In this paper, we propose a destination-assisted jamming and beamforming (DAJB) scheme for physical layer security in a one-way cooperative amplify-and-forward (AF) relay communication system. The system model consists of one source, one destination, one eavesdropper, and N relay nodes, with the individual power constraint of each relay node and the destination-assisted jamming node as well as unknown instantaneous channel state information (CSI) of the eavesdropper. Due to the half-duplex constraint of the relay nodes, there are two phases in our proposed DAJB scheme. In the first phase, the source node broadcasts information signal and all N relay nodes listen simultaneously. In the meantime, the destination node transmits the jamming signal to confuse the potential eavesdropper. In the second phase, all N relay nodes amplify and forward the received signals, covered by the artificial noise (AN), to the destination using the distributed beamforming technology. The optimal beamformer weights and power allocation are obtained by solving the second-order convex cone programming (SOCP) together with a linear programming (LP) problem. Furthermore, the performance of the DAJB scheme is analyzed in terms of the achievable secrecy rate. Compared with the scheme that selects a relay node as the jammer, we obtain larger power gain to achieve higher secrecy rates. Finally, the simulation results verify that the DAJB scheme greatly improves the secrecy rate of a one-way cooperative AF relay communication system.