Related Behavioral Changes Research Articles

Topic tracking model for analyzing student-generated posts in SPOC discussion forums

Due to an overwhelming amount of student-generated forum posts in small private online courses (SPOCs), students and instructors find it time-consuming and challenging to effectively navigate and track valuable information, such as the evolution of topics, emotional and behavioral changes in relation to topics. For solving this problem, this study analyzed plenty of discussion posts using an improved dynamic topic model, Time Information-Emotion Behavior Model (TI-EBTM). Time, emotion, and behavior characteristics were incorporated into the topic modeling process, which allowed for an overview of automatic tracking and understanding of temporal topic changes in SPOC discussion forums. The experiment on data from 30 SPOC courses showed that TI-EBTM outperformed other dynamic topic models and was effective in extracting prominent topics over time. Furthermore, we conducted an in-depth temporal topic analysis to investigate the utility of TI-EBTM in a case study. The results of the case study demonstrated that our methodology and analysis shed light on students’ temporal focuses (i.e., the changes of topic intensity and topic content) and reflected the evolution of topics’ emotional and behavioral tendencies. For example, students tended to express more negative emotions toward the topic about the method of data query by initiating the conversation at the end of the semester. The analytical results can provide instructors with valuable insights into the development of course forums and enable them to fine-tune course forums to suit students’ requirements, which will subsequently be helpful in enhancing discussion interaction and students’ learning experience.

Promotion of Advance Care Planning Among Young Adults: A Pilot Study of Health Engagement Workshop Feasibility, Implementation, and Efficacy.

This pilot study set out to evaluate the feasibility and efficacy of an interactive, peer-led, health engagement workshop to improve confidence and comprehension related to advance care planning (ACP) among young adults. Secondarily, this study evaluated if such workshops could promote ACP related behavior changes within this population. This observational cohort study utilized a repeated measures, mixed-method design. Six hour-long, in-person workshops were conducted with undergraduate students during meetings of university student organizations. Participants were evaluated across 3 mixed-method surveys, evaluating confidence, knowledge, and behaviors related to ACP prior to participation, directly after, and during a 2-week follow-up. Workshop participation improved the average participant confidence and knowledge related to ACP as well as encouraged some participants to engage in discussions related to end-of-life care with friends and family. Alongside the impact of the workshops on knowledge and confidence, participants positively evaluated the design of the workshops through collected qualitative feedback. These results are encouraging in assessing this population's willingness to learn about end-of-life care planning. The tools developed and the corresponding results should be used for further exploration of engaging the young adult population in ACP to promote improved healthcare outcomes.

Perceived risk, behavior changes and Health-related outcomes during COVID-19 pandemic: Findings among adults with and without diabetes in China

AimsTo examine perceived infection risk of COVID-19 and the health and related behavior changes among people with diabetes, compared with people without diabetes, and to examine factors associated with self-reported health during the national quarantine period in China. MethodsThe 2020 China COVID-19 Survey is an anonymous 74-item survey administered via social media across China. A national sample of 10,545 adults in all 31 provinces in mainland China provided data on sociodemographic characteristics, awareness, attitudes towards COVID-19, lifestyle factors, and health outcomes during the quarantine. Regression models tested associations among study variables adjusting for covariates. ResultsAmong the 9,016 total participants (42.6% men and 57.4% women), 585 reported having diagnosed diabetes and 8,431 had no diabetes. Participants with diabetes perceived themselves to be at higher risk and were more worried about being infected with COVID-19 when compared to non-diabetic individuals (p < 0.001). During the COVID-19 pandemic, participants with diabetes were more likely to experience food and drug shortages and to increase their physical activity, compared to their counterparts. Among diabetic respondents, a high proportion of current smokers (74.1%) and drinkers (68.5%) reported increased amounts of smoking and drinking. People with diabetes were 11% less likely to report excellent or very good health. Having 150 min/week physical activity was positively associated with excellent or very good health (prevalence ratio, PR = 1.14, 95%CI 1.11–1.16). ConclusionsA high proportion of people with diabetes perceived risk of COVID-19 infection and increased their smoking and drinking during the pandemic.

