Related Factors Research Articles

Multilevel Analysis on Spatial Distribution and Socio-Environmental Factors of Dental Caries in Korean Children

This study aims to identify the regional distribution in the prevalence of dental caries and related multidimensional factors among 12-year-old children in Korea. Data from the 2018 Child Oral Health Survey were used to calculate the average DMFT index of 12-year-old children in metropolitan cities, and a multi-level regression model was applied to explain the regional distribution of dental caries prevalence and related factors. Factors were divided into two levels by administrative structure. This study finds a significant regional difference in the prevalence of dental caries in 12-year-old Korean children across metropolitan cities. Multilevel analysis showed that districtlevel factors (average number of pit and fissure-sealed permanent teeth, dental treatment demand rate, preventive treatment rate, sex ratio, and number of dentists per 100,000 people) and metropolitan-level factors (intakes of cariogenic beverages and number of pediatric dental hospitals and clinics per 100,000 people) had a significant effect on dental caries prevalence (<i>p</i> < 0.05). Individual characteristics and local socio-environmental factors influence the prevalence of dental caries. Especially considering the strong dependence on preventive treatment and accessibility to dental care services, it is necessary to provide adequate preventive treatment and expand health care resources in high-risk areas of dental caries.

Nurses’ healthy behaviors during the COVID-19 pandemic and related factors

Purpose: This study aimed to examine nurses’ healthy behaviors during the coronavirus disease 2019 (COVID-19) pandemic and related factors.Methods: A cross-sectional study was adapted, and data were collected from 300 hospital nurses between August and November 2021. The nurses’ characteristics, healthy behaviors, COVID-19 stress levels, health self-efficacy, and nursing professional pride were self-reported using structured questionnaires. Multivariable linear regressions were conducted to identify factors related to nurses’ healthy behaviors.Results: Healthy lifestyle was the lowest among the subscales of healthy behaviors. Nurses’ healthy behaviors were related to age (B=0.15, p=.021), COVID-19 stress level (B=-0.08, p=.007), nursing professional pride (B=0.19, p<.001), and health self-efficacy (B=0.38, p<.001).Conclusion: To enhance nurses’ healthy lifestyles during the pandemic, organizational support is needed, such as ensuring facilities for rest or physical activities accessible from the hospital and supplying healthy food in hospitals. Younger nurses, nurses with high levels of COVID-19 stress, and nurses with lower health self-efficacy may benefit from hospital organizations that provide more support and guidance in promoting health behaviors. Furthermore, hospital organizations should promote professional pride by empowering nurses’ efforts and reinforcing their values.

Evaluation of Design Parameters for Daylighting Performance in Secondary School Classrooms Based on Field Measurements and Physical Simulations: A Case Study of Secondary School Classrooms in Guangzhou

The quality of natural lighting within secondary school classrooms can significantly affect the physical and mental well-being of both teachers and students. While numerous studies have explored various aspects of daylighting performance and its related factors, there is no universal standard for predicting and optimizing daylighting performance from a design perspective. In this study, a method was developed that combines measurements and simulations to enhance the design parameters associated with daylighting performance. This approach facilitates the determination of precise ranges for multiple design parameters and allows for the efficient attainment of optimal daylighting performance. Daylight glare probability (DGP), point-in-time illuminance (PIT), daylight factor (DF), and lighting energy consumption were simulated based on existing control parameters of operational classrooms. The simulation results were then validated using field measurements. Genetic algorithms (GAs) were employed to optimize the control parameters, yielding a set of optimal solutions for improving daylight performance. The differences between daylighting performance indicators corresponding to the optimal solution set and those of the basic model were compared to test the performance of the optimized parameters. The proposed method is a robust process for optimizing daylight design parameters based on GAs, which not only enhances daylighting performance but also offers scientifically grounded guidelines for the design phase. It is a valuable framework for creating healthier and more productive educational environments within secondary school classrooms.

The prevalence of anxiety and related factors among primary and secondary school teachers in Hanoi, Vietnam, during the COVID-19 pandemic in 2020.

