Growth Regulators Research Articles

Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava

The edible brown seaweed, Ecklonia cava, is highly valued for its bioactive compounds, and is widely used in food supplements and functional foods. The increasing demand for this seaweed in the food industry emphasizes the necessity for sustainable cultivation practices. This study focused on inducing callus in the meristem and stipe of E. cava using different culture media: Provasoli’s enriched seawater medium (PESI), enriched artificial seawater medium (ESAW), artificial enriched seawater medium (ASP2), or Von Stosch’s enriched seawater medium (VS). Various abiotic stress factors (photoperiod, agar concentration, and temperature), growth regulators, carbon sources, polyamines, and plasma treatments were explored for their impact on callus induction. Both stipe and meristem explants developed callus within three to six weeks across all media except ASP2. Callus development was favored at temperatures between 8 to 13 °C and in the absence of light. Stipe explants showed a higher callus induction rate (up to 65.59 ± 6.24%) compared to meristem (up to 57.53 ± 8.32%). Meristem explants showed optimal callus induction in PESI medium with a low concentration of indole-3-acetic acid (IAA; 40.93 ± 8.65%). However, higher concentrations of IAA and 1-naphthaleneacetic acid (NAA) reduced meristem callus induction. Stipe showed high induced-callus (up to 50.37 ± 5.17%) in PESI medium with low concentrations of IAA, NAA, and 6-benzylaminopurine (BAP). Both stipe and meristem explants induced largest callus at 2% sucrose, but higher carbon source concentrations reduced callus induction. Spermine (Spm) at 1 µM resulted in high induced calluses; however, increasing Spm concentrations decreased callus induction. This tissue culture technique not only supports mass cultivation of E. cava, but also holds potential for extending to other seaweed species, contributing to the sustainability of seaweed stocks for the food industry.

Identification of ARF genes in Juglans Sigillata Dode and analysis of their expression patterns under drought stress.

Auxin response factor (ARF), a transcription factors that controls the expression of genes responsive to auxin, plays a key role in the regulation of plant growth and development. Analyses aimed at identifying ARF family genes and characterizing their functions in Juglans sigillata Dode are lacking. We used bioinformatic approaches to identify members of the J. sigillata ARF gene family and analyze their evolutionary relationships, collinearity, cis-acting elements, and tissue-specific expression patterns. The expression patterns of ARF gene family members under natural drought conditions were also analyzed. The J. sigillata ARF gene family contained 31 members, which were unevenly distributed across 16 chromosomes. We constructed a phylogenetic tree of JsARF genes and other plant ARF genes. Cis-acting elements in the promoters of JsARF were predicted. JsARF28 showed higher expressions in both the roots and leaves. A heat map of the transcriptome data of the cluster analysis under drought stress indicated that JsARF3/9/11/17/20/26 are responsive to drought. The expression of the 11 ARF genes varied under PEG treatment and JsARF18 and JsARF20 were significantly up-regulated. The interactions between abiotic stresses and plant hormones are supported by our cumulative data, which also offers a theoretical groundwork for comprehending the ARF mechanism and drought resistance in J. sigillata.

Response of Dragon Fruit (Hylocereus sp.) Cuttings to Different Plant Growth Regulators.

Dragon fruit (Hylocereus sp.) is an exotic crop with a great potential for its cultivation in semi arid tracts throughout India. Its planting is mainly confined to harsh climates and degraded lands facing challenges in its establishment. Hence, availability of quality planting material is essential for attaining success. Due to long juvenile phase of the sexually propagated seedlings it can be propagated asexually by stem cuttings but proper rooting is not possible without exogenous auxin application. Hence, during 2022-2023 a research was carried out at the Horticultural Experimental area of Khalsa College, Amritsar to standardize the concentration of plant growth regulators viz. IBA, NAA and PHB for rooting and success rate in stem cuttings comprising of sixteen treatments with three replications arranged in randomized block design. The results revealed that the stem cuttings treated with IBA 4000 ppm proved to be superior for the sprouting and survival of the cuttings resulting in the improved vegetative growth with profuse, longer, thicker and the heaviest roots. Hence, the cutting treatment of IBA 4000 ppm can be proposed for dragon fruit plant propagation to meet the market demand in India.