Public Behavior Change, Perceptions, Depression, and Anxiety in Relation to the COVID-19 Outbreak.

BackgroundCOVID-19 has spread rapidly and internationally, which has elicited public panic and psychological problems. Public protective behaviors and perception play crucial roles in controlling the spread of illness and psychological status.MethodsWe conducted a cross-sectional online survey in the hardest-hit Hubei province and other areas in China affected by the COVID-19 outbreak. Questions about their basic information, the perception of the COVID-19 outbreak, recent preventive or avoidance behaviors, and self-reported mental health scales including the Patient Health Questionnaire and Self-Rating Anxiety Scale were included. Binary logistic regressions were used to investigate the association between personal variables/perceptions and psychological distress.ResultsA total of 6261 people were included in the analysis, with 3613 (57.7%) in Hubei province (1743 in Wuhan). The majority of people have adopted preventive and avoidance behaviors. People from Hubei, with contact history, and people who were infected or whose family members were infected had a much higher prevalence of depression and anxiety. Providing truthful and sufficient information, informing the public about the severity of the disease, and perceptions that the outbreak can be controlled by protective behaviors were associated with lower prevalence of depression and anxiety.ConclusionsAssessing the public response, perception, and psychological burden during the outbreak may help improve public health recommendations and deliver timely psychological intervention. Further research may focus on the psychological status of a specialized group to identify methods of delivery of better support based on public response and psychological demand.

MANAJEMEN MASJID PADA MASA PANDEMI COVID 19

The function of the mosque is very significant to human life, so that the mosque is very crowded with people, especially at certain times. The congregation that visits the mosque most is the male congregation. Meanwhile, men are a group that is vulnerable to covid-19. This condition is a potential distribution and transmission of covid-19. For this reason, there is a need for mosque management based on health transitions during the co-19 pandemic. The aim is to prevent and break the chain of distribution of covid-19. This management incorporates elements of social conception and behavior change in relation to health determinants. This management includes: first, physical management consisting of management, construction and physical maintenance ofmosques, maintenance of mosque cleanliness, management of mosque facilities. Second, functional management which includes the function of the mosque as a place of propaganda, a place of education, and a place for socialization and information about preventing the spread of covid-19. Keywords: Management, Mosque, Covid Pandemic 19.

The Effects of a Traditional Chinese Medication on Children with Attention-Deficit/Hyperactivity Disorder.

Objective: This feasibility study examined the effects of a particular Traditional Chinese Medicine (TCM) herbal formula on attention-deficit/hyperactivity disorder (ADHD) and related problem behaviors. Design: A total of 79 participants aging 6-12 years consumed a granulated TCM herbal formula twice daily over a period of 3 months and underwent assessments at months 0, 3, and 6. Changes in ADHD symptoms and related behaviors were measured using the ADHD rating scale-IV (ADHD-RS-IV), child behavior checklist (CBCL), children's global sssessment scale (CGAS), as well as the clinical global impressions-severity (CGI-S) and improvement (CGI-I) scales. Results: Repeated measures mixed model analyses revealed significant differences in scores across time on all ADHD-RS-IV and CBCL subscales as well as on the CGAS, CGI-S, and CGI-I scales. Pairwise comparisons between months 0 and 3 as well as months 0 and 6 indicated significant improvements in scores. Scores also did not differ significantly between months 3 and 6. The results may suggest that this particular TCM formula possesses potential therapeutic qualities in the treatment of ADHD. Furthermore, changes in ADHD symptoms generally appear to be stable 3 months after discontinuation. However, these findings could also be attributed to placebo effects as well as reporting biases. Conclusion: This particular TCM formula may prove to be a useful adjunctive treatment for children with ADHD, and randomized controlled trials need to be conducted to evaluate its efficacy.