The working conditions for teachers in Vietnam were characterized by increased workload and pressure, burdening teachers' well-being. The study aims to investigate anxiety prevalence and identify some related factors among primary and secondary school teachers in Hanoi after the first COVID-19 outbreak in 2020. This paper analyzed data of 481 teachers working at ten primary and secondary schools in Hanoi city. Anxiety was measured using the anxiety component of the Depression, Anxiety, and Stress scale 42 items. Multivariable logistics regression was performed to examine anxiety-related factors using SPSS 20.0 at a significant level p less than 0.05. The prevalence of anxiety symptoms was 42.4% and similar between primary and secondary school teachers. More secondary teachers reported moderate to severe anxiety symptoms than primary teachers did (31.6% and 27.7%). Primary school teachers who felt discomfort with their supervisor's assessment, high responsibility for student safety, and ever thinking of leaving their current job were more likely to report anxiety symptoms (OR (95%CI) = 2.8 (1.2-6.5), 3.6 (1.0-12.8), and 2.6 (1.3-5.4), respectively). Meanwhile, the discomfort of caring for many students or problematic students, repetitive work, and disagreement with coworkers were risk factors of anxiety among secondary school teachers (OR (95%CI) = 2.6 (1.2-5.8), 3.2 (1.1-9.2), 3.4 (1.3-8.8), and 3.7 (1.1-12.6), respectively). In conclusion, the prevalence of teachers with anxiety symptoms is on the rise, caused by the characteristics of the job and professional relationships. Tailored support for teachers in different grades is necessary to improve and prevent teachers' anxiety.

Structure-Activity Relationship of PAD4 Inhibitors and Their Role in Tumor Immunotherapy.

Protein arginine deiminase 4 (PAD4) plays an important role in cancer progression by participating in gene regulation, protein modification, and neutrophil extracellular trap (NET) formation. Many reversible and irreversible PAD4 inhibitors have been reported recently. In this review, we summarize the structure-activity relationships of newly investigated PAD4 inhibitors to bring researchers up to speed by guiding and describing new scaffolds as optimization and development leads for new effective, safe, and selective cancer treatments. In addition, some recent reports have shown evidence that PAD4 inhibitors are expected to trigger antitumor immune responses, regulate immune cells and related immune factors, enhance the effects of immune checkpoint inhibitors, and enhance their antitumor efficacy. Therefore, PAD4 inhibitors may potentially change tumor immunotherapy and provide an excellent direction for the development and clinical application of immunotherapy strategies for related diseases.

Anticoagulant Rodenticide Toxicity in Terrestrial Raptors: Tools to Estimate the Impact on Populations in North America and Globally.

Anticoagulant rodenticides (ARs) have caused widespread contamination and poisoning of predators and scavengers. The diagnosis of toxicity proceeds from evidence of hemorrhage, and subsequent detection of residues in liver. Many factors confound the assessment of AR poisoning, particularly exposure dose, timing and frequency of exposure, and individual and taxon-specific variables. There is a need, therefore, for better AR toxicity criteria. To respond, we compiled a database of second-generation anticoagulant rodenticide (SGAR) residues in liver and postmortem evaluations of 951 terrestrial raptor carcasses from Canada and the United States, 1989 to 2021. We developed mixed-effects logistic regression models to produce specific probability curves of the toxicity of ∑SGARs at the taxonomic level of the family, and separately for three SGARs registered in North America, brodifacoum, bromadiolone, and difethialone. The ∑SGAR threshold concentrations for diagnosis of coagulopathy at 0.20 probability of risk were highest for strigid owls (15 ng g-1 ) lower and relatively similar for accipitrid hawks and eagles (8.2 ng g-1 ) and falcons (7.9 ng g-1 ), and much lower for tytonid barn owls (0.32 ng g-1 ). These values are lower than those we found previously, due to compilation and use of a larger database with a mix of species and source locations, and also to refinements in the statistical methods. Our presentation of results on the family taxonomic level should aid in the global applicability of the numbers. We also collated a subset of 440 single-compound exposure events and determined the probability of SGAR-poisoning symptoms as a function of SGAR concentration, which we then used to estimate relative SGAR toxicity and toxic equivalence factors: difethialone, 1, brodifacoum, 0.8, and bromadiolone, 0.5. Environ Toxicol Chem 2024;00:1-11. © 2024 His Majesty the King in Right of Canada and The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC Reproduced with the permission of the Minister of Environment and Climate Change Canada.

