Pressure Regulation Research Articles

A mechanohydraulic model supports a role for plasmodesmata in cotton fiber elongation.

Plant cell growth depends on turgor pressure, the cell hydrodynamic pressure, which drives expansion of the extracellular matrix (the cell wall). Turgor pressure regulation depends on several physical, chemical, and biological factors, including vacuolar invertases, which modulate osmotic pressure of the cell, aquaporins, which determine the permeability of the plasma membrane to water, cell wall remodeling factors, which determine cell wall extensibility (inverse of effective viscosity), and plasmodesmata, which are membrane-lined channels that allow free movement of water and solutes between cytoplasms of neighboring cells, like gap junctions in animals. Plasmodesmata permeability varies during plant development and experimental studies have correlated changes in the permeability of plasmodesmal channels to turgor pressure variations. Here, we study the role of plasmodesmal permeability in cotton fiber growth, a type of cell that increases in length by at least three orders of magnitude in a few weeks. We incorporated plasmodesma-dependent movement of water and solutes into a classical model of plant cell expansion. We performed a sensitivity analysis to changes in values of model parameters and found that plasmodesmal permeability is among the most important factors for building up turgor pressure and expanding cotton fibers. Moreover, we found that nonmonotonic behaviors of turgor pressure that have been reported previously in cotton fibers cannot be recovered without accounting for dynamic changes of the parameters used in the model. Altogether, our results suggest an important role for plasmodesmal permeability in the regulation of turgor pressure.

Second Harmonic Generation in β-K2TeW3O12: An Acentric Crystal Designed from Centric Phase via Pressure Modulation.

The latent value of nonlinear optical (NLO) crystals applied in solid-state laser equipment necessitates the development of applicable strategies for constructing noncentrosymmetric (NCS) crystals. By modulating the synthetic temperature and pressure to achieve the rearrangement of [TeO3]2- groups, a new NCS tellurium tungstate, β-K2TeW3O12 (β-KTW), with a strong second harmonic generation (SHG) response was synthesized based on its centrosymmetric polymorphic phase α-K2TeW3O12 (α-KTW). Computational calculation reveals that the large SHG response of β-KTW (15 × KH2PO4@1064 and 1.5 × KTiOPO4@1950 nm) could be attributed to the uniform arrangement of the NLO-active [TeO3]2- and [WO6]6- groups. β-KTW also exhibits enlarged birefringence (0.196@1064 nm) and a high laser damage threshold (42.3 MW cm-2), showing great potential as a nonlinear crystalline material. This work also provides a new route for the construction of NLO crystals based on centric structure, i.e., reverse pressure regulation.

Hydropower capacity factors trending down in the United States

The United States hydropower fleet has faced increasing environmental and regulatory pressures over the last half century, potentially constraining total generation. Here we show that annual capacity factor has declined at four fifths of United States hydropower plants since 1980, with two thirds of decreasing trends significant at p < 0.05. Results are based on an analysis of annual energy generation totals and nameplate capacities for 610 plants (>5 megawatt), representing 87% of total conventional hydropower capacity in the United States. On aggregate, changes in capacity factor imply a fleetwide, cumulative generation decrease of 23% since 1980 before factoring in capacity upgrades—akin to retiring a Hoover Dam once every two to three years. Changes in water availability explain energy decline in only 21% of plants, highlighting the importance of non-climatic drivers of generation, including deterioration of plant equipment as well as changes to dam operations in support of nonpower objectives.