Progressive impairment of learning and memory in adult zebrafish treated by Al2O3 nanoparticles when in embryos

Al2O3 Nanoparticles (Al2O3-NPs) have been widely used because of their unique physical and chemical properties, and Al2O3-NPs can be released into the environment directly or indirectly. Our previous research found that 13 nm Al2O3-NPs can induce neural cell death and autophagy in primarily cultured neural cells in vitro. The aim of this study was to determine where Al2O3-NPs at 13 nm particle size can cause neural cells in vivo and assess related behavioural changes and involved potential mechanisms. Zebrafish from embryo to adult were selected as animal models. Learning and memory as functional indicators of neural cells in zebrafish were measured during the development from embryo to adult. Our results indicate that Al2O3-NPs treatment in zebrafish embryos stages can cause the accumulation of aluminium content in zebrafish brain tissue, leading to progressive impaired neurodevelopmental behaviours and latent learning and memory performance. Additionally, oxidative stress and disruption of dopaminergic transmission in zebrafish brain tissues are correlated with the dose-dependent and age-dependent accumulation of aluminium content. Moreover, the number of neural cells in the telencephalon tissue treated with Al2O3-NPs significantly declined, and the ultramicroscopic morphology indicated profound autophagy alternations. The results suggest that Al2O3-NPs has dose-dependent and time-dependent progressive damage on learning and memory performance in adult zebrafish when treated in embryos. This is the first study of the effects of Al2O3-NPs on learning and memory during the development of zebrafish from embryo to adult.

Moderate-to-vigorous physical activity and processing speed: predicting adaptive change in ADHD levels and related impairments in preschoolers.

Preschool ADHD symptoms have predictive utility for later presence of ADHD diagnoses (Harvey, Youngwirth, Thakar, & Errazuriz, 2009, Journal of Consulting and Clinical Psychology, 77, 349; Lahey et al., 2004, American Journal of Psychiatry, 161(11), 2014), yet some level of inattention, hyperactivity, and impulsivity are present even in typically developing preschoolers. Physical activity (PA) is known to have a broad spectrum of positive effects on the brain in school-age typically developing children (Centers for Disease Control and Prevention, 2010, The association between school based physical activity, including physical education, and academic performance. Atlanta, GA: U.S. Department of Health and Human Services), including functions impaired by ADHD (Halperin, Berwid, & O'Neill, 2014, Child and Adolescent Psychiatric Clinics of North America, 23, 899), yet links between PA and ADHD levels and impairments have rarely been studied in either typically developing or at-risk preschool children. Importantly, impaired processing speed (PS), though not a symptom of ADHD, is a robust neuropsychological correlate (Willcutt & Bidwell, 2011, Treating attention deficit hyperactivity disorder: Assessment and intervention in developmental context. Kingston, NJ: Civic Research Institute) that may indicate additional risk for ADHD. Hence, we examined whether baseline PS moderates the association between preschoolers' PA, specifically moderate-to-vigorous PA (MVPA), and changes in ADHD levels and related behaviors. Eighty-five preschoolers (49.4% female; Mage =4.14, SDage =.64) were drawn from a larger study of the effects of the Kiddie Children and Teachers (CATs) on the Move PA program on school readiness. The sample was largely Head Start eligible (68.2%) and ethnically diverse. Hierarchical regressions were utilized to examine links between MVPA, averaged over a school year, and changes in inattention (IA), hyperactivity/impulsivity (HI), oppositional behaviors, moodiness, and peer functioning, and whether these associations varied based on baseline PS. Results indicated that for IA, HI, and peer functioning, higher amounts of MVPA were associated with greater adaptive change for those with lower (but not higher) levels of PS. Preschool MVPA may be a viable method of reducing ADHD levels and impairments for those with lower PS.

Using a Delphi study to clarify the landscape and core outcomes in environmental education

When leaders in a field agree upon core outcomes for the field, connection, reinforcement, and a shared vision of change are synergistically strengthened. Even when the field is dynamic, theoretically wide-ranging, and dispersed, consensus around core outcomes can help develop strategic research agendas and priorities. Environmental education (EE) is an inherently interdisciplinary field drawing on diverse theoretical foundations and epistemological orientations, resulting in a broad conceptual and empirical landscape. To better understand and situate this within EE, we pursued a modified Delphi study, with participation from 44 professionals and leaders active in North America. Over three Delphi rounds, the panel came to agreement on five core outcomes that focus the EE field: (1) environmentally related action and behavior change, (2) connecting people to nature, (3) improving environmental outcomes, (4) improving social/cultural outcomes, and (5) learning environmentally relevant skills and competencies. Reflecting those commonalities, we propose the following encapsulating statement to describe the field’s core outcomes: Environmental education works to move people to action for the tangible benefit of the environment and humanity. To realize these benefits, people must connect experientially with the environment, learn needed skills, and understand the complicated social and cultural connections between humanity and the natural environment.