Quantification of secondary ion mass spectrometry measurements by using ion‐implanted metallic standards

This research addresses an analytical methodology to quantify elements of interest in fusion‐relevant materials using secondary ion mass spectrometry (SIMS). For this purpose, internal standards have been fabricated by ion implantation to avoid the well‐known matrix effect of this technique. In particular, chromium has been implanted at an energy of 12 MeV using two fluences in high‐purity iron and tungsten matrices together with Si control substrates. The latter were applied to determine the Cr concentration implanted through experimental and semiempirical methods. Specifically, the IBA technique Rutherford backscattering spectrometry (RBS) provided the quantitative results being 3.1 × 1019 at/cm3 and 1.6 × 1019 at/cm3 for the high and low dose, respectively. The SIMS depth profiles of Cr for the Fe and W matrices established an ion implantation depth close to 2 μm on both substrates in agreement with the calculations previously performed by Stopping and Range of Ions in Matter (SRIM) simulations. Correlation between the integration of SIMS profiles and known concentrations of the implanted ion resulted in the calibration curve for each matrix, obtaining the SIMS quantification approach by means of this relative sensitivity factor (RSF). Additionally, a cross‐check of the method by comparing commercial Fe‐Cr alloys with the Cr‐implanted Fe matrices of the present study pointed out the need to produce standards with higher chromium concentrations.

Prevalence, severity, and risk factors of cancer-related fatigue among working cancer survivors: a systematic review and meta-analysis.

The aim of this study is to evaluate the prevalence, severity, and risk factors of cancer-related fatigue among currently working cancer survivors. We searched the PubMed, Embase, Scopus, CINAHL, Cochrane Library, and ICHUSHI databases. The risk of bias was evaluated independently using the Risk of Bias Assessment Tool for Non-randomized Studies (RoBANS). A meta-analysis was conducted to determine the prevalence, severity, and related factors associated with cancer-related fatigue among currently working cancer survivors. Our meta-analysis included 18 studies and revealed that 42.2% of currently working cancer survivors experience cancer-related fatigue. The fatigue severity in this group was significantly higher than that in workers without cancer (absolute standardized mean difference (SMD) = 0.67), but lower than that in cancer survivors who had previously worked and were not currently working (absolute SMD = 0.72). Distress was identified as a potential risk factor for cancer-related fatigue in working cancer survivors (partial correlation coefficient = 0.38). The high prevalence of cancer-related fatigue among employed cancer survivors underscores the need for targeted workplace interventions and fatigue management strategies. While the severity of fatigue is less than that seen in non-working survivors, the comparison with the general working population highlights a significant health disparity. The association between distress and fatigue suggests the necessity for a holistic approach to fatigue management that considers both physical and mental factors in working cancer survivors. Our findings highlight the critical need for healthcare professionals and employers to monitor fatigue levels among working cancer survivors and offer appropriate support.

중학교 교사의 인권침해경험과 교사효능감의 관계: 수업효능감과 생활지도효능감을 중심으로

Based on the data of middle school teachers in 2021 from the Gyeonggi School Education Survey, this study explored the actual situation and related factors of teachers' human rights violation experiences and analyzed the relationship with teacher efficacy (instructional efficacy and life guidance efficacy). The results showed that the majority of middle school teachers had experienced human rights violations by students, and that teacher gender, teaching experience, school climate, and the average number of hours per week of students in the classroom were related to this. When other factors were considered, it was found that teachers who had experienced human rights violations by students had significantly lower teaching effectiveness and life guidance effectiveness.
 Based on these results, we suggest the following implications for teacher policies in Korea. First, the concept of teaching rights should be expanded to include the basic human rights of teachers in educational activities and include violations of teachers' human rights in addition to violations of teaching and life guidance. Second, existing data on teacher rights violations do not adequately reflect the full range of teacher rights violations, so it is necessary to improve them. Third, since the degree of human rights violation negatively affects teachers' sense of teacher effectiveness, it is necessary to actively protect teachers and provide psychological support and training so that they can regain confidence in teaching and life guidance