Effects of gamma, electron beam, and X-ray irradiation on the plastic components of a universal bone cement mixer medical device

Due to market and regulatory pressures, many healthcare manufacturers are considering alternatives to cobalt-60 gamma radiation, including accelerator-based electron beam (E-beam) and X-ray radiation for product sterilization. In this work, the effects of irradiation on a representative medical product, comprised of eight distinct polymer materials, were directly compared for three radiation technologies – cobalt-60 gamma, E-beam, and X-ray – at four dose levels (15, 25, 50, and 70 kGy). The objective was to determine how radiation effects (deleterious or beneficial) are influenced by source and dose level, with the specific goal of determining whether E-beam radiation and/or X-ray radiation could be viable alternatives to gamma radiation for device sterilization. The specific product considered is a single-use medical device for orthopedic surgery, the Stryker Instruments MixeVac III bone cement mixer, which is currently sterilized using gamma radiation from cobalt-60 sources. Following ASTM International standards and input from the manufacturer, we characterized the effects of the three radiation sources on product functionality as well as on the mechanical and optical properties of the constituent polymers. Results indicate that although measurable differences in properties between the standard gamma irradiated materials and the alternative E-beam and X-ray irradiated materials were observed, those differences were small. Statistically significant differences were noted in the case of yellowness index for polyvinyl chloride, high-density polyethylene, and polycarbonate, and in the case of tensile elongation at break for high impact polystyrene and polyvinyl chloride. In general, the results of this study support the viability of E-beam and X-ray radiation as alternative options to cobalt-60 gamma radiation for sterilization of Stryker single-use universal bone cement mixer medical devices.

Urinary hyaluronidase activity is closely related to vasopressinergic system following an oral water load in men: a potential role in blood pressure regulation and early stages of hypertension development.

Blood pressure (BP) regulation is a complex process involving several factors, among which water-sodium balance holds a prominent place. Arginin-vasopressin (AVP), a key player in water metabolism, has been evoked in hypertension development since the 1980s, but, to date, the matter is still controversial. Hyaluronic acid metabolism has been reported to be involved in renal water management, and AVP appears to increase hyaluronidase activity resulting in decreased high-molecular-weight hyaluronan content in the renal interstitium, facilitating water reabsorption in collecting ducts. Hence, our aim was to evaluate urinary hyaluronidase activity in response to an oral water load in hypertensive patients (HT, n=21) compared to normotensive subjects with (NT+, n=36) and without (NT-, n=29) a family history of hypertension, and to study its association with BP and AVP system activation, expressed by serum copeptin levels and urine Aquaporin 2 (AQP2)/creatinine ratio. Eighty-six Caucasian men were studied. Water load test consisted in oral administration of 15-20 ml of water/kg body weight over 40-45 min. BP, heart rate, serum copeptin, urine hyaluronidase activity and AQP2 were monitored for 4 hours. In response to water drinking, BP raised in all groups with a peak at 20-40 min. Baseline levels of serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio were similar among groups and all decreased after water load, reaching their nadir at 120min and then gradually recovering to baseline values. Significantly, a blunted reduction in serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio was observed in NT+ compared to NT- subjects. A strong positive correlation was also found between urinary hyaluronidase activity and AQP2/creatinine ratio, and, although limited to the NT- group, both parameters were positively associated with systolic BP. Our results demonstrate for the first time the existence in men of a close association between urinary hyaluronidase activity and vasopressinergic system and suggest that NT+ subjects have a reduced ability to respond to water loading possibly contributing to the blood volume expansion involved in early-stage hypertension. Considering these data, AVP could play a central role in BP regulation by affecting water metabolism through both hyaluronidase activity and AQP2 channel expression.

The impact of managers' attitudes towards environmental management accounting and green competitive advantage in Vietnam manufacturers

Environmental management accounting (EMA) has positive support for providing information for green competitive advantage. Based on upper echelons theory (UET), this study examines the impact of upper managers' attitudes towards the environment regarding the choice of green production strategy, EMA implementation, and green competitive advantage (GCA). The moderating role of regulatory pressure (RP) in some relationships is also investigated. This study, conducted in Vietnam, features a data set of 234 medium and large manufacturers. The sample is restricted to medium and large firms only, as these possess sufficient financial resources to operate independent marketing and management accounting functions. Partial Least Squares Structural Equation Modeling (PLS-SEM) is used to test hypotheses. We found that it has a significant positive and direct effect of attitude towards the environment on choosing a green production strategy and the implementation of EMA, thereby achieving a green competitive advantage. We also find that regulatory pressures have a moderator role and are positive in some relationships. This study applied UET in the management accounting field and presented the significance of the compatibility between upper managers’ attitudes towards the environment, strategies, and EMA implementation that would improve the long-term competitive advantage of the organizations.