Exploring Mechanisms of Action: Using a Testing Typology to Understand Intervention Performance in an HIV Self-Testing RCT in England and Wales.

SELPHI involves two interventions: A provides one HIV self-testing (HIVST) kit; B offers 3-monthly repeat HIVST kits if participants report ongoing risk. A logic model underpinned by the Behaviour Change Wheel informed the design of the intervention. SELPHI recruited 10,135 cis-men and trans people in England and Wales, all reporting anal sex with a man. This paper explores how the interventions were experienced and the mechanisms of action leading to impact for different groups of trial participants. In-depth interviews with 37 cis-men who have sex with men (MSM) were used to inductively categorise participants based on sexual and HIV testing histories. Themes relating to intervention experiences and impacts were mapped onto SELPHI-hypothesised intermediate outcomes to consider intervention impacts. Three groups were identified: ‘inexperienced testers’ engaged with SELPHI to overcome motivational and social and physical opportunity testing barriers. For ‘pro self-testers’, testing frequency was constrained by psychological and social barriers and lack of opportunity. ‘Opportunistic adopters’ engaged in HIVST for novelty and convenience. Perceived impacts for inexperienced testers were most closely aligned with the logic model, but for opportunistic adopters there was little evidence of impact. Distinctive groups were discernible with divergent intervention experiences. Using COM-B as a model for understanding behaviour change in relation to HIVST, our results indicate how HIVST interventions could be adapted to respond to different needs based on the target population’s demographic and behavioural features.

Development of the COVID-19 Phobia Scale: Validity and Reliability Study

Objective: It is suggested that the psychological and emotional reactions that occurred during the COVID-19 pandemic may lead to serious psychiatric disorders in the long run. This study aims to establish the validity and reliability of the COVID-19 Phobia Scale developed for measuring emotions and behaviors related to the COVID-19 pandemic. Methods: The sociodemographic form, COVID-19 Phobia Scale developed by the researchers, Hospital Anxiety and Depression Scale, and Health Anxiety Scale were applied in the study conducted through a web-based survey method. Results: An item-total analysis was performed as the first step of the study that was conducted with 1243 participants. The exploratory and confirmatory factor analyses revealed the final version of the COVID-19 Phobia Scale to comprise four subscales made up of 22 items, where the subscales were termed as worry, mood, reassurance seeking/precaution, and avoidance. The internal consistency of the COVID-19 Phobia Scale measured by the Cronbach's alpha coefficient was 0.84. A statistically significant positive correlation was found between the COVID-19 Phobia Scale and Hospital Anxiety and Depression Scale, Health Anxiety Scale. Conclusion: The COVID-19 Phobia Scale is a valid and reliable scale that can be used to measure emotions and related behavioral changes.

Antidepressant-like effects of β-caryophyllene on restraint plus stress-induced depression

Chronic stress is depressogenic by altering neurotrophic and neuroinflammatory environments of the organism. The endocannabinoid system controls cognitive and emotional responses related with stress through the interaction with endocannabinoid receptors. β-Caryophyllene (BCP) is a CB2 agonist that exhibited anti-inflammatory, analgesic effects but minimal psychoactive effects.To test if BCP exhibits antidepressant-like action, animals were chronically restrained with additional stressors for 28 days, and BCP (25, 50, 100 mg/kg) was intraperitoneally injected once a day during the stress inflicting period. Then despair related behaviors and hippocampal expression of neurotrophic, inflammatory and cannabinoid receptor levels were measured. To test the effect of BCP on long-term depression, field potentials were measured during the application of lipopolysaccharide and low frequency stimulation.In the tail suspension test and forced swim test, chronic stress-induced despair behaviors were reduced by BCP. Also BCP improved the stress-related changes in the hippocampal expression of COX-2, BDNF, and CB2 receptor expression. In organotypic hippocampal slices, BCP reduced the lipopolysaccharide-induced intensification of the long-term depression. In conclusion, BCP improved chronic stress related behavioral and biochemical changes. These results suggest that BCP may be effective in treating depression and stress related mental illnesses.

