Rejuvenation Program Research Articles

Participation of Planters in the Implementation of Community Palm Oil Rejuvenation (PSR) in Makmur Village, Pangkalan Kerinci District, Pelalawan Regency

The research was carried out in the village of Makmur, Kerinci base district of Pelalawan. This research aims to analyze any factors that are significantly related to the participation of farmers in the implementation of the People's Sawit Rejuvenation (PSR) program in the village of Makmur, Kerinci Base district of Pelalawan. The sampling method used in this study is using simple random samplings. The data analysis used in this study is the Spearman Rank correlation test to measure the degree or intensity of the relationship between two variables, the free variable and the bound variable. The results of the research showed that there was a significant relationship between the factors of land size, income, motivation, experience, capital assistance and availability of means of production with the participation of farmers in the implementation of the People's Sawit Rejuvenation (PSR) program in the village of Makmur, Kerinci district. There is a need for increased participation of farmers through agricultural development to improve the capacity in palm coconut rejuvenation activities in the village of Makmur at the Kerinci Base district of Pelalawan, so that it can improve the value of palm farmers' use and it is necessary to monitor and evaluate the accompanying forces continuously so that there are no deviations in the program, so the People's Sawit Rejuvenate (PSR) program can continue for other farmers.

Increasing Agricultural Productivity in the Arabica Coffee Replanting Programme through a Gender Responsive Approach in Toraja District

Tana Toraja, Indonesia, is renowned for its "Toraja coffee," a highly sought-after specialty coffee. To meet the growing global demand, Toraja coffee production needs to increase. However, traditional gender roles often limit participation in coffee farm management. This research explores how integrating the roles of both men and women farmers can improve coffee production and farm management in Tana Toraja. A qualitative method with a phenomenological approach. Primary data sources are male and female farmers, and secondary data is supporting data from relevant agencies in Tana Toraja Regency, Indonesia. Data analysis techniques are used in the form of collection (observation, interviews, questionnaire filling), reduction (summarizing data), data presentation, and data verification (temporary conclusions). In the management of Arabica coffee, male farmers are involved 37.14 percent (less involved), female farmers 40.48 percent (less involved), the involvement of male and female farmers together, and alternately 77.62 percent (fully involved). The coffee rejuvenation program received a response of 45.00 percent (quite responsive) from male farmers; female farmers responded 46.66 (quite responsive), and the reaction of male and female farmers together and alternately was 91.67 percent (fully responsive). This research found that a gender-responsive approach, where both men and women farmers are fully involved in coffee farm management and rejuvenation programs, can significantly increase productivity and sustainability in Tana Toraja's smallholder coffee plantations. By integrating the roles of both genders, the research suggests that coffee farms can benefit from a wider range of skills, knowledge, and perspectives, ultimately leading to a more successful and sustainable coffee industry in Tana Toraja.

Development of an Accelerated Model for ISPO Certification in Independent Palm Oil Plantations

The Indonesian government has committed to implementing sustainable palm oil plantation development. This commitment goes through some regulations that require smallholder plantations (plasma and independent) to have Indonesian Sustainable Palm Oil Certification (ISPO certified). Even though the ISPO certification obligation has been in effect since 2020, until mid-2023, there are still very few ISPO-certified smallholder oil palm plantations. This research aims to develop a model for accelerating ISPO certification for independent oil palm plantations as the largest part of smallholder oil palm plantations. The ISPO Certification acceleration model can be implemented by synergizing the Smallholders’ Palm Oil Rejuvenation Program (PSR) – ISPO program, which is coordinated from the central to provincial and district levels. This acceleration model can carry out three roles simultaneously, namely assisting PSR participants who are also ISPO certified, empowering large private plantations (PBS/large national plantations (PBN)) to contribute to the success of the PSR/ISPO program, and focusing more on helping farmers who are not yet included in the PSR program and PBS/PBN for ISPO certification. Keywords: model, ISPO certification, oil palm, independent smallholders

Legal Study of the Legality of the Palm Oil Rejuvenation Program Regarding the Completeness and Correctness of the Proposal Documents Regulated by Ministerial Regulation No. 19 Of 2023 Amendment to Ministerial Regulation No. 3 Of 2022

Legal Study of the Legality of the Palm Oil Rejuvenation Program Regarding the Completeness and Correctness of the Proposal Documents Regulated by Ministerial Regulation No. 19 Of 2023 Amendment to Ministerial Regulation No. 3 Of 2022