중학교 교사의 인권침해경험과 교사효능감의 관계: 수업효능감과 생활지도효능감을 중심으로

Based on the data of middle school teachers in 2021 from the Gyeonggi School Education Survey, this study explored the actual situation and related factors of teachers' human rights violation experiences and analyzed the relationship with teacher efficacy (instructional efficacy and life guidance efficacy). The results showed that the majority of middle school teachers had experienced human rights violations by students, and that teacher gender, teaching experience, school climate, and the average number of hours per week of students in the classroom were related to this. When other factors were considered, it was found that teachers who had experienced human rights violations by students had significantly lower teaching effectiveness and life guidance effectiveness.
 Based on these results, we suggest the following implications for teacher policies in Korea. First, the concept of teaching rights should be expanded to include the basic human rights of teachers in educational activities and include violations of teachers' human rights in addition to violations of teaching and life guidance. Second, existing data on teacher rights violations do not adequately reflect the full range of teacher rights violations, so it is necessary to improve them. Third, since the degree of human rights violation negatively affects teachers' sense of teacher effectiveness, it is necessary to actively protect teachers and provide psychological support and training so that they can regain confidence in teaching and life guidance

Adherence to Medications in Patients with Ischaemic Heart Disease in Oman.

This study aimed to evaluate the level of adherence to medication among patients with ischaemic heart disease (IHD) in Oman and assess the related factors. This cross-sectional questionnaire-based study among patients with IHD attending the outpatient clinic at Sultan Qaboos University Hospital, Muscat, Oman, was performed between January and December 2021. A total of 105 patients (mean age = 49.9 ± 11.1 years, 78.1% male) were recruited. Most of the patients (80%) reported taking the medications by themselves; 77 (73.3%) patients said that over the preceding 2 weeks, they had missed at least 3 doses of their medication. The reasons for missing the medications included forgetting (100%), having to take too many tablets (57%), feeling that the tablets are not effective (48%) and having to take the tablets too often each day (23%). The factors responsible for patients failing to take medications could not be identified. Medication adherence was low among patients with IHD in Oman, with high pill burden being the most common reason for non-adherence. Physicians must bear this in mind when reviewing patients.

Interpersonal behaviors in sports context: Spanish adaptation and measurement invariance.

The interpersonal behavior questionnaire (IBQ) is an instrument that measures support and thwarting interpersonal behaviors based on the self-determination theory (SDT). The aim of this work was to adapt the IBQ to the Spanish spoken in Mexico and to examine its psychometric properties (structural validity, discriminant validity, composite reliability, factorial invariance, and nomological validity) in a sample of athletes. For this purpose, 472 athletes (average age 17.15 years; SD = 1.47) completed a question booklet. Confirmatory factor analysis supported the structure of six related factors, three factors of behaviors that support autonomy, competence, and relatedness, and three factors of behaviors that thwarting them. The internal consistency of each factor was also supported, as well as the average variance extracted. However, the discriminant validity between the factors of competence and relatedness in their dimensions of support, on the one hand, and thwarting, on the other, is questioned. Factorial invariance was confirmed across gender (men and women) and sport type (individual and team). Nomological validity is in accordance with theory and empirical literature. More studies of the IBQ in sport are necessary to see if these results are a fortuitous product or if they manifest themselves consistently.