Increased daytime and awakening salivary free aldosterone in essential hypertensive men.

While aldosterone plays an important role in blood pressure regulation, its role in essential hypertension (EHT) remains unclear. Here, we systematically investigated the secretion of biologically-active free aldosterone in saliva in response to awakening (AldAR) and during the day (AldDay) in EHT compared to normotensive controls (NT). In 30 men with EHT and 30 age-matched NT, AldAR saliva samples were collected immediately after awakening and 15, 30, 45, and 60 min thereafter and AldDay samples were collected from 08:30-22:00 h on two consecutive days. Over the course of the day, men with EHT had higher repeated AldDay levels compared to NT (p = .002) with higher concentrations in the morning hours (p's ≤ .047), a steeper decline over the course of the day (p's ≤ .018), and similar concentrations in the evening (p's ≥ .21). Regarding AldAR, we observed higher concentrations in EHT at awakening (p = .017) and borderline higher concentrations at 15 min (p = .086). No differences were found 30-60 min after awakening (p's ≥ .34). Analyses with repeated and aggregated AldAR levels resulted in borderline significantly higher free aldosterone in EHT (p's ≤ .077). Complementary analyses confirmed linear associations between higher blood pressure and higher AldAR and AldDay levels. Our data point to elevated salivary free aldosterone secretion in EHT over the course of the day, particularly in the morning hours. As the free aldosterone fraction is considered biologically active, our data may point to a biological mechanism underlying EHT.

Mass spectrometry coupling of chip-based supercritical fluid chromatography enabled by make-up flow-assisted backpressure regulation

This work introduces a novel microfluidic backpressure pressure control developed for chip-based supercritical fluid chromatography (chipSFC). The presented on-chip pressure control mechanism involves the post-column addition of a viscous make-up stream, which enables pressure regulation within the range of 73 to 130 bar range. In contrast to approaches using mechanical backpressure regulators, this chip-based make-up-assisted pressure regulation offers a wear-free alternative that functions entirely through fluidic means and contributes minimally to extra column volume. It prevents phase separation of the supercritical mobile phase and, therefore, expands the analytical scope of chipSFC to detection systems with an ambient pressure interface. This was demonstrated by a proof-of-principle experiment, where a model mixture was separated within 30 s and detected using atmospheric pressure ionisation mass spectrometry.Graphical abstract

Optimizing the connection of CRRT and ECMO lines with additional pressure regulator on the therapeutic effect, filter life, and incidence of complications.

Extracorporeal membrane oxygenation (ECMO) is used for severe cardiopulmonary failure, with veno-arterial ECMO for cardiogenic shock and veno-venous ECMO for acute respiratory failure. ECMO's application has expanded to ICUs, emergency departments, and operating rooms. ECMO patients are at high risk for complications, including acute kidney injury (AKI), often requiring renal replacement therapy (RRT), posing significant management challenges. From August 2015 to June 2022, 120 patients were cured with veno-venous ECMO (n = 60) or veno-arterial ECMO (VA-ECMO, n = 60) combined with CRRT in our hospital. In the control group (n = 60), the input end (arterial end) of CRRT was connected to the ECMO oxygenator. The reinfusion end (venous end) of CRRT was connected to the oxygenator of ECMO for CRRT + ECMO treatment. In the experimental group (n = 60), the input end (arterial end) of CRRT was connected to the oxygenator of ECMO, and an additional pressure regulating device was installed on the connection of the 2 lines. The observation indexes including clinical therapeutic effect, clinical therapeutic effect, the incidence of complications, and the incidence of complications were compared. There was a notable decrease in serum creatinine, and the differences in blood urea nitrogen, procalcitonin, and C-reactive protein after operation were statistically significant (P < .05). The filter use time in the study group was notably longer (P < .01). There exhibited no remarkable difference in the incidences of bleeding, thrombosis, numbness of hands and feet, metabolic alkalosis, disseminated intravascular coagulation, organ dysfunction syndrome, hyperbilirubinemia, and infection. This study demonstrates that additional pressure regulation devices are installed at the line connection between the CRRT input end and the CRRT return end to ensure that the flow rate of ECMO does not affect the CRRT treatment. ECMO and CRRT provide a safe pressure range so that the ECMO line can be safely connected to the CRRT machine at physiological pressure, reducing the occurrence of complications related to CRRT machine interruption and improving the efficiency of CRRT without affecting the efficiency of ECMO, ensuring patient safety.

