

Increases in acute hepatitis C virus (HCV) incidence may be a result of the rising prevalence of injection drug use and the opioid epidemic. Among persons who inject drugs, sharing of needles/syringes is less common and leads to a smaller proportion of incident cases than does sharing of injection drug preparation equipment. In Canada and Europe, hydromorphone controlled release has been associated with frequent reuse and sharing of IDPE. Drug excipients within HCR have been shown to preserve virus survival within IDPE. We hypothesized that regional differences in HCV incidence would mirror regional differences in HCR prescribing. We reviewed HCV incidence data across Ontario, Canada for 2016. Opioid prescribing patterns in each Health Unit were reviewed. Multivariable Poisson regression analyses were performed to test the strength of hydromorphone controlled release dispensing patterns in explaining HCV incidence compared to all opioids. Less vehicle access, lack of education, lower income, less population density, higher white race/ethnicity and more opioid substitution therapy recipients remained significant positive predictors of hepatitis C incidence in the Ontario model. Higher hydromorphone controlled release dispensing rate was a stronger predictor of HCV incidence than all opioid prescriptions (standardized risk ratio=1.17, P<.0001 vs sRR=1.11, P=.02). When hydromorphone controlled release was excluded from the opioid prescription variable, dispensing patterns of all other opioids no longer remained a significant predictor (sRR=1.042, P=.34). The observed relationship between HCV incidence and hydromorphone controlled release dispensing suggests that the type of opioid prescribed locally may contribute to variations in HCV incidence. These data add support to evidence that hydromorphone controlled release use is contributing to HCV spread in Ontario.

The Pharmacoepidemiology of Psychotropic Medication Use in Canadian Children from 2012 to 2016.

Objective: The goal of this study was to characterize the frequency and trends of psychotropic drug prescribing in Canadian children from 2010 to 2016 and to compare these results with a previous study conducted between 2005 and 2009. Methods: Using a national physician panel survey database from IQVIA Canada, aggregated frequencies of written prescriptions and therapeutic indications for antipsychotics, attention-deficit/hyperactivity disorder (ADHD) medications (psychostimulants and nonstimulants), and antidepressants were analyzed in children. Changes in frequency of written prescriptions and therapeutic indications are presented using descriptive statistics. Results: Written prescriptions for antipsychotics decreased by 10% from 2010 to 2016, in contrast to a 114% increase in written prescriptions for antipsychotics observed between 2005 and 2009. Written prescriptions for psychostimulants and antidepressants rose by 35% and 27%, respectively, between 2012 and 2016, comparable with previous results. The most common reasons for recommending an antipsychotic were ADHD and conduct disorder, although there appears to be a downward trend for ADHD compared with other conditions. In contrast, the share of written prescriptions for antipsychotics for autism increased 34% over the study period. Within the second-generation antipsychotics, written prescriptions for aripiprazole increased. An increase in the use of guanfacine extended release for ADHD was also observed. Conclusion: Several factors may be involved in stabilization and small decrease in antipsychotic use in recent years, including physician and patient awareness of adverse effects related to antipsychotic use, knowledge implementation strategies advocating short-term and judicious use of antipsychotics in children, and the approval of guanfacine extended release for use in Canada for ADHD in 2013.

Release on Parole: Criminal Legal and Penal Aspects

The article analyzes the material criteria for assessing the degree of correction of the sentenced to imprisonment according to a range of socially significant requirements necessary for a positive solution to the issue of release on parole. The authors provide statistics on the number of exempted from serving the sentence of imprisonment following the application for parole. Conclusions about the causes of the reduction of the total number of petitions to the Court made by the sentenced to imprisonment are drawn. The authors consider the problems of application of release on parole to the convicted having committed offences of low-to-medium severity and having spent long time in custody in a remand prison. They make suggestions on improvement of legal regulation on the use of release on parole for this category of convicts.