Risk factors for short-term all-cause mortality in patients with end stage renal disease: a scoping review

ObjectivesThere is a lack of prognostic information to guide the prediction of short-term all-cause mortality in patients with end-stage renal disease (ESRD). The aim was to review the risk factors that influenced the risk of short-term all-cause mortality in patients with ESRD.MethodsMEDLINE, Embase, PubMed, CINAHL, the Cochrane Library and Web of Science databases were searched for articles published between 2000 and 2020. Articles describing risk factors predicting short-term mortality (≤ 3 years) in patients with ESRD were included. Four reviewers independently performed title, abstract, full text screening and data extraction. Assessment of risk of bias was assessed using the Quality In Prognosis Studies (QUIPS) tool checklist.Results20,840 articles were identified and 113 papers were included for this review. Of the 113 papers, 6.2% included only peritoneal dialysis (PD) patients, 67.3% included only hemodialysis (HD) patients, 20.4% included both PD and HD patients, with the remaining papers featuring patients on conservative management or awaiting renal transplant. Risk factors were categorised into 13 domains: 1)demographics/ lifestyle, 2) comorbidities 3)intradialytic blood pressure, 4)biomarkers, 5)cardiovascular measurements, 6)frailty status, 7)medications, 8)treatment related indicators, 9)renal related parameters, 10)health status, 11)cause of ESRD, 12)access to healthcare care/ information and, 13)proxy measures for poor health. C-reactive protein(CRP), age, and functional status were observed to have higher percentage of instances of being significantly associated with all-cause mortality.ConclusionCommonly examined risk factors observed from this review may be used to build a general prognostic model for patients with ESRD, with specific treatment related risk factors added on to enhance the accuracy of the models.

Work productivity loss and work-related factors during the first wave of the COVID-19 pandemic: A cross-sectional web-based survey.

The COVID-19 pandemic could have led to a technological revolution however it has caused work productivity loss, especially among people working from home. This study examined the frequency and related factors of work productivity loss, focusing on work-related factors, during the first wave of the COVID-19 pandemic in Japan. We conducted a cross-sectional, web-based survey of Japanese workers. Questionnaires were distributed using snowball sampling through e-mail and social networking sites. Of the respondents, 708 non-medical workers reported subjective work productivity loss during the first wave of the pandemic, compared with before. A multiple regression analysis showed that living with preschool-age children, having poor mental health, not using video conferencing solutions, and increased e-mail communication were related to work productivity loss. A subgroup analysis of those working from home showed similar variables associated with work productivity loss. More than 70% of workers in Japan experienced work productivity loss during the first wave of the pandemic. Moreover, factors such as time-consuming tasks or background distractions might lead to operational inefficacy.

Built or Social environment? Effects of perceptions of neighborhood green spaces on resilience of residents to heat waves

Large cities in North China are at an increasing risk of heat waves. Several previous studies have emphasized the significance of heat wave resilience but they generally failed to sufficiently consider the social level and to explore the mechanisms involved. In the present study, we investigated heat wave resilience as a full-cycle process and elucidated the related factors at the level of environmental perception. We selected 60 neighborhoods that are vulnerable to high temperatures in the central urban area of Tianjin. Using a questionnaire (N = 734), we evaluated the residents' perceptions in terms of the built-environmental perception (BEP) and socio-environmental perception (SEP) of neighborhood green spaces, as well as assessing the resilience levels of residents in the three phases comprising heat wave preparation, resistance, and recovery. Structural equation models were constructed to identify the mechanisms that influenced resilience in each phase. The results demonstrated a significant and direct effect of SEP on heat wave preparation and on the resistance phase. In addition, both BEP and SEP were critical for the recovery of residents. Using multi-group analysis, we identified differences in the mechanisms that allowed residents to acquire heat wave resilience. Based on these findings, we suggest that green space planning and policies should focus on optimizing the built environment, but also on the roles of residents' social support networks because they are crucial for guaranteeing the sustainability of vulnerable groups and helping communities to cope with heat waves.