Gene expression in heart, kidney, and liver identifies possible mechanisms underpinning fetal resistance and susceptibility to in utero PRRSV infection

Porcine reproductive and respiratory syndrome (PRRS) is one of the costliest diseases to pork producers worldwide. We tested samples from the pregnant gilt model (PGM) to better understand the fetal response to in-utero PRRS virus (PRRSV) infection. Our goal was to identify critical tissues and genes associated with fetal resilience or susceptibility. Pregnant gilts (N=22) were infected with PRRSV on day 86 of gestation. At 21 days post maternal infection, the gilts and fetuses were euthanized, and fetal tissues collected. Fetuses were characterized for PRRS viral load in fetal serum and thymus, and preservation status (viable or meconium stained: VIA or MEC). Fetuses (N=10 per group) were compared: uninfected (UNIF; <1 log/µL PRRSV RNA), resilient (HV_VIA, >5 log virus/µL but viable), and susceptible (HV_MEC, >5 log virus/µL with MEC). Gene expression in fetal heart, kidney, and liver was investigated using NanoString transcriptomics. Gene categories investigated were hypothesized to be involved in fetal response to PRRSV infection: renin- angiotensin-aldosterone, inflammatory, transporter and metabolic systems. Following PRRSV infection, CCL5 increased expression in heart and kidney, and ACE2 decreased expression in kidney, each associated with fetal PRRS susceptibility. Liver revealed the most significant differential gene expression: CXCL10 decreased and IL10 increased indicative of immune suppression. Increased liver gene expression indicated potential associations with fetal PRRS susceptibility on several systems including blood pressure regulation (AGTR1), energy metabolism (SLC16A1 and SLC16A7), tissue specific responses (KL) and growth modulation (TGFB1). Overall, analyses of non-lymphoid tissues provided clues to mechanisms of fetal compromise following maternal PRRSV infection.

Osmotic pressure regulated sodium alginate-graphene oxide hydrogel as a draw agent in forward osmosis desalination

The freshwater shortage has become a global issue due to the population explosion. As an effective response to the water crisis, forward osmosis (FO) desalination technology is applied because of its comparatively low membrane fouling and low energy consumption. Hydrogels, as draw solutions in FO, have received increasing attention due to their properties of no reverse solute flux, and strong water recovery capability. However, the concentration in the feed solution is below 8000 ppm restricted by the lack of a clear mechanism for osmotic pressure regulation. In this work, a sodium alginate-graphene oxide (SA-GO) hydrogel was proposed with excellent performance in drawing water. The hydrogel has a homogeneous porous structure that enables easy water recovery through compression. When the SA-GO-1 hydrogel was used for the treatment of the simulated seawater (35,000 ppm NaCl solutions), the initial water flux achieved 0.4429 L m−2 h−1 (LMH), and the average water flux remained at 0.1783LMH. In contrast to conventional tactics, the osmotic pressure of hydrogel was regulated based on Flory-Rehner theory (FR). Moreover, the interaction energy was computed to reveal the mechanism of osmotic pressure regulation. This strategy provides a potential solution for the design of high-performance draw solutions for FO systems.