Effect of Plant Growth Regulators on Economics and Harvest Index of Garlic (Allium sativum L.) under Terai region of West Bengal

Effect of Plant Growth Regulators on Economics and Harvest Index of Garlic (Allium sativum L.) under Terai region of West Bengal

Development of a Micropropagation Protocol for the Ex Situ Conservation of Nuttall's Scrub Oak (Quercus dumosa).

Worldwide, oak species are threatened with extinction due to habitat loss, pathogens, and changing fire regimes. Ex situ conservation through tissue culture may protect the remaining genetic diversity of Quercus dumosa, or the coastal sage scrub oak, from further loss. We designed three basal salt formulations based on the mineral composition of shoot tips and first leaves from mature Q. dumosa and explored carbohydrate source, stress-mitigating compounds, and plant growth regulator concentrations to develop a method of cultivating many Q. dumosa culture lines in vitro. All three novel basal salt formulations led to decreased necrosis compared with commercial basal salt formulas WPM, MS, and DKW. Substitution of 30 g L-1 sucrose with glucose and adding 250 mg L-1 ascorbic acid, 5.2 mg L-1 SNP sodium nitroprusside, and 103 mg L-1 y-aminobutyric acid improved culture health overall. In an experiment involving 115 culture lines, 0.66 mg L-1 6-benzylaminopurine produced the highest average shoots per explant, but 0.33 mg L-1 produced the greatest proportion of shoots 2 cm or greater. Incubation for 24 h in 20 mg L-1 indole-3-butyric acid led to the most rooting. These methods show promise for the ex situ conservation of many genotypes of endangered Q. dumosa.

Revealing the Complete Bispecific Phosphatase Genes (DUSPs) across the Genome and Investigating the Expression Patterns of GH_A11G3500 Resistance against Verticillium wilt.

DUSPs, a diverse group of protein phosphatases, play a pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate in the MAPK pathway, which governs crucial aspects of plant physiology, including growth regulation, disease resistance, pest resistance, and stress response. DUSP is a key enzyme, and it is the enzyme that limits the rate of cell metabolism. At present, complete understanding of the DUSP gene family in cotton and its specific roles in resistance to Verticillium wilt (VW) remains elusive. To address this knowledge gap, we conducted a comprehensive identification and analysis of four key cotton species: Gossypium arboreum, Gossypium barbadense, Gossypium hirsutum, and Gossypium raimondii. The results revealed the identification of a total of 120 DUSP genes in the four cotton varieties, which were categorized into six subgroups and randomly distributed at both ends of 26 chromosomes, predominantly localized within the nucleus. Our analysis demonstrated that closely related DUSP genes exhibited similarities in terms of the conserved motif composition and gene structure. A promoter analysis performed on the GhDUSP gene promoter revealed the presence of several cis-acting elements, which are associated with abiotic and biotic stress responses, as well as hormone signaling. A tissue expression pattern analysis demonstrated significant variations in GhDUSP gene expression under different stress conditions, with roots exhibiting the highest levels, followed by stems and leaves. In terms of tissue-specific detection, petals, leaves, stems, stamens, and receptacles exhibited higher expression levels of the GhDUSP gene. The gene expression analysis results for GhDUSPs under stress suggest that DUSP genes may have a crucial role in the cotton response to stress in cotton. Through Virus-Induced Gene Silencing (VIGS) experiments, the silencing of the target gene significantly reduced the resistance efficiency of disease-resistant varieties against Verticillium wilt (VW). Consequently, we conclude that GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW). This study presents a comprehensive structure designed to provide an in-depth understanding of the potential biological functions of cotton, providing a strong foundation for further research into molecular breeding and resistance to plant pathogens.

