This study provides insights for enhancing the business climate in Ghana. The primary objective of the studywas to identify the most relevant binding constraint(s) on the activities of micro, small and medium-sizedenterprises (MSMEs) in the manufacturing sector of Sunyani Municipality and Berekum District of Ghana.Accordingly, a survey of 85 manufacturing MSMEs was conducted. In spite of energy crisis in Ghana at the timeof research, the most relevant binding constraints to the enterprises surveyed were poor access to technologyincluding technological support services, and poor access to market (which will be discussed in subsequentpaper). The findings contradict policy focus on addressing governance and regulatory bottlenecks, andinadequate physical infrastructure. This underscores the importance of local business climate surveys in additionto national surveys in establishing the right policy focus for enhancing the business climate of localities within acountry.