Environmental regulation and labor demand: Evidence from China's air pollution prevention and Control Action Plan

In response to environmental regulatory pressure from the government, enterprises – as the main employers of labor – adjust their production-related choices and may alter their demand for labor. Numerous researches have probed into how environmental regulations affect the labor demand, which has emerged as a significant concern in the discourse around the adoption of environmental regulation policies. Nonetheless, many researches predominantly concentrates on the evaluation on the effects of environmental regulations on economic and environmental levels. Moreover, rare attention has been paid to how environmental regulations affect social activities, especially in terms of labor demands. As a result, the Air Pollution Prevention and Control Action Plan (APPCAP) is adopted as a “quasi-natural experiment” and a difference-in-differences model is employed to analyze the effects of the APPCAP on labor demands of companies. Hence, the 2008–2020 panel data are considered for the 3,949 Chinese A-share listed enterprises. Furthermore, this paper probes deeply into the underlying mechanisms based on mediation models. The following findings are thus concluded: (1) The labor demands of enterprises can be remarkably increased by APPCAP; and the result is still persuasive even though the endogeneity issues are taken into account. (2) APPCAP improves enterprise labor demand through the output and factor substitution effects. (3) As evidently revealed by heterogeneity analysis, the APPCAP could significant positive affect the labor demand size in state-owned companies, large-scale companies, new companies, and companies in polluting industries. (4) The APPCAP strikingly boosts the demand for labor force with high skills. Nevertheless, it exerts little influence on the demand for labor force with low skills and company salary levels. Therefore, the government must continue to steadfastly implement environmental regulatory policies but adopt different policies based on enterprise characteristics. Overall, this study provides micro-level experimental evidence for more in-depth understanding of how environmental policies affect labor market, which is particularly important for actively resolving social employment problems and exploring new growth points in enterprise employment.

Robust predictive fault-tolerant control under actuator random failures

A robust predictive fault-tolerant switching control method is presented for a class of industrial processes that are prone to random actuator failures. The primary contribution is the combination of stochastic control theory with robust predictive control to represent the occurrence of system failure in the form of probability, thus significantly enhancing the conventional fault-tolerant control approach. First, a state-space model of the system is established, which is then transformed into an enhanced form that includes state deviations and output tracking errors. This augmented model enables the formulation of fault-tolerant switching control laws with fault probabilities, ensuring system convergence and tracking capabilities. Subsequently, linear matrix inequality constraints are given to find the switching controller gains under recovery and fault probabilities. Under certain probabilities, failure triggers fault-tolerant control, while recovery prompts a shift back to conventional control. Final, we validate the feasibility of our approach with a case study involving pressure regulation.

Student academic representation in the UK: An exploration of recruitment, training, and impacts

AbstractStudent academic representation is a staple feature in UK and international higher education. It provides a vital quality function whereby students, who are elected representatives of their programme, canvas the opinions of peers to inform quality assurance processes. In the UK, there is increasing regulatory pressure for universities and student unions to be dual owners of representation activity and much work has been done to enact this model. Nonetheless, little is known about the experiences of those serving as student representatives, despite this being a community of thousands of students across the UK, who hold an instrumental position in institutional quality assurance. We developed an instrument (SARA) to evaluate experiences of 773 active student representatives from 15 UK HE institutions. It explored key areas, including recruitment, training, working approaches and development outcomes. Data indicated low engagement in training in some key areas (e.g., representation of diverse groups and data gathering) and a narrow range of working approaches. These patterns may compromise the value of student representation both from the institutional perspective and that of individual representatives seeking to enhance their skills. Recommendations are suggested for enhancing future practice.