Exogenous application of bio-stimulants and growth retardants improve nutrient absorption and fiber quality in upland cotton

BackgroundNatural and synthetic plant growth regulators are essential for plant health, likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stability. In the present study, we have evaluated the effects of foliar applied plant growth regulators, i.e., moringa leaf extract (MLE) and mepiquat chloride (MC) alone and in combination MC and MLE on the conventional cotton cultivar (CIM 573) and transgenic one (CIM 598). The growth regulators were applied at the start of bloom, 45 and 90 days after blooming.ResultsThe application of MC and MLE at 90 days after blooming significantly improved the relative growth rate, net assimilation rate, the number of bolls per plant, and seed cotton yield. Likewise, the combined application of MLE and MC at 90 days after blooming significantly boosted the nitrogen uptake in locules, as well as the phosphorus and potassium uptake in the leaves of both cotton cultivars. The application of MLE alone has considerably improved the nitrogen uptake in leaves, and phosphorus and potassium contents in locules of Bt and conventional cotton cultivars. Similarly, Bt cotton treated with MLE at 90 days after blooming produced significantly higher ginning out turn and oil contents. Treatment in combination (MLE + MC) at 90 days after blooming produced considerably higher micronaire value, fiber strength, and staple length in conventional cultivar.ConclusionThe natural growth enhancer, MLE is a rich source of minerals and zeatin, improving the nutrient absorption and quality of cotton fiber in both conventional and Bt cotton cultivars.

BRZ-INSENSITIVE-LONG HYPOCOTYL8 inhibits kinase-mediated phosphorylation to regulate brassinosteroid signaling.

Glycogen synthase kinase 3 (GSK3) is an evolutionarily conserved serine/threonine protein kinase in eukaryotes. In plants, the GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2) functions as a central signaling node through which hormonal and environmental signals are integrated to regulate plant development and stress adaptation. BIN2 plays a major regulatory role in brassinosteroid (BR) signaling and is critical for phosphorylating/inactivating BRASSINAZOLE-RESISTANT 1 (BZR1), also known as BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1), a master transcription factor of BR signaling, but the detailed regulatory mechanism of BIN2 action has not been fully revealed. In this study, we identified BIL8 as a positive regulator of BR signaling and plant growth in Arabidopsis (Arabidopsis thaliana). Genetic and biochemical analyses showed that BIL8 is downstream of the BR receptor BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and promotes the dephosphorylation of BIL1/BZR1. BIL8 interacts with and inhibits the activity of the BIN2 kinase, leading to the accumulation of dephosphorylated BIL1/BZR1. BIL8 suppresses the cytoplasmic localization of BIL1/BZR1, which is induced via BIN2-mediated phosphorylation. Our study reveals a regulatory factor, BIL8, that positively regulates BR signaling by inhibiting BIN2 activity.

Role of [N2222][Arg] amino acid ionic liquids in the enhancement of biomass in Haematococcus pluvialis via the regulation of reactive oxygen species signals

The slow growth of Haematococcus pluvialis restricts its use for large-scale production of astaxanthin. In this study, [N2222][Arg] amino acid ionic liquids (AAILs) were applied to increase the biomass of H. pluvialis. The maximum biomass concentration and cell count reached 0.98 g L−1 and 165.3 × 104 cells mL−1, respectively. An increase of 38.03% in biomass and 34.31% in cell number was observed following supplementation with [N2222][Arg]. Treatment with [N2222][Arg] resulted in increases in carbohydrate, protein and chlorophyll contents, Fv/Fm and the expression levels of genes associated with cell growth. Notably, [N2222][Arg] application triggered reactive oxidative species (ROS) and lateral membrane diffusion and changed the fatty acid composition, which is potentially involved in cell division and growth. Further data indicated that exogenous H2O2 could enhance alga biomass production in the presence of the [N2222][Arg] ionic liquid. Moreover, the accumulation of astaxanthin also increased in H. pluvialis treated with [N2222][Arg]. This study presents a potential method for enhancing the biomass production of H. pluvialis using amino acid ionic liquids and elucidates the role of ROS signalling in the regulation of cell growth.

PENGARUH PEMBERIAN ZAT PERANGSANG TUMBUH TERHADAP PERTUMBUHAN BIBIT TANAMAN GAHARU (Aquilaria malaccensis Lamk) DI POLYBAG