Notice of Release of 'Trailhead II basin wildrye

‘Trailhead II’ basin wildrye ( Leymus cinereus (Scribn. & Merr.) A. Love [Poaceae]) is a tetraploid basin wildrye release for use in re-vegetation efforts on rangelands of western North America. Trailhead II is the result of 2 cycles of recurrent selection within the basin wildrye cultivar ‘Trailhead’ for rapid seedling emergence from deep seeding. Trailhead II is genetically distinct from Trailhead. Additionally, in comparisons between Trailhead II and Trailhead, Trailhead II possessed higher total emergence and more rapid emergence from deep seeding (7.6 cm [3 in]) and exhibited higher seedling establishment at 3 of 5 field sites where it was evaluated in Nevada, Utah, and Wyoming. The release of Trailhead II basin wildrye improves this native germplasm to enhance the success of conservation and re-vegetation plantings in the Intermountain West and Northern Great Plains areas of the US and Canada.

Development and characterization of alginate microcapsules containing Bifidobacterium BB-12 produced by emulsification/internal gelation followed by freeze drying

Emulsification/internal gelation is an encapsulation method showing great potential to confer protection on probiotics that need to be added to foods with an unfavorable environment for their viability. The objective of this study was to produce probiotic microcapsules by internal ionic gelation associated with freeze drying, and evaluate the viability of the probiotics under simulated gastrointestinal conditions and their stability during storage. The results showed that microencapsulation culminated in a elevated viability of the probiotics and that the microcapsules were resistant to the simulated gastrointestinal conditions, providing substantial protection to Bifidobacterium BB-12. The encapsulated microorganisms maintained their viability of 7.88 ± 0.05 log CFU g−1 for up to 60 days at 25 °C, but the best results were conserved when submitted to refrigerated (7 °C) or frozen (−18 °C) storage. In addition to the microcapsules remained protected in a solution buffered at pH 4.5 but exposed total release of the probiotics in a solution buffered at pH 7.5, demonstrating conditions of controlled release for use with probiotic carriers for human consumption. Thus, the study demonstrated that emulsification/internal ionic gelation associated with the freeze drying process could be considered feasible technology for the protection, application and controlled release of probiotics for use in foods.

High-Frequency Ultrasonic Imaging of Growth and Development in Manufactured Engineered Oral Mucosal Tissue Surfaces

This study uses high-resolution ultrasound to examine the growth and development of engineered oral mucosal tissues manufactured under aseptic conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm, and oral keratinocytes seeded onto AlloDerm to form an ex vivo-produced oral mucosal equivalent (EVPOME) suitable for intra-oral grafting. The seeded cells produce a keratinized protective upper layer that smooths out any remaining surface irregularities on the underlying AlloDerm. Two-dimensional acoustic imaging of unseeded AlloDerm and developing EVPOMEs was performed on each day of their growth and development, each tissue specimen being imaged under aseptic conditions (total time from seeding to maturation: 11 d). Ultrasonic monitoring offers us the ability to determine the constituents of the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue-engineered constructs before release for use in patient care.

Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank

Human amniotic membrane (hAM) is known to have good potential to help the regeneration of tissue. It has been used for over 100 years in many medical disciplines because of its properties, namely a scaffold containing stem cells and growth factors, with low immunogenicity and anti-microbial, anti-inflammatory, anti-fibrotic and analgesic properties. In order to use this "boosted membrane" as an advanced therapeutic medicinal product for bone repair, we aimed to observe the influence of tissue culture and/or cryopreservation on cell viability and tissue structure, and secondly, to adapt to a tissue bank, identify easy processes to store hAM containing viable cells and to verify the quality of the graft before its release for use. To this end, we tested different published culture or cryopreservation storage conditions and cell viability assays. Tissue structure was evaluated by Giemsa staining and was compared to histological analysis. Preliminary results show no dramatic decrease in cell viability in cultured hAM as compared to cryopreserved hAM, but tissue structure alterations were observed with both storage conditions. Histological and immunohistochemical data highlight that tissue damage was associated with significantly modified protein expression, which could lead to a possible loss of differentiation potential. Finally, we report that trypan blue and Giemsa staining could constitute controls that are "materially and easily transferable" to a tissue bank.