Strategi Peremajaan Sawit Rakyat dan Pengembangan Usaha KUD Sumber Makmur Menggunakan Pendekatan Business Model Canvas

KUD Sumber Makmur is one of the Village Unit Cooperatives located in Pelalawan Regency, Riau. As a form of sustainable palm oil management, cooperative members' oil palm lands that have reached economic maturity need to be replanted. Apart from that, managing KUD cooperatives requires a cooperative business development strategy model. This research aims to identify strategies for rejuvenating smallholder palm oil and business development for KUD Sumber Makmur using the Business Model Canvas approach. The research was conducted in September 2023 at KUD Sumber Makmur, Pelalawan Regency, Riau. The data collection methods in this research are primary data and secondary data. The data analysis used in this research uses a qualitative descriptive type. The strategy for rejuvenating smallholder oil palms at KUD Sumber Makmur consists of the data collection stage and the implementation stage of the smallholder oil palm rejuvenation program. The business development strategy at KUD Sumber Makmur is: The Key Partner of the cooperative is PT Asian Agri, Key Activities of the cooperative consist of a savings and loan unit, agricultural production facilities unit, FFB Transportation unit, and FFB Sales unit, Value Proposition consists of cooperative FFB Quality and Palm Oil Certification, Customer relationships consist of efforts to maintain the quality of FFB and establish good communication with offtakers. Customer segments consist of off-taker companies (PT Asian Agri) and BPDPKS. Key resources consist of partner farmers' land, cooperative buildings, and transportation equipment. Channels consist of Direct and Contract methods, Cost Structure consists of production costs and operational costs, Revenue streams consist of FFB Sales

Chromatin-associated cohesin turns over extensively and forms new cohesive linkages in Drosophila oocytes during meiotic prophase

In dividing cells, accurate chromosome segregation depends on sister chromatid cohesion, protein linkages that are established during DNA replication. Faithful chromosome segregation in oocytes requires that cohesion, first established in S phase, remain intact for days to decades, depending on the organism. Premature loss of meiotic cohesion in oocytes leads to the production of aneuploid gametes and contributes to the increased incidence of meiotic segregation errors as women age (maternal age effect). The prevailing model is that cohesive linkages do not turn over in mammalian oocytes. However, we have previously reported that cohesion-related defects arise in Drosophila oocytes when individual cohesin subunits or cohesin regulators are knocked down after meiotic S phase. Here, we use two strategies to express a tagged cohesin subunit exclusively during mid-prophase in Drosophila oocytes and demonstrate that newly expressed cohesin is used to form de novo linkages after meiotic S phase. Cohesin along the arms of oocyte chromosomes appears to completely turn over within a 2-day window during prophase, whereas replacement is less extensive at centromeres. Unlike S-phase cohesion establishment, the formation of new cohesive linkages during meiotic prophase does not require acetylation of conserved lysines within the Smc3 head. Our findings indicate that maintenance of cohesion between S phase and chromosome segregation in Drosophila oocytes requires an active cohesion rejuvenation program that generates new cohesive linkages during meiotic prophase.

An RNAi screen to identify proteins required for cohesion rejuvenation during meiotic prophase in Drosophila oocytes.

Accurate chromosome segregation during meiosis requires the maintenance of sister chromatid cohesion, initially established during premeiotic S phase. In human oocytes, DNA replication and cohesion establishment occur decades before chromosome segregation and deterioration of meiotic cohesion is one factor that leads to increased segregation errors as women age. Our previous work led us to propose that a cohesion rejuvenation program operates to establish new cohesive linkages during meiotic prophase in Drosophila oocytes and depends on the cohesin loader Nipped-B and the cohesion establishment factor Eco. In support of this model, we recently demonstrated that chromosome-associated cohesin turns over extensively during meiotic prophase and failure to load cohesin onto chromosomes after premeiotic S phase results in arm cohesion defects in Drosophila oocytes. To identify proteins required for prophase cohesion rejuvenation but not S phase establishment, we conducted a Gal4-UAS inducible RNAi screen that utilized two distinct germline drivers. Using this strategy, we identified 29 gene products for which hairpin expression during meiotic prophase, but not premeiotic S phase, significantly increased segregation errors. Prophase knockdown of Brahma or Pumilio, two positives with functional links to the cohesin loader, caused a significant elevation in the missegregation of recombinant homologs, a phenotype consistent with premature loss of arm cohesion. Moreover, fluorescence in situ hybridization confirmed that Brahma, Pumilio, and Nipped-B are required during meiotic prophase for the maintenance of arm cohesion. Our data support the model that Brahma and Pumilio regulate Nipped-B-dependent cohesin loading during rejuvenation. Future analyses will better define the mechanism(s) that govern meiotic cohesion rejuvenation and whether additional prophase-specific positives function in this process.