Implementation of a behavioral medicine approach in physiotherapy: a process evaluation of facilitation methods

BackgroundIn a quasi-experimental study, facilitation was used to support implementation of the behavioral medicine approach in physiotherapy. The facilitation consisted of an individually tailored multifaceted intervention including outreach visits, peer coaching, educational materials, individual goal-setting, video feedback, self-monitoring in a diary, manager support, and information leaflets to patients. A behavioral medicine approach implies a focus on health related behavior change. Clinical behavioral change was initiated but not maintained among the participating physiotherapists. To explain these findings, a deeper understanding of the implementation process is necessary. The aim was therefore to explore the impact mechanisms in the implementation of a behavioral medicine approach in physiotherapy by examining dose, reach, and participant experiences.MethodsAn explorative mixed-methods design was used as a part of a quasi-experimental trial. Twenty four physiotherapists working in primary health care were included in the quasi-experimental trial, and all physiotherapists in the experimental group (n = 15) were included in the current study. A facilitation intervention based mainly on social cognitive theory was tested during a 6-month period. Data were collected during and after the implementation period by self-reports of time allocation regarding participation in different implementation methods, documentation of individual goals, ranking of the most important implementation methods, and semi-structured interviews. Descriptive statistical methods and inductive content analysis were used.ResultsThe physiotherapists participated most frequently in the following implementation methods: outreach visits, peer coaching, educational materials, and individual goal-setting. They also considered these methods to be the most important for implementation, contributing to support for learning, practice, memory, emotions, self-management, and time management. However, time management support from the manager was lacking.ConclusionsThe findings indicate that different mechanisms govern the initiation and maintenance of clinical behavior change. The impact mechanisms for initiation of clinical behavior change refers to the use of externally initiated multiple methods, such as feedback on practice, time management, and extrinsic motivation. The lack of self-regulation capability, intrinsic motivation, and continued support after the implementation intervention period were interpreted as possible mechanisms for the failure of maintaining the behavioral change over time.

Hormonal regulation of pregnancy and related behavioral changes in rabbits

Hormonal regulation of rabbit pregnancy and related behavioral changes has been extensively studied for the last century. The present review aimed to summarize and clarify certain aspects of this period. The article consists of six chapters describing in sequence: maternal reproductive track, gestation, maternal cyclical phenomena, pregnancy and lactation, hormonal profile in pseudo-pregnancy, behavioral changes.

“If it ain’t broke don’t fix it”

A large part of preventing mastitis on dairy farms is achieved through the use of recommended milking practices (RMPs) that have been established and promoted by industry and milk quality organizations such as the National Mastitis Council and the Canadian Bovine Mastitis and Milk Quality Research Network. These practices include use of milking gloves, foremilk stripping, use of pre and post milking teat disinfectants, drying teats prior to milking, and use of automatic takeoffs on milking machines. However, many producers do not adopt these measures, or they only do so partially. This study aimed to explore the attitudes and perceptions of Ontario dairy farmers towards barriers for implementation of RMPs, and to investigate what motivates behavior change in relation to milking hygiene.