Effects of vitamin D supplementation on liver fibrogenic factors, vitamin D receptor and liver fibrogenic microRNAs in metabolic dysfunction-associated steatotic liver disease (MASLD) patients: an exploratory randomized clinical trial

Background and aimsMetabolic dysfunction-associated steatotic liver disease (MASLD) is a global metabolic problem which can lead to irreversible liver fibrosis. It has been shown that vitamin D and its receptors contribute to fibrogenic pathways in the liver. However, the effect of vitamin D supplementation on liver fibrosis related factors have not been examined. This double blinded placebo controlled clinical trial was designed to investigate the effects on vitamin D supplementation on serum levels of VDR, fibrogenic factors and fibrogenic MicroRNAs in MASLD patients.MethodsForty six MASLD patients after block matching for sex and BMI were randomly assigned to receive 4000 IU/d vitamin D or placebo for 12 weeks. Weight, height and waist circumference were measured. Serum fibrogenic microRNAs, laminin, collagen type IV, hyaluronic acid, vitamin D, VDR, PTH, blood fasting glucose, serum fasting insulin, lipid profile, ALT and AST were determined at the baseline and at the end of the trial. Insulin resistance and insulin sensitivity were calculated using the HOMA-IR and QUICKI equation.ResultsSupplementation with vitamin D for 12 weeks led to the significant increases in serum 25(OH) vitamin D, VDR and HDL-C compared to placebo (P < 0.001, P = 0.008 and P < 0.001). There were significant decreases in ALT, AST, FBS and LDL-C levels in the vitamin D group as compared to the placebo (P < 0.05). Laminin and hyaluronic acid concentrations were significantly decreased in the vitamin D group as compared to the placebo group, by -10.6 and − 28.7 ng/mL, respectively. Supplementation with vitamin D for 12 weeks resulted in a significant lower MiR-21 and MiR-122 gene expressions compared to the placebo group (P = 0.01 and P < 0.001, respectively).DiscussionAs the first randomized controlled trial on the effect of vitamin D supplementation on serum levels of VDR, fibrogenic factors and fibrogenic MicroRNAs in MASLD patients, we found a significant reduction in some liver fibrogenic factors, in liver transaminases and corresponding changes in some fibrosis-related MiRs and some metabolic factors. Further clinical trials with larger sample sizes and direct measures of liver fibrosis are needed to confirm these findings.Trial registration number(available at: http://www.irct.ir, identifier: IRCT201405251485N13), Registration date: 14-03-2017.

Investigation of factors regarding the effects of COVID-19 pandemic on college students’ depression by quantum annealer

Diverse cases regarding the impact, with its related factors, of the COVID-19 pandemic on mental health have been reported in previous studies. In this study, multivariable datasets were collected from 751 college students who could be easily affected by pandemics based on the complex relationships between various mental health factors. We utilized quantum annealing (QA)-based feature selection algorithms that were executed by commercial D-Wave quantum computers to determine the changes in the relative importance of the associated factors before and after the pandemic. Multivariable linear regression (MLR) and XGBoost models were also applied to validate the QA-based algorithms. Based on the experimental results, we confirm that QA-based algorithms have comparable capabilities in factor analysis research to the MLR models that have been widely used in previous studies. Furthermore, the performance of the QA-based algorithms was validated through the important factor results from the algorithms. Pandemic-related factors (e.g., confidence in the social system) and psychological factors (e.g. decision-making in uncertain situations) were more important in post-pandemic conditions. Although the results should be validated using other mental health variables or national datasets, this study will serve as a reference for researchers regarding the use of the quantum annealing approach in factor analysis with validation through real-world survey dataset analysis.

Predictors of COVID-19 vaccine uptake among people who use substances: a case study in Tehran