This research was carried out in Penginjau Hamlet, Bathin II Pelayang District, Bungo Regency, at an altitude of ± 75 meters above sea level. The research was carried out from 05 January 2015 to 05 April 2015. This research aims to study to determine the effect of giving growth stimulants on the growth of gaharu plant seeds (Aquilaria malaccensis Lamk) in polybags. The aim of the research is to obtain a dose of growth stimulants that will give results to the growth of agarwood seedlings.The method used in this research was a completely randomized design (RAK), with 5 treatments and 4 replications. The concentration of Atonic growth stimulant used was as follows: A0: No treatment (as control), A1: 0.50 cc Atonic ZPT/liter of water , A2: 1.00 cc ZPT Atonik/ liter of water, A3: 1.50 cc ZPT Atonik/ liter of water and A4: 2.00 cc ZPT Atonik/ liter of water. The data obtained from the final observations were analyzed using variance, if they had a real effect then continued with the Duncan New's Multiple Range Test (DNMRT). The variables observed were plant height (cm), total leaf area (cm2), stem diameter (mm) and root volume (ml). From the experimental results, the administration of atonic plant growth regulator (ZPT) concentrations had no significant effect on plant height (cm), total leaf area (cm2), stem circumference (mm) and root volume (ml)

An in silico drug repurposing approach to identify HDAC1 inhibitors against glioblastoma

Despite considerable improvement in therapy and diagnosis, brain tumors remain a global public health concern. Among all brain tumors, 80% are due to Glioblastoma. The average survival rate of a patient once diagnosed with glioblastoma is 15 months. Lately, the role of peptidase enzymes, especially Neprilysin, a neutral endopeptidase, is gaining attention for its role in tumor growth regulation. Neprilysin expressions are positively correlated with several tumors including GBM and reduced expression of NEP protein is associated with the pathogenesis of multiple tumors. One of the main reasons for NEP protein downregulation is the action of Histone deacetylase (HDAC) enzymes, especially HDAC1. Additionally, studies have reported that increased levels of HDAC1 are responsible for downregulating NEP gene expression. Hence, HDAC1 inhibition can be a good target to elevate NEP levels, which can be a good therapeutic approach to GBM. This study utilizes the computational drug repurposing tool, Schrodinger Maestro to identify HDAC1 inhibitors from the ZINC15 database.1379 FDA-approved drugs from the ZINC15 database were screened through molecular docking. Based on docking score and ligand-protein interaction, the top ten molecules were selected which were then subjected to binding energy calculation and molecular dynamics (MD) simulations. The three most active drugs from the MD simulations- ZINC22010649 (Panobinostat), ZINC4392649 (Tasimelteon) and ZINC1673 (Melphalan), were tested on C6 and U87 MG glioblastoma cells for cytotoxicity and HDAC1 protein levels using western blot analysis. Among the three drugs, Panobinostat exhibited potent cytotoxic action and showed a significant reduction in the HDAC1 protein levels. Communicated by Ramaswamy H. Sarma

Identification of a new class of activators of the Hippo pathway with antitumor activity in vitro and in vivo

The Hippo pathway is a key regulator of tissue growth, organ size, and tumorigenesis. Activating the Hippo pathway by gene editing or pharmaceutical intervention has been proven to be a new therapeutic strategy for treatment of the Hippo pathway-dependent cancers. To now, a number of compounds that directly target the downstream effector proteins of Hippo pathway, including YAP and TEADs, have been disclosed, but very few Hippo pathway activators are reported. Here, we discovered a new class of Hippo pathway activator, YL-602, which inhibited CTGF expression in cells irrespective of cell density and the presence of serum. Mechanistically, YL-602 activates the Hippo pathway via MST1/2, which is different from known activators of Hippo pathway. In vitro, YL-602 significantly induced tumor cell apoptosis and inhibited colony formation of tumor cells. In vivo, oral administration of YL-602 substantially suppressed the growth of cancer cells by activation of Hippo pathway. Overall, YL-602 could be a promising lead compound, and deserves further investigation for its mechanism of action and therapeutic applications.

Brassinosteroids: Relevant Evidence Related to Mitigation of Abiotic and Biotic Stresses in Plants

Extreme events of climate change are increasing, such as droughts and heat waves, causing limitations on growth and yield in relevant food crops, as well as threatening global food security. Brassinosteroids (BRs) are natural or synthetic steroids with significant properties that promote plant growth and development. In the current world scenario, research and solutions that can improve plant tolerance to climate change are strategic to ensure food security. The distinctiveness and novelty of this review lie in its comprehensive and detailed approach to the role of BRs in plants under biotic and abiotic stresses. We consolidate information on the action mechanisms on specific organs, providing detailed experimental conclusions of these plant growth regulators, including also commercial products and concentrations tested aiming to mitigate the adverse effects of the stresses. This practical approach highlights the potential of BRs in agriculture and plant protection against stresses. Additionally, our review presents results with plant models and essential food crops, focusing on multidisciplinary approaches and using physiological, biochemical, nutritional, anatomical and agronomic tools to explain the mechanisms of action of brassinosteroids in plants exposed to abiotic and biotic stresses.