Preliminary exploration of the development of a collagenous artificial dura mater for sustained antibiotic release

Background Intracranial infection is one of the most common complications of open craniocerebral injury and of conventional craniotomy in neurosurgery. The presence of blood-brain barrier leads to lower drug concentrations in the cerebrospinal fluid than in the venous blood. Increasing the intravenous dosage or frequency carries the risk of systemic adverse reactions or infections in other parts of the body. Developing an artificial dura mater (ADM) for sustained antibiotic release for use during neurosurgery can solve the problems perfectly. Methods Three types of drug-loaded ADMs made of collagen and containing cefuroxime sodium, ceftriaxone sodium, or norvancomycin were prepared. The antibacterial activity and sustained release characteristics of the ADMs were examined using bacteriostatic and release tests. Results Single-layered collagen based ADMs (40 mm×50 mm×5 mm) containing 18 mg cefuroxime sodium or ceftriaxone sodium were not suitable for continued development because of drug preservation and stability issues. Using smaller ADMs (20 mm×30 mm×7 mm), containing 4.86 mg of norvancomycin, with increased collagen density and a three-layered film with two outer drug-free films above and below the antibiotic layer resulted in sustained cumulative release of 2.91 mg (59.9%) of norvancomycin over 72 hours. The similar factor (f2) comparison method proved that products from a same batch were statistically significant similar (f2 &gt;50). Conclusions Artificial ADMs made of collagen can be processed to provide a mature dural repair material for the sustained release of norvancomycin. This system may provide a basis for developing sustained release materials for other drugs.

One-Pot Construction of Functional Mesoporous Silica Nanoparticles for the Tumor-Acidity-Activated Synergistic Chemotherapy of Glioblastoma

Mesoporous silica nanoparticles (MSNs) have proved to be an effective carrier for controlled drug release and can be functionalized easily for use as stimuli-responsive vehicles. Here, a novel intelligent drug-delivery system (DDS), camptothecin (CPT)-loaded and doxorubicin (DOX)-conjugated MSN (CPT@MSN-hyd-DOX), is reported via a facile one-pot preparation for use in synergistic chemotherapy of glioblastoma. DOX was conjugated to MSNs via acid-labile hydrazone bonds, and CPT was loaded in the pores of the MSNs. At pH 6.5 (analogous to the pH in tumor tissues), a fast DOX release was observed that was attributed to the hydrolysis of the hydrazone bonds. In addition, a further burst release of DOX was found at pH 5.0 (analogous to the pH in lyso/endosomes of tumor cells), leading to a strong synergistic effect. In all, CPT and DOX could be delivered simultaneously into tumor cells, and this intelligent DDS has great potential for tumor-trigged drug release for use in the synergistic chemotherapy of tumors.

Characterization of fast-decaying PET radiotracers solely through LC-MS/MS of constituent radioactive and carrier isotopologues

BackgroundThe characterization of fast-decaying radiotracers that are labeled with carbon-11 (t1/2 = 20.38 min), including critical measurement of specific radioactivity (activity per mole at a specific time) before release for use in positron-emission tomography (PET), has relied heavily on chromatographic plus radiometric measurements, each of which may be vulnerable to significant errors. Thus, we aimed to develop a mass-specific detection method using sensitive liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) for identifying 11C-labeled tracers and for verifying their specific radioactivities.MethodsThe LC-MS/MS was tuned and set up with methods to generate and measure the product ions specific for carbon-11 species and M + 1 carrier (predominantly the carbon-13 isotopologue) in four 11C-labeled tracers. These radiotracers were synthesized and then analyzed before extensive carbon-11 decay. The peak areas of carbon-11 species and M + 1 carrier from the LC-MS/MS measurement and the calculated abundances of carbon-12 carrier and M + 1 radioactive species gave the mole fraction of carbon-11 species in each sample. This value upon multiplication with the theoretical specific radioactivity of carbon-11 gave the specific radioactivity of the radiotracer.ResultsLC-MS/MS of each 11C-labeled tracer generated the product ion peaks for carbon-11 species and M + 1 carrier at the expected LC retention time. The intensity of the radioactive peak diminished as time elapsed and was undetectable after six half-lives of carbon-11. Measurements of radiotracer-specific radioactivity determined solely by LC-MS/MS at timed intervals gave a half-life for carbon-11 (20.43 min) in excellent agreement with the value obtained radiometrically. Additionally, the LC-MS/MS measurement gave specific radioactivity values (83 to 505 GBq/μmol) in good agreement with those from conventional radiometric methods.Conclusions11C-Labeled tracers were characterized at a fundamental level involving isolation and mass detection of extremely low-abundance carbon-11 species along with the M + 1 carrier counterpart. This LC-MS/MS method for characterizing fast-decaying radiotracers is valuable in both the development and production of PET radiopharmaceuticals.