BackgroundVaccination is one of the most effective ways to manage infectious disease epidemics such as Covid-19. However, the low rates of vaccination in populations at risk including people using illicit substances, hinders the effectiveness of preventive vaccines in reducing transmission. The aim of this study was to investigate the rate of Covid-19 vaccination and its related factors among people who use substances in Tehran, Iran.MethodsBetween July and December 2022, 386 people who use substances aged ≥ 18 years old were recruited by convenience street-based sampling in Tehran. The outcome variable in this study was self-reported completion of at least two doses of the Covid-19 vaccine. Logistic regression was used to investigate the factors related to Covid-19 vaccination. Data were analyzed using SPSS software version 20 at the 0.05 level of significance. As a measure of risk, 95% Confidence interval (CI) was used. The level of significance was considered at 0.05.ResultsAlmost three-quarters (n = 286) of the participants reported receiving at least two doses of the Covid-19 vaccine (95% CI, 70.2–79.3). Those participants with high school diplomas were 1.17 times more likely than less educated participants to report having had 2 vaccinations (OR of 1.17, CI 95%: 1.03–1.81). Participants with a higher mean score of having a positive attitude towards Covid-19 vaccination were more likely to have received a vaccination (OR of 1.12, CI 95%: 1.08–1.17). Ethnicity was also an influential variable, people with non-Fars ethnicity were less likely to be vaccinated than those of Fars ethnicity (OR of 0.33, CI 95%: 0.13–0.81). People with higher-than-average monthly income were more likely to report vaccination than those with low monthly incomes (OR of 1.27, CI 95%: 1.09–1.8). Also, participants reporting less access to vaccination centers had a lower chance of reporting having been vaccinated than those who reported high access to vaccination centers (OR of .17, CI 95%: .08-.36).ConclusionsCovid-19 vaccine uptake was found to be relatively high among people using illicit substances in this study. Higher levels of education, Fars ethnicity, higher income levels, having a positive attitude towards vaccination and access to vaccination centers were the most important predictors of Covid-19 vaccination in this study.

Self-Perception of Extroversion Increases with Social Media Use: A Social Media Induction Task.

Previous studies have examined the relationship between social media and extroversion, often relying on correlational designs. This study introduced a social media induction procedure to examine the relationship between social media and extroversion with an experimental design. In a procedure adapted from the personality research literature, participants used a written prompt to imagine themselves as a social media influencer and were then asked to use social media with this mindset. Participants' extroversion was measured before and after this activity using the Big Five Inventory. The results show that this social media induction procedure resulted in increased reports of extroversion after the task, and this post-task change was not predicted by other related social media factors (such as hours of social media use per day). These results complicate the assertion that differences in social media use between extroverts and introverts are due to its stronger appeal to extroverts.

Downregulation of PDIA3 inhibits gastric cancer cell growth through cell cycle regulation

ObjectiveProtein disulfide isomerase A3 (PDIA3) promotes the correct folding of newly synthesized glycoproteins in the endoplasmic reticulum. PDIA3 is overexpressed in most tumors, and it may become a biomarker of cancer prognosis and immunotherapy. Our study aims to detect the expression level of PDIA3 in gastric cancer (GC) and its association with GC development as wells as the underlying mechanisms. MethodsGC cell lines with PDIA3 knockdown by siRNA, CRISPR-cas9 sgRNAs or a pharmacological inhibitor of LOC14 were prepared and used. PDIA3 knockout GC cells were established by CRISPR-cas9-PDIA3 system. The proliferation, migration, invasion and cell cycle of GC cells were analyzed by cell counting kit-8 assay, wound healing assay, transwell assay and flow cytometry, respectively. Immunodeficient nude mice was used to evaluate the role of PDIA3 in tumor formation. Quantitative PCR and western blot were used for examining gene and protein expressions. RNA sequencing was performed to see the altered gene expression. ResultsThe expressions of PDIA3 in GC tissues and cells were increased significantly, and its expression was negatively correlated with the three-year survival rate of GC patients. Down-regulation of PDIA3 by siRNA, LOC14 or CRISPR-cas9 significantly inhibited proliferation, invasion and migration of GC cells TMK1 and AGS, with cell cycle arrested at G2/M phase. Meanwhile, decreased PDIA3 significantly inhibited growth of tumor xenograft in vivo. It was found that cyclin G1 (encoded by CCNG1 gene) expression was decreased by downregulation of PDIA3 in GC cells both in vitro and in vivo. In addition, protein levels of other cell cycle related factors including cyclin D1, CDK2, and CDK6 were also significantly decreased. Further study showed that STAT3 was associated with PDIA3-mediated cyclin G1 regulation. ConclusionPDIA3 plays an oncogenic role in GC. Our findings unfolded the functional role of PDIA3 in GC development and highlighted a novel target for cancer therapeutic strategy.