Setting up Agrobacterium tumefaciens-mediated transformation of the tropical legume Aeschynomene evenia, a powerful tool for studying gene function in Nod Factor-independent symbiosis.

Most legumes are able to develop a root nodule symbiosis in association with proteobacteria collectively called rhizobia. Among them, the tropical species Aeschynomene evenia has the remarkable property of being nodulated by photosynthetic Rhizobia without the intervention of Nod Factors (NodF). Thereby, A. evenia has emerged as a working model for investigating the NodF-independent symbiosis. Despite the availability of numerous resources and tools to study the molecular basis of this atypical symbiosis, the lack of a transformation system based on Agrobacterium tumefaciens significantly limits the range of functional approaches. In this report, we present the development of a stable genetic transformation procedure for A. evenia. We first assessed its regeneration capability and found that a combination of two growth regulators, NAA (= Naphthalene Acetic Acid) and BAP (= 6-BenzylAminoPurine) allows the induction of budding calli from epicotyls, hypocotyls and cotyledons with a high efficiency in media containing 0,5 μM NAA (up to 100% of calli with continuous stem proliferation). To optimize the generation of transgenic lines, we employed A. tumefaciens strain EHA105 harboring a binary vector carrying the hygromycin resistance gene and the mCherry fluorescent marker. Epicotyls and hypocotyls were used as the starting material for this process. We have found that one growth medium containing a combination of NAA (0,5 μM) and BAP (2,2 μM) was sufficient to induce callogenesis and A. tumefaciens strain EHA105 was sufficiently virulent to yield a high number of transformed calli. This simple and efficient method constitutes a valuable tool that will greatly facilitate the functional studies in NodF-independent symbiosis.

Maternal Education is a Major Factor in Growth Regulation in Twins and Singletons

Maternal Education is a Major Factor in Growth Regulation in Twins and Singletons

The small iron-deficiency-induced protein OLIVIA and its relation to the bHLH transcription factor POPEYE.

Iron (Fe) is a crucial micronutrient needed in many metabolic processes. To balance needs and potential toxicity, plants control the amount of Fe they take up and allocate to leaves and seeds during their development. One important regulator of this process is POPEYE (PYE). PYE is a Fe deficiency-induced key bHLH transcription factor (TF) for allocation of internal Fe in plants. In the absence of PYE, there is altered Fe translocation and plants develop a leaf chlorosis. NICOTIANAMINE SYNTHASE4 (NAS4), FERRIC-REDUCTION OXIDASE3 (FRO3), and ZINC-INDUCED FACILITATOR1 (ZIF1) genes are expressed at higher level in pye-1 indicating that PYE represses these genes. PYE activity is controlled in a yet unknown manner. Here, we show that a small Fe deficiency-induced protein OLIVIA (OLV) can interact with PYE. OLV has a conserved C-terminal motif, that we named TGIYY. Through deletion mapping, we pinpointed that OLV TGIYY and several regions of PYE can be involved in the protein interaction. An OLV overexpressing (OX) mutant line exhibited an enhanced NAS4 gene expression. This was a mild Fe deficiency response phenotype that was related to PYE function. Leaf rosettes of olv mutants remained smaller than those of wild type, indicating that OLV promotes plant growth. Taken together, our study identified a small protein OLV as a candidate that may connect aspects of Fe homeostasis with regulation of leaf growth.