Reproductive, Maternal, Newborn, Child and Adolescent Health and related Behaviour Change Communication strategies in Bangladesh, Nepal and India: A narrative review

AimThe review was conducted to collate the evidence on the current status of “Reproductive, Maternal, Newborn, Child and Adolescent Health” (RMNCAH) and related Behaviour Change Communication (BCC) interventions in RMNCAH in South Asia, particularly Bangladesh, Nepal and India. MethodsLiterature search was conducted on multiple databases and websites in March 2019. The search was limited to English language publications. The data on health indicators for the eight South Asian countries are compared in the review. This review narratively summarises the key RMNCAH and related BCC initiatives undertaken in Bangladesh, Nepal and India. ResultsThe South Asian countries have achieved significant progress in socio-economic indicators related to health aligned with the Millennium Development Goals but face a number of challenges. There has been a significant development in financial incentives, programmes and policies framed for improving indicators of maternal and child health. Various RMNCAH initiatives which impact positively on maternal and child health indicators were identified. ConclusionBangladesh, Nepal and India have made substantial improvement in maternal and child health indicators but at a slower pace than other countries of South Asia, in relation to which consistent disparities still exist. There are a number of BCC initiatives targeting RMNCAH. The improvement in RMNCAH indicators can be attributed to multiple initiatives wherein the contribution of BCC is relevant, because of their wide community outreach and effectiveness among all age-groups.

A qualitative study of Ontario dairy farmer attitudes and perceptions toward implementing recommended milking practices

Recommended milking practices (RMP) are protective against mastitis. However, many producers do not adopt, or only partially adopt, these measures. This study aimed to explore the attitudes and perceptions of Ontario dairy farmers toward barriers to implementation of RMP and to investigate what motivates behavior change in relation to milking hygiene. Four focus groups with Ontario dairy producers were conducted, and verbatim transcripts were analyzed thematically. The main barriers to adoption of RMP were identified and categorized into 2 groups: intrinsic barriers and physical barriers. Intrinsic barriers included personal habits and convenience, not perceiving udder health as a priority on their farm, and lack of information. Physical barriers included employee training and compliance, convenience of implementing RMP, and time, money, and labor barriers. Producers used their bulk tank somatic cell count (SCC) as a measure of perceived severity of udder health problems on farm. Those with lower SCC were less likely to prioritize udder health compared with peers experiencing elevations in SCC. Lack of udder health problems translated for some producers into non-adoption of certain RMP, as they felt these practices were not needed unless a problem arose. Others felt motivated to implement more practices and work toward better udder health if such efforts translated into rewards for better-quality milk. Some producers perceived RMP as not meaningful or useful, seemingly due to a lack of education about the reasons behind RMP implementation. Understanding the importance of these practices is one key to implementing them. To overcome some of the intrinsic barriers, increased efforts in knowledge translation are needed, including efforts in retraining current practices, as well as in establishing best practices.

Cross-sectional changes in weight status and weight related behaviors among Australian children and Australian Indigenous children between 2010 and 2015.

BackgroundSince 2006 there has been substantial long-term investment in school-based child obesity prevention programs in New South Wales (Australia). Whether these programs have led to population level improvements in children’s weight status and weight-related behaviors are yet to be determined. The purpose of this study was to describe changes in children’s weight status and weight-related behaviors, including Indigenous children, who are at greater risk of poorer health outcomes than non-Indigenous children.MethodsRepresentative cross-sectional population surveys conducted in 2010 and 2015 among children age 5–16 years (n = 15,613). Objective measurements included height, weight, waist circumference, cardiorespiratory fitness, and fundamental movement skills. Indigenous status and indicators of weight-related behavior (i.e., diet, physical activity, school travel, screen-time) were measured by questionnaire with parents responding for children age <10 years and self-report by children age ≥10 years.ResultsThe prevalences of overweight/obesity, obesity and abdominal obesity were higher in 2015, than 2010, and higher among Indigenous than non-Indigenous children at both timepoints. There were some small positive changes towards healthier weight-related behaviors between surveys among all children, but many unhealthy weight-related behaviors remain highly prevalent. The magnitude of changes and the 2015 prevalences of weight-related behaviors were generally similar for Indigenous and non-Indigenous children.ConclusionsSchools play an important role in health promotion, but our findings suggest the current approaches need re-thinking. Upstream factors that shape weight-related behaviors such as the regulation of the food industry and food environment, urban, neighborhood and public transport planning must be including in solutions to ensure populations can eat healthily and be physical active.