Effects of DASH diet with or without time-restricted eating in the management of stage 1 primary hypertension: a randomized controlled trial

BackgroundTime-restricted eating (TRE), a popular form of intermittent fasting, has shown benefits for improving metabolic diseases and cardiometabolic health. However, the effect of TRE in the regulation of blood pressure in primary hypertension remains unclear.MethodsA 6-week randomized controlled trial was conducted, in which a total of 74 stage 1 primary hypertensive patients without high-risk were randomly assigned to Dietary Approaches to Stop Hypertension (DASH) group (n = 37) or DASH + TRE group (n = 37). Participants in the DASH + TRE group were instructed to consume their food within an 8-h window. Scientific research platform in We Chat application was used to track participants. The primary outcome was blood pressure. The secondary outcomes included body composition, cardiometabolic risk factors, inflammation-related parameters, urinary Na+ excretion, other clinical variables and safety outcomes.ResultsThe reduction of systolic blood pressure and diastolic blood pressure were 5.595 ± 4.072 and 5.351 ± 5.643 mm Hg in the DASH group and 8.459 ± 4.260 and 9.459 ± 4.375 mm Hg in the DASH + TRE group. DASH + TRE group improved blood pressure diurnal rhythm. Subjects in DASH + TRE group had decreased extracellular water and increased urinary Na+ excretion. Furthermore, the decrease in blood pressure was associated with a reduction of extracellular water or increase in urinary Na+ excretion. In addition, safety outcomes such as nighttime hunger were also reported.ConclusionOur study demonstrated that 8-h TRE + DASH diet caused a greater decrease in blood pressure in stage 1 primary hypertensive patients than DASH diet. This study may provide novel insights into the benefits of lifestyle modification in the treatment of primary hypertension.Trial registrationhttps://www.chictr.org.cn/ (ChiCTR2300069393, registered on March 15, 2023).

An endothelial regulatory module links blood pressure regulation with elite athletic performance.

The control of transcription is crucial for homeostasis in mammals. A previous selective sweep analysis of horse racing performance revealed a 19.6 kb candidate regulatory region 50 kb downstream of the Endothelin3 (EDN3) gene. Here, the region was narrowed to a 5.5 kb span of 14 SNVs, with elite and sub-elite haplotypes analyzed for association to racing performance, blood pressure and plasma levels of EDN3 in Coldblooded trotters and Standardbreds. Comparative analysis of human HiCap data identified the span as an enhancer cluster active in endothelial cells, interacting with genes relevant to blood pressure regulation. Coldblooded trotters with the sub-elite haplotype had significantly higher blood pressure compared to horses with the elite performing haplotype during exercise. Alleles within the elite haplotype were part of the standing variation in pre-domestication horses, and have risen in frequency during the era of breed development and selection. These results advance our understanding of the molecular genetics of athletic performance and vascular traits in both horses and humans.

Nexus between regulatory pressure, eco-friendly product demand and sustainable competitive advantage of manufacturing small and medium-sized enterprises: the mediating role of eco-innovation

AbstractAlthough previous research endeavours have extensively explored the environmental pressure-performance relationship, their findings have been inclusive. Therefore, this paper examines the direct and indirect (through the mediating role of eco-innovation) impact of regulatory pressure and eco-friendly product demand on sustainable competitive advantage. The research model was examined using Structural Equation Modeling methodology, analyzing data gathered from 183 senior managers within Egyptian SMEs. Several findings have been yielded, which are: (1) eco-innovation is positively motivated by regulatory pressure and eco-friendly product demand; (2) the direct link between environmental pressures (regulatory pressure, eco-friendly product demand) and sustainable competitive advantages is not statistically significant; and (3) eco-innovation acts as a mediator in the relationship between these environmental pressures and sustainable competitive advantage. This research theoretically contributes to the institutional theory of the firm and competitive advantages theory. Particularly, the findings of this research theoretically emphasize that eco-innovation plays a prominent role in alleviating and translating environmental pressures exerted on manufacturing SMEs into sustainable competitive advantages. Furthermore, the research provides practical implications, policy recommendations, limitations, and further research avenues.

