The integration of wireless power transfer (WPT) with the low-power backscatter communications provides a promising way to sustain battery-less wireless networks. In this paper, we consider a backscatter communication network wirelessly powered by a power beacon station (PBS). Each backscatter radio uses the harvested energy to power its data transmissions, in which some other radios can help as the wireless relays with an aim to improve throughput performance by cooperative transmission. Under this setting, we formulate a throughput maximization problem to jointly optimize WPT and the relay strategy of the backscatter radios. An iterative algorithm with reduced complexity and communication overhead is proposed to decompose the original problem into two sub-problems distributed at the PBS and the backscatter receiver. Moreover, we take uncertain channel information into consideration and formulate robust counter-parts of the throughput maximization problem when either the backscatter or relay channel is subject to estimation errors. The difficulty of the robust counter-part lies in the coupling of the PBS’ power allocation and relay strategy in matrix inequalities, which is addressed by alternating optimization with guaranteed convergence. Numerical results reveal that the cooperative relay strategy of the backscatter radios significantly improves the throughput performance.