The Implementation Process of the People's Palm Oil Rejuvenation Program (PSR) in Sungai Melayu Sub-District, Ketapang Regency

The residents of Sungai Melayu Village mostly make their living as oil palm farmers using old and unfit planting conditions. This research aims to analyze the implementation process of the People's Palm Oil Rejuvenation (PSR) Policy program in Sungai Melayu District, Ketapang Regency. The research method used is a qualitative descriptive approach as sources of information include the Head of Service, Head of Division, Sub-District Head, community leaders, and palm oil heads. The results of this study indicate that the PSR. The program Implementation Process is not yet optimal because the institutions involved still need to be stronger in synergy. In addition, funding and infrastructure support are lacking. The interpretation of the program that was rolled out was poorly understood by the farmers due to the lack of massive and programmed socialization.

Electrospun Nanofibers Encapsulated with Natural Products: A Novel Strategy to Counteract Skin Aging.

The skin is the primary tissue affected by wounds and aging, significantly impacting its protective function. Natural products are widely used in cosmetics, representing a new approach to preventing age-related damage. Nanomedicine combines nanotechnology and traditional treatments to create innovative drugs. The main targets of nanotechnological approaches are wound healing, regeneration, and rejuvenation of skin tissue. The skin barrier is not easily permeable, and the creation of modern nanodevices is a way to improve the passive penetration of substances. In this study, Helichrysum italicum oil (HO) was combined with different types of electrospun nanofibers to study their protective activity on the skin and to evaluate their future application for topical treatments. In the present research, we used biodegradable polymers, including polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), which were characterized by a scanning electron microscope (SEM). All results show a positive trend in cell proliferation and viability of human skin stem cells (SSCs) and BJ fibroblasts pre-treated with combined nanofibers and then exposed to UV stress. Gene expression analysis revealed the activation of a molecular rejuvenation program in SSCs treated with functionalized nanofibers before UV exposure. Understanding the mechanisms involved in skin changes during aging allows for the future application of nanomaterials combined with HO directly to the patients.

PERJANJIAN POLA KEMITRAAN DALAM PELAKSANAAN PEREMAJAAN TANAMAN KELAPA SAWIT

One effort to increase the productivity of oil palm plants is to carry out a replanting program. In implementing the oil palm plant rejuvenation program, most of the planters come from low-educated communities who lack knowledge regarding what and how partnership patterns are. It is the government's authority to regulate cooperative relations between plantation companies and farmers. This collaboration is carried out considering that planters may not be capable enough to work on rejuvenating their oil palm plantations, whereas if oil palm trees that are over 20-25 years old are not rejuvenated, they will no longer be productive and will produce little fruit. This collaboration is carried out by plantation companies as the core and plasma farmers/planters. In implementing this replanting program, there are several models of partnership patterns, one of which is the Intiplasm partnership pattern which is the most popular in implementing this program. The method used in this research is Normative Juridical with the research approach used is qualitative by examining statutory documents and comparing them with conditions in the field. The research results showed that the implementation of Minister of Agriculture Decree Number 940/KPTS/OT.210/10/97 concerning Guidelines for Agricultural Business Partnerships in the Implementation of Oil Palm Plant Rejuvenation has been carried out well, as evidenced by the existence of several regions in Indonesia that have successfully and completed following replanting using the intiplasma partnership pattern. This type of partnership pattern in implementing replanting is very important, because it can help people who do not have funds to continue participating in the replanting program. Then the form of legal protection obtained by farmers if a dispute occurs is through mediation and efforts to resolve disputes outside of court. Then, if no bright spot is found, the problem can then be resolved in court with all the consequences. Keywords: Palm Oil; Rejuvenation; Partnership Pattern.

Peran Koperasi Unit Desa (KUD) Marga Bhakti dalam Program Peremajaan Sawit Rakyat (PSR) di Desa Bagan Bhakti Kecamatan Balai Jaya Kabupaten Rokan Hilir

The rejuvenation of people's oil palm is one of the national strategic programs as an effort by the government to increase the productivity of oil palm plantations, by maintaining the area of land, so that oil palm plantations can be used optimally. To deal with the problem of palm oil that is no longer productive, the government created this people's oil palm rejuvenation program. The government gave instructions to all groups, both local government and the community, to be active and participate in the program, including the Balai Jaya sub-district, which took part in implementing the program. The Bagan Bhakti Village Government and also the Marga Bhakti Village Unit Cooperative are working together in running the program. The main problem is that the palm oil in Bagan Bhakti village is no longer productive which results in a decrease in income so that it is necessary to replant oil palm so that the economy in Bagan Bhakti Village is improving again. The purpose of this study was to determine the role of the Marga Bhakti Village Unit Cooperative and to find out what are the inhibiting factors in the people's oil palm rejuvenation program. This study uses the theory of Titik Sartika Pramono with indicators, building and developing the economic potential of members, improving the quality of human life, strengthening the people's economy, and creating and developing the country's economy. This study uses a qualitative research method with a descriptive approach, the data needed are primary data and secondary data through purposive sampling data collection techniques used, namely literature study, interviews, documentation, and observation. The results of the study indicate that the implementation of the program has been going well, there are only a few deficiencies in the program that have not been fulfilled. The inhibiting factor is the Marga Bhakti Village Unit Cooperative which has not been able to make the latest program for members who do not take part in the program to continue to support their income so that they remain good.
 

Issues and Strategies for Accelerating the Implementation of Sustainable Smallholder Oil Palm Rejuvenation Program in Indonesia

This research aims to analyze various literature that discusses problems and strategies for accelerating the realization of the national sustainable smallholder oil palm rejuvenation (PSR) program. The primary method used in this research is a literature study (desk study). The results of the study show that there are several strategies that the government must implement to accelerate the realization of the sustainable PSR program in Indonesia. The strategies in question include mandatory ISPO certification in 2025 (Indonesian Sustainable Palm Oil) for smallholder oil palm plantations, increasing the resource capacity of smallholder oil palm growers in implementing good agricultural practices (GAPs), providing access to smallholder oil palm growers to production inputs, resolving legality issues. Smallholders' land, encouraging land productivity and strengthening smallholders in fulfilling ISPO certification requirements, strengthening the institution of smallholders as a forum for smallholders to access government programs, and encouraging entry into the palm oil (CPO) processing sector which is an industrial raw material, and the government and related parties signed PSR cooperation through partnerships.

Macronutrient Contents and Yield of Cocoa Resulting from Two Different Rejuvenation Techniques

Cocoa (<em>Theobroma cacao </em>L.) is one of Indonesia’s leading and important export commodities. The cocoa plant rejuvenation techniques aim to increase cocoa production and quality. Ring budding and side grafting can rejuvenate old and unproductive cocoa plants. Therefore, this research was conducted with objectives: 1) to record macronutrient contents, yield components and yields of three cocoa clones, KKM 22, RCC 70 and RCC 71, rejuvenated with ring budding and side grafting, 2) to determine the best techniques in rejuvenation program for old cocoa stand based on nutrient contents, yield components, and yields indicators of three cocoa clones. This research was carried out from November 2020 to January 2021 at Cocoa Plantation, North Segayung Production Unit, Pagilaran Company. The results showed that ring-budded plants had the highest N nutrients content in leaves, stem organic-C production capacity, pods number per stand per year, dry weight bean per stand per year and dry weight bean per hectare per year, which was significantly better than side grafted plants. The macronutrient of total parts (leaves, stem, and root) trend was leaves > stem > root. The trend of macronutrients in cocoa were organic-C > N > Ca > K > Mg > P. Based on the macronutrient content of cocoa tissue, KKM 22 have a higher content of organic-C, N, P, K, Ca, and Mg compared to RCC 71 and RCC 70. The budding ring technique was the best in the rejuvenation program for old cocoa stands based on nutrient contents, yield components, and yield indicators of three cocoa clones.