Adverse impact of capital regulatory reform and policy remedy: theory and evidence

This paper studies the impact of capital regulatory reform on firm behavior. We develop a portfolio-choice model to investigate how capital regulatory reforms influence the risk-taking behavior of financial institutions with different capital adequacy levels. The model predicts that as regulation becomes more stringent, either all firms reduce their risk-taking, or there exists a capital-adequacy threshold below which risk-taking increases. The Chinese insurance solvency regulatory reform provides a unique natural experiment to examine firms’ risk-taking responses to the capital shock driven by the reform. We find that increasing regulatory pressure – i.e. a more stringent regulation – induces greater risk-taking for less capital-adequate insurers, the insurers whose risk-taking the regulator most wants to reduce. We rule out the potential reverse causality that insurers’ risk structures prior to the reform determine their degrees of capital shock and that insurers target low capital-adequate positions by taking more risks prior to the reform. Our results suggest that reinforcing the qualitative risk assessment, increasing the penalties of insolvent insurers, and increasing the risk sensitivity of capital requirements could be effective policy remedies for this backfiring problem.

Effect of an endothelial regulatory module on plasma proteomics in exercising horses

Elite performing exercise requires an intricate modulation of the blood pressure to support the working muscles with oxygen. We have previously identified a genomic regulatory module that associates with differences in blood pressures of importance for elite performance in racehorses. This study aimed to determine the effect of the regulatory module on the protein repertoire. We sampled plasma from 12 Coldblooded trotters divided into two endothelial regulatory module haplotype groups, a sub-elite performing haplotype (SPH) and an elite performing haplotype (EPH), each at rest and exercise. The haplotype groups and their interaction were interrogated in two analyses, i) individual paired ratio analysis for identifying differentially abundant proteins of exercise (DAPE) and interaction (DAPI) between haplotype and exercise, and ii) unpaired ratio analysis for identifying differentially abundant protein of haplotype (DAPH). The proteomics analyses revealed a widespread change in plasma protein content during exercise, with a decreased tendency in protein abundance that is mainly related to lung function, tissue fluids, metabolism, calcium ion pathway and cellular energy metabolism. Furthermore, we provide the first investigation of the proteome variation due to the interaction between exercise and related blood pressure haplotypes, which this difference was related to a faster switch to the lipoprotein and lipid metabolism during exercise for EPH. The molecular signatures identified in the present study contribute to an improved understanding of exercise-related blood pressure regulation.

Increased Expression of Orexin-A in Patients Affected by Polycystic Kidney Disease.

Orexin-A is a neuropeptide product of the lateral hypothalamus that acts on two receptors, OX1R and OX2R. The orexinergic system is involved in feeding, sleep, and pressure regulation. Recently, orexin-A levels have been found to be negatively correlated with renal function. Here, we analyzed orexin-A levels as well as the incidence of SNPs in the hypocretin neuropeptide precursor (HCRT) and its receptors, HCRTR1 and HCRTR2, in 64 patients affected by autosomal dominant polycystic kidney disease (ADPKD) bearing truncating mutations in the PKD1 or PKD2 genes. Twenty-four healthy volunteers constituted the control group. Serum orexin-A was assessed by ELISA, while the SNPs were investigated through Sanger sequencing. Correlations with the main clinical features of PKD patients were assessed. PKD patients showed impaired renal function (mean eGFR 67.8 ± 34.53) and a statistically higher systolic blood pressure compared with the control group (p < 0.001). Additionally, orexin-A levels in PKD patients were statistically higher than those in healthy controls (477.07 ± 69.42 pg/mL vs. 321.49 ± 78.01 pg/mL; p < 0.001). Furthermore, orexin-A inversely correlated with blood pressure (p = 0.0085), while a direct correlation with eGFR in PKD patients was found. None of the analyzed SNPs showed any association with orexin-A levels in PKD. In conclusion, our data highlights the emerging role of orexin-A in renal physiology and its potential relevance to PKD. Further research is essential to elucidate the intricate mechanisms underlying orexin-A signaling in renal function and its therapeutic implications for PKD and associated cardiovascular complications.