In the present study, the samples of clarified milk fat (CMF) of yak species (Arunachali yak and yak-cow hybrid) were collected and analysed for physicochemical parameters, colour values, fatty acid composition and triglyceride profile, and FT-IR characteristics. The obtained results showed that CMF contains a variable amount of moisture (0.25%–20.28%), free fatty acid (0.12-2.03% oleic acid), peroxide values (0.30-1.20 meq.O2/kg fat), Reichert Meissl value (19.06-23.54) and Butyro-Refractrometer reading (43.03-44.47). The colour value (L*, a*, b*) varied with altitude level and region significantly (p<0.05). The regional variations in triglycerides and fatty acid profile of CMF were also found significantly (p<0.05). The preliminary data of FT-IR spectra of all samples showed almost similar functional groups but slight difference in the absorbance as well as peaks in the finger print region.