Construction and effects of functionalized graphene oxide nonparticles-mediated RNA interference of hypoxia-inducible factor-1α gene

Objective To explore the effects of graphene oxide (GO)-polyethylene glycol (PEG)-folic acid (FA)-pyrenemethylamine hydrochloride (PyNH2)-mediated RNA interference (RNAi) of hypoxia-inducible factor-1α (HIF-1α) on the biological behaviors of human pancreatic cancer Patu8988 cells. Methods GO-PEG-FA-PyNH2 and RNAi targeting HIF-1α gene (GO-PEG-FA-PyNH2-HIF-1α-RNAi) was constructed. The expressions of HIF-1α and glucose transporter 1 (Glut-1) in Patu8988 cells were determined after knockdown of HIF-1α by RNAi. The invasive ability, the proliferation and the cell cycle of Patu8988 cells were investigated. The effect of HIF-1α knockdown on the uptake of 18F-fluorodeoxyglucose (FDG) in Patu8988 cells was also detected. Comparison of data was conducted by one-way analysis of variance and least significant difference t test. Results The GO-PEG-FA-PyNH2 was successfully constructed, and no cytotoxicity was found. Under the hypoxia or normoxia state, the mRNA and protein levels of HIF-1α and mRNA level of Glut-1 in cells transfected with GO-PEG-FA-PyNH2-HIF-1α-RNAi (study group) were lower than those in cells transfected with GO-PEG-FA-PyNH2(negative group) and cells transfected with Opti-minimal essential medium (Opti-MEM, control group; F=30.25-32.58, t=3.66-5.81, all P<0.05); the numbers of migrated cells in the study group were much lower than those in the negative group and the control group (F=38.63 and 41.35, t=20.51-35.25, all P<0.01); the cell proliferation in the study group was significantly lower than that in the negative group and the control group (F=35.19 and 38.11, t=15.11-22.19, all P<0.05). The proportions of G0/G1 cells in the study group were higher than those in the negative group and the control group (F=34.60 and 34.83, t=11.55-34.56, all P<0.05); the 18F-FDG uptake in the study group was lower than that in the negative group and control group (F=29.85 and 31.69, t=3.35-4.35, all P<0.05). Conclusion GO-PEG-FA-PyNH2-mediated HIF-1α RNAi inhibits the expression of HIF-1α in pancreatic cancer cells, leading to changes in related biological behaviors. Key words: Pancreatic neoplasms; Tumor cells, cultured; RNA, small interfering; Hypoxia-inducible factor 1, alpha subunit; Graphene oxide; Polyethylene glycols; Folic acid; 1-Pyrenemethylamine

Impact of Hepatitis C treatment on behavioural change in relation to drug use in people who inject drugs: A systematic review

Background: A systematic review was conducted to determine the impact of Hepatitis C (HCV) treatment on substance use behaviour in people who inject drugs (PWID).Methods: A search for peer reviewed journal articles from 1991 to present day was conducted using the following databases: PubMed, EMBASE, CINAHL and PsycINFO. Studies were appraised against the following inclusion criteria: recruitment of PWID for HCV treatment (either interferon alpha or direct acting antivirals based); measurement of behavioural change in relation to drug use; studies published in English.Results: Five studies investigating the impact of HCV treatment on behavioural change in relation to drug use amongst PWID were identified. Studies investigated the impact of HCV treatment on past month injecting drug use (four studies), injecting frequency (two studies), needle and syringe borrowing (two studies) and injecting equipment sharing (three studies). Three of the four studies assessing impact of treatment on past month injecting frequency found treatment significantly reduced the odds of participants reporting past month injecting at follow up. One study found that there was significant reduction in weekly injecting frequency between enrolment, treatment and follow up. No association was found between treatment engagement and needle and syringe borrowing. Two out of three studies reported a significant decrease in injecting equipment sharing between enrolment, treatment and follow up.Conclusions: Comparison and synthesis of results was challenging due to heterogeneity between studies. Moreover, four out of the five selected studies were conducted during the interferon era of treatment, possibly limiting the generalisability of the current review’s results to the new DAA treatment era. However, it is likely that engaging in treatment has a positive impact upon patients’ injecting drug use and injection equipment sharing behaviour. This raises the possibility that this may be an opportune time for further harm reduction measures.