Enhancing carbon emission performance in resource-based cities through technological innovation under China’s digital economic development strategy

As indispensable contributors to the energy and resource sectors, resource-based cities have played a pivotal role in shaping China’s carbon emission landscape. The carbon emission performance of these cities directly impacts the overall carbon footprint of the country, thus highlighting the significance of enhancing carbon emission efficiency within resource-based urban areas to drive nationwide carbon reduction initiatives effectively. The digital economic development strategy presents a promising avenue for improving carbon emission efficiency in resource-based cities. This study employs the super efficiency epsilon-based measure model to assess the carbon emission performance of 103 resource-based cities over the period from 2011 to 2019. Additionally, it utilizes an intermediate effect model to analyse the impact mechanism of digital economic development on carbon emission performance. The findings reveal a substantial 6.3% enhancement in carbon emission performance in resource-based cities attributed to the implementation of the digital development strategy, primarily driven by technological innovation. Furthermore, the study identifies significant dual-threshold effects within economic growth and environmental regulation, shedding light on the complex interplay between these factors. These insights offer valuable guidance for regional carbon reduction endeavors and serve as a valuable supplement to the understanding of the environmental implications of digital economic development in resource-based cities.

Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana.

Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth and development through controlling plasma membrane protein composition and cargo uptake. CME relies on the precise recruitment of regulators for vesicle maturation and release. Homologues of components of mammalian vesicle scission are strong candidates to be part of the scission machinery in plants, but the precise roles of these proteins in this process are not fully understood. Here, we characterised the roles of the plant dynamin-related protein 2 (DRP2) family (hereafter DRP2s) and SH3-domain containing protein 2 (SH3P2), the plant homologue to recruiters of dynamins, such as endophilin and amphiphysin, in CME by combining high-resolution imaging of endocytic events in vivo and characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive similarly late during CME and physically interact, genetic analysis of the sh3p123 triple mutant and complementation assays with non-SH3P2-interacting DRP2 variants suggest that SH3P2 does not directly recruit DRP2s to the site of endocytosis. These observations imply that, despite the presence of many well-conserved endocytic components, plants have acquired a distinct mechanism for CME.

Exogenous Spermidine Alleviated Low-Temperature Damage by Affecting Polyamine Metabolism and Antioxidant Levels in Apples.

Low-temperature stress significantly limits the growth, development, and geographical distribution of apple cultivation. Spermidine (Spd), a known plant growth regulator, plays a vital role in the plant's response to abiotic stress. Yet, the mechanisms by which exogenous Spd enhances cold resistance in apples remain poorly understood. Therefore, the present study analyzed the effects of exogenous Spd on antioxidant enzyme activity, polyamine metabolism, and related gene expression levels of 1-year-old apple branches under low-temperature stress. Treatment with exogenous Spd was found to stabilize branch tissue biofilms and significantly reduce the levels of reactive oxygen species by elevating proline content and boosting the activity of antioxidants such as superoxide dismutase. It also upregulated the activities of arginine decarboxylase, S-adenosylmethionine decarboxylase, and spermidine synthase and the expression levels of MdADC1, MdSAMDC1, and MdSPDS1 under low-temperature stress and led to the accumulation of large amounts of Spd and spermine. Moreover, compared with the 2 mmol·L-1 Spd treatment, the 1 mmol·L-1 Spd treatment increased the expression levels of cold-responsive genes MdCBF1/2/3, MdCOR47, and MdKIN1, significantly. The findings suggest that exogenous Spd can enhance cold resistance in apple branches significantly. This enhancement is achieved by modulating polyamine metabolism and improving antioxidant defense mechanisms, which could be exploited to improve apple cultivation under cold stress conditions.

Study on Tissue Culture and Rapid Propagation Techniques of Different Explants of Codonopsis convolvulacea Kurz

The tissue culture technology of Dioscorea bulbifera L. is of great significance for the effective protection and utilization of its germplasm resources. This paper reviews the research progress of tissue culture technology of Dioscorea bulbifera L. with different explants. It introduces the historical development of tissue culture technology of Dioscorea bulbifera L., including early exploration and gradual improvement of modern technology. The paper elaborates various methods and strategies of tissue culture technology of Dioscorea bulbifera L. at present, including research progress in aspects such as explant selection, medium formulation, and application of growth regulators. It also looks forward to the future development direction and potential applications of tissue culture technology of Dioscorea bulbifera L., and discusses its application prospects in germplasm resource preservation, new variety breeding, and improvement of disease and pest resistance. This paper is of important theoretical and practical significance for promoting the research and application of tissue culture technology of Dioscorea bulbifera L.