ANALYSIS OF PARTICIPATION OF MELABUN PERMAI GAPOKTAN MEMBERS TO FOLLOW THE PEOPLE'S OIL PALM REJUVENATION (PSR) PROGRAM IN BANGKA CENTRAL DISTRICT

The People's Oil Palm Rejuvenation Program (PSR) is a National Strategic Program as an effort by the Government to increase the productivity of oil palm plantations. One important factor that determines the success of replanting activities is the participation of farmers, where without the participation of farmers, rejuvenation activities will not be successful, due to the role of farmers as the main actors in agricultural development programs. The purpose of this study was to determine the level of farmer participation and the factors that are significantly related to the implementation of People's Oil Palm Rejuvenation (PSR) in Sungaiselan District, Central Bangka Regency. The study of Gapoktan Melabun Permai in Sungaiselan Subdistrict, Central Bangka Regency was carried out from May to June 2023. The sample in this study was Gapoktan Melabun Permai, Sungaiselan District in Central Bangka Regency, totaling 59, the sampling technique of this study was non-probability sampling using consecutive sampling method, data collection used a questionnaire that had been tested for validity and reliability. Methods of data analysis to determine the participation of Gapoktan Melabun Permai in PSR using multiple linear regression analysis. The results of the study found that the participation of Gapoktan Melabun Permai in the implementation of the People's Palm Oil Rejuvenation (PSR) program in Sungaiselan District, Central Bangka Regency, was in the very high category with a percentage of 91.58%. After conducting multiple linear regression analysis, there is a significant relationship between the factors of land area, motivation,

Impact of Climate Change on Gravity Flow Drinking Water Resource in the Upper Kosi Watershed, Lesser Himalaya

In the age of climate change, the hydrological cycle is the most sensitive cycle which is being adversely affected. The natural water sources such as natural springs, rivulets, rivers etc of non-glacial fed watershed in Uttarakhand are drying and dying steadily disturbing society’s economy, and environment and creating threat on livelihood and ecosystem of the region. The basic goal of the current paper is to evaluate the impact of climate change on the gravity flow drinking water resource of the study area, Lesser Himalaya, Uttarakhand. For this objective, the hydrometeorological data is analyzed in this paper. The meteorological data, i.e., mean temperature (y = 0.059x + (-) 102.07) and average annual rainfall (y = 2.759x + (-) 4546.41) shows an increasing trend while the hydrological data shows a decreasing trend over the time. The study shows that in spite of an increasing trend in the annual rainfall pattern, the groundwater resource has been depleted significantly in the study area caused by change in rainfall rhythm which has disturbed the water recharging capacity of soil into the aquifers and the baseflow discharge of the gravity flow sources. Due to all these changes, the baseflow water discharge capacity of gravity flow sources have perturbed badly which is imposing threat on drinking water supply in the villages of the study area. At present, there are 93 gravity flow drinking water schemes in the study area. On an average, the baseflow water discharge of these schemes at their establishment year was recorded 40.11 l/m which has decreased to 23.46 l/m in 2018. Thus, on an average, about 16.65 l/m baseflow water discharge capacity of each scheme has been decreased since their establishment year. These data shows that how alarmingly the baseflow water discharge capacity at the sources of gravity flow schemes is depleting in the study area. Therefore, the study suggests a water source rejuvenation program for the sustenance of water sources, livelihood and ecosystem of the region.

Growth response of not-ready-to-distribute tea (Camellia sinensis (L) o. Kuntze) seedlings due to application of biofertilizer at various concentrations and intervals

The plantation rejuvenation program makes the need for ready-to-distribute tea seedlings getting higher. Meanwhile, the nursery's seedlings are mostly not ready to distribute. This study aimed to determine the response of not-ready-distribute tea seedlings due to the application of biofertilizers at various concentrations and intervals. This experiment was carried out at Gambung Tea and Quinine Research Center Nursery, conducted from December 2021 to February 2022 at an altitude of 1,350 meters above sea level (asl). The experimental method used was a randomized block design with eight treatment combinations, namely: control (no fertilizer); urea fertilizer every two weeks; Biofertilizer 5 mL L-1 + interval once a week; Biofertilizer 5 mL L-1 + interval every two weeks; Biofertilizer 10 mL L-1 + break once a week; Biofertilizer 10 mL L-1 + interval every two weeks; Biofertilizer 15 mL L-1 + interval once a week; Biofertilizer 15 mL L-1 + interval every two weeks, all repeated four times. The experimental results showed that treatment of biological fertilizers influenced the parameter number of leaves and chlorophyll index. The application of biofertilizer with a concentration of 15 mL L-1 + interval of 2 weeks greatly influenced the parameters of leaf number and chlorophyll content index.

Peran Pemerintah dalam Optimalisasi Pelaksanaan Peremajaan Tanaman Kelapa Sawit

Palm oil is one of the leading agricultural sectors which has an important role in Indonesia. In addition to being able to absorb new workers, the oil palm plantation business can also contribute to state revenues from crude palm oil (CPO) export levies, the demand for which is increasing every year. However, in recent years the level of productivity of oil palm plantations has decreased. This is due to the age of the plants that are getting older and not using superior and certified seeds at the start of planting. With this low level of productivity, it is necessary to carry out rejuvenation of oil palm plants. The target of this rejuvenation program is to be able to use both superior and certified seeds in order to increase plant productivity. This research uses normative juridical research methods, namely research conducted by researching and analyzing literature or secondary data only. The approach method is descriptive and qualitative research methods. The results showed that the government played an active role in implementing the oil palm rejuvenation program. This is evident from the many regulations made by the government or other stakeholders to support the smooth running of this program. In addition, as an effort to provide support in increasing palm oil productivity, especially in preparing funds for rejuvenation programs, the government has established the Oil Palm Plantation Fund (DPKS).

The relationship between farmer participation and the effectiveness of the people's palm oil rejuvenation program (PSR) in Tebo District, Jambi Province, Indonesia

The aims of this research are: (1) to analyze the level of participation of farmers in the People's Oil Palm Rejuvenation Program (PSR), (2) to analyze the effectiveness of the People's Oil Palm Rejuvenation program, and (3) to analyze the relationship between the level of participation and the effectiveness of the PSR program. The research was conducted in Tebo District, particularly in the Rimbo Ulu and Muara Tabir Districts. with the survey method. Sampling technique with Random Cluster Sampling of 182 farmers, and obtained a sample of 65 respondents consisting of 44 farmers in Muara Tabir District and 21 respondents in Rimbo Ulu District. Data were analyzed descriptively and Rank Spearman correlation test.The results showed that the level of farmer participation in Muara Tabir and Rimbo Ulu Districts was high with an index of 74.03% for Muara Tabir District and 74.15% for Rimbo Ulu District. The effectiveness of the PSR program is in the high category with an achievement of 79.73% for Rimbo Ulu District, and for Muara Tabir District an achievement of 75.11%. The relationship between farmer participation and the effectiveness of the PSR program in the two sub-districts shows no significant correlation, with a value of Rs = 0.243, and Rs = 0.445 , for Muara Tabir and for Rimbo Ulu, respectively. The direction of the relationship is positive, which means that the higher the level of farmer participation, the more the goals of the people's oil palm rejuvenation program are achieved.

Sosialisasi Program Sawit Rakyat Dalam Rangka Peningkatan Ekonomi Rakyat Kabupaten Nagan Raya Provinsi Aceh

This article aims to discuss the socialization of the people's palm oil program in the context of improving the people's economy in Nagan Raya Regency, Aceh Province. The government's focus on the oil palm rejuvenation program is supported by a replanting subsidy policy that uses funds from the Oil Palm Plantation Management Agency (BPDPKS). The purpose of the socialization activity is to provide information about the oil palm rejuvenation program and to arouse community enthusiasm for oil palm cultivation in Nagan Raya Regency in order to improve the welfare of oil palm farmers. The socialization activity explained in detail and clearly how best to carry out oil palm rejuvenation, explained the advantages and disadvantages of oil palm rejuvenation techniques, as well as provided information about the requirements and stages of oil palm rejuvenation activities, and helped increase access to groups receiving funds from the Oil Palm Plantation Fund Management Agency (BPDPKS).

The effect of 2,4-D, BA and Thidiazuron on somatic embryo induction of liberica coffee of Tungkal Composite from Jambi

The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status of the plants are old and showing diseased attacked. To support the rejuvenation, the provision of seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed to study the effect of 2,4-D, BA and TDZ on callus formation and embryo induction from liberica coffee leaf explants. Explants used were leaf sections with or without veins. Explants were grown on Murashige Skoog (MS) basal medium with 2,4-D + BA and 2,4-D + TDZ. A completely randomized design was employed in the trial, and each experimental unit was replicated three times. Results showed that callus could be formed on both types of explants, but leaf explants with veins could produce callus faster. The use of BA in medium could induce callus formation faster than TDZ. The higher the concentration of 2,4-D, BA and TDZ, the slower callus initiation. The addition of TDZ in the medium resulted in mostly callus, while callus from medium with BA showed a friable structure. Globular somatic embryos were formed in all treatments with varying amounts. TDZ can induce more globular somatic